Journal
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
Volume 166, Issue 4, Pages 512-516Publisher
SPRINGER
DOI: 10.1007/s10517-019-04384-5
Keywords
impedance spectroscopy; TEER; in vitro models of barrier tissues; intestinal barrier; Caco-2
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Funding
- Russian Science Foundation [16-19-10597]
- Russian Science Foundation [16-19-10597] Funding Source: Russian Science Foundation
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We compared available methods for monitoring the integrity of in vitro models of barrier tissues and studied the possibility of using impedance spectroscopy to solve this problem. It was demonstrated (theoretically and experimentally) that TEER measurements are not sufficiently sensitive to detect small defects in the cell barrier that significantly affect its permeability. For obtaining reliable results, it is necessary to set a sufficiently high threshold TEER, which leads to the loss of many intact samples. At the same time, impedance spectroscopy has all advantages of the classical method of measuring TEER (it is rapid and non-invasive method), while its application in combination with the methods of machine learning allows reliable detection of defects in the cell barrier.
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