Article
Plant Sciences
Tetsuya Ishida, Yasuhiro Tanaka, Takanori Maruta, Takahiro Ishikawa
Summary: Ascorbate plays a crucial role in plants and its biosynthesis is regulated by the D-Man/L-Gal pathway in the photosynthetic tissues, with the VTC2 gene as a key regulator. The liverwort Marchantia polymorpha exhibits distinctive mechanisms for ascorbate biosynthesis and regulation, unlike other land plants.
Article
Plant Sciences
Mario Fenech, Vitor Amorim-Silva, Alicia Esteban del Valle, Dominique Arnaud, Noemi Ruiz-Lopez, Araceli G. Castillo, Nicholas Smirnoff, Miguel A. Botella
Summary: The study analyzed the subcellular localization and potential interactions of enzymes involved in L-ascorbate biosynthesis pathway in photosynthetic organisms. It was found that most enzymes showed dual cytosolic/nuclear localization and could interact with each other through coimmunoprecipitation, although direct interactions were not observed in yeast-two hybrid analysis. The research highlighted the crucial role of GDP-L-galactose phosphorylase (GGP) in regulating ascorbate synthesis and its impact on enzyme interactions within the pathway.
Article
Microbiology
Wei Mao, Noureddine Lazar, Herman van Tilbeurgh, Philippe M. Loiseau, Sebastien Pomel
Summary: Leishmaniasis is a vector-borne disease with limited treatment options. GDP-mannose pyrophosphorylase (GDP-MP) has been identified as a potential therapeutic target for the development of new antileishmanial drugs. In this study, the enzymatic properties of recombinant L. infantum GDP-MP (LiGDP-MP) were analyzed, revealing a mutation at position 258 compared to the homolog in L. donovani. Further studies are needed to determine the significance of this residue change for L. infantum.
Article
Agronomy
Kanyanat Lamanchai, Deborah L. Salmon, Nicholas Smirnoff, Pornsawan Sutthinon, Sittiruk Roytrakul, Kantinan Leetanasaksakul, Suthathip Kittisenachai, Chatchawan Jantasuriyarat
Summary: Ascorbic acid (AsA) or Vitamin C plays a significant role in biological processes in plants. The OsVTC1-1 gene in rice is involved in AsA synthesis and cell wall-related processes. OsVTC1-1 RNAi lines showed reduced transcript levels, AsA content, leaf anatomical parameters, and changes in cell wall composition and associated proteins.
Article
Biology
Yuqi Yi, Lulu Liu, Wenyan Zhou, Daiyin Peng, Rongchun Han, Nianjun Yu
Summary: In this study, a guanosine diphosphate (GDP)-mannose pyrophosphorylase (DhGMPP) involved in the biosynthesis of polysaccharides in Dendrobium huoshanense was identified and characterized. Understanding the function of DhGMPP could provide insights into enhancing polysaccharide accumulation through metabolic engineering in D. huoshanense.
OPEN LIFE SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Cong Fu, Xuexia Xu, Yukang Xie, Yufei Liu, Min Liu, Ai Chen, Jenny M. Blamey, Jiping Shi, Suwen Zhao, Junsong Sun
Summary: In this study, a novel microbial engineering platform for the fermentation production of l-fucose was established by introducing heterologous GDP-l-fucose synthesis pathway and engineering GDP-mannose mannosyl hydrolase (WcaH) in Bacillus subtilis. By rational design and mutagenesis, the efficiency of GDP-l-fucose hydrolysis by WcaH was enhanced. The engineered strain with WcaH(R36Y/N38R) produced 1.6 g/L l-fucose during shake-flask growth, which was 67.3% higher than wild-type WcaH. The accumulated l-fucose concentration in a 5 L bioreactor reached 6.4 g/L.
MICROBIAL CELL FACTORIES
(2023)
Article
Plant Sciences
Kanyanat Lamanchai, Nicholas Smirnoff, Deborah L. Salmon, Athipat Ngernmuen, Sittiruk Roytrakul, Kantinan Leetanasaksakul, Suthathip Kittisenachai, Chatchawan Jantasuriyarat
Summary: This study investigates the role of the OsVTC1-1 gene in rice blast fungus inoculation and finds that the gene regulates rice blast resistance through multiple defense mechanisms, including hormone synthesis and signaling pathways.
