Article
Oncology
Nayra M. Al-Thani, Stephanie Schaefer-Ramadan, Jovana Aleksic, Yasmin A. Mohamoud, Joel A. Malek
Summary: The study identified multiple protein-protein interactions within the Wnt beta-catenin pathway, including known and novel interactions involving the APC gene. The novel interactions between APC and transcription factors suggest a potential role of APC in regulating the Wnt signaling pathway in the nucleus. These findings could lead to new drug designs for cancer treatment.
CANCER CELL INTERNATIONAL
(2022)
Article
Gastroenterology & Hepatology
John Gasdal Karstensen, Steffen Bulow, Helle Hojen, Anne Marie Jelsig, Niels Jespersen, Klaus Kaae Andersen, Mads Damsgaard Wewer, Johan Burisch, Hans Christian Pommergaard
Summary: This study found that patients with familial adenomatous polyposis (FAP) have a significantly higher risk of developing cancer compared to controls, mainly due to increased risks of colorectal, pancreatic, and duodenal/small-bowel cancers. Additionally, the risk of developing a second primary cancer is also significantly higher for FAP patients.
Article
Multidisciplinary Sciences
Lautaro Baro, Asifa Islam, Hannah M. Brown, Zoe A. Bell, M. Angeles Juanes
Summary: Cell remodeling relies on dynamic rearrangements of cell contacts powered by the actin cytoskeleton. The tumor suppressor APC nucleate actin filaments and localize at cell junctions. The role of APC-driven actin nucleation in cell junction remodeling remains unknown.
Article
Oncology
Wei Huang, Jin Bian, Xiaoping Qian, Lin Shao, Haiyan Li, Lu Zhang, Lin Wang
Summary: This study presented a case of a 20-year-old CRC patient with mutations in both APC and BRCA1 genes, along with a history of colorectal and other cancers in the family. The first ever coexistence of APC and BRCA1 mutations was identified through genetic analysis, shedding light on the inheritance patterns within the family. This case highlights the importance of evaluating detailed family history and conducting multiple-gene panel testing in patients with hereditary cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Peter Georgeson, Tabitha A. Harrison, Bernard J. Pope, Syed H. Zaidi, Conghui Qu, Robert S. Steinfelder, Yi Lin, Jihoon E. Joo, Khalid Mahmood, Mark Clendenning, Romy Walker, Efrat L. Amitay, Sonja Berndt, Hermann Brenner, Peter T. Campbell, Yin Cao, Andrew T. Chan, Jenny Chang-Claude, Kimberly F. Doheny, David A. Drew, Jane C. Figueiredo, Amy J. French, Steven Gallinger, Marios Giannakis, Graham G. Giles, Andrea Gsur, Marc J. Gunter, Michael Hoffmeister, Li Hsu, Wen-Yi Huang, Paul Limburg, JoAnn E. Manson, Victor Moreno, Rami Nassir, Jonathan A. Nowak, Mireia Obon-Santacana, Shuji Ogino, Amanda Phipps, John D. Potter, Robert E. Schoen, Wei Sun, Amanda E. Toland, Quang M. Trinh, Tomotaka Ugai, Finlay A. Macrae, Christophe Rosty, Thomas J. Hudson, Mark A. Jenkins, Stephen N. Thibodeau, Ingrid M. Winship, Ulrike Peters, Daniel D. Buchanan
Summary: By testing a large number of colorectal cancer patients, researchers have identified a tumor mutational signature that can accurately identify carriers of biallelic pathogenic variants in the MUTYH gene, providing important evidence for mutation classification.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Maria Lourdes Garza-Rodriguez, Victor Trevino, Antonio Ali Perez-Maya, Hazyadee Frecia Rodriguez-Gutierrez, Moises Gonzalez-Escamilla, Miguel Angel Elizondo-Riojas, Genaro A. Ramirez-Correa, Oscar Vidal-Gutierrez, Carlos Horacio Burciaga-Flores, Diana Cristina Perez-Ibave
Summary: In this study, a novel pathogenic germline variant in the APC gene was identified using NGS. The variant was confirmed in multiple family members and associated with atypical clinical symptoms. This variant is classified as a PVS1 variant according to ACMG guidelines, providing evidence for early surveillance and suitable treatment in patients with the variant.
Article
Biochemistry & Molecular Biology
Sum-Fu Chiang, Heng-Hsuan Huang, Wen-Sy Tsai, Bertrand Chin-Ming Tan, Chia-Yu Yang, Po-Jung Huang, Ian Yi-Feng Chang, Jiarong Lin, Pei-Shan Lu, En Chin, Yu-Hao Liu, Jau-Song Yu, Jy-Ming Chiang, Hsin-Yuan Hung, Jeng-Fu You, Hsuan Liu
Summary: This study found that patients with mutated APC have better overall and recurrence-free survival in stage III CRC.
BIOMEDICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Daisuke Yamazaki, Osamu Hashizume, Shiho Taniguchi, Yosuke Funato, Hiroaki Miki
Summary: The study on APC gene using a three-dimensional organoid culture of mouse colon epithelia revealed its role in cell proliferation and differentiation regulation, as well as identified novel characteristics related to tumor development and ectopic differentiation. The findings highlight the importance of investigating molecular mechanisms and biological significance of ectopic differentiation in cancer development using colon organoids.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yung-Fu Wu, Chih-Yang Wang, Wan-Chun Tang, Yu-Cheng Lee, Hoang Dang Khoa Ta, Li-Chia Lin, Syu-Ruei Pan, Yi-Chun Ni, Gangga Anuraga, Kuen-Haur Lee
Summary: Colorectal cancer is a heterogeneous disease with genetic and epigenetic changes, and molecular assessment along with biomarker use for prognosis is gaining attention. This study identifies high mRNA levels of 10 upregulated genes in CRC cells and datasets, showing correlation with worse prognosis in CRC patients. CTNNB1 is found to be a key factor in the interaction with upregulated CRC-associated genes, particularly regulating NKD2, FOXQ1, and CEMIP, which when inhibited, can suppress CRC cell growth, providing efficient biomarkers for prognosis and a new molecular interaction network for CRC.
Article
Genetics & Heredity
Martin R. Graf, Shruti Apte, Esteban Terzo, Simran Padhye, Shuhao Shi, Megan K. Cox, Roger B. Clark, Vijay Modur, Vasudeo Badarinarayana
Summary: Familial adenomatous polyposis (FAP) is a colorectal disease caused by mutations in the APC gene, leading to the growth of adenomatous polyps. The novel macrolide, ZKN-0013, has been shown to restore the function of the APC protein and inhibit the beta-catenin/wnt-pathway in human colon carcinoma cells and a mouse model of FAP. Treatment with ZKN-0013 reduced the number of intestinal polyps, adenomas, anemia, and improved survival.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Gastroenterology & Hepatology
Linfang Li, Wenshen Gu, Xingping Wu, Yufeng Ao, Yiling Song, Xiaohui Li, Qiuyao Zeng
Summary: The study demonstrates that FCEA has potential as a favorable diagnostic marker in intestinal diseases, especially in APC, asymptomatic CRC, and 'double-negative' or 'triple-negative' CRC patients.
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2021)
Review
Oncology
Lila Zhu, Xinyu Li, Ying Yuan, Caixia Dong, Mengyuan Yang
Summary: The APC gene, known as a tumor suppressor gene, has two promoters 1A and 1B. Research on APC has mainly focused on its loss-of-function variants causing familial adenomatous polyposis. However, hypermethylation of the APC CpG sequence is also associated with carcinogenesis in various cancers, especially in gastrointestinal tumors.
FRONTIERS IN ONCOLOGY
(2021)
Review
Pharmacology & Pharmacy
Michal Caspi, Amnon Wittenstein, Michal Kazelnik, Yarden Shor-Nareznoy, Rina Rosin-Arbesfeld
Summary: The Wnt pathway plays a crucial role in regulating embryogenic developmental processes and tissue homeostasis via beta-catenin. Dysregulation of this pathway has been implicated in carcinogenesis, particularly in colorectal cancer. While the Wnt cascade is an attractive target for therapeutic intervention against CRC, its complexity and challenges need to be addressed for effective treatment.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Oncology
Julie Leclerc, Marie Beaumont, Roseline Vibert, Stephane Pinson, Catherine Vermaut, Cathy Flament, Tonio Lovecchio, Lucie Delattre, Christophe Demay, Florence Coulet, Erell Guillerm, Nadim Hamzaoui, Patrick R. Benusiglio, Afane Brahimi, Francois Cornelis, Helene Delhomelle, Sandra Fert-Ferrer, Benjamin P. J. Fournier, Alain Hovnanian, Clementine Legrand, Alain Lortholary, David Malka, Florence Petit, Jean-Christophe Saurin, Sophie Lejeune, Chrystelle Colas, Marie-Pierre Buisine
Summary: AXIN2 variants are associated with colorectal cancer susceptibility, and testing for AXIN2 mutations is clinically relevant for patients with colorectal adenomatous polyposis or cancer. This study identified 10 pathogenic/likely pathogenic AXIN2 variants, with a frequency of 0.24% in patients. AXIN2 variants were strongly associated with colorectal adenomatous polyposis and other digestive cancers.
GENES CHROMOSOMES & CANCER
(2023)
Review
Cell Biology
Chenchen Li, Emma E. Furth, Anil K. Rustgi, Peter S. Klein
Summary: The Wnt signaling pathway is crucial in metazoan development and stem cell maintenance, and is also involved in various malignancies. Apart from the canonical activation, the pathway can affect cell function through multiple alternative effectors, potentially contributing to cancer development.
Article
Oncology
Fereshteh Asgharzadeh, Niloufar Naghibzadeh, Milad Hashemzehi, Asma Mostafapour, Seyed Mahdi Hassanian, Amir Avan, Majid Khazaei
Summary: This study investigates the anti-metastatic effects of valsartan, an angiotensin II receptor type 1 (AT1R) antagonist, in a lung metastasis model of colorectal carcinoma (CRC). The results show that valsartan, especially in combination with 5-fluorouracil (5-FU), significantly reduces lung metastatic nodules and metastatic area. Additionally, it decreases inflammatory and angiogenic markers, while increasing oxidative stress markers. This suggests that valsartan, when used in combination with standard chemotherapeutic agents, can have a synergistic effect in treating CRC lung metastasis.
JOURNAL OF GASTROINTESTINAL CANCER
(2023)
Article
Public, Environmental & Occupational Health
Samira Karbasi, Afsane Bahrami, Mohammad Yahya Hanafi-Bojd, Zahra Khorasanchi, Asghar Zarban, Gordon A. Ferns
Summary: This study aimed to investigate the association between adherence to the DASH dietary pattern and breast milk nutrient content. The results showed that high adherence to the DASH diet was associated with higher levels of DPPH and calcium, and lower levels of MDA and triglyceride in breast milk. Adhering to a healthy dietary pattern can improve the quality of breast milk in lactating women.
MATERNAL AND CHILD HEALTH JOURNAL
(2023)
Article
Integrative & Complementary Medicine
Afsane Bahrami, Amir Masoud Jafari-Nozad, Samira Karbasi, Malaksima Ayadilord, Gordon A. Ferns
Summary: The objective of this study was to evaluate the efficacy of curcumin supplementation on cognitive abilities in women with premenstrual syndrome (PMS) and dysmenorrhea. A randomized, triple-blind, placebo-controlled trial was conducted, involving 124 women with both PMS and dysmenorrhea. The results showed that curcumin supplementation significantly improved memory, inhibitory control, and selective attention in these women. Curcumin was found to be safe and well-tolerated in the trial. This study suggests that curcumin has a beneficial effect on cognitive function in women with PMS and dysmenorrhea.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Mahdi Vahedi, Ali Shahini, Mehran Mottahedi, Setareh Garousi, Seyed Ali Shariat Razavi, Ghazaleh Pouyamanesh, Amir R. Afshari, Gordon A. Ferns, Afsane Bahrami
Summary: This study investigates the anti-tumor activity of harmaline on glioblastoma cells. The results demonstrate that harmaline inhibits cell proliferation, induces cell cycle arrest and apoptosis. Additionally, harmaline suppresses metastasis and migration of the cells.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Nutrition & Dietetics
Samira Karbasi, Malihe Mohamadian, Mohsen Naseri, Zahra Khorasanchi, Asghar Zarban, Afsane Bahrami, Gordon A. A. Ferns
Summary: This study found an association between maternal adherence to a Mediterranean diet and the total antioxidant capacity of breast milk and infant urine. Individuals with the highest Mediterranean diet scores had significantly higher levels of DPPH in breast milk and infant urine, as well as higher levels of protein and FRAP in breast milk, compared to those with the lowest scores. Furthermore, mothers with the highest diet scores had significantly lower levels of TG in breast milk.
Article
Obstetrics & Gynecology
Samira Karbasi, Malihe Mohamadian, Mohsen Naseri, Mohammad Yahya Hanafi-Bojd, Zahra Khorasanchi, Negar Morovatdar, Asghar Zarban, Afsane Bahrami, Gordon A. Ferns
Summary: This study aimed to evaluate the association between food quality score (FQS) in lactating mothers and the quality indicators of breast milk (BM) and antioxidant content of infant urine. The results showed that high FQS in lactating mothers was associated with a higher quality of BM and higher antioxidant content in infant urine.
BMC PREGNANCY AND CHILDBIRTH
(2023)
Review
Physiology
Atieh Ostadi, Morteza Arab-Zozani, Elham Zarei, Gordon A. Ferns, Afsane Bahrami
Summary: Radiodermatitis (RD) is a common complication in cancer patients undergoing radiation therapy, and there is no effective treatment available. This systematic review aimed to evaluate the efficacy of curcumin supplementation in reducing RD severity. Seven studies were included, and four of them showed that curcumin supplementation had a beneficial effect on RD intensity. These findings suggest the potential clinical use of curcumin in supportive cancer care, but further research is needed to determine the optimal extract, form, and dose of curcumin for RD prevention and treatment.
PHYSIOLOGICAL REPORTS
(2023)
Article
Neurosciences
Samira Karbasi, Zahra Asadi, Zabihullah Mohaghegh, Farhad Saeedi, Gordon A. Ferns, Afsane Bahrami
Summary: There is a correlation between dietary patterns and insomnia in young women, with a higher adherence to Western dietary patterns increasing the odds of insomnia.
NEUROPSYCHOPHARMACOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Afrooz Arzehgar, Fatemeh Davarinia, Gordon A. Ferns, Ali Hakimi, Afsane Bahrami
Summary: This study aimed to investigate the relationship between biomarkers and cognitive ability using machine learning methods. Through questionnaire and biomarker measurements, it was found that serum levels of NOx, alkaline phosphatase, phosphate, and blood platelet count were associated with cognitive abilities in young women.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2023)
Article
Physiology
Amir Talebpour, Mahtab Mohammadifard, Reza Zare Feyzabadi, Sara Mahmoudzadeh, Hadis Rezapour, Mansoore Saharkhiz, Mahboube Tajik, Gordon A. Ferns, Afsane Bahrami
Summary: This study evaluated the effects of curcumin on inflammatory biomarkers and iron profile in young women with premenstrual syndrome (PMS) and dysmenorrhea. The results showed that curcumin significantly decreased serum levels of hsCRP and had no effect on iron metabolism. Therefore, curcumin supplementation may have positive effects on inflammation in women with PMS and dysmenorrhea.
PHYSIOLOGICAL REPORTS
(2023)
Article
Environmental Sciences
Samira Karbasi, Maryam Moradi Binabaj, Zahra Khorasanchi, Milad Bideh, Asghar Zarban, Afsane Bahrami
Summary: This study aimed to assess the impact of a low carbohydrate dietary pattern on the characteristics of breast milk and oxidative stress markers in infant urine. The results showed that adherence to a low carbohydrate diet was associated with improved breast milk quality and markers of oxidative stress in infant urine.
JOURNAL OF HEALTH POPULATION AND NUTRITION
(2023)
Article
Genetics & Heredity
Niloofar Ghobadi, Ghazaleh Khalili-Tanha, Mehrane Mehramiz, Mina Maftooh, Nastaran Bani, Soodabeh ShahidSales, Majid Khazaei, Saeideh Ahmadi-Simab, Raheleh Rahbarian, Hassan Ramshini, Gordon A. Ferns, Masoumeh Gharib, Amir Avan
Summary: This study explored the association between the 1236C > T; rs1128503 genetic variant of the ABCB1/ MDR1 gene and the development and prognosis of esophageal squamous cell carcinoma (ESCC). The results showed that ESCC patients had a higher frequency of the C allele, and individuals with the TT genotype had a higher risk of ESCC. However, this genetic variant was not associated with progression-free survival and overall survival in ESCC patients.
Article
Integrative & Complementary Medicine
Mohammad-Mostafa Askarnia-Faal, Sayyed-Hadi Sayyed-Hosseinian, Seyedeh Elnaz Nazari, Fereshteh Asgharzadeh, Ehsan Vahedi, Moein Eskandari, Haniyeh Ghasemi, Amir Avan, Maryam Alaei, Hamideh Naimi, Maryam Daghiani, Atena Soleimani, Abbas Alalikhan, Reza Mohammadzadeh, Gordon Ferns, Mikhail Ryzhikov, Majid Khazaei, Seyed Mahdi Hassanian
Summary: This study explores the therapeutic potential of curcumin in attenuating post-operative adhesion band formation in animal models. Results showed that curcumin significantly decreased the severity, length, density, and tolerance of peritendinous adhesions as well as the incidence and severity of abdominal fibrotic bands post-surgery. Curcumin may decrease inflammation by regulating the recruitment of inflammatory cells and oxidant/antioxidant balance, and reduce fibrosis by decreasing quantity, quality, and grading of fibers, and collagen deposition.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Chemistry, Medicinal
Sepideh Ebrahimi, Zohreh Mostafavi-Pour, Majid Khazaei, Seyedeh Elnaz Nazari, Shirin Taraz Jamshidi, Mohammad Soukhtanloo
Summary: This study aimed to evaluate the inhibitory role of auraptene in colorectal cancer metastasis, and the results showed that auraptene can inhibit the proliferation and migration of CT26 cells and reduce the metastasis rate by modulating the expression of MMP-2, MMP-9, E-cadherin, VEGF-A, and VEGFR1 genes. It also induced significant necrosis in tumor cells.
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2023)
Review
Dentistry, Oral Surgery & Medicine
Saeedeh Askarian, Masoumeh Gholami, Ghazaleh Khalili-Tanha, Negin Chaeichi Tehrani, Mona Joudi, Majid Khazaei, Gordon A. Ferns, Seyed Mahdi Hassanian, Amir Avan, Marjan Joodi
Summary: Cleft lip and cleft palate are common congenital malformations, with both genetic and environmental factors playing a role in their development.
ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG
(2023)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
