Article
Biophysics
Shereena Joseph, Soumya Rajpal, Debashree Kar, Shital Devinder, Saurabh Pandey, Prashant Mishra, Joby Joseph
Summary: In this study, an optical detection scheme based on guided mode resonance (GMR) was developed for the identification of a specific bacteria. The sensitivity of the sensor was evaluated and a good detection limit was achieved. The developed GMR biosensor is low-cost, easy to handle, and can be transformed into a portable detection modality.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xinke Liu, Ching-Li Tseng, Lu-Yin Lin, Chin-An Lee, Jian Li, Li Feng, Lijun Song, Xiaohua Li, Jr-Hau He, Rajalakshmi Sakthivel, Ren-Jei Chung
Summary: The study developed a LEP immunosensor using Ce3NbO7/CeO2 as a new material, demonstrating high sensitivity, a wide linear range, and a low detection limit. By improving electrode conductivity and surface functionalization, successful LEP immunosensing was achieved, applicable for plasma samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Optics
Tanoy Kumar Dey, Sara Tombelli, Palas Biswas, Ambra Giannetti, Nandini Basumallick, Francesco Baldini, Somnath Bandyopadhyay, Cosimo Trono
Summary: A long period fiber grating was fabricated using sensitivity enhancement techniques, TAP and LP0,2 cladding mode, for label-free IgG/anti-IgG immunoassay. Real-time kinetics of IgG and anti-IgG binding were monitored, achieving a detection limit of 0.16 ng/ml for anti-IgG.
OPTICS AND LASER TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Supakeit Chanarsa, Jaroon Jakmunee, Kontad Ounnunkad
Summary: A bi-functional material based on silver nanoparticles-reduced graphene oxide composite was successfully synthesized for the construction of a label-free electrochemical immunosensor. The material showed good conductivity/electroreactivity and could generate an analytical response for the detection of human immunoglobulin G. Detection of IgG in human serum using the immunosensor displayed satisfactory accuracy and the potential for further development in clinical diagnosis.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Andres F. Cruz-Pacheco, Jennifer Quinchia, Jahir Orozco
Summary: A label-free nanoimmunosensor based on p53/CeO2/PEDOT nanobiocomposite-decorated screen-printed gold electrodes has been developed for the electrochemical detection of anti-p53 autoantibodies. This sensor offers high specificity, selectivity, and long-term storage stability and has the potential to detect anti-p53 autoantibodies in serum samples.
Article
Biochemistry & Molecular Biology
Galina Nifontova, Irina Petrova, Evgeniia Gerasimovich, Valery N. Konopsky, Nizar Ayadi, Cathy Charlier, Fabrice Fleury, Alexander Karaulov, Alyona Sukhanova, Igor Nabiev
Summary: High-throughput protein assays play a crucial role in various fields of biology and medicine. Photonic crystal surface mode(P C SM) imaging is an effective label-free technique for multiplexed analysis of biomolecular interactions. This article describes a microfluidic assay designed for label-free protein biosensing employing PC SM imaging. The results demonstrate the feasibility of simultaneous PC SM imaging of multiple protein interactions, paving the way for further development of this technique.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Peng Xiao, Zhiyuan Xu, Deming Hu, Lili Liang, Lipeng Sun, Jie Li, Yang Ran, Bai-Ou Guan
Summary: Immunosensors are widely used in healthcare, food and drug industry, and environmental protection. This study introduces a temperature-compensated microfiber Bragg grating for immunosensing, which effectively addresses the issue of temperature cross-sensitivity.
ADVANCED FIBER MATERIALS
(2021)
Article
Optics
Maziyar Milanizadeh, SeyedMohammad SeyedinNavadeh, Francesco Zanetto, Vittorio Grimaldi, Christian De Vita, Charalambos Klitis, Marc Sorel, Giorgio Ferrari, David A. B. Miller, Andrea Melloni, Francesco Morichetti
Summary: This paper introduces a novel adaptive multibeam receiver that can separate and simultaneously detect multiple beams sharing the same wavelength and polarization in the optical domain. By using a programmable integrated photonic processor and a two-dimensional array of optical antennas, the separation of beam pairs with different directions and overlapping spatial modes is achieved.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Karel Lacina, Jakub Vzznik, Jakub Sopousek, Zdenek Farka, Veronika Lacinova, Petr Skladal
Summary: Nanoporous surfaces show promise for label-free electrochemical biosensing. This study successfully formed nanopores on electrode surfaces using nonconductive nanoparticles. The permeation characteristics of the redox probe differed based on its concentration, and controlling these parameters is crucial in immunosensing applications.
BIOELECTROCHEMISTRY
(2023)
Article
Physics, Multidisciplinary
Atefe Taheri, Mehrdad Shokooh-Saremi
Summary: A bi-state switch utilizing a two-dimensional, all-dielectric periodic guided-mode resonance structure is proposed in this paper, operating in the visible region with a narrow linewidth reflection resonance. The switch exhibits reversible switching action and shows potential for narrowband optical switches, light modulators, and laser systems.
Review
Chemistry, Multidisciplinary
Juan Wang, Pepijn W. H. Pinkse, Loes Segerink, Jan C. T. Eijkel
Summary: Photonic crystals are materials that can confine and manipulate incident light, and have been extensively studied for the development of optical sensors with high adsorption and enhanced light-matter interaction. They are mainly utilized through five sensing principles, including sensing of refractive index variations, sensing by lattice spacing variations, enhanced fluorescence spectroscopy, surface-enhanced Raman spectroscopy, and configuration transitions.
Review
Chemistry, Multidisciplinary
Andrei V. Kabashin, Vasyl G. Kravets, Alexander N. Grigorenko
Summary: Label-free optical biosensing has great potential in biomedical diagnostics, environmental and food safety, and security. However, it lacks the sensitivity of traditional labelling methods. This article explores ways to overcome the sensitivity barriers and achieve superior sensitivity in label-free biosensing through novel structural architectures, artificial materials, and the use of optical phase as a sensing parameter.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Optics
Mingsen Pan, Zhonghe Liu, Akhil Raj Kumar Kalapala, Yudong Chen, Yuze Sun, Weidong Zhou
Summary: Photonic crystal slabs are at the forefront of research in light confinement, optical wave modulation, and guiding, with abundant real-world applications. A novel 2pi phase control method has been developed for reflected light beams interacting with photonic crystal resonant modes, offering a general guide for the design of phase-shift metamaterials. Enhanced by asymmetric coupling with output ports, this method can be achieved on silicon photonics platforms, suggesting a wide range of applications in sensing, spatial light modulation, and beam steering.
Article
Optics
Yeong Hwan Ko, Nasrin Razmjooei, Hafez Hemmati, Robert Magnusson
Summary: Resonant periodic nanostructures provide perfect reflection through guided-mode resonance mediated by lateral Bloch modes, rather than Mie resonance, highlighting the importance of lattice period on the reflectance properties.
Article
Multidisciplinary Sciences
Krisztian Koos, Gaspar Olah, Tamas Balassa, Norbert Mihut, Marton Rozsa, Attila Ozsvar, Ervin Tasnadi, Pal Barzo, Nora Farago, Laszlo Puskas, Gabor Molnar, Jozsef Molnar, Gabor Tamas, Peter Horvath
Summary: The authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons, which can help increase the number of daily measurements to aid brain research.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Miquel Avella-Oliver, Vicente Ferrando, Juan A. Monsoriu, Rosa Puchades, Angel Maquieira
ANALYTICA CHIMICA ACTA
(2018)
Article
Respiratory System
Javier Milara, Beatriz Ballester, Anselm Morell, Jose L. Ortiz, Juan Escriva, Estrella Fernandez, Francisco Perez-Vizcaino, Angel Cogolludo, Enrique Pastor, Enrique Artigues, Esteban Morcillo, Julio Cortijo
Article
Chemistry, Analytical
Augusto Juste-Dolz, Miguel Avella-Oliver, Rosa Puchades, Angel Maquieira
Article
Chemistry, Analytical
Gabriel Sancho-Fornes, Miquel Avella-Oliver, Javier Carrascosa, Sergi Morais, Rosa Puchades, Angel Maquieira
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Ophthalmology
Alicia Galindo-Ferreiro, Estrella Fernandez, Daniel Weill, Denise C. M. Zornoff, Rajiv Khandekar, Jose Eduardo Corrente, Silvana A. Schellini
SEMINARS IN OPHTHALMOLOGY
(2019)
Article
Ophthalmology
Anna Leszczynska, Blanca Molins, Estrella Fernandez, Alfredo Adan, Santiago Ortiz-Perez
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2019)
Article
Biophysics
Augusto Juste-Dolz, Martina Delgado-Pinar, Miquel Avella-Oliver, Estrella Fernandez, Daniel Pastor, Miguel V. Andres, Angel Maquieira
Summary: The Bio Bragg Gratings (BBGs) present a novel biosensing approach using diffractive structures of protein bioreceptors to transduce the magnitude of biorecognition assays into the intensity of single peaks in the reflection spectrum. Through theoretical and experimental studies, the design, fabrication, and optimization of this system are explored to provide insights into its bioanalytical prospects. The system shows potential for avoiding unwanted signal contributions from non-specific binding and for conceiving multiplexed BBG systems capable of performing multiple label-free biorecognition assays in a single device.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Maria Isabel Lucio, Andy Hernandez Montoto, Estrella Fernandez, Sabri Alamri, Tim Kunze, Maria-Jose Banuls, Angel Maquieira
Summary: A biosensing system based on bioresponsive hydrogels for selective detection of C-Reactive protein (CRP) has been developed, utilizing Direct Laser Interference Patterning to create micro-gratings on the hydrogel substrates. The system achieved detection and quantification limits in human serum dilutions, showing potential for applications related to cardiovascular diseases and sepsis. Further amplification strategies improved sensitivity, widened the linear range, and enhanced limits of detection and quantification.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Plant Sciences
Sergi Munne-Bosch, Sandra Puig, Erola Fenollosa, Andrea Casadesus, Estrella Fernandez
Summary: This study explores the role of vitamin E in protecting seagrass leaves and rhizomes from damage under low temperature conditions. It found that adjustments in antenna size and vitamin E content help protect leaves from sustained photo-inhibitory damage and lipid peroxidation events. Rhizomes also accumulate some vitamin E and are protected from lipid peroxidation during winter. Cold stress can cause transient photo-inhibition in the photosynthetic apparatus of the seagrass.
Article
Nanoscience & Nanotechnology
Augusto Juste-Dolz, Martina Delgado-Pinar, Miquel Avella-Oliver, Estrella Fernandez, Jose Luis Cruz, Miguel Andres, Angel Maquieira
Summary: In this work, a photopatterning method based on UV-laser irradiation is presented to create periodic structures of biomacromolecules by inducing local and mild denaturation of protein biolayers. The resulting nanostructures exhibit a periodic modulation of protein activity, making them suitable for application in diffractive biosensing. The study shows that this UV-based patterning principle is an easy, cost-effective, and fast way to fabricate large areas of homogeneous one-dimensional protein patterns, and these patterns can effectively be used as transducers for biosensing applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Augusto Juste-Dolz, Estrella Fernandez, Rosa Puchades, Miquel Avella-Oliver, Angel Maquieira
Summary: This paper presents a study on the creation of one-dimensional diffractive grooved structures of antigen proteins on glass substrates for label-free detection of antibodies to dairy allergens. By combining microcontact printing with several chemical processes, the protein structures were successfully fabricated and characterized. These structures were further used as optical transducers to sense specific immunoglobulins G, achieving a limit of detection in the range of 30-45 ng/mL of unlabeled antibodies.
Article
Chemistry, Analytical
Paola Zezza, Maria Isabel Lucio, Estrella Fernandez, Angel Maquieira, Maria-Jose Banuls
Summary: The present research aimed to develop a label-free biosensing platform using a biofunctionalized hydrogel with a surface diffractive micropattern. The biosensors were fabricated by holographic recording of polyethylene terephthalate (PET) surface micro-structures and their transfer into a hydrogel material. Two different immobilization approaches, during or after hydrogel synthesis, were demonstrated with the latter showing better loading capacity of the bioreceptor groups. The label-free biosensor showed selective response with a limit of detection of 2.47 μM for the complementary DNA strand, making it a cheap and easy-to-fabricate tool for medical diagnosis.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)