Review
Pharmacology & Pharmacy
Vernon LaLone, Doug Smith, Jennifer Diaz-Espinosa, Gus R. Rosania
Summary: Raman confocal microscopes are used to visualize the distribution of small molecule drugs within cells, providing insights into their transport phenomena. Chemical imaging techniques reveal the formation of stable complexes or precipitates of lipophilic drugs inside cells. Raman chemical imaging methods allow measurement and mapping of drug molecules at a microscopic, subcellular level.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Haonan Lin, Hyeon Jeong Lee, Nathan Tague, Jean-Baptiste Lugagne, Cheng Zong, Fengyuan Deng, Jonghyeon Shin, Lei Tian, Wilson Wong, Mary J. Dunlop, Ji-Xin Cheng
Summary: The study presents a system for acquiring high-resolution SRS spectra using a polygon scanner, while significantly improving signal-to-noise ratio through the use of a spatial-spectral residual learning network. The authors demonstrate high-speed imaging and tracking of multiple biomolecules in the fingerprint region using a polygon-based ultrafast delay scanner and deep learning framework.
NATURE COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Dong Joon Lee, Eun Seo Kim, Hyo Won Lee, Hwan Myung Kim
Summary: This mini-review summarizes the recent advances in two-photon fluorescent trackers for monitoring lipid droplets (LDs). The article covers the chemical structures, two-photon properties, live sample imaging, and biomedical applications of representative two-photon fluorescent trackers for LDs. The current challenges and future research trends for two-photon fluorescent trackers of LDs are also discussed.
FRONTIERS IN CHEMISTRY
(2022)
Article
Cell Biology
Triana Amen, Daniel Kaganovich
Summary: Metabolic regulation is essential for stress response pathways, and the interconnection between stress granule formation and lipid droplet accumulation has been uncovered, revealing a novel relationship between stress adaptation mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Optics
Shuo Zhang, Yexuan He, Shuhua Yue
Summary: Cancer cells dysregulate lipid metabolism to support their rapid growth, and coherent Raman scattering (CRS) microscopy provides a powerful tool to study lipid metabolism at the single cell level without labeling. The latest applications of CRS microscopy in cancer research have shed new insights into the role of lipid metabolism in cancer development and progression.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yang Luo, Alberto Martin-Jimenez, Michele Pisarra, Fernando Martin, Manish Garg, Klaus Kern
Summary: The motion of atoms is crucial for chemical and structural transformations. This study demonstrates the detection of vibrational coherences induced by broadband laser pulses on a single graphene nanoribbon using femtosecond coherent anti-Stokes Raman spectroscopy. This suggests a potential breakthrough in tracking and controlling vibrational coherences at the atomic and molecular scales.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Chi Zhang, Stephen A. Boppart
Summary: The study utilized label-free chemical imaging modalities to investigate mitochondrial activities in living cancer cells, revealing that hypothermia exposure may induce fatty-acid accumulation in mitochondria and lead to metabolic abnormalities.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Ying Xu, Xiaoli Hou, Qiaoqiao Zhu, Shijie Mao, Jie Ren, Jidong Lin, Ning Xu
Summary: A noninvasive, label-free, single-cell Raman imaging analysis platform was developed to distinguish between different cell phenotypes based on their intrinsic biochemical profiles; significant differences were found between HeLa cell WT and CDK6 gene knockout types through Raman spectral analysis; insights into the biological mechanisms of cell phenotypes were revealed by studying interactions between organelles.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Robert D. Healey, Essa M. Saied, Xiaojing Cong, Gergely Karsai, Ludovic Gabellier, Julie Saint-Paul, Elise Del Nero, Sylvain Jeannot, Marion Drapeau, Simon Fontanel, Damien Maurel, Shibom Basu, Cedric Leyrat, Jerome Golebiowski, Guillaume Bossis, Cherine Bechara, Thorsten Hornemann, Christoph Arenz, Sebastien Granier
Summary: This study presents the discovery and mechanism of action of the first drug-like inhibitors of alkaline ceramidase 3 (ACER3), as well as the characterization of enzyme:inhibitor interactions using mass spectrometry and MD simulations. The use of novel fluorescent ceramide substrates enables screening of large compound libraries, offering a new paradigm for inhibition of lipid metabolizing enzymes with non-lipidic small molecules. The findings lay the groundwork for targeting ACER3 in drug discovery efforts.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Ali Muhieddine, Natalie Fournier, Hani Dakroub, Ali Assi, Jean-Louis Paul, Ali Tfayli, Pierre Chaminade, Sana Tfaili
Summary: Atherosclerosis, the leading cause of morbidity and mortality in industrialized countries, is associated with the presence of cholesterol-laden foam cells in atherosclerotic plaques. It has been found that EPA inhibits cholesterol esters hydrolysis in macrophages, potentially impairing cholesterol efflux from plaques and hindering the antiatherogenic process.
Article
Optics
David R. Smith, Siddarth Shivkumar, Jeff Field, Jesse W. Wilson, Herve Rigneault, Randy A. Bartels
Summary: Impulsive stimulated Raman scattering (ISRS) is a powerful technique for studying low-frequency Raman vibrational modes, and this study introduces a simple strategy for ISRS spectroscopy and hyperspectral imaging using spectral filters.
Article
Biochemical Research Methods
Richard C. Prince, Eric O. Potma
Summary: Coherent Raman scattering (CRS) microscopy is an optical imaging technique with great potential for biomedical research. Despite significant advancements in specificity, sensitivity, and practicality, broader dissemination of the technique remains a challenge. New developments and technical improvements have expanded the application space of CRS microscopy, but efforts are still needed to attract more users beyond enthusiasts.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Review
Pharmacology & Pharmacy
Junjie Zeng, Wenying Zhao, Shuhua Yue
Summary: The high attrition rates of anti-cancer drugs during clinical development pose a challenge in pharmaceutical industry. Fluorescence microscopy, while commonly used in oncology research, may have limitations due to the large size of fluorescent labels. Coherent Raman scattering microscopy offers a promising solution, providing chemically selective, sensitive, high-resolution imaging without the need for labeling, thus facilitating oncology pharmacokinetic research.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jiajun Du, Haomin Wang, Lu Wei
Summary: As an emerging optical imaging modality, stimulated Raman scattering (SRS) microscopy provides valuable opportunities for chemical biology studies due to its rich chemical information. The development of Raman probes using the chemical biology toolbox has played a crucial role in advancing SRS microscopy and expanding biological applications. This perspective discusses the development of biorthogonal SRS imaging using small tagging and isotopes, highlighting their unique advantages for metabolic pathway analysis and microbiology investigations. It also proposes potential opportunities for chemical biology studies integrating small tagging with SRS imaging. Additionally, it summarizes the current designs of highly sensitive and super-multiplexed SRS probes and provides future directions and considerations for next-generation functional probe design, aiming to bridge the gap between SRS microscopy and chemical biology research.
ACS CHEMICAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Menghuan Tang, Kai Lin, Mythili Ramachandran, Longmeng Li, Hongye Zou, Huzhi Zheng, Zhao Ma, Yuanpei Li
Summary: This study developed lipid-small molecule hybrid nanoparticles for imaging and treatment of glioma. These hybrid nanoparticles have low toxicity, enhanced photodynamic therapy effects, and the ability to visualize drug distribution and tumor imaging.
ACTA PHARMACEUTICA SINICA B
(2022)