Journal
BIOSENSORS & BIOELECTRONICS
Volume 127, Issue -, Pages 167-173Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.12.012
Keywords
CuMn-CeO2-PEI-luminol; Electrochemiluminescence; MNAzyme; Intramolecular co-reaction; Group B Streptococci
Categories
Funding
- Program for Innovation Team of Higher Education in Chongqing [CXTDX201601015]
- National Natural Science Foundation of China [81873980, 21804015]
- Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0206]
- China Postdoctoral Science Foundation [2017M622979]
- Chongqing Postdoctoral Science Special Foundation [XmT2018073]
- Leading Talent Program of CQCSTC [CSTCCXLJRC201719]
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Herein, a novel and pragmatic electrochemiluminescence (ECL) biosensing method was developed for ultra sensitive and specific detection of Group B Streptococci (GBS) by combining self-enhanced luminol complex functionalized CuMn-CeO2 (CuMn-CeO2-PEI-luminol) with MNAzyme-mediated target-recycling amplification. First, the efficient self-enhanced PEI-luminol luminophore was prepared by combining PEI co-reactant with luminol in one molecular, which shortened electron transfer distance and enhanced ECL signal. And CuMn-CeO2 was applied to load a large number of PEI-luminol and strengthen luminous efficiency of luminol by the high catalytic activity toward H2O2 oxidation. Then, target-driven MNAzyme system was used to realize the circulation of GBS nucleic acid sequence, producing plentiful triggers to initiate the hybridization reaction on the surface of electrode. The developed enzyme-free ECL biosensor showed ultra-sensitivity for target DNA detection with detection limits of 68 aM (synthetic DNA) and 5 x 10(2) CFU mL(-1) (genomic DNA extracted from GBS strain). More importantly, this biosensor was successfully applied for detection of genomic DNA of GBS extracted from clinical vaginal/anal swabs as low as 320 copies. Thus, this proposed strategy might be an pragmatic ECL platform for ultrasensitive and specific detection of GBS in clinical vaginal/anal swabs.
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