Article
Food Science & Technology
Yongpeng Guo, Yanan Wang, Yu Tang, Qiugang Ma, Cheng Ji, Lihong Zhao
Summary: This study investigates the detoxification potential of dye-decolorizing peroxidase BsDyP from Bacillus subtilis 168 on the mycotoxin Zearalenone (ZEN) commonly found in food. Computational and experimental analyses suggest that BsDyP can bind ZEN and oxidize it using H2O2 as an electron acceptor. Addition of ABTS as a redox mediator further enhances ZEN degradation, and the transformation product shows reduced estrogenicity. The application of a bi-enzyme system achieves a significant degradation percentage of ZEN in contaminated corn steep liquor. These findings suggest the potential use of BsDyP as a biocatalyst for detoxification of ZEN in food and feed.
Article
Biochemistry & Molecular Biology
Xinge Xu, Min Pang, Jiawei Liu, Yuping Wang, Xiaoyan Wu, KunLun Huang, Zhihong Liang
Summary: Bacillus subtilis CW14, isolated from fresh elk droppings in Beijing Zoo, is a GRAS bacterium capable of detoxifying ochratoxin A (OTA). The genome sequence of CW14 revealed putative enzymes involved in degrading mycotoxins, with particular focus on carboxypeptidases for OTA detoxification. This bacterium and its carboxypeptidases show promise as OTA detoxification agents in food and feed industry production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Muhammad Bilal, Caroline Achard, Florence Barbe, Eric Chevaux, Jennifer Ronholm, Xin Zhao
Summary: Probiotics help young chicks achieve early maturation of intestinal microbiota, preventing a decline in microbial diversity, promoting the increase of beneficial microbes, and improving gut microbial balance.
Article
Biochemistry & Molecular Biology
Jonathan Jagodnik, Brian Tjaden, Wilma Ross, Richard L. Gourse
Summary: Ligand-binding RNAs, known as RNA aptamers, are abundant in all three domains of life and play a role in sensing metabolites and small molecules. This study introduces a method called RNA-DRaCALA, which allows for the rapid screening of numerous RNA aptamers by examining their ligand binding. Using this method, 30 RNA aptamers were screened for their ability to bind (p)ppGpp, and key features distinguishing between ppGpp and pppGpp binding sites were identified. Additionally, the method was shown to be effective in analyzing binding of aptamers with thiamine pyrophosphate.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Tamara Hoffmann, Devid Mrusek, Patricia Bedrunka, Fabiana Burchert, Christopher-Nils Mais, Daniel B. Kearns, Florian Altegoer, Erhard Bremer, Gert Bange
Summary: Biofilm formation in Bacillus subtilis requires the expression of matrix production genes, which are upregulated by the transcriptional activator RemA. The study revealed that RemA forms octameric rings with the potential to form a 16-meric superstructure, and it can wrap DNA through a LytTR-related domain.
NATURE COMMUNICATIONS
(2021)
Article
Agriculture, Dairy & Animal Science
Panagiotis Ballas, Christoph Gabler, Karen Wagener, Marc Drillich, Monika Ehling-Schulz
Summary: The pathogenicity of B. pumilus strains isolated from cows with and without endometritis was evaluated in this study using a cell-culture-based approach. These strains were found to possess specific genes related to enzyme production and caused damage to endometrial bovine cells in a dose-dependent manner. These findings have important implications for understanding the role of this pathogen in the disease.
Article
Biotechnology & Applied Microbiology
Mathieu Bouchard-Rochette, Younes Machrafi, Louis Cossus, Thi Thuy An Nguyen, Hani Antoun, Arnaud Droit, Russell J. Tweddell
Summary: Bacillus pumilus strain PTB180 and Bacillus subtilis strain PTB185 secrete lipopetides that have strong antagonistic activity against several plant pathogens, including Botrytis cinerea. These strains inhibit the mycelial growth and conidial germination of B. cinerea and can survive on tomato phyllosphere for at least 21 days. Foliar applications of these strains significantly reduce the incidence and severity of gray mold on tomato plants.
BIOLOGICAL CONTROL
(2022)
Article
Biochemistry & Molecular Biology
Aisling Brady, Nuria Quiles-Puchalt, Francisca Gallego del Sol, Sara Zamora-Caballero, Alonso Felipe-Ruiz, Jorge Val-Calvo, Wilfried J. J. Meijer, Alberto Marina, Jose R. Penades
Summary: Some Bacillus-infecting bacteriophages utilize a peptide-based communication system, called arbitrium, to coordinate the lysis-lysogeny decision. AimR, a key player in this system, is involved in a complex network of regulation. The SP beta phage, possessing a functional arbitrium system, can optimize production of infective particles while preserving the number of surviving cells, which increases phage persistence in nature.
Article
Microbiology
Huihui Wan, Xiaoying Ouyang, Ting Yang, Tianyun Ye, Minfei Jin, Jing Huang
Summary: In this study, the 4GT gene was cloned and expressed, and its catalytic function and expression conditions were investigated. High yield of recombinant 4GT enzyme was obtained through optimization of fermentation process. This study provides valuable references for the characterization and expression of food-grade enzymes.
CURRENT MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Huiling Jia, Shengwei Huang, Shuo Cheng, Xiwen Zhang, Xue Chen, Yisen Zhang, Jun Wang, Lifang Wu
Summary: This study systematically analyzed the mechanisms of selenium reduction by the