Article
Biochemical Research Methods
Martin Lycka, Vratislav Peska, Martin Demko, Ioannis Spyroglou, Agata Kilar, Jiri Fajkus, Miloslava Fojtova
Summary: This study introduces a new online toolset called WALTER for the rapid, reproducible, and user-friendly analysis of telomere length data and statistical evaluation, representing a major upgrade in TRF scan image processing.
BMC BIOINFORMATICS
(2021)
Article
Entomology
Zhi-Chao Yan, Hai-Qing Hua, Guang-Yuan Qi, Yuan-Xi Li
Summary: We developed a method to detect and identify Trichogramma larvae in their host eggs and successfully differentiated eight Trichogramma species using a PCR-RFLP assay. This study provides a promising approach for conveniently identifying Trichogramma in insectaries and accurately evaluating parasitism rates in the field.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Plant Sciences
Jenna T. B. Ekwealor, Simone D. Benjamin, Jordan Z. Jomsky, Matthew A. Bowker, Lloyd R. Stark, D. Nicholas McLetchie, Brent D. Mishler, Kirsten M. Fisher
Summary: This study presents a technique to determine the genotypic sex of S. caninervis moss and examines the genotypic and phenotypic sex ratios in a case study. The results show that the genotypic ratio of non-expressing shoots is female-biased and exceeds the phenotypic ratio. Additionally, the distribution of male and female genotypes is not predicted by sex expression patterns in different microhabitats.
APPLICATIONS IN PLANT SCIENCES
(2022)
Article
Microbiology
Cheng-Hung Lai, Yu-Sin Lin, Chao-Min Wang, Poa-Chun Chang, Wei-Yau Shia
Summary: This study identified Capnocytophaga strains in the oral cavity of dogs using 16S rRNA and phylogenetic analysis. A novel 16S rRNA PCR-RFLP method was designed to accurately distinguish C. cynodegmi from C. canimorsus, improving detection accuracy. This method is important for the epidemiological study of Capnocytophaga and the rapid diagnosis of human C. canimorsus infections following exposure to small animals.
MICROBIOLOGY SPECTRUM
(2023)
Article
Horticulture
Inmaculada Rodriguez-Torres, Ana Carrillo, Maria del Pilar Ramirez, Francisco Jesus Gomez Galvez, Leonardo Velasco Arjona, Carlos Padilla, Enrico Cretazzo
Summary: Andalusia, a Spanish region known for its diversity, is home to various minority wine varieties. This study conducted genotypic characterization of 98 wine specimens from six areas in Andalusia using thirteen microsatellite markers. The analysis revealed 33 different genotypes, including 12 previously unidentified varieties. The study also determined the eco-geographical groups of the new identified genotypes and tested for the presence of grapevine viruses.
Article
Plant Sciences
Clizia Villano, Silvia Procino, Giuseppe Blaiotta, Domenico Carputo, Nunzio D'Agostino, Ermanno Di Serio, Valentina Fanelli, Pierfederico La Notte, Monica Marilena Miazzi, Cinzia Montemurro, Francesca Taranto, Riccardo Aversano
Summary: Sexual reproduction and spontaneous somatic mutations contribute to the variability and genetic similarity in cultivated grapevine populations. The study identifies genetic loci associated with essential traits, offering opportunities for grapevine breeding initiatives.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Maher Al Rwahnih, Olufemi J. Alabi, Min Sook Hwang, Tongyan Tian, Dimitre Mollov, Deborah Golino
Summary: In 2012, a dormant canes of a proprietary wine grape were added to the collection of the University of California-Davis Foundation Plant Services. Research revealed the presence of a mechanically transmissible virus in the grape, leading to the discovery of a novel bipartite nepovirus-like genome. The new virus was classified within the genus Nepovirus based on its biological and molecular characteristics.
Article
Virology
Martin Jagunic, Angelo De Stradis, Darko Preiner, Pierfederico La Notte, Maher Al Rwahnih, Rodrigo P. P. Almeida, Darko Voncina
Summary: This study investigated the transmission and host range of two newly described grapevine viruses in Croatia. It found that the vine mealybug is a vector of these viruses and can transmit them through vine-to-vine transmission. Grafting and green grafting techniques were also shown to transmit the viruses.
Article
Biochemistry & Molecular Biology
Muhammad Salman Zahid, Muzammil Hussain, Yue Song, Jiajia Li, Dinghan Guo, Xiangyi Li, Shiren Song, Lei Wang, Wenping Xu, Shiping Wang
Summary: Root-zone restriction can improve fruit quality by regulating the diversity and networking of bacterial communities. Plants under restricted cultivation have higher bacterial richness and diversity at different phenological stages. Furthermore, bacterial activities also affect nutrient availability in treated plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wioletta Izabela Wujcicka, Marian Kacerovsky, Adrian Krygier, Michal Krekora, Piotr Kaczmarek, Mariusz Grzesiak
Summary: This study identified three gene polymorphisms-ANGPT2 rs3020221, TLR2 rs3804099, and TLR9 rs187084-that were significantly associated with spontaneous preterm labor (PTL), adjusted for factors influencing pregnancy development. These findings provide important clues for understanding the genetic risk and mechanisms of preterm birth.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Catherine L. A. Salvini, Benoit Darlot, Jack Davison, Mathew P. Martin, Susan J. Tudhope, Shannon Turberville, Akane Kawamura, Martin E. M. Noble, Stephen R. Wedge, James J. Crawford, Michael J. Waring
Summary: This study introduces a method of using DNA-encoded chemical libraries to rapidly discover potent ligands. By coupling active fragments to a partially constructed library, a potent inhibitor with 51 nM affinity was identified in a single step for BRD4. This approach is efficient and suitable for drug discovery.
Article
Veterinary Sciences
Wei Zhao, Jialei Shi, Yongxiu Yao, Hongxia Shao, Aijian Qin, Kun Qian
Summary: This study successfully isolated and molecularly characterized two strains of Chicken astrovirus (CAstV), and observed their effect on hatchability. The genetic analysis showed that these two strains had typical characteristics of avian astroviruses, with high similarity among Chinese strains and a common origin with strains from the UK.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Horticulture
Anca Butiuc-Keul, Ana Coste
Summary: Grapevine cultivation has adapted to changing conditions and demands through advancements in biotechnology and molecular biology, allowing for genetic improvement. These technologies have led to better cultivar identification, trait mapping, and genomic selection, as well as advances in grapevine regeneration, genetic transformation, and genome editing for enhanced yield and quality.
Article
Ecology
Marcos Paolinelli, Georgina Escoriaza, Cecilia Cesari, Sandra Garcia-Lampasona, Rufina Hernandez-Martinez
Summary: Grapevine trunk diseases, particularly hoja de malvon (HDM), pose a significant threat to wine and table grape production in Argentina and Uruguay. The study revealed differences in the microorganisms present in grapevine wood between symptomatic and asymptomatic plants affected by HDM, with a higher presence of certain fungi in plants with more severe symptoms.
Article
Biochemistry & Molecular Biology
Yan Xu, Jiyuan Wang, Ruiqi Wang, Lei Wang, Caixi Zhang, Wenping Xu, Shiping Wang, Songtao Jiu
Summary: The study revealed significant changes in grapevine root architecture and endogenous strigolactone content under root-restriction cultivation, with key roles played by VvCCD8 and VvD27 genes. Treatment with synthetic SL analog GR24 resulted in decreased root length, altered root diameter, and lateral root density.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)