Article
Agronomy
Yonglei Zhang, Runnan Yu, Jing Tang, Liheng Du, Yihan Wang, Jiatao Wang, Linsu Liu, Shanshan Gao, Bin Li
Summary: Three insect cytochrome P450 monooxygenase genes (TcCYP4Q3, TcCYP4Q5, and TcCYP4Q7) in Tribolium castaneum are regulated by the CncC signaling pathway and play a role in the susceptibility of the beetles to eugenol, a phytochemical. These findings have implications for the development of novel therapeutics to control pests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Mei Bai, Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Tong-Xian Liu, Hong-Gang Tian
Summary: Our study evaluated the effects of different dsRNA storage conditions on RNAi efficiency. The results showed that the dsRNA molecule was highly stable under typical cryopreservation temperatures and multiple freeze-thaw cycles, and there were no significant differences in RNAi efficiency. Surprisingly, dsRNA remained stable and induced an efficient RNAi response even under refrigerated storage conditions.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Yonglei Zhang, Jiahao Zhang, Dongyu Li, Haidi Sun, Ruixue Lu, Se Yin, Xinlong Guo, Shanshan Gao
Summary: In this study, three TcAOX genes (TcAOX1, TcAOX2, TcAOX3) from Tribolium castaneum genome were identified and characterized. The results showed that TcAOX1 is involved in the degradation of chemical signals, while TcAOX2 and TcAOX3 are associated with plant toxicant detoxification. Knockdown of TcAOX1 led to a reduction in female egg-laying, suggesting its role in fecundity.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shanshan Gao, Ruixue Lu, Yonglei Zhang, Haidi Sun, Siying Li, Kunpeng Zhang, Ruimin Li
Summary: The study found that the OBP gene OBPC12 of Tribolium castaneum may be involved in the insect's defense response to exogenous toxic substances, leading to a decrease in sensitivity to eugenol; The expression pattern of TcOBPC12 in different tissues and developmental stages varies, indicating its potential important role in larvae absorbing exogenous toxic substances.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biology
Marleen Klann, Magdalena Ines Schacht, Matthew Alan Benton, Angelika Stollewerk
Summary: Insects and other arthropods use external sensory structures for mechanosensory, olfactory, and gustatory reception, with sense organs having characteristic shapes related to their functions. While Drosophila melanogaster regulates sense organ identity through specific combinations of transcription factors, other arthropods do not link sense organ subtypes to the same gene expression code. Sense organ diversity may have evolved differently across insect species, with recruitment of sensory genes to distinct sensory organ specification mechanisms playing a role.
Article
Biochemistry & Molecular Biology
Yuan-chen Zhang, Shan-shan Gao, Shuang Xue, Shi-heng An, Kun-peng Zhang
Summary: The study demonstrates that the CYP6BQ7 gene in Tribolium castaneum plays a key role in detoxifying plant toxicants, with its expression induced by Artemisia vulgaris essential oil. Higher expression in larval and adult stages, particularly in the brain and hemolymph, suggests pivotal involvement in detoxification. RNA interference silencing of TcCYP6BQ7 increased larvae mortality in response to essential oil, indicating its importance in detoxification.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Genetics & Heredity
Juanjuan Liu, Shanshan Gao, Luting Wei, Wenfeng Xiong, Yaoyao Lu, Xiaowen Song, Yonglei Zhang, Han Gao, Bin Li
Summary: This study investigates the functions of choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in insect development, reproduction, and insecticide susceptibility. The researchers found that RNA interference of Chat and vacht in Tribolium castaneum resulted in decreased acetylcholine content and disrupted larval development and pupal eclosion. Knockdown of these enzymes also reduced fecundity and egg hatchability. Additionally, the researchers observed that the transcript levels of chat and vacht increased after treatment with carbofuran or dichlorvos, and reduction of chat or vacht decreased the resistance to these insecticides.
