Article
Biochemistry & Molecular Biology
Kazem Mahmoudzadeh, Zohreh Habibi, Maryam Yousefi, Mostafa Mostafavi, Mehdi Mohammadi
Summary: Increasing attention has been given to peptides as potential therapeutics, leading to the search for new peptide synthetic approaches and modifications. Enzymes have recently gained attention as a novel strategy and powerful tool for peptide and protein ligation. This study presents a fully convergent chemoenzymatic peptide synthesis process for the synthesis of aviptadil, a therapeutic peptide, using different peptiligase variants in aqueous environments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Yongsheng Liu, Rezan Gueler, Yunqi Liao, Anzhelika Vorobyeva, Olof Widmark, Theodorus J. J. Meuleman, Anna Koijen, Leendert J. J. van den Bos, Robert Naasz, Vitalina Bodenko, Anna Orlova, Caroline Ekblad, Vladimir Tolmachev, Fredrik Y. Y. Frejd
Summary: This study explores the use of Chemo-Enzymatic Peptide Synthesis (CEPS) to produce a therapeutic HER2 targeted Affibody molecule. The resulting molecule shows thermodynamic stability, binding specificity to HER2, and extended time in circulation. CEPS proves to be a viable method for producing functional Affibody-fusion molecules.
Article
Biotechnology & Applied Microbiology
Sonia Chadha
Summary: Blast disease caused by Magnaporthe oryzae is a significant challenge for rice production worldwide. Chemical fungicides are unsafe and contribute to the emergence of resistant strains. Antimicrobial peptides, such as human salivary peptide histatin 5 (Hst5), offer a safe and effective alternative. This study investigated the antifungal activity of Hst5 on M. oryzae, finding that it causes morphological defects, inhibits germination and formation of blast lesions, and interacts with the fungus' genomic DNA. The multi-target antifungal mechanism of Hst5 provides an environmentally friendly option to combat blast infections in rice and could be explored for other crop pathogens as well.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tian Wang, Chuntong Li, Meijing Wang, Jiachen Zhang, Qingyun Zheng, Lujun Liang, Guochao Chu, Xiaolin Tian, Haiteng Deng, Wei He, Lei Liu, Jinghong Li
Summary: ISG15 protein tools are crucial for studying innate immunity and antiviral responses. This study discovered that the leader protease from foot-and-mouth disease virus can facilitate ligation reactions between ISG15 and synthetic compounds, resulting in the generation of necessary ISG15 protein tools.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Ruifeng Li, Marcel Schmidt, Tong Zhu, Xinyu Yang, Jing Feng, Yu'e Tian, Yinglu Cui, Timo Nuijens, Bian Wu
Summary: The PALME platform is a multi-enzyme platform that allows for sequence-unconstrained synthesis and modification of biomacromolecules. It offers a solution for the bottleneck in peptide conjugation and has significant potential in various applications.
NATIONAL SCIENCE REVIEW
(2022)
Article
Chemistry, Medicinal
Louisa J. Stewart, YoungJin Hong, Isabel R. Holmes, Samantha J. Firth, Yasmin Ahmed, Janet Quinn, Yazmin Santos, Steven L. Cobb, Nicholas S. Jakubovics, Karrera Y. Djoko
Summary: Histatin-5 is a cationic, His-rich, Zn(II)-binding peptide in human saliva that displays antimicrobial activity against fungal and bacterial pathogens. However, it does not kill streptococcal species that normally colonize the human oral cavity and oropharynx under conditions characteristic of human saliva. Instead, it exerts subtle effects on streptococci by modulating Zn(II) availability.
ACS INFECTIOUS DISEASES
(2023)
Article
Chemistry, Medicinal
Joanna X. Campbell, Sean Gao, Keerthi S. Anand, Katherine J. Franz
Summary: This study investigates the role of Zn2+ in regulating the antifungal activity of Hist-5. Increased Zn2+ concentration prevents peptide internalization, reducing cytotoxicity and membrane disruption. The activity of Hist-5 can be rescued by decreasing the availability of Zn2+.
ACS INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Kyung-No Son, Hyun Lee, Dhara Shah, Sushma Kalmodia, Ryan Cree Miller, Marwan Ali, Arun Balasubramaniam, Stephanie M. Cologna, Hyunjoon Kong, Deepak Shukla, Vinay Kumar Aakalu
Summary: The Sigma-2 receptor (S2R) is an important endoplasmic reticular protein, with Histatin-1 (Hst1) being its endogenous ligand. Hst1 plays important roles in epithelial wound healing and cell migration.
Article
Biochemistry & Molecular Biology
Young Eun Hwang, Seonghun Im, Ju Hyun Cho, Wonsik Lee, Byung-Kwan Cho, Bong Hyun Sung, Sun Chang Kim
Summary: Antimicrobial peptides (AMPs) have been studied as potential antimicrobial coating agents, but an efficient and economical method for AMP production is lacking. Researchers successfully synthesized an AMP coating material and demonstrated its equivalent performance to peptides obtained through solid-phase synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Holly E. Morgan, Zoe L. P. Arnott, Tomasz P. Kaminski, W. Bruce Turnbull, Michael E. Webb
Summary: This paper presents a method using evolved sortase variants and depsipeptide substrates for site-specific labeling, which expands the biochemical protein labeling toolkit. An orthogonal pair of enzymes accepting LPEToG and LPESoG depsipeptides has been identified and successfully applied to dual N-terminal labeling.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Pedro Torres, Victor Flores, Tania Flores, Patricio Silva, Luis Gonzalez, Luis A. Cordova, Montserrat Reyes, Vicente A. Torres
Summary: Histatin-1, a salivary peptide, stimulates cell adhesion, migration, and differentiation in bone-lineage cells, contributing to bone regeneration. In a mouse model, histatin-1 increased intra-osseous mineralization, collagen matrix deposition, and closure of cortical defect edges. It also promoted the expression of the osteogenic marker alkaline phosphatase and blood vessel formation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Sang Min Lee, Kyung-No Son, Dhara Shah, Marwan Ali, Arun Balasubramaniam, Deepak Shukla, Vinay Kumar Aakalu
Summary: This study investigated the effects of histatin-1 (Hst1) on LPS-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 (RAW) macrophages. The results demonstrated that Hst1 can inhibit LPS-induced inflammatory mediator production and MAPK signaling pathways in macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Organic
Xiao-Lei Xie, Jing-Ze Qi, Xiao-Cui Wan, Suo-De Zhang, Yan-Ni Zhang, Ge-Min Fang
Summary: We present a robust and practical method for chemical protein synthesis using an o-nitrobenzyl group as a temporary protective group. Through further investigation, we establish a reliable strategy for the quantitative photodeprotection of the o-nitrobenzyl group. This stable protective group is successfully applied in the chemical synthesis of a programmed death ligand 1 fragment, providing a practical approach for hydrazide-based native chemical ligation.
Article
Biochemistry & Molecular Biology
Taru Dube, Uday B. Kompella, Jiban Jyoti Panda
Summary: The successful application of nanomedicine against glioma relies on the fabrication of specific and efficient glioma targeted multifunctional theranostics. In this study, a novel nanoplatform composed of anisotropic gold nanoroses co-loaded with doxorubicin and the near-infrared active/responsive dye indocyanine green was fabricated. The nanoplatform exhibited targeted abilities and optical imaging functions, and showed promising efficacy in combating glioma.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tobias M. Milzarek, Tobias A. M. Gulder
Summary: This article describes the first total synthesis of fungal sorbicillinoids spirosorbicillinols A-C, with a key step being a Diels-Alder cycloaddition reaction. The work provides a method for synthesizing all natural spirosorbicillinols and unnatural diastereomers.
COMMUNICATIONS CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Chitchanok Anutrakunchai, Jan G. M. Bolscher, Bastiaan P. Krom, Sakawrat Kanthawong, Sorujsiri Chareonsudjai, Suwimol Taweechaisupapong
Article
Microbiology
Erika Acosta-Smith, Karina Viveros-Jimenez, Adrian Canizalez-Roman, Magda Reyes-Lopez, Jan G. M. Bolscher, Kamran Nazmi, Hector Flores-Villasenor, Gerardo Alapizco-Castro, Mireya de la Garza, Jesus J. Martinez-Garcia, Jorge Velazquez-Roman, Nidia Leon-Sicairos
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Irene A. van Dijk, Enno C. I. Veerman, Eric A. J. Reits, Jan G. M. Bolscher, Jan Stap
BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Tjitske Sijbrandij, Antoon J. Ligtenberg, Kamran Nazmi, Petra A. M. van den Keijbus, Enno C. I. Veerman, Jan G. M. Bolscher, Floris J. Bikker
Article
Biotechnology & Applied Microbiology
Saharut Wongkaewkhiaw, Suwimol Taweechaisupapong, Chitchanok Anutrakunchai, Kamran Nazmi, Jan G. M. Bolscher, Surasakdi Wongratanacheewin, Sakawrat Kanthawong
Article
Multidisciplinary Sciences
Marie Rossini Carmela T. Lachica, Chitchanok Anutrakunchai, Saengsome Prajaneh, Kamran Nazmi, Jan G. M. Bolscher, Suwimol Taweechaisupapong
Article
Microbiology
Uriel A. Angulo-Zamudio, Jorge E. Vidal, Kamran Nazmi, Jan G. M. Bolscher, Claudia Leon-Sicairos, Brenda S. Antezana, Adrian Canizalez-Roman, Nidia Leon-Sicairos
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Wei Sun, Andi Shi, Dandan Ma, Jan G. M. Bolscher, Kamran Nazmi, Enno C. Veerman, Floris J. Bikker, Haiyan Lin, Gang Wu
Article
Cell Biology
Dandan Ma, Wei Sun, Kamran Nazmi, Enno C. I. Veerman, Floris J. Bikker, Richard T. Jaspers, Jan G. M. Bolscher, Gang Wu
Article
Biotechnology & Applied Microbiology
Wei Sun, Dandan Ma, Jan G. M. Bolscher, Kamran Nazmi, Enno C. Veerman, Floris J. Bikker, Ping Sun, Haiyan Lin, Gang Wu
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Diana A. Ramirez-Sanchez, Izamar G. Arredondo-Beltran, Adrian Canizalez-Roman, Hector Flores-Villasenor, Kamran Nazmi, Jan G. M. Bolscher, Nidia Leon-Sicairos
Summary: This study focused on the effect of bovine lactoferrin (bLF) and LF peptides on cervical and endometrial cancer cell lines, finding that they inhibited cell proliferation and caused cellular morphological changes and damage. bLF induced apoptosis, LFcin17-30 and LFampin265-284 induced both apoptosis and necrosis, while LF chimera induced necrosis. Additionally, bLF and LF chimera showed an additive interaction with cisplatin against uterine cancer cells.
BIOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Pedro Torres, Nadia Hernandez, Carlos Mateluna, Patricio Silva, Montserrat Reyes, Luis Solano, Sebastian Venegas, Alfredo Criollo, Kamran Nazmi, Floris J. Bikker, Jan G. M. Bolscher, Mauricio Garrido, Monica Caceres, Vicente A. Torres
Summary: Histatin-1 is a novel osteogenic factor that promotes bone cell adhesion, migration, and differentiation, as well as mineralization in vitro. It induces the expression of osteogenic genes and triggers nuclear relocalization of beta-catenin, crucial events required for bone tissue regeneration. These effects are seen not only in osteogenically committed cells, but also in primary mesenchymal cells derived from the apical papilla and dental pulp.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Multidisciplinary Sciences
Saharut Wongkaewkhiaw, Suwimol Taweechaisupapong, Sanguansak Thanaviratananich, Jan G. M. Bolscher, Kamran Nazmi, Chitchanok Anutrakunchai, Sorujsiri Chareonsudjai, Sakawrat Kanthawong
Article
Biochemistry & Molecular Biology
A. J. M. Ligtenberg, F. J. Bikker, J. G. M. Bolscher
Summary: Saliva plays a crucial role in maintaining oral health, and the antimicrobial construct LFchimera, inspired by saliva, exhibits high antimicrobial activity against a broad spectrum of microorganisms. Additionally, LFchimera can inhibit biofilm formation, bacterial invasion of cells, and prevent hemolysis, showcasing its versatility and potential in various applications.
BIOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Saharut Wongkaewkhiaw, Sakawrat Kanthawong, Jan G. M. Bolscher, Kamran Nazmi, Suwimol Taweechaisupapong, Bastiaan P. Krom
