Journal
BIOELECTROCHEMISTRY
Volume 101, Issue -, Pages 35-41Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.06.013
Keywords
Electrochemical sensors; DNA-based biosensors; Metal ion determination
Funding
- Polish Ministry of Science and Higher Education [IP2012 064972]
- Warsaw University of Technology
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The possibility of utilization of gold electrodes modified with short guanine-rich ssDNA probes for determination Of Pb2+ was examined. Interaction between guanine residues and lead ion followed by formation of G-quadruplex structures was confirmed by electrochenlical impedance spectroscopy investigations. An external cationic redox label, methylene blue, was employed in voltammetric measurements for analytical signal generation. It was shown that due to the G-quadruplex formation, the oligonucleotides in the recognition layer fold, which enhances the electron transfer between methylene blue and the electrode surface. The MB current signal rises proportionally to the lead ion concentration in the range from 0.05 to 1 mu mol/L. The developed biosensor demonstrated high selectivity towards Pb2+ ion, with only minor response towards interfering metal cations. The calculated limit of detection was of 34.7 nmol/L The utilization of the biosensor for Pb2+ determination in real samples of water was also tested. (C) 2014 Elsevier B.V. All rights reserved.
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