Article
Biochemistry & Molecular Biology
Kun Qian, Yi Yang, Changyin Zhou, Haoyu Zhang, Nan Zhang, Xiangkun Meng, Jianjun Wang
Summary: In this study, two putative sodium channel genes, TcNav1 and TcNav2, were cloned and characterized in Tribolium castaneum, encoding proteins with amino acid similarities to Drosophila para. Knockdown of TcNav1 and TcNav2 adversely affected adult emergence and decreased sensitivity to insecticides. These findings suggest that duplication of sodium channel genes in T. castaneum has led to diversified developmental and toxicological functions.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Environmental Sciences
Anna Skourti, Nickolas G. Kavallieratos, Nikos E. Papanikolaou
Summary: This study found that exposure of parental beetles to pirimiphos-methyl affects the reproductive rate and survival of their offspring. As exposure time increases, the reproductive rate of the offspring decreases significantly.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Runnan Yu, Wenjing Zhang, Yanxiao Li, Jing Tang, KumChol Kim, Bin Li
Summary: ALKBH4 plays a crucial role in epigenetic regulation in mammals by repairing alkyl lesions, but its function in insects is not well understood. In this study, the TcALKBH4 gene was cloned and characterized in Tribolium castaneum. The expression pattern of TcALKBH4 showed high expression in early embryos and early pupae, and in the adult testis and ovary. RNA interference targeting TcALKBH4 at different developmental stages resulted in distinct phenotypes, including developmental failure in larvae, reduced food intake, and fertility deficiency in adults. However, TcALKBH4 RNAi did not affect 6mA levels in vivo. qRT-PCR analysis suggested that TcALKBH4 may mediate larval development through the 20E signaling pathway, and the fertility of female and male adults may be regulated by vitellogenesis and JH signaling pathways, respectively. These findings provide new insights into the potential function of ALKBH4 in insects.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Agronomy
In-Young Kim, Byungyoon Choi, Woo-Ram Park, Yu-Ji Kim, Bo-Eun Kim, Seulgi Mun, Hueng-Sik Choi, Don-Kyu Kim
Summary: The study revealed the crucial role of TcHR96 in transcriptional control of P450 enzyme genes for imidacloprid detoxification in T. castaneum. Targeting TcHR96 could potentially help regulate insecticide tolerance in pest beetle populations.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shanshan Gao, Kui Liu, Hui Liu, Se Yin, Xinlong Guo, Yonglei Zhang, Kunpeng Zhang, Ruimin Li
Summary: In this study, a P450 gene (CYP9Z6) was identified from Tribolium castaneum and its role in detoxifying terpinen-4-ol was investigated. The results showed that TcCYP9Z6 plays a significant role in the detoxification of terpinen-4-ol, which suggests its potential as a target for RNAi-mediated prevention and control of T. castaneum.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Janin Rosner, Johanne Tietmeyer, Hans Merzendorfer
Summary: The study focused on the potential functions of TcABCG and TcABCH genes in insecticide detoxification. Upregulation of some genes after insecticide treatment was found, but silencing these genes did not significantly increase susceptibility to insecticides, indicating an unspecific stress response rather than direct involvement in detoxification processes.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Nan Zhang, Jiaping Wei, Heng Jiang, Huichen Ge, Yang Zheng, Xiangkun Meng, Kun Qian, Jianjun Wang
Summary: This study found that arginine kinase (AK) plays an important role in insect response to insecticides by regulating cellular energy balance and participating in detoxification.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jianying Li, Yujie Jia, Dongyue Zhang, Zhen Li, Songdou Zhang, Xiaoxia Liu
Summary: Grapholita molesta, a destructive pest in stone and pome fruits, is primarily controlled by chemical pesticides. However, resistance to insecticides poses a challenge. In this study, the CarE enzyme activity and mRNA expression of six CarE genes were found to increase after treatment with three insecticides. The knockdown of GmCarE9, GmCarE14, GmCarE16, and GmCarE22 resulted in increased susceptibility to the insecticides. This research expands our knowledge of CarEs in insects and their involvement in insecticide susceptibility.
INSECT MOLECULAR BIOLOGY
(2023)