Article
Multidisciplinary Sciences
Wenjie Yu, Thomas O. Moninger, Andrew L. Thurman, Yuliang Xie, Akansha Jain, Keyan Zarei, Linda S. Powers, Alejandro A. Pezzulo, David A. Stoltz, Michael J. Welsh
Summary: Submucosal glands (SMGs) play a crucial role in protecting the lungs but can also contribute to disease. In cystic fibrosis (CF), SMGs produce abnormal mucus that disrupts mucociliary transport. This study used single-cell RNA sequencing, immunohistochemistry, and in situ hybridization to investigate the expression and distribution of ion transporters and cell types in SMGs. The results suggest that loss of epithelial anion secretion, rather than intrinsic cell defects, causes CF mucus abnormalities. The study also revealed different functions of acinar mucous cells and acinar serous cells in producing mucins and antimicrobials, respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Qian Hao, Jiajia Li, Qinghua Zhang, Fei Xu, Bangxiang Xie, Hua Lu, Xiaohua Wu, Xiang Zhou
Summary: This study using single-cell transcriptomics analysis of HGSOC samples reveals new molecular features and potential therapeutic targets, which could advance the understanding and treatment of the disease.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Otorhinolaryngology
Onyekachi Ezinna Nnabue, Steven D. Pletcher, Jose G. Gurrola, Andrew N. Goldberg, Kristiana M. Jordan, Sarah M. Knox, Alison J. May
Summary: This study identifies significant alterations in the structure and function of submucosal glands (SMGs) in the posterior septum of adult chronic rhinosinusitis (CRS) patients, suggesting reduced contribution to mucociliary clearance (MCC). These findings may provide targets for future therapeutic interventions for both nasal and sinus diseases.
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2021)
Article
Anatomy & Morphology
Chiman Kumari, Richa Gupta, Mayank Sharma, Justin Jacob, Ravi K. K. Narayan, Daisy Sahni, Ashutosh Kumar
Summary: This study examined the anatomical and functional characteristics of the nasopharyngeal tubal glands, and found that they are taxonomically different from the salivary glands. The presence of respiratory epithelium and the absence of salivary amylase support their classification as part of the respiratory tract rather than salivary glands.
JOURNAL OF ANATOMY
(2023)
Article
Biochemistry & Molecular Biology
Eun-Ah Christine Song, Monika Che, Jason Osinski, Kirsten Smalley, Erich Horeth, Satrajit Sinha, Rose-Anne Romano
Summary: ANp63 is crucial in maintaining and renewing myoepithelial cells in the salivary glands. These cells function as bipotent progenitor cells during homeostasis and contribute to the differentiation of ductal and acinar cells. However, their contribution to regeneration following severe injury is independent of delta Np63.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Allergy
Anabel Gonzalez-Gil, T. August Li, Ryan N. Porell, Steve M. Fernandes, Haley E. Tarbox, Hyun Sil Lee, Kazuhiro Aoki, Michael Tiemeyer, Jean Kim, Ronald L. Schnaar
Summary: The study identified a high molecular weight Siglec-8 ligand on the mucus layer of human airways, composed of DMBT1S8 with picomolar affinity for Siglec-8. This finding is important for understanding the pathogenesis and treatment of airway diseases.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Yun Mao, Sonika Patial, Yogesh Saini
Summary: This study investigated the role of airway epithelium-specific HMGB1 in the pathogenesis of muco-obstructive lung disease in Tg+ mice. The deficiency of HMGB1 in airway epithelial cells in Tg+ mice resulted in increased inflammation and impaired bacterial clearance.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Nam Soo Joo, Hyung-Ju Cho, Meagan Shinbashi, Jae Young Choi, Carlos E. Milla, John F. Engelhardt, Jeffrey J. Wine
Summary: The combination of a low dose of the cholinergic agonist and forskolin or a beta adrenergic agonist synergistically increased mucus clearance velocity, showing potential as a therapeutic approach for muco-obstructive diseases such as cystic fibrosis.
SCIENTIFIC REPORTS
(2021)
Review
Respiratory System
Camille Ehre, Gunnar C. Hansson, David J. Thornton, Lynda S. Ostedgaard
Summary: Cystic fibrosis (CF) is a genetic disorder caused by dysfunction of the CFTR protein, leading to mucus accumulation in the lungs, intestines, and pancreatic ducts. This review article discusses recent progress in understanding CFTR expression in different airway regions and cells, as well as the production and clearance of mucus from the lungs. Animal models are also explored for insights into the organization of the mucin network and the protective role of mucus and antimicrobial activities against invading pathogens.
JOURNAL OF CYSTIC FIBROSIS
(2023)
Review
Pharmacology & Pharmacy
Rosy Ghanem, Veronique Laurent, Philippe Roquefort, Tanguy Haute, Sophie Ramel, Tony Le Gall, Thierry Aubry, Tristan Montier
Summary: The respiratory epithelium is susceptible to diseases like cystic fibrosis, which impact airway function. Artificial mucus models play a crucial role in evaluating gene transfer systems' effectiveness in reaching the epithelium.
Article
Cell Biology
Wenjie Yu, Thomas O. Moninger, Michael V. Rector, David A. Stoltz, Michael J. Welsh
Summary: This study reveals the sensory capacity of pulmonary neuroendocrine cells (PNECs) in sensing and responding to external stimuli. The researchers discovered PNECs located within submucosal glands that can sense an inflammatory molecule called succinate, and they found that succinate triggers ATP release from PNECs, causing gland contraction and promoting mucus ejection.
DEVELOPMENTAL CELL
(2022)
Review
Genetics & Heredity
Konstantin Kochergin-Nikitsky, Lyubava Belova, Alexander Lavrov, Svetlana Smirnikhina
Summary: Gene therapy for genetically determined diseases requires efficient transgene delivery methods, with rAAV vectors being widely used due to their simplicity and ability to transduce eukaryotic cells. Various approaches, such as capsid modifications and tissue-specific promoters, have been explored to retarget rAAV to respiratory cells. Challenges remain in transduction of stem and progenitor lung cells using AAV.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Chemistry, Medicinal
Tosifa A. Memon, Lili Sun, Marysol Almestica-Roberts, Cassandra E. Deering-Rice, Philip J. Moos, Christopher A. Reilly
Summary: This study investigated the protective effect of Vasaka tea, a medicinal plant commonly used to treat respiratory malaise, on airway epithelial cells exposed to wood smoke particles. The results showed that Vasaka tea can inhibit the production of excessive mucus and protect the cells from damage and death caused by wood smoke particles. The study provides mechanistic insights and supports the potential use of Vasaka tea as a preventative and/or restorative therapy for lung inflammatory conditions.
Article
Immunology
Marko Manevski, Dinesh Devadoss, Christopher Long, Shashi P. Singh, Mohd Wasim Nasser, Glen M. Borchert, Madhavan N. Nair, Irfan Rahman, Mohan Sopori, Hitendra S. Chand
Summary: Research has found that long noncoding RNA (lncRNA) plays an important role in cigarette smoke exposure and chronic obstructive pulmonary disease. Blocking the expression of lncRNA can alleviate inflammation and excessive mucus production caused by cigarette smoke.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Meng Jiang He, Xin Yang Liu, Jia Xin Xu, Xiao Yue Xu, Quan Lin Li, Wei Feng Chen, Ping Hong Zhou
Summary: This study retrospectively analyzed 15 patients with gastric adenocarcinoma of the fundic gland type (GA-FG) and evaluated the efficacy and safety of endoscopic submucosal dissection (ESD) for GA-FGs. The results showed that ESD is effective and safe for the treatment of early-stage GA-FGs.
JOURNAL OF DIGESTIVE DISEASES
(2022)