Article
Chemistry, Physical
Md. Ebrahim Khalil, Akanksha Jain, Eeshita Das, Kun Lin Yang, Gobinath Rajagopalan
Summary: Clostridia can produce hydrogen from crude glycerol, but the impurities in glycerol interfere with the process and reduce hydrogen production. By optimizing the process parameters, the efficiency of hydrogen production can be improved, and the process can still demonstrate stability in the presence of high impurity levels.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Agricultural Engineering
Lei Chen, Ke Zhang, Mingpeng Wang, Zhaojie Zhang, Yujie Feng
Summary: The study found that a magnetic field intensity of 3.2 mT effectively enhanced hydrogen production, leading to significantly higher hydrogen yield and production rate. Compared to the control group, the maximum biomass, carbon conversion efficiency, and energy conversion efficiency were also increased to varying degrees.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Anongnart Wannapokin, Hung-Tzu Huang, Pei-Hsuan Chang, Yu-Wen Chien, Chun-Hsiung Hung
Summary: This study developed a novel approach to increase hydrogen yield by co-immobilizing a pure strain of Clostridium pasteurianum CH5 with carboxylic-acid-functionalized multiwalled carbon nanotubes (MWCNT-COOH). After 24-hour preincubation, the hydrogen yield increased by 46.9% compared to the control group and 133.3% compared to the conventional suspended growth method.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Chemistry, Physical
Md. Ebrahim Khalil, Akanksha Jain, Kun Lin Yang, Gobinath Rajagopalan
Summary: The study found that adding rice bran hydrolysate and ferrous chloride to the culture medium of Clostridium beijerinckii G117 can enhance the assimilation of native crude glycerol and hydrogen production. By optimizing the medium conditions, a higher yield of hydrogen and cellular growth was achieved.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Microbiology
Teresa Schoch, Tina Baur, Johanna Kunz, Sophia Stoeferle, Peter Duerre
Summary: 1,3-propanediol (1,3-PDO) is a valuable chemical used in the polymer industry, but its production from petroleum products has environmental disadvantages. This study explores the biobased fermentation of 1,3-PDO from glycerol using recombinant strains of Clostridium beijerinckii. By introducing genes from other strains and optimizing growth conditions, significant increases in 1,3-PDO production were achieved. The use of a specific promoter further enhanced the yield.
Article
Chemistry, Physical
Yang Chen, Yanan Yin, Jianlong Wang
Summary: This study demonstrated that using immobilized microorganisms for hydrogen production from glycerol can enhance both cumulative hydrogen production and hydrogen yield, with improved tolerance to high substrate concentration and higher capability in glycerol utilization. The enhanced hydrogen production may be attributed to the favorable micro-environment created for different microorganisms in immobilized beads.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Energy & Fuels
Sanjeet Mehariya, Antonella Signorini, Antonella Marone, Silvia Rosa
Summary: Simultaneous bioproduction of hydrogen and ethanol from crude glycerol (CG) has the potential for a more cost-effective biofuel generation process. However, the limitations in fermentation processes are related to impurities and lack of nitrogen sources. In this study, a microbial consortium called GlyCeroL (GCL) was used to improve CG fermentation. The results showed increased production of H-2 and EtOH by increasing glycerol concentration, and a nitrogen sparging strategy further improved hydrogen production. The DGGE fingerprint supported the adaptability of GCL to fermentation conditions.
Article
Biotechnology & Applied Microbiology
Sang Hyun Kim, Jeong Hyeon Hwang, Hyun Joong Kim, Suk Jin Oh, Hyun Jin Kim, Nara Shin, Sang-Hyoun Kim, Jeong-Hoon Park, Shashi Kant Bhatia, Yung-Hun Yang
Summary: The study developed Clostridium acetobutylicum as a superior biohydrogen producer by introducing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to enhance hydrogen production. The strain CAC824-G containing gapC showed a 66.3% higher hydrogen production than the wild-type strain, with increased NADH and NADPH pools. Overexpression of gapC resulted in increased hydrogen production even in the presence of inhibitors.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sang Hyun Kim, Hyun Joong Kim, Shashi Kant Bhatia, Ranjit Gurav, Jong-Min Jeon, Jeong-Jun Yoon, Sang-Hyoun Kim, Jungoh Ahn, Yung-Hun Yang
Summary: In this study, a novel hydrogen producing bacterial strain, Clostridium sp. SH25, was screened and found to have higher hydrogen-producing activity on glycerol. The optimal culture conditions were determined, and the strain was successfully applied for practical hydrogen production in a small hydrogen car unit. The discovery of this efficient strain suggests the potential application of biosystems in biohydrogen production.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2022)
Review
Energy & Fuels
Taha Attarbachi, Martin D. Kingsley, Vincenzo Spallina
Summary: The global community's efforts to reduce dependence on fossil fuels have led to an oversupply of crude glycerol due to the increased production of biodiesel. This review paper provides an overview of the global glycerol market, discusses advanced crude glycerol purification technologies, and highlights the challenges and potential solutions for industrial implementation. The results suggest that membrane-based separation technologies driven by pressure, heat, and electrochemistry can effectively purify glycerol, but hybrid solutions may be necessary to address impurities from waste-based feedstocks in biodiesel production.
Review
Biotechnology & Applied Microbiology
Eugenia Leandro Almeida, Jose Eduardo Olivo, Cid Marcos Goncalves Andrade
Summary: Biodiesel, a substitute for petrochemical diesel, can be obtained from renewable raw materials. However, its production leads to a market saturation of glycerol, causing devaluation and economic unfeasibility. Despite this, research explores the use of glycerol as a raw material for biofuel production, driven by environmental concerns.
FERMENTATION-BASEL
(2023)
Review
Chemistry, Physical
Jianlong Wang, Yanan Yin
Summary: Clostridium species play a significant role in dark fermentative hydrogen production, with high hydrogen yield and wide substrate range, although efforts are still needed to ensure efficient and stable operation of the system and economic applicability.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Engineering, Environmental
A. Morales-Marin, J. L. Ayastuy, U. Iriarte-Velasco, M. A. Gutierrez-Ortiz
Summary: The study found that spinel-derived catalysts were more suitable than alumina supported nickel catalysts for biohydrogen production by Aqueous-Phase Reforming of glycerol, with Mg and Ce doping playing a positive role in increasing nickel dispersion and basicity. Furthermore, Mg doping helped hinder nickel reduction, and both promoters improved the stability of the catalyst's textural properties under APR conditions.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Yaeseong Hong, Philipp Arbter, Wei Wang, Lilian N. Rojas, An-Ping Zeng
Summary: The study successfully introduced the glycine synthase system genes from Gottschalkia acidurici into Clostridium pasteurianum, resulting in changes in product metabolism. Activation of glycine synthase led to the secretion of 2-oxobutyrate as a metabolic intermediate of Clostridium pasteurianum for the first time. These findings contribute to a better understanding of the metabolic pathways of Clostridium pasteurianum.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Byungchan Kim, Suk Jin Oh, Jeong Hyeon Hwang, Hyun Jin Kim, Nara Shin, Shashi Kant Bhatia, Jong-Min Jeon, Jeong-Jun Yoon, Jaehung Yoo, Jungoh Ahn, Jung -Ho Park, Yung-Hun Yang
Summary: Petrochemical-based plastics are environmentally detrimental and difficult to degrade, making them a major cause of pollution. As an alternative, pol-yhydroxybutyrate (PHB) has similar properties, but its high production cost poses a significant challenge to industrialization. This study utilized crude glycerol as a carbon source to improve the efficiency of PHB production, selecting Halomonas taeanenisis YLGW01 as the strain of choice due to its salt tolerance and high glycerol consumption rate. Through medium optimization and activated carbon treatment, PHB production was maximized, resulting in 10.5 g/L of PHB with 60% PHB content in fed-batch fermentation. The physical properties of the produced PHB were analyzed, including weight average molecular weight, number average molecular weight, and polydispersity index. The extracted intracellular PHB demonstrated improved flexibility and decreased brittleness compared to authentic film. This study confirms the potential of YLGW01 for industrial PHB production using crude glycerol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Manish Kumar Gupta, Adarsh Kumar Chiranjivi, Tanmay Dutta, Vikash Kumar Dubey, Latha Rangan
Summary: This study reports the synthesis and characterization of a zinc derivatized compound DHDM-Zn for the development of new antileishmanial agents. The study confirmed the interaction between DHDM and zinc through various spectroscopic techniques. DHDM-Zn exhibited excellent antagonistic effects against L. donovani promastigote and had an impact on cell cycle and growth kinetics.
Review
Biochemistry & Molecular Biology
Kumari Prerna, Vikash Kumar Dubey
Summary: This article discusses the interplay between autophagy and apoptosis and their importance in health and diseases, with a focus on Beclin1 as a key player.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Archisha Prakash, Subhomoi Borkotoky, Vikash Kumar Dubey
Summary: Before the rise of SARS-CoV-2, the emergence of other coronaviruses has alerted researchers to the possibility of future novel pathogens. In this study, the researchers focused on identifying inhibitors that target the main protease of coronaviruses, which plays a critical role in their life cycle. Using computational methods, they screened FDA-approved drugs and found three potential inhibitors that can target both the monomeric and dimeric sites of the main protease. These compounds show stable behavior in simulations and have equal binding capacity for both targeted sites, suggesting their potential as inhibitors against SARS-CoV-2 main protease.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Materials Science, Multidisciplinary
Rahul Kumar, Divya, Supratim Mahapatra, Vikash Kumar Dubey, Pranjal Chandra
Summary: This study aimed to engineer N-acetyl-D-glucosamine (NADG) coupled quercetin-loaded nano-lipid-based carriers (NADG-Q-NLBCs) for targeting breast cancer. The constructed nano-bioconjugate was characterized and the in vitro drug release profiles were examined. Results showed that NADG-Q-NLBCs exhibited higher cytotoxicity and induced apoptosis in breast cancer cells in a targeted manner.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Materials Science, Multidisciplinary
Rahul Kumar, Neelima Varshney, Supratim Mahapatra, Sanjeev Kumar Mahto, Vikash Kumar Dubey, Pranjal Chandra
Summary: In this study, lactoferrin-conjugated lipid polymer-based nanoparticles (LLPBNPs) encapsulating methotrexate (MTX) as a potential anticancer drug candidate were synthesized using a simple precipitation method. The physicochemical properties of the hybrid-nano-bioconjugate were characterized, and the encapsulation efficiency and drug release kinetics were analyzed. Additionally, the morphological changes of cancer cells were studied.
MATERIALS TODAY COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Rahul Kumar, Daphika S. Dkhar, Rohini Kumari, Divya, Supratim Mahapatra, Ananya Srivastava, Vikash Kumar Dubey, Pranjal Chandra
Summary: Cancer is a major global health issue, and traditional treatment methods have limitations. Nano lipid-based carriers (NLBCs) have emerged as a promising solution for drug delivery. This review discusses various ligands used to modify NLBCs and provides a list of approved ligand-decorated lipid-based nanomedicines and their clinical status.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Pharmacology & Pharmacy
Rahul Kumar, Daphika S. Dkhar, Rohini Kumari, Supratim Mahapatra, Vikash Kumar Dubey, Pranjal Chandra
Summary: In this study, the types, preparation methods, advantages, and limitations of lipid nanoparticles have been discussed. Several examples of lipid-based nanomedicines that are either approved or under clinical trials were presented. The paper focuses on the latest research in developing lipid-based nanocarriers for various biological applications.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Ivana Cengic, Ines C. Canadas, Nigel P. Minton, Elton P. Hudson
Summary: In this study, a theophylline-responsive riboswitch-induced CRISPR/Cas9 system was developed for precise and markerless genome editing in cyanobacterium. This single-vector system enables multiplexed editing and allows for the generation of markerless mutants.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tanuj Handa, Debanjan Kundu, Vikash Kumar Dubey
Summary: The importance of protein structure in performing biological functions optimally is being challenged by the emergence of intrinsically disordered proteins (IDPs) and intrinsically disordered protein regions (IDPRs). Understanding the evolutionary and functional significance of these proteins is crucial in studying various organisms. This review highlights the importance of IDPs in the physiology of specific organisms and establishes a positive correlation between IDPs and their relevance in human health and future medicine. Extensive research on IDPs and IDPRs will greatly contribute to expanding the field of structural biology, aiding in the comprehension of protein folding, misfolding, associated diseases, and drug design.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Guillaume Pregnon, Nigel P. Minton, Philippe Soucaille
Summary: E. limosum B2, an acetogen capable of producing butyrate and acetate from methanol, was subjected to adaptive laboratory evolution on methanol mineral medium. The evolved populations showed a significant increase in growth rate and exhibited changes in protein and methylome profiles, indicating the involvement of epigenetic regulation in metabolic improvements.
Article
Biochemical Research Methods
Bashir L. Rumah, Benedict H. Claxton Stevens, Jake E. Yeboah, Christopher E. Stead, Emily L. Harding, Nigel P. Minton, Ying Zhang
Summary: In this study, a CRISPR/Cas9 genome editing system was constructed and successfully utilized to delete and insert genes in two types of methanotrophic bacteria, Methylococcus capsulatus Bath and Methylocystis parvus OBBP. The combination of homology-directed repair achieved high frequencies of gene deletions and insertions. Various parameters were also investigated to optimize the CRISPR/Cas9 system in Methylocystis parvus OBBP. This work demonstrated the first efficient CRISPR/Cas9 system generating scarless clean gene deletions and insertions in methanotroph genomes.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Naveena Menpadi, Jay Prakash, Debanjan Kundu, Pranjal Chandra, Vikash Kumar Dubey
Summary: This study investigated the potential inhibitors of DPCK for anti-leishmanial drug development by using computational and experimental approaches. Veratramine, Azulene, Hupehenine, and Hederagenin were identified as promising hit compounds. In-vitro experiments showed that Hupehenine and Veratramine exhibited better inhibition on L. donovani promastigotes compared to Hederagenin and Azulene.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Immunology
Alexander M. Rawson, Andrew W. Dempster, Christopher M. Humphreys, Nigel P. Minton
Summary: Clostridium botulinum is a bacteria that is classified based on its ability to produce botulinum neurotoxin (BoNT). BoNT is the main virulence factor and the cause of botulism, a potentially fatal disease characterized by paralysis. The BoNT is used for medical treatments and in the cosmetic industry due to its potency and specificity.
Article
Microbiology
James Millard, Alexander Agius, Ying Zhang, Philippe Soucaille, Nigel Peter Minton
Summary: Targeted mutations in Eubacterium limosum were achieved using an inducible counter-selective system combined with a non-replicative integrating mutagenesis vector. This system allowed for precise gene deletions, including genes involved in histidine biosynthesis, methanol metabolism, and L-carnitine demethylation. The desired mutants could be obtained with only one induction step after the initial selection of transformant colonies. This approach enables quick and efficient gene editing of E. limosum.
Article
Biotechnology & Applied Microbiology
Barbara Bourgade, James Millard, Christopher M. Humphreys, Nigel P. Minton, M. Ahsanul Islam
Summary: This study demonstrates the significance of a replicating shuttle vector for genetic and metabolic engineering in industrially important M. thermoacetica. The vector was successfully applied for non-native ethanologenesis, allowing ethanol production in this organism.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