Article
Plant Sciences
Xiaoying Liu, Rongmei Wu, Sean M. Bulley, Caihong Zhong, Dawei Li
Summary: This study identifies two new transcriptional activators, AceMYBS1 and AceGBF3, that positively regulate GGP gene and increase AsA accumulation. AceMYBS1 activates AceGGP3 expression and overexpression of AceMYBS1 effectively increases AsA accumulation. AceGBF3 interacts with AceMYBS1 and together they promote AceGGP3 expression. Additionally, ABA represses AceMYBS1, leading to decreased AceGGP3 expression and AsA accumulation.
Article
Plant Sciences
Tsubasa Sodeyama, Hitoshi Nishikawa, Kenji Harai, Daiki Takeshima, Yoshihiro Sawa, Takanori Maruta, Takahiro Ishikawa
Summary: Ascorbate is an important redox compound in plants, primarily synthesized through the d-mannose/l-galactose pathway in streptophytes. Physcomitrium patens was found to possibly possess both the d-mannose/l-galactose pathway and the d-galacturonate pathway. Moss ALase was characterized as a functional enzyme involved in ascorbate metabolism, with PpALase1 playing a significant role in dehydroascorbate degradation rather than ascorbate biosynthesis.
Article
Multidisciplinary Sciences
Yan-Hua Liu, Hao Wang, Jie-Xia Liu, Sheng Shu, Guo-Fei Tan, Meng-Yao Li, Ao-Qi Duan, Hui Liu, Ai-Sheng Xiong
Summary: This study cloned the AgGMP gene from celery and investigated its function in ascorbic acid accumulation and response to drought stress. The results showed that AgGMP gene was up-regulated under drought stress in celery, and transgenic Arabidopsis plants carrying the AgGMP gene had higher ascorbic acid content and longer root lengths.
Article
Biochemistry & Molecular Biology
Helene Levaique, Olivier Pamlard, Cecile Apel, Jerome Bignon, Margaux Arriola, Robin Kuhner, Khalijah Awang, Philippe M. Loiseau, Marc Litaudon, Sebastien Pomel
Summary: Leishmaniasis is a vector-borne disease caused by the protozoan parasite Leishmania found in tropical and sub-tropical areas, affecting 12 million people worldwide. Limited treatments for this disease have toxicity and resistance issues. A study identified a compound active against GDP-MPs, prompting the need for further optimization to improve its antileishmanial activity.
Article
Chemistry, Physical
Peng Jin, Yuanyuan Wang, Zhengang Liang, Miao Yuan, Hua Li, Qizhen Du
Summary: This study successfully demonstrated the enzymatic bioconversion of d-fructose to d-mannose using a recombinant thermostable N-acyl-d-glucosamine 2-epimerase, resulting in high yields of d-mannose within 1 hour. The newly developed biocatalyst system shows potential for large-scale industrial production of d-mannose.
CATALYSIS SCIENCE & TECHNOLOGY
(2021)
Article
Plant Sciences
Paul Deslous, Celine Bournonville, Guillaume Decros, Yoshihiro Okabe, Jean-Philippe Mauxion, Joana Jorly, Stephanie Gadin, Cecile Bres, Kentaro Mori, Carine Ferrand, Sylvain Prigent, Tohru Ariizumi, Hiroshi Ezura, Michel Hernould, Christophe Rothan, Pierre Petriacq, Yves Gibon, Pierre Baldet
Summary: Novel regulators underlying high ascorbate content in tomato fruits were identified using forward genetics and mapping-by-sequencing approaches. Mutants with increased ascorbate levels were characterized, revealing a cis-acting upstream open reading frame (uORF) mutation affecting downstream regulation of GDP-L-galactose phosphorylase (GGP). The high ascorbate content was found to impact floral organ architecture, leading to male sterility in tomato plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Jamison P. Huddleston, Thomas K. Anderson, Nicholas M. Girardi, James B. Thoden, Zane Taylor, Hazel M. Holden, Frank M. Raushel
Summary: Campylobacter jejuni is coated with a capsular polysaccharide (CPS) on its exterior cell surface, whose monosaccharide components' identity and sequences are strain-dependent. Proteins such as Cj1430 play crucial roles in catalyzing important reactions for bacterial survival.
Article
Biotechnology & Applied Microbiology
Mengke Xue, Sizhong Feng, Fang Xie, Hongyang Zhao, Yemin Xue
Summary: In this study, the first hyperthermophilic L-arabinose/D-galactose 1-dehydrogenase (TmAraDH) from Thermotoga maritima was heterologously purified from Escherichia coli. It belongs to the Gfo/Idh/MocA protein family, prefers NAD(+)/NADP(+) as a cofactor. The purified TmAraDH exhibited high catalytic efficiency and stability, suggesting its potential application in the conversion of L-arabinose and D-galactose.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Marianela Cobos, Sheyla Perez, Janeth Braga, Gabriel Vargas-Arana, Leenin Flores, Jae D. Paredes, J. Dylan Maddox, Jorge L. Marapara, Juan C. Castro
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Review
Cardiac & Cardiovascular Systems
Jose Luis Zamorano, Christer Gottfridsson, Riccardo Asteggiano, Dan Atar, Lina Badimon, Jeroen J. Bax, Daniela Cardinale, Antonella Cardone, Elizabeth A. M. Feijen, Peter Ferdinandy, Teresa Lopez-Fernandez, Chris P. Gale, John H. Maduro, Javid Moslehi, Torbjorn Omland, Juan Carlos Plana Gomez, Jessica Scott, Thomas M. Suter, Giorgio Minotti
EUROPEAN JOURNAL OF HEART FAILURE
(2020)
Article
Genetics & Heredity
Marianela Cobos, Gad E. Grandez, J. Dylan Maddox, Carlos G. Castro, Hicler N. Rodriguez, Segundo L. Estela, Miguel A. Grandez, Jae D. Paredes, Rodil Tello-Espinoza, Pedro M. Adrianzen, Jorge L. Marapara, Juan C. Castro
Summary: The study sequenced, de novo assembled, and functionally annotated the complete mitochondrial genome of the native oleaginous microalgae Ankistrodesmus falcatus strain UCP001 from the Peruvian Amazon basin. The mitogenome is a circular double-stranded DNA molecule of 41,048 bp with 49 genes, enriching genomic resources for further research. Phylogenetic analysis showed its close relation to other microalgae species.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Forestry
Muhammad Azhar Nadeem, Stalin Juan Vasquez Guizado, Muhammad Qasim Shahid, Muhammad Amjad Nawaz, Ephrem Habyarimana, Sezai Ercisli, Fawad Ali, Tolga Karakoy, Muhammad Aasim, Rustu Hatipoglu, Juan Carlos Castro Gomez, Jorge Luis Marapara del Aguila, Pedro Marcelino Adrianzen Julca, Esperanza Torres Canales, Seung Hwan Yang, Gyuhwa Chung, Faheem Shehzad Baloch
Summary: This study focused on exploring the genetic diversity and population structure of 90 rosewood accessions collected from different localities in the Peruvian Amazon region using DArTseq markers. Results showed significant genetic variations within populations, with the Mairiricay population demonstrating the highest genetic diversity. Accessions Tamshiyacu-2 and Mairiricay-15 were identified as genetically distinct. The diversity indices indicated that subpopulation A displayed the highest diversity among the populations.
Article
Ornithology
Jacob C. Cooper, J. Dylan Maddox, Kellie McKague, John M. Bates
Summary: The study reveals that the Afromontane bird species C. reichenowi exhibits regional diversification in geographically disjunct areas, indicating that ecological diversity is shaping populations in Central Africa.
