Article
Multidisciplinary Sciences
Justine Lengrand, Ievgenia Pastushenko, Sebastiaan Vanuytven, Yura Song, David Venet, Rahul M. Sarate, Melanie Bellina, Virginie Moers, Alice Boinet, Alejandro Sifrim, Nicolas Rama, Benjamin Ducarouge, Jens Van Herck, Christine Dubois, Samuel Scozzaro, Sophie Lemaire, Sarah Gieskes, Sophie Bonni, Amandine Collin, Nicolas Braissand, Justine Allard, Egor Zindy, Christine Decaestecker, Christos Sotiriou, Isabelle Salmon, Patrick Mehlen, Thierry Voet, Agnes Bernet, Cedric Blanpain
Summary: Epithelial-to-mesenchymal transition (EMT) plays a crucial role in cancer initiation, progression, metastasis, and resistance to therapy. In this study, the upregulation of netrin-1 in skin squamous cell carcinoma (SCC) was found to induce EMT, and pharmacological inhibition of netrin-1 decreased EMT and enhanced chemotherapy sensitivity. These findings suggest a potential therapeutic strategy for targeting EMT in cancer.
Article
Biochemistry & Molecular Biology
Nan Qiu, Daniel Abegg, Mara Guidi, Kerry Gilmore, Peter H. Seeberger, Alexander Adibekian
Summary: Protein S-palmitoylation is crucial in cancer cells, and artemisinin, a clinically approved antimalarial drug, has been found to inhibit a specific palmitoyl transferase, reducing palmitoylation of oncogenic proteins and disrupting cell proliferation signaling cascades.
CELL CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luciano Nicosia, Francesca Ludovica Boffo, Elena Ceccacci, Fabio Conforti, Isabella Pallavicini, Fabio Bedin, Roberto Ravasio, Enrico Massignani, Tim C. P. Somervaille, Saverio Minucci, Tiziana Bonaldi
Summary: LSD1 inhibitors MC2580 and DDP-38003 induce AML cell differentiation through downregulation of GSE1 protein, leading to decreased cell viability in vitro and tumor growth in vivo. These inhibitors also upregulate genes associated with immune response and cytokine-signaling pathways by reducing GSE1 binding to their promoters, thus activating transcriptional programs that trigger myeloid differentiation.
Article
Cell Biology
Charles-Antoine Assenmacher, Sara F. Santagostino, Mark A. Oyama, Jean-Christophe Marine, Elise Bonvin, Enrico Radaelli
Summary: This study focused on the classification and grading of cutaneous and lymph nodal melanocytic proliferations in Tg(Tyr-NRAS*Q61K)1Bee mice, using histopathology and expression analysis. It was found that high-grade lesions showed significantly increased expression of Ki-67, MITF, gp100, and nestin compared to low-grade lesions, establishing a linear correlation between tumor grade and these biomarkers. MITF IHC assessment accuracy was also confirmed. Incorporating these analyses into the classification scheme for melanocytic proliferations in this model may improve accuracy in assessing phenotype, disease progression, and predicting treatment response.
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2021)
Article
Chemistry, Medicinal
Xiu-Cai Chen, Gui-Xue Tang, Jing Dai, Le-Tian Dai, Tian-Ying Wu, Wen-Wei Li, Tian-Miao Ou, Zhi-Shu Huang, Jia-Heng Tan, Shuo-Bin Chen
Summary: Mutations in NRAS contribute to tumor formation and resistance to drugs. The development of novel strategies to suppress NRAS for anticancer therapy is urgent, as this protein is usually considered untargetable. Recent research has shown that a G-quadruplex structure in the untranslated region of NRAS mRNA can reduce NRAS translation, providing a potential NRAS suppression strategy. In this study, a cell-based screening method was developed using a G-quadruplex-triggered fluorogenic hybridization probe, and the clinically used agent Octenidine was identified as a potent NRAS repressor. Octenidine inhibited NRAS translation, blocked MAPK and PI3K-AKT signaling, and induced cell cycle arrest, apoptosis, and autophagy. It exhibited better antiproliferative effects compared to clinical antimelanoma agents and could inhibit the growth of NRAS-mutant melanoma in a mouse model. These results suggest that Octenidine may be a promising therapy for NRAS-mutant melanoma.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Oncology
Atreyi Dasgupta, Laura Sierra, Susan Tsang, Lyazat Kurenbekova, Tajhal Patel, Kimal Rajapakshe, Ryan L. Shuck, Nino Rainusso, Yosef Landesman, Thaddeus Unger, Cristian Coarfa, Jason T. Yustein
Summary: The study identifies PAK4 as a novel and viable therapeutic target for high-risk RMS, showing inhibition of PAK4 activity as a promising viable therapeutic option for advanced rhabdomyosarcoma.
Article
Biochemistry & Molecular Biology
Yuya Monoe, Kentaro Jingushi, Akitaka Kawase, Takayuki Hirono, Ryo Hirose, Yoshino Nakatsuji, Kaori Kitae, Yuko Ueda, Hiroaki Hase, Yuichi Abe, Jun Adachi, Takeshi Tomonaga, Kazutake Tsujikawa
Summary: The newly designed LNA targeting the miR-130 family could inhibit the growth, invasion, and migration of bladder cancer cells by targeting protein tyrosine phosphatase non-receptor type 1 (PTPN1) and downstream signaling pathways, providing a promising approach for the treatment of bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Annamil Alvarez-Trotta, William Guerrant, Luisana Astudillo, Mohini Lahiry, Giulia Diluvio, Elena Shersher, Hugo Kaneku, David J. Robbins, Darren Orton, Anthony J. Capobianco
Summary: NADI-351 is the first specific small-molecule inhibitor that selectively disrupts Notch1 transcriptional complexes, demonstrating robust antitumor activity without inducing intestinal toxicity, providing a potential therapeutic approach for improved cancer treatment.
Article
Oncology
Chaoqun Liu, Zhihua Pan, Qian Chen, Zetao Chen, Weiwei Liu, Ling Wu, Muhong Jiang, Wandie Lin, Yujie Zhang, Weihao Lin, Rui Zhou, Liang Zhao
Summary: The study found that PTK6 is overexpressed in CRC tissues and promotes the stemness and chemoresistance of CRC cells by activating the JAK2/STAT3 signaling pathway. Pharmacological inhibition of PTK6 using XMU-MP-2 significantly reduces stemness property and enhances chemosensitivity in CRC cells.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Medicine, Research & Experimental
Beatriz Parejo-Alonso, Alba Royo-Garcia, Pilar Espiau-Romera, Sarah Courtois, Alvaro Curiel-Garcia, Sladjana Zagorac, Isabel Villaoslada, Kenneth P. Olive, Christopher Heeschen, Patricia Sancho
Summary: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease characterized by metastasis and chemoresistance. Targeting the ALK signaling pathway can decrease tumor formation and chemoresistance in PDAC by inhibiting pancreatic cancer stem cells.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Anmol Sharma, Heena Khan, Thakur Gurjeet Singh, Amarjot Kaur Grewal, Agnieszka Najda, Malgorzata Kawecka-Radomska, Mohamed Kamel, Ahmed E. Altyar, Mohamed M. Abdel-Daim
Summary: The ubiquitin-proteasome pathway is crucial in regulating various biological functions and plays a significant role in tumor development. Inhibiting specific enzymes within this pathway can be an effective treatment for cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Aushia Tanzih Al Haq, Hong-Yu Tseng, Li-Mei Chen, Chien-Chia Wang, Hsin-Ling Hsu
Summary: Triple-negative breast cancer (TNBC) and the aggressive subtypes of HER2(+) breast cancer are associated with high expression levels of mitochondrial superoxide dismutase (MnSOD). The oncoprotein MCT-1 induces MnSOD in TNBC cells by stabilizing the transcription factor Nrf2. High expression of MnSOD correlates with poor prognosis in breast cancer patients.
CELL DEATH & DISEASE
(2022)
Review
Chemistry, Multidisciplinary
Yue Huang, Wenyang Xia, Ze Dong, Cai-Guang Yang
Summary: Epigenetics, including the N-6-methyladenosine (m(6)A) modification, plays a critical role in gene expression regulation. Understanding the biology of RNA epigenetics and developing drugs targeting m(6)A modifying proteins is important for cancer research and therapy.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Oncology
Lei Jin, Xuemei Ma, Nan Zhang, Qian Zhang, Xueming Chen, Zhongtao Zhang, Guoqian Ding, Hongzhi Yu
Summary: miR-181a-2-3p was identified as a unique miRNA among the common differentially expressed miRNAs in three DDP-resistant and DDP-sensitive gastric cancer cell lines. It exhibited higher expression in larger tumors, higher T stage, and chemotherapy-resistant patients. miR-181a-2-3p could differentiate between chemosensitive and chemoresistant GC patients and serve as a valid biomarker for predicting overall survival. Upregulation of miR-181a-2-3p enhanced the effects of DDP, while downregulation decreased these effects, indicating its potential as a therapeutic target and tumor biomarker for gastric cancer.
CANCER MANAGEMENT AND RESEARCH
(2021)
Article
Biology
Lisa Goebel, Tonia Kirschner, Sandra Koska, Amrita Rai, Petra Janning, Stefano Maffini, Helge Vatheuer, Paul Czodrowski, Roger S. Goody, Matthias P. Mueller, Daniel Rauh
Summary: In this study, we developed nucleotide-based covalent inhibitors for the oncogenic mutant KRasG13C, which has been difficult to target in the past. These inhibitors showed promising properties and successfully locked KRasG13C in a crystal structure. Importantly, the modified KRasG13C protein with these inhibitors was unable to undergo nucleotide exchange and induce oncogenic signaling in cells, suggesting the potential use of nucleotide-based inhibitors for KRasG13C-driven cancer.
Article
Computer Science, Artificial Intelligence
Fei Tan, Changwei Hu, Yifan Hu, Kevin Yen, Zhi Wei, Aasish Pappu, Serim Park, Keqian Li
Summary: This work addresses two challenges in text moderation: multibyte character representation and word obfuscations. The author proposes a multihot byte-level scheme to reduce dimensionality and a weighting approach for fusing n-gram features. Surprisingly, it outperforms neural networks and an enhanced variant is developed to handle intentional word obfuscations. The comprehensive experiments demonstrate significant performance improvements on abusive language datasets.
IEEE TRANSACTIONS ON NEURAL NETWORKS AND LEARNING SYSTEMS
(2023)
Article
Plant Sciences
Min Jiang, Xiaoying Wang, Bin Lv, Yujie Lu, Xianghui Ma, Wenjuan Liu, Gang Bai, Xiumei Gao
Summary: The deposition of psoralen in the liver is the primary cause of liver damage, which is related to the inhibition of CYPs and GSTs activities leading to the depletion of GSH. Co-administration of GSH and psoralen is an effective approach to prevent liver injury in clinical settings.
Article
Plant Sciences
Hui Na Cao, Jing Ya Ruan, Yu Han, Wei Zhao, Ying Zhang, Chang Gao, Hong Hua Wu, Lin Ma, Xiu Mei Gao, Yi Zhang, Tao Wang
Summary: The flavonoid glycosides from A. wilsonii seeds exhibit anti-inflammatory effects by inhibiting the release of nitric oxide through multiple signaling pathways.
Article
Biochemical Research Methods
Li Jia, Hongda Wang, Xiaoyan Xu, Huimin Wang, Xue Li, Ying Hu, Boxue Chen, Meiyu Liu, Xiumei Gao, Huifang Li, Dean Guo, Wenzhi Yang
Summary: Comprehensively elucidating herbal metabolites is crucial in natural products research for the discovery of new lead compounds. This study presents an offline multidimensional chromatography/high-resolution mass spectrometry approach which successfully identified a large number of ginsenosides from the flower buds of Panax plants, including potentially unknown compounds.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Dongxue Zhao, Meiyu Liu, He Sun, Xiaoyan Xu, Simiao Wang, Hongda Wang, Xue Li, Meiting Jiang, Boxue Chen, Yuying Zhao, Xiumei Gao, Wenzhi Yang
Summary: Addressing chemical complexity is crucial in various research fields. The integration of multidimensional chromatography and mass spectrometry enables comprehensive characterization of herbal metabolites. In this study, a three-dimensional liquid chromatography system combined with quadrupole time-of-flight mass spectrometry was used to fully characterize the metabolome of two Astragalus species. By utilizing an in-house library and reference standards, a total of 513 compounds from the Astragalus species were identified. This approach allows for in-depth characterization of herbal metabolites.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Plant Sciences
Xiaoyan Qin, Qiu Wei, Ran An, Yun Yang, Mingqi Cai, Xiaoling Han, Haoping Mao, Xiumei Gao
Summary: This study investigates the anti-osteoporotic effects of an ethanol extract of FLL in ovariectomized mice and explores the underlying mechanism. The results show that FLL prevents bone loss and improves bone microstructure by modulating bone and fat balance, suggesting its potential as a therapeutic agent for postmenopausal osteoporosis.
PHARMACEUTICAL BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Zhihao Liu, Xiaozhi Liu, Li Liu, Ying Wang, Jie Zheng, Lan Li, Sheng Li, Han Zhang, Jingyu Ni, Chuanrui Ma, Xiumei Gao, Xiyun Bian, Guanwei Fan
Summary: SUMOylation is a dynamic post-translational modification that maintains cardiac function and protects against hypertrophic response in the heart. The role of SUMO1 in myocardial infarction (MI) and the molecular details of heart cell responses to SUMO1 deficiency were investigated in this study. SUMO1 knockout worsened systolic dysfunction and infarct size after myocardial injury, and its loss promoted proliferation of endothelial cell subsets with angiogenic potential.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Pharmacology & Pharmacy
Bin Lv, Jinhe Guo, Yaodong Du, Yang Chen, Xin Zhao, Bin Yu, Jiarui Liu, Tianyi Cui, Haoping Mao, Xiaoying Wang, Xiumei Gao
Summary: Respiratory diseases, such as acute lung injury (ALI), have become a global health problem. The excessive activation and recruitment of immunocytes in the lungs, as well as the release of cytokines, are considered the primary causes of ALI. However, the cellular mechanisms involved in ALI remain unknown. Therefore, new therapeutic strategies need to be developed to control inflammation and prevent the worsening of ALI.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Plant Sciences
Yang Liu, Rui Shao, Tongchuan Suo, Junjie Zhu, Erwei Liu, Yajing Wang, Lin Miao, Xiumei Gao
Summary: In this study, the effect of DZQE on inflammation-related benign prostatic hyperplasia (BPH) was investigated. The results showed that DZQE can significantly inhibit prostate enlargement and decrease the inflammation index. Furthermore, DZQE was found to suppress inflammation-associated BPH by regulating the ERK1/2 signal through its active components, Tan IIA and Ba.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Microbiology
Xinqin Zhong, Yucui Zhao, Lu Huang, Jiarui Liu, Kaiyue Wang, Xiumei Gao, Xin Zhao, Xiaoying Wang
Summary: In this study, it was demonstrated that L. johnsonii protects cardiac function, delays cardiac pathology, and improves gut barrier integrity by remodeling the gut microbiome. The beneficial effects of L. johnsonii on cardiac function post-AMI were abrogated by microbiome dysbiosis caused by antibiotics. The enrichment of L. johnsonii reshapes the gut microbiome by increasing the abundance of Muribaculaceae, Lactobacillus, and decreasing Romboutsia, Clostridia UCG-014, which are correlated with cardiac traits and serum metabolic biomarkers.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Lan Li, Jiasong Cao, Sheng Li, Tianyi Cui, Jingyu Ni, Han Zhang, Yan Zhu, Jingyuan Mao, Xiumei Gao, Adam C. Midgley, Meifeng Zhu, Guanwei Fan
Summary: Tissue-resident cardiac macrophage subsets play important roles in cardiac tissue inflammation and repair after acute myocardial infarction (AMI). CC chemokine receptor 2 (CCR2)-expressing macrophages recruit circulating monocytes to infarcted myocardium and promote inflammation. However, small extracellular vesicles (sEV) derived from CCR2 macrophages, which resemble M2-polarized macrophages, have anti-inflammatory effects and contribute to cardiac repair.
Article
Chemistry, Medicinal
Yuxing Huang, Erwei Liu, Xuhua Huang, Jia Hao, Siyuan Hu, Xiumei Gao
Summary: The study investigated the pharmacokinetic properties of Eucommia ulmoides and Psoralea corylifolia in rats. It was found that the combined administration of the two herbs could affect the exposure of the main components of Eucommia ulmoides extract in rats due to altered intestinal absorption. This research provides guidance for the simultaneous clinical administration of Eucommia ulmoides and Psoralea corylifolia.
CHINESE HERBAL MEDICINES
(2023)
Article
Biochemical Research Methods
Xiaoyan Xu, Meiting Jiang, Xiaohang Li, Yu Wang, Meiyu Liu, Hongda Wang, Yueguang Mi, Boxue Chen, Xiumei Gao, Wenzhi Yang
Summary: We propose the concept of a three-dimensional characteristic chromatogram (3D CC) and use it to authenticate various ginseng products through online comprehensive two-dimensional liquid chromatography (LC x LC) technology. By analyzing the stereo peaks and shade depth in the 3D CC, it is feasible to identify and distinguish different ginseng species and Chinese patent medicines. This provides a practical solution for the accurate identification of easily confused traditional Chinese medicines (TCMs).
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Plant Sciences
Benyu Liu, Shiming Fang, Kun Zhou, Lulu Ma, Yaling Shi, Yuefei Wang, Xiumei Gao
Summary: This study aimed to reveal the hepatotoxicity distinction of coumarin-related compounds from glycosides to aglycones in Fructus Psoraleae (FP) and elucidate their potential mechanism. The results showed that P, IP, FA, and IFA were identified as hepatotoxic compounds, while their glycosides were almost non-hepatotoxic. The conversion from glycosides to aglycones played an essential role in FP-induced hepatotoxicity.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Integrative & Complementary Medicine
Xiumei Gao, Chenyan Wang, Yong Ni, Huiling Zhang
Summary: This study aimed to observe the clinical efficacy of acupuncture along fascia, meridians, and nerves combined with ultrasound-guided nerve blockage in the treatment of postherpetic neuralgia. The results showed that the combination treatment was more effective than ultrasound-guided nerve blockage alone.
JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2023)