Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 81, Issue 5, Pages -Publisher
WILEY
DOI: 10.1111/aji.13104
Keywords
decidual NK cells; decidualization; Gal-1; PIBF; progesterone receptor isoforms
Categories
Funding
- Europen Union [EFOP-3.6.3-VEKOP-16-2017-00009, GINOP-2.3.2-15-201600021, EFOP-3.6.1.-16-2016-00004]
- University of Rijeka, Croatia [13.06.1.1.08]
- Croatian science foundation [HRZZ 3432]
- Pecs University [AOK-KA 2017-22]
Ask authors/readers for more resources
Problem The progesterone-regulated genes, PIBF and Gal-1, are key players in the feto-maternal immunological interaction. This study aims to investigate the expression of PIBF and Gal-1 in WT and progesterone receptor KO models as well as subsequent effects of PIBF on decidualization of stromal cells. Method of the study PRAKO, PRBKO and PRKO BALB/c mice were used for assessing the role of PR isoforms in PIBF induction. PIBF- and Gal-1 mRNA expression in the uterus was tested by real-time PCR. The effect of PIBF on decidualization of endometrial stromal cells was verified by anti-desmin immunofluorescence. Immunohistochemistry was used for testing PIBF expression in the uterus. Gal-1, ER alpha and PR positive decidual NK cells were detected by immunofluorescence. Results PIBF mRNA was significantly increased in progesterone-treated WT mice, but not in PRKO and PRAKO mice. PIBF protein expression was reduced in the endometria of PRKO and PRAKO, but not in PRBKO mice. During a 6-day culture, PIBF induced decidual transformation of endometrial stromal cells. PIBF expression in the mouse uterus was highest during the implantation window, while Gal-1 mRNA expression continuously increased between day 2.5 and day 11.5 of gestation. Decidual NK cells express Gal-1 and ER alpha, but not PR at day 7.5 murine pregnancy. Conclusion PIBF produced via engagement of PRA, is highly expressed in the endometrium during the implantation window, and plays a role in decidualization. The concerted action of PIBF and Gal-1 might contribute to the low cytotoxic activity of decidual NK cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available