Article
Biochemistry & Molecular Biology
Valeria Guzman-Luna, Andrea G. Quezada, A. Jessica Diaz-Salazar, Nallely Cabrera, Ruy Perez-Montfort, Miguel Costas
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2017)
Article
Biochemistry & Molecular Biology
Andrea G. Quezada, A. Jessica Diaz-Salazar, Nallely Cabrera, Ruy Perez-Montfort, Angel Pineiro, Miguel Costas
Article
Multidisciplinary Sciences
Breyan Ross, Stephan Krapp, Martin Augustin, Reiner Kierfersauer, Marcelino Arciniega, Ruth Geiss-Friedlander, Robert Huber
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Biochemistry & Molecular Biology
Andrea G. Quezada, Nallely Cabrera, Angel Pineiro, A. Jessica Diaz-Salazar, Selma Diaz-Mazariegos, Sergio Romero-Romero, Ruy Perez-Montfort, Miguel Costas
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Ezequiel-Alejandro Madrigal-Carrillo, Carlos-Alejandro Diaz-Tufinio, Hugo-Anibal Santamaria-Suarez, Marcelino Arciniega, Alfredo Torres-Larios
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Claudia Alvarez-Carreno, Gerardo Coello, Marcelino Arciniega
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2020)
Article
Biophysics
Maria Hoyer, Alvaro H. Crevenna, Jose Rafael Cabral Correia, Andrea G. Quezada, Don C. Lamb
Summary: This study used zero-mode waveguides to directly observe the dynamic nucleation process of actin filaments mediated by gelsolin. The stability of actin dimers determines whether elongation occurs and a conformational change in actin associated with filament formation can prevent elongation.
BIOPHYSICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Guilherme G. Moreira, Francois-Xavier Cantrelle, Andrea Quezada, Filipa S. Carvalho, Joana S. Cristovao, Urmi Sengupta, Nicha Puangmalai, Ana P. Carapeto, Mario S. Rodrigues, Isabel Cardoso, Guenter Fritz, Federico Herrera, Rakez Kayed, Isabelle Landrieu, Claudio M. Gomes
Summary: The study demonstrates that S100B protein acts as an extracellular chaperone for Tau, inhibiting its aggregation and providing evidence for neuro-protective functions.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Fernanda Murtinheira, Mafalda Migueis, Ricardo Letra-Vilela, Mickael Diallo, Andrea Quezada, Claudia A. Valente, Abel Oliva, Carmen Rodriguez, Vanesa Martin, Federico Herrera
Summary: Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is a neurodegenerative disorder characterized by progressive cerebellar ataxia, spasticity, motor sensory neuropathy and axonal demyelination. This study reveals that sacsin, the protein associated with ARSACS, is also highly expressed in astrocytes and plays a potential role in glial pathology. The findings suggest a possible connection between ARSACS and intermediate filament-associated diseases and highlight the importance of glia in ARSACS pathology.
Article
Biochemistry & Molecular Biology
Maurizio Cavani, Walter Arnaldo Riofrio, Marcelino Arciniega
Summary: This article investigates the influence of different mutations on the interaction between the SARS-CoV-2 virus and the hACE-2 receptor. Using molecular dynamics and the MM-PBSA method, the study finds that certain mutations have varying effects on the binding affinity, with some mutations compensating each other and one mutation promoting a more complex effect. These findings provide new insights into the specific contributions of concerned mutations.
Review
Medicine, General & Internal
Armando Hernandez-Garcia, Melissa D. Morales-Moreno, Erick G. Valdes-Galindo, Eric P. Jimenez-Nieto, Andrea Quezada
Summary: The emergence of COVID-19 has spurred the development of new diagnostic methods for SARS-CoV-2. Utilizing CRISPR-Cas systems, these methods show promise in achieving similar sensitivity and specificity to the benchmark RT-qPCR, while overcoming its limitations. This review evaluates publicly available CRISPR-Cas-based methods for SARS-CoV-2 detection and provides suggestions for optimizing experimental conditions and parameters.
Article
Neurosciences
Miguel Benitez-Angeles, Ana E. Lopez Romero, Itzel Llorente, Ileana Hernandez-Araiza, Ariela Vergara-Jaque, Fernando H. Real, Oscar Eduardo Gutierrez Castaneda, Marcelino Arciniega, Luis E. Morales-Buenrostro, Francisco Torres-Quiroz, Refugio Garcia-Villegas, Luis B. Tovar-y-Romo, Wolfgang B. Liedtke, Leon D. Islas, Tamara Rosenbaum
Summary: In this study, it was found that the TRPV4 channel is activated by lysophosphatidic acid (LPA), making it one of the few known endogenous agonists for TRPV4. Mechanistic details of the activation of TRPV4 by LPA and lysophosphatidylcholine (LPC) were also provided, demonstrating that LPA interacts with both the N- and C-terminal regions of TRPV4 to activate this channel. Furthermore, it was shown that LPC activates TRPV4 by producing an open state with a different conductance than LPA.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemistry & Molecular Biology
Michal Mikitiuk, Jan Barczynski, Przemyslaw Bielski, Marcelino Arciniega, Urszula Tyrcha, Aleksandra Hec, Andrea D. Lipinska, Michal Rychlowski, Tad A. Holak, Tomasz Sitar
Summary: Lysosome-targeting chimeras (LYTACs) have been developed to facilitate the degradation of specific molecular targets. In this study, pure protein LYTACs based on non-glycosylated IGF2 peptides were developed, which can be easily produced in facilities capable of monoclonal antibody production. These chimeras utilize the IGF2R/CI-M6PR pathway for lysosomal shuttling and effectively target programmed death ligand 1 (PD-L1) with superior results compared to anti-PD-L1 antibodies alone in vitro assays.