Article
Biology
Haley E. Hanson, Cedric Zimmer, Bilal Koussayer, Aaron W. Schrey, J. Dylan Maddox, Lynn B. Martin
Summary: The study reveals that house sparrows with high EP express more TLR4 in various physiological tissues, especially in the blood, compared to those with low EP. Additionally, only females with high EP exhibit reversibility in gene expression. The effect of EP varies between sexes and among tissues.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Faheem Shehzad Baloch, Stalin Juan Vasquez Guizado, Muhammad Tanveer Altaf, Ilker Yuce, Yeter Cilesiz, Mehmet Bedir, Muhammad Azhar Nadeem, Rustu Hatipoglu, Juan Carlos Castro Gomez
Summary: The study revealed high genetic diversity in rosewood germplasm and a significant portion of the genetic variation within populations. The iPBS-retrotransposon marker system proved to be useful in exploring genetic diversity in Peruvian rosewood. The STRUCTURE algorithm separated the germplasms into different population groups based on their geographic locations.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Health Care Sciences & Services
Carlos de las Cuevas, Luis Gutierrez-Rojas, Miguel A. Alvarez-Mon, Alvaro Andreu-Bernabeu, Luis Capitan, Juan Carlos Gomez, Iria Grande, Diego Hidalgo-Mazzei, Raimundo Mateos, Pedro Moreno-Gea, Tomas De Vicente-Munoz, Francisco Ferre
Summary: The study showed that an online educational intervention significantly improved psychiatrists' awareness, knowledge and attitudes toward telepsychiatry, but did not have a significant impact on their skills.
TELEMEDICINE AND E-HEALTH
(2023)
Article
Multidisciplinary Sciences
Marianela Cobos, Segundo L. Estela, Hicler N. Rodriguez, Carlos G. Castro, Miguel Grandez, Juan C. Castro
Summary: This report presents shotgun metagenomics sequencing data from soils of a highly dissected low hill in the western Amazonia region, focusing on the sample collection, DNA purification, library preparation, and sequencing methods. The soils in this region are composed of moderately fertile sediments, but scientific information about soil microbial and functional diversity is still lacking.
Article
Plant Sciences
Jhon A. Vargas, Diego A. Leonardo, Humberto D'Muniz Pereira, Adriana R. Lopes, Hicler N. Rodriguez, Marianela Cobos, Jorge L. Marapara, Juan C. Castro, Richard C. Garratt
Summary: In plants, the synthesis of ascorbic acid involves multiple metabolic pathways. This study presents the crystal structure of an L-galactose dehydrogenase from spinach, which is involved in the D-mannose/L-galactose pathway. The enzyme has similar kinetic parameters to its counterpart in camu camu, a vitamin C-rich fruit. The structure reveals insights into substrate specificity and the oxidation reaction leading to ascorbic acid accumulation. The findings contribute to a better understanding of the structure-function relationships in vitamin C synthesis enzymes.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Immunology
Laura Gutierrez-Soriano, Juan Carlos Kling Gomez, Nicolas Maya Trujillo, Eduardo Becerra Zapata
Summary: This case report highlights the importance of recognizing and diagnosing hypoxic hepatitis in patients with systemic hypoperfusion risk factors, even after lung transplantation. The study emphasizes the high mortality rate associated with this condition and the lack of reported cases of liver failure secondary to hypoxic hepatitis after lung transplantation.
TRANSPLANTATION PROCEEDINGS
(2022)
Article
Genetics & Heredity
Marianela Cobos, Ruth C. Condori, Miguel A. Grandez, Segundo L. Estela, Marjorie T. Del Aguila, Carlos G. Castro, Hicler N. Rodriguez, Jhon A. Vargas, Alvaro B. Tresierra, Luis A. Barriga, Jorge L. Marapara, Pedro M. Adrianzen, Roger Ruiz, Juan C. Castro
Summary: In this study, the genome features and biochemical profile of Synechococcus sp. UCP002, a cyanobacterium isolated from the Peruvian Amazon Basin region, were determined. The results showed that this strain had a high growth rate and acceptable genome quality parameters. The genome contained genes for metalloregulator transcription factors involved in sensing heavy metal pollution, as well as genes for prokaryotic defense systems. Biochemical analysis revealed the production of essential nutrients and important compounds. This strain has potential biotechnological applications.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Juan C. Castro, J. Dylan Maddox, Hicler N. Rodriguez, Carlos G. Castro, Sixto A. Iman-Correa, Marianela Cobos, Jae D. Paredes, Jorge L. Marapara, Janeth Braga, Pedro M. Adrianzen
Article
Multidisciplinary Sciences
Marianela Cobos, Hicler N. Rodriguez, Segundo L. Estela, Carlos G. Castro, J. Dylan Maddox, Jae D. Paredes, Juan R. Saldana, Alvaro B. Tresierra, Juan C. Castro