Journal
BIOSENSORS & BIOELECTRONICS
Volume 127, Issue -, Pages 38-44Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.11.054
Keywords
Photoelectrochemical biosensor; DNA hydroxymethylation; beta-Glucosyltransferase; WS2 nanosheets; Carbon dots
Categories
Funding
- National Natural Science Foundation of China [21775090, 41807484, 21675140, 21375079]
- Natural Science Foundation of Shandong Province, China [ZR2016BM10, ZR2018MB028]
- Dodd Walls Centre for Photonic and Quantum Technologies
- 14th Six Talent Peaks Project in Jiangsu Province [SWYY-085]
- TAP
- PAPD
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5-Hydroxymethylcytosine (5hmC) plays an important role in switching genes on and off in mammals, and it is implicated in both embryonic development and cancer progression. Herein, a novel photoelectrochemical (PEC) biosensor was developed for 5hmC detection based on WS2 nanosheets as the photoactive material and boronic acid functionalized carbon dots (B-CDs) for signal amplification unit. This biosensor can also be used for T4-beta-glucosyltransferase (beta-GT) activity assessment. Firstly, WS2 nanosheets and gold nanoparticles (AuNPs) were immobilized on an ITO electrode surface. Then probe DNA was immobilized on this electrode surface via Au-S bond. Afterwards, the complementary DNA containing 5hmC was then captured on the modified electrode surface by hybridization. Subsequently, beta-GT transferred glucose from uridine diphosphoglucose to the hydroxyl groups of the 5hmC residues. After glycosylation, B-CDs could further be immobilized on the modified electrode surface resulting in a strong photocurrent. The PEC biosensor afforded high selectivity, excellent sensitivity and good reproducibility, with detection limits of 0.0034 nM and 0.028 unit/mL for 5hmC and beta-GT, respectively. Results demonstrate that the photoelectrochemical strategy introduced here based on WS2 nanosheets and B-CDs offers a versatile platform for hydroxymethylated DNA detection, beta-GT activity assessment and beta-GT inhibitor screening.
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