Journal
ANALYTICA CHIMICA ACTA
Volume 1048, Issue -, Pages 143-153Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2018.10.014
Keywords
Fluorescent probe; Hypochlorous acid; Near-infrared; Si-rhodamine; Lysosome
Categories
Funding
- National Natural Science Foundation of China [21505032, 21722501, 21605038, 21605039, 21702051, 21405034]
- China Postdoctoral Science Foundation [2017M612405]
- key scientific research project of higher education of the Henan province [15A150016, 15A150013, 16A150013, 14B150020]
- Henan Normal University [qd14126]
- Youth Science Foundation of Henan Normal University [2014QK13]
- Program for Science Technology Innovation Talents in Universities of Henan Province [18HASTIT001]
- Program for Innovative Research Team in University of Henan Province [17IRTSTHN001]
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Hypochloric acid (HClO) is mainly distributed in acidic lysosomes of phagocytes and closely associated with numerous physiological and pathological processes, especially inflammatory response. Fluorescent probe has become an important tool for imaging HClO in lysosomes, but suffered from interference from autofluorescence in vivo, phototoxicity to biosamples and photobleaching phenomenon due to their short-wavelength excitation and emission. Unfortunately, up to now, no near-infrared (NIR) lysosome-targetable fluorescent probe has been reported for imaging HClO. In this paper, a near-infrared fluorescent probe Lyso-NIR-HClO for imaging lysosomal HClO was reported for the first time. Lyso-NIR-HClO based on Si-rhodamine is consisted of a morpholine unit as a lysosome-targetable group and a HClO-mediated cyclization reaction site as a response group, which was applied for highly selective and sensitive detection and imaging for endogenous and exogenous HClO in lysosomes, with a linear range from 5.0 x 10(-8) to 1.0 x 10(-5) M and a detection limit of 2.0 x 10(-8) M in vitro. Attributed to NIR emission and excellent photostability of Si-rhodamine, Lyso-NIR-HClO exhibits excellent performances in vivo, such as low interference from intracellular autofluorescence, stable and persistent fluorescence signal and good tissue penetration, which are in favor of accurate, time-lapse and long-term imaging for HClO. Finally, we applied the probe Lyso-NIR-HClO to visualize endogenous HClO during lysosome-involved inflammatory response including bacteria-infected cells and inflamed mouse model with satisfactory results. The above results proved that Lyso-NIR-HClO would be a potentially useful tool for the study of biological functions and pathological roles of HClO in lysosomes, especially role of lysosome in the inflammatory response. (C) 2018 Elsevier B.V. All rights reserved.
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