Improved CRISPR-Cas12a-assisted one-pot DNA editing method enables seamless DNA editing

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Advancing biotechnology with CRISPR/Cas9: recent applications and patent landscape

(2018) Raphael Ferreira et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-Mediated Nucleotide Insertion

(2018) Jia Shou et al.   MOLECULAR CELL

Deep learning improves prediction of CRISPR–Cpf1 guide RNA activity

(2018) Hui Kwon Kim et al.   NATURE BIOTECHNOLOGY

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1

(2017) Hiroshi Nishimasu et al.   MOLECULAR CELL

Engineered Cpf1 variants with altered PAM specificities

(2017) Linyi Gao et al.   NATURE BIOTECHNOLOGY

ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes

(2017) Hailong Wang et al.   NUCLEIC ACIDS RESEARCH

A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells

(2017) Mengjun Tu et al.   NUCLEIC ACIDS RESEARCH

CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

(2017) Yu Jiang et al.   Nature Communications

Programmable DNA-Guided Artificial Restriction Enzymes

(2017) Behnam Enghiad et al.   ACS Synthetic Biology

Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition

(2016) Pu Gao et al.   CELL RESEARCH

Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9

(2016) Carolin Anders et al.   MOLECULAR CELL

Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems

(2016) Ryan T. Leenay et al.   MOLECULAR CELL

Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9

(2016) Seiichi Hirano et al.   MOLECULAR CELL

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering

(2016) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1

(2016) Shi-Yuan Li et al.   ACS Synthetic Biology

Multiplexed CRISPR/Cas9- and TAR-Mediated Promoter Engineering of Natural Product Biosynthetic Gene Clusters in Yeast

(2016) Hahk-Soo Kang et al.   ACS Synthetic Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing

(2015) Y. Liu et al.   mBio

Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

(2015) Wenjun Jiang et al.   Nature Communications

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites

(2014) Shengsong Xie et al.   PLoS One

Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination

(2013) Sean D. Colloms et al.   NUCLEIC ACIDS RESEARCH

Fast track assembly of multigene constructs using Golden Gate cloning and the MoClo system

(2012) Stefan Werner et al.   Bioengineered

Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination

(2011) Lin Zhang et al.   Scientific Reports

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

(2009) Carola Engler et al.   PLoS One

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE