Article
Cell Biology
Masako Fujioka-Kobayashi, Benoit Schaller, Carlos Fernando De Almeida Barros Mourao, Yufeng Zhang, Anton Sculean, Richard J. Miron
Summary: The study characterized the biological properties of Alb-PRF, a new regenerative modality, which demonstrated slow and gradual release of growth factors and high cell biocompatibility, indicating its potential for future clinical applications in medicine.
Article
Orthopedics
Yuta Nakanishi, Takehiko Matsushita, Kanto Nagai, Daisuke Araki, Yuichi Hoshino, Ryosuke Kuroda
Summary: This pilot study compares the release of growth factors from fibrin clot (FC) and leukocyte-rich platelet-rich fibrin (L-PRF) used in knee arthroscopic surgery for meniscal repair. The study found no significant difference in the amount and pattern of growth factor release between FC and L-PRF during the two-week observation period.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Review
Dentistry, Oral Surgery & Medicine
Richard J. Miron, Michael A. Pikos, Nathan E. Estrin, Masako Kobayashi-Fujioka, Alan Rene Espinoza, Hussein Basma, Yufeng Zhang
Summary: This review article summarizes the novel heating process of platelet-rich fibrin (PRF) that extends its working properties and potential applications in various clinical fields. The findings from both preclinical and clinical studies demonstrate the improved stability and degradation properties of the extended-PRF (e-PRF) membrane, as well as its potential use as a replacement for collagen membranes and a regenerative biological filler.
PERIODONTOLOGY 2000
(2023)
Article
Medicine, Research & Experimental
Mohammed Albahrawy, Marwa Abass, Esam Mosbah, Gamal Karrouf, Walaa Awadin, Adel Zaghloul
Summary: This study investigated the regenerative efficacy of leukocyte platelet-rich fibrin (L-PRF) on colon anastomotic healing in rabbits. The results showed that L-PRF can enhance collagen deposition, promote re-epithelialization of the mucosa, stimulate angiogenesis, reduce adhesions, and diminish the degree of stenosis. Therefore, it has potential as a promising aid in healing bowel anastomoses.
Article
Biochemistry & Molecular Biology
Zahra Kargarpour, Jila Nasirzade, Layla Panahipour, Richard J. Miron, Reinhard Gruber
Summary: The study reveals that PRF lysates exhibit potent anti-inflammatory activity in murine mesenchymal cells, reducing cytokine-induced expression of inflammatory mediators and inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mariane Beatriz Sordi, Layla Panahipour, Zahra Kargarpour, Reinhard Gruber
Summary: The study found that PRF can reduce IL-1β release and partially inhibit pyroptosis-related factors in LPS-challenged macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nurul Aida Ngah, George J. Dias, Darryl C. Tong, Siti Noor Fazliah Mohd Noor, Jithendra Ratnayake, Paul R. Cooper, Haizal Mohd Hussaini
Summary: This study successfully prepared lyophilised PRF with a dense and homogeneous 3D fibrin network structure, and demonstrated the release of PDGF-AB. The findings provide a foundation for the potential widespread clinical application of Ly-PRF in craniofacial tissue engineering.
Review
Dentistry, Oral Surgery & Medicine
Richard J. Miron, Masako Fujioka-Kobayashi, Anton Sculean, Yufeng Zhang
Summary: This article summarizes the methods to optimize platelet-rich fibrin (PRF) technology for faster wound healing postsurgery. It explains the differences between RPM and RCF (g-force) and discusses methods to maximize platelet concentrations using optimized protocols. The article also emphasizes the importance of chemically modified PRF tubes without chemical additives and the regulation of temperature for clotting induction/delay. It is the first article to summarize recent literature on PRF for optimizing PRF production for clinical treatment.
PERIODONTOLOGY 2000
(2023)
Article
Biochemistry & Molecular Biology
Indre Jasineviciute, Md Nazmul Hasan, Juozas Grigas, Arnoldas Pautienius, Arunas Stankevicius, Judita Zymantiene, Naoki Miura
Summary: This study aimed to investigate the existence of microRNAs (miRNAs) in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under different storage conditions. Total RNAs were isolated using newly adapted protocols from PRF and other autologous blood products, and quantitative real-time polymerase chain reaction analysis was performed to detect miRNAs. miR-16, miR-21, miR-155, and miR-146a were found to be abundant in PRF and other autologous blood products of canines. Furthermore, these miRNAs were shown to maintain stability under extended freezing temperatures of -30 degrees C for at least one month. Our findings suggest that PRF may serve as a stable resource for various miRNAs in canines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Mirta Hediyati Reksodiputro, Alida Roswita Harahap, Lyana Setiawan, Mikhael Yosia
Summary: PRFM is a new generation of platelet concentrate with a denser and more flexible structure created using a modified method from PRP. This new method offers a cost-effective way to produce ideal PRFM for wound healing. Further studies are needed to compare the performance of PRFMs made with this modified method and PRFMs made with commercial kits.
FRONTIERS IN MEDICINE
(2021)
Article
Ophthalmology
Ning Yang, Siyu Zeng, Juan Yang, Guojing Lu, Lei Du
Summary: This study evaluated the effectiveness of platelet-rich fibrin (PRF) membrane transplantation for the treatment of large idiopathic macular holes (MHs). The results showed that all patients achieved closure of the MHs and had significant improvements in visual acuity and foveal avascular zone. This indicates that PRF transplantation is an effective and safe technique for the treatment of large MHs.
CURRENT EYE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Mohamadreza Tavakoli, Marjan Mirhaj, Saeideh Salehi, Jaleh Varshosaz, Sheyda Labbaf, Atefeh Golshirazi, Nafise Kazemi, Vida Haghighi
Summary: The study demonstrates the fabrication of polyvinyl alcohol (PVA) core and gelatin (Gel) shell core-shell nanofibers containing advanced platelet-rich fibrin (A-PRF). These nanofibers exhibit high porosity, specific surface area, and hydrophilicity. They also possess superior mechanical properties and degradability. The release of growth factors promotes cell proliferation and adhesion, accelerating wound healing. In vivo studies confirm the effectiveness of these nanofiber dressings in full-thickness wound repair.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Masako Fujioka-Kobayashi, Michihide Kono, Hiroki Katagiri, Benoit Schaller, Yufeng Zhang, Anton Sculean, Richard J. Miron
Summary: Platelet-rich fibrin (PRF) was prepared from whole blood using two different centrifugation systems, fixed-angle and horizontal centrifugation. Results showed that the PRF clots produced by horizontal centrifugation had a more uniform blood cell separation compared to those produced by fixed-angle centrifugation. Future research is needed to evaluate the advantage of horizontal centrifugation in clinical practice.
Article
Veterinary Sciences
Anamika Changrani-Rastogi, Krutika Swadi, Mitali Barve, Niyati Bajekal
Summary: This study evaluated the effect of autologous platelet-rich fibrin (PRF) in the management of feline cutaneous wounds. The results showed that PRF could serve as a low-risk and convenient adjunctive therapy to promote wound healing in cats.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Surgery
Pietro Gentile, Simone Garcovich
Summary: This article reviews the literature on the efficacy of autologous activated platelet-rich plasma (PRP) and nonactivated PRP injection for facial rejuvenation. Eleven out of twelve studies showed improved results, despite differences in study design and outcome measures. However, further randomized controlled trials and systematic reviews are needed to confirm the efficacy of PRP injection in facial rejuvenation.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)