Journal
CARBOHYDRATE POLYMERS
Volume 206, Issue -, Pages 811-819Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.11.062
Keywords
Fermented burdock; Bacillus subtilis; Lung cancer; Apoptosis; Polysaccharide structure
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Funding
- Major Projects from the Science and Technology Program of Shandong Province (the Key Technology) [2015ZDJS04002]
- Major State Basic Research Development Program of China (973 Program) [2012CB822102]
- High Technology Research and Development Program of China (863 Program) [2012AA021501]
- Natural Science Foundation of Education Department of Anhui Province [KJ2018ZD025]
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In this study, a polysaccharide, BP, was produced by Bacillus subtilis using burdock oligofructose as a carbon source. The polysaccharide BP was isolated from burdock fermented through B. subtilis by hot water extraction, ethanol precipitation, anion exchange and gel filtration chromatography. The molecular weight of BP was determined to be 1.533 x 10(7) g/mol, and is composed of mannose, rhamnose, glucose, galactose, and arabinose in the ratio of 1:0.12:17.16:0.69:1.10. Furthermore, BP is made up of a backbone structure of (1 -> 6)-linked alpha-D-glucopyranosyl residues substituted at the O-3 position with alpha-D-glucopyranosyl branches. In vitro, BP inhibited A549 cell proliferation and increased reactive oxygen species production, thus, promoting the apoptosis of cells. Moreover, BP up-regulated the expression of caspase-3 and the corresponding protein, indicating that it activates the caspase-3 pathway. These findings suggest that the potential of BP as a supplement or drug to treat cancer.
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