Article
Chemistry, Applied
Katherine A. Pitrolino, Reda M. Felfel, Laura Macri Pellizzeri, Jane McLaren, Alexander A. Popov, Virginie Sottile, Colin A. Scotchford, Brigitte E. Scammell, George A. F. Roberts, David M. Grant
Summary: An innovative multi-layered chitosan scaffold was developed for repairing osteochondral defects, with a distinct gradient of pore size and the addition of nanohydroxyapatite for strength. The scaffold exhibited good mechanical performance under loading, supported attachment and proliferation of human mesenchymal stem cells, and facilitated osteogenic and chondrogenic differentiation in specific layers.
CARBOHYDRATE POLYMERS
(2022)
Article
Materials Science, Multidisciplinary
Xiaofeng Chen, Chaoyin Jiang, Ting Wang, Tonghe Zhu, Xiaolin Li, Jinghuan Huang
Summary: This study designed and developed biphasic osteochondral scaffolds with layer-specific differentiation, achieving simultaneous integrated regeneration of articular cartilage and subchondral bone by manipulating the physicochemical properties and biological properties of the materials.
MATERIALS & DESIGN
(2022)
Article
Chemistry, Multidisciplinary
Cairong Li, Wei Zhang, Yangyi Nie, Dongchun Jiang, Jingyi Jia, Wenjing Zhang, Long Li, Zhenyu Yao, Ling Qin, Yuxiao Lai
Summary: This study develops an integrated PCL-based scaffold for repairing osteochondral defects. By in vitro study, the integrated scaffold stimulates proliferation, chondrogenic differentiation, and osteogenic differentiation of human bone mesenchymal stem cells. After implantation into the osteochondral defect in rats, the integrated bilayer scaffold exhibits well repair ability to facilitate simultaneous regeneration of cartilage and subchondral bone, with completely regenerated cartilage tidemarks.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Materials Science, Biomaterials
Abdolvahab Banihashemian, Soheila Zamanlui Benisi, Simzar Hosseinzadeh, Shahrokh Shojaei, Hojatollah Abbaszadeh
Summary: A biphasic scaffold made of chitosan-hyaluronic acid and alginate-nanohydroxyapatite was developed for the treatment of osteoarthritis. The scaffolds exhibited porous structures and hydrophilic chemical groups. Human chondrocytes cells and human adipose-derived mesenchymal stem cells were seeded onto the scaffold and evaluated using various assays. The scaffold showed potential for cartilage and bone regeneration, making it suitable for osteochondral tissue engineering.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Chemistry, Applied
Jian He, Xulin Hu, Jianfei Cao, Yu Zhang, Jianping Xiao, LiJun Peng, Dongliang Chen, Chengdong Xiong, Lifang Zhang
Summary: A chitosan-coated microsphere scaffold was fabricated for bone regeneration, showing excellent biocompatibility and controlled drug release. The incorporation of PLLA, OA-HA, and VH into the scaffold slowed biodegradability while enhancing mechanical and surface properties. CS coating promoted osteoblast adhesion, and the release of OA-HA stimulated osteogenic proliferation.
CARBOHYDRATE POLYMERS
(2021)
Article
Materials Science, Multidisciplinary
Xueyang Zheng, Na Li, Yong Xu, Chuanxin Zhang, Yueping Ouyang, Depeng Meng
Summary: Stem cell-based tissue engineering combined with a bilayer scaffold loaded with bevacizumab shows promising results in promoting stable osteochondral regeneration and preventing vascular invasion. This study highlights a new avenue for osteochondral regeneration by leveraging anti-angiogenesis activity.
MATERIALS & DESIGN
(2022)
Article
Engineering, Biomedical
Weizhou Jiang, Xiuting Xiang, Minkai Song, Jianlin Shen, Zhanjun Shi, Wenhua Huang, Huan Liu
Summary: This study reports a novel all-silk-derived multi-functional biomaterial platform for osteochondral engineering, which can promote migration and pre-differentiation of endogenous cells in the early stage and regulate chondrogenesis and osteogenesis in the later stage. The composite hydrogel scaffold showed great biocompatibility and achieved successful cartilage and subchondral bone regeneration in experiments.
MATERIALS TODAY BIO
(2022)
Article
Biochemistry & Molecular Biology
Niloofar Khandan-Nasab, Elahe Mahdipour, Saeede Askarian, Mahmoud Reza Kalantari, Navid Ramezanian, Reza Kazemi Oskuee
Summary: Recently, there has been a focus on developing skin equivalents to accelerate wound healing and restore skin. This study aimed to design and characterize a novel hydrogel scaffold using alginate, pullulan, and hyaluronic acid polymers to deliver Adipose-derived mesenchymal Stem Cells (ASC) and promote wound healing in an animal model. The synthesized hydrogel was characterized using various techniques, and in vitro studies demonstrated the attachment, viability, and migration of cells in the scaffold. The cell-seeded hydrogel treatment improved wound healing and closure in an animal model.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Composites
Heba Kandil, Basma Ekram, Mona A. M. Abo-Zeid, Bothaina M. Abd M. El-Hady, Amal Amin
Summary: A promising modified composite scaffold for bone tissue engineering was synthesized, which consisted of hydroxyapatite, hyperbranched polyitaconic acid, and chitosan. The modified scaffold showed improved mechanical strength and bioactivity, as well as anticancer properties and enhanced human skin fibroblasts proliferation.
POLYMER COMPOSITES
(2023)
Article
Materials Science, Multidisciplinary
Shuqing Yang, Wenwei Jiang, Xi Hong, Peixian Chen, Wei Li, Guangyu Yao, Dan Zhou
Summary: This study developed a gelatin-based bilayer porous scaffold for subcutaneous osteochondral regeneration, using the antiangiogenic drug curcumin and the proangiogenic drug Danshen. The results showed that the curcumin-loaded gelatin inhibited tube formation, while the Danshen-loaded gelatin promoted tube formation. The bilayer scaffold successfully achieved subcutaneous osteochondral regeneration when recolonized with bone marrow stem cells.
Article
Veterinary Sciences
Elena De Angelis, Roberta Saleri, Paolo Martelli, Lisa Elviri, Annalisa Bianchera, Carlo Bergonzi, Marta Pirola, Roberta Romeo, Melania Andrani, Valeria Cavalli, Virna Conti, Ruggero Bettini, Benedetta Passeri, Francesca Ravanetti, Paolo Borghetti
Summary: The study demonstrates that 3D printed CH scaffolds have good porosity for colonization and synthetic activity of chondrocytes. Enrichment with hyaluronic acid provides a more stable chondrocyte phenotype. The use of 3D CH scaffolds with PL instead of FBS in the medium results in increased gene expression of relevant ECM components.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Martina Tortorici, Ansgar Petersen, Klara Ehrhart, Georg N. Duda, Sara Checa
Summary: Mechanical and biological factors are known to play key roles in osteochondral defect healing, but the underlying principles and how they can be applied in treatment strategies are still largely unknown. A computational approach was used to investigate the impact of scaffold mechanical and architectural properties on osteochondral defect healing, revealing that scaffold material elastic modulus and structural stability are crucial for supporting the repair process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shrikant Sitaram Kirwale, Swati Sharma, Aniruddha Roy
Summary: Current cell grafting techniques primarily rely on seeding cells on pre-formed scaffolds, which do not mimic the tissue's 3D architecture. This study evaluated a strategy based on poly-electrolyte complexation for 3D engraftment of cells in a porous polymeric scaffold. The results demonstrated that the in-situ forming PEC of CH, CS, and HA had good physicochemical properties for cell grafting and supported grafted cells with improved function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Chenyuan Gao, Wenli Dai, Xinyu Wang, Liwen Zhang, Yue Wang, Yiqian Huang, Zuoying Yuan, Xin Zhang, Yingjie Yu, Xiaoping Yang, Qing Cai
Summary: Osteochondral regeneration is challenging due to limited self-healing ability and the complexity of its structure. This study designs a double-layered scaffold with an incorporated Mg2+ gradient and hypoxia-mimicking deferoxamine (DFO). The scaffold promotes dual-lineage regeneration of both cartilage and subchondral bone, with the upper hydrogel layer promoting chondrogenic differentiation and the lower cryogel layer promoting stem cell migration, matrix mineralization, and osteogenesis.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Shadpour Mallakpour, Elham Azadi, Chaudhery Mustansar Hussain
Summary: Pathogen transmission poses a global threat to human health, and vaccines play a crucial role during pandemics like COVID-19. Expediting vaccine production is essential for preventing infectious disease deaths. Utilizing natural polymers as adjuvants or delivery systems shows potential for enhancing vaccine effectiveness and plays a significant role in combating COVID-19.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Chemistry, Multidisciplinary
Mike Jeon, Mackenzie Halbert, Zachary R. Stephen, Miqin Zhang
Summary: Gadolinium-based chelates are commonly used contrast agents for MRI, but their toxicity and potential retention in patients' bodies have raised safety concerns. Iron oxide nanoparticles have emerged as a promising alternative due to their non-toxic and biodegradable nature, but their development as T-1 contrast agents is complex and challenging. Progress is being made in exploring IONPs as safe and improved alternatives, but there are still many hurdles to overcome before they can be used clinically.
ADVANCED MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Guanyou Lin, Richard A. Revia, Miqin Zhang
Summary: Cancer is a genetic disease caused by the accumulation of gene mutations in a cellular subpopulation, and gene therapy has shown potential to be the ultimate solution for treating cancer. Utilizing nanoscale inorganic carriers for delivering nucleic acids has demonstrated promising results in small-animal models, providing effective and safe treatment for oncological diseases.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Engineering, Biomedical
Xiaojie Lin, Ching Ting Tsao, Masayuki Kyomoto, Miqin Zhang
Summary: Osteoarthritis (OA) is a prevalent chronic disease in the elderly population, with current clinical treatments limited to symptom relief. Injectable natural polymer hydrogels are considered highly promising for controlled drug delivery.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Guanyou Lin, Jianxi Huang, Mengyuan Zhang, Shanshan Chen, Miqin Zhang
Summary: This study reports the development of a novel nanoparticle formulation (NP-Chi-xPEI) that can safely and effectively deliver DNA into breast cancer cells for successful transfection. The NP-Chi-xPEI nanoparticle shows high transfection efficiency and low cytotoxicity, making it a potential reliable vehicle for DNA delivery to breast cancer cells.
Article
Materials Science, Multidisciplinary
Peter A. Chiarelli, Richard A. Revia, Zachary R. Stephen, Kui Wang, Forrest M. Kievit, Jordan Sandhu, Meenakshi Upreti, Seokhwan Chung, Richard G. Ellenbogen, Miqin Zhang
Summary: This study introduces a tumor-targeted nanoparticle that enhances the effectiveness of radiation therapy on a selective cellular basis, improving the treatment outcomes for GBM.
Article
Chemistry, Analytical
Zhiyong Wei, Qingxin Mu, Hui Wang, Guanyou Lin, Miqin Zhang
Summary: The assessment of biodegradability of nanomaterials is crucial for understanding interactions with biological systems and determining potential applications. A study on carbon-based biconcave nanodisks (CBBNs) reveals significant degradation in biological systems, making them suitable for biomedical use.
Article
Chemistry, Analytical
Richard A. Revia, Brandon Wagner, Matthew James, Miqin Zhang
Summary: Cells cultured in three-dimensional scaffolds exhibit a phenotype more similar to in vivo cells, and natural polymers are suitable materials for creating such scaffolds for high-throughput drug screening. However, the high viscosity of natural polymer solutions poses a challenge for dispensing. In this study, an automated scaffold dispensing system was developed using pneumatic control and programming software, allowing for the even distribution of high-viscosity polymer solutions.
Article
Nanoscience & Nanotechnology
Seokhwan Chung, Yutaro Sugimoto, Jianxi Huang, Miqin Zhang
Summary: This study demonstrates the use of peptide-functionalized nanoparticles to deliver siRNA to sensitize glioma cells to drugs and achieve efficient gene silencing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Physical
Seokhwan Chung, Chan Mi Lee, Miqin Zhang
Summary: This review highlights recent progress in the development of nanoparticle-based mRNA delivery systems for the treatment of various liver diseases. Specifically, it focuses on the enhanced cellular uptake and endosomal escape achieved through the use of these nanoparticles. The potential applications of this technology in treating liver cancer and hepatotropic infectious diseases are discussed.
NANOSCALE HORIZONS
(2022)
Review
Chemistry, Multidisciplinary
Guanyou Lin, Miqin Zhang
Summary: The potential of theranostic nanoparticles in tumor treatment is widely recognized due to their ability to integrate multifaceted functionalities into a single nanosystem. These nanoparticles typically consist of an inorganic core for imaging and therapeutic functions, bioinert coatings for improved biocompatibility, controlled drug-loading-release modules, and targeting ligands for specific cell uptake. The design and assembly of these functionalities require sophisticated molecular design and precise execution. Ligand chemistry plays a decisive role in translating theoretical designs into functionalized theranostic nanoparticles. This Account reviews the properties and utilities of capping ligands, drug-loading ligands, and targeting ligands in theranostic nanoparticles.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yuanzhang Tang, Yang Zhou, Guanyou Lin, Miqin Zhang
Summary: Human-induced pluripotent stem cells (hiPSCs) cultured in 3D matrices can benefit from improved cell penetration and pluripotency maintenance by modifying the scaffold with hiPSCs conditioned medium (CM). This study demonstrates that CM treatment promotes uniform cell adhesion and distribution, enhances pluripotency markers, and upregulates genes associated with pluripotency. The results highlight the importance of CM treated scaffolds in maintaining the primitive and undifferentiated phenotype of hiPSCs in 3D matrices.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Yang Zhou, Gillian Pereira, Yuanzhang Tang, Matthew James, Miqin Zhang
Summary: Natural polymer-based porous scaffolds with tunable pore size have been developed as three-dimensional tumor models for high-throughput screening of cancer therapeutics. The CHA scaffold-based platform showed potential for mimicking the in vivo malignancy and revealed the influence of pore size on cell growth kinetics, tumor spheroid morphology, gene expression and drug response. The CHA scaffold-based HTS platform demonstrated comparable performance with commercial tissue culture plates and may provide an improved alternative for future cancer study and drug discovery.
Article
Pharmacology & Pharmacy
Jianxi Huang, Guanyou Lin, Taylor Juenke, Seokhwan Chung, Nicholas Lai, Tianxin Zhang, Tianyi Zhang, Miqin Zhang
Summary: This study introduces a novel approach using iron oxide nanoparticles for safe and efficient mRNA delivery to hard-to-transfect cancer cells. The developed nanoparticles showed enhanced cellular internalization of mRNA, easy mRNA release, and minimal cytotoxicity, suggesting great promise for mRNA therapy against hard-to-transfect cancers.
Article
Pharmacology & Pharmacy
Yoshiki Ando, Fei-Chien Chang, Matthew James, Yang Zhou, Miqin Zhang
Summary: In this study, three-dimensional chitosan scaffolds are developed as microcarriers for the prolonged expansion of human neural stem cells (hNSCs) in bioreactors. The chitosan scaffolds exhibit desirable microstructural characteristics and mechanical properties, allowing for hNSC expansion in dynamic conditions for up to three weeks. This microcarrier approach has the potential for mass production of hNSCs in a three-dimensional setting.
Review
Chemistry, Physical
Ariane Erickson, Peter A. Chiarelli, Jianxi Huang, Sheeny Lan Levengood, Miqin Zhang
Summary: Nanofibers synthesized by electrospinning have great clinical potential in cancer research and treatment, serving as a biomimetic tumor microenvironment model, a drug release platform, and a cancer diagnostic tool. This review provides an overview of nanofiber design and fabrication techniques, discussing the impact of nanofiber properties on their functionality, as well as current and foreseeable barriers to their clinical translation in oncology.
NANOSCALE HORIZONS
(2022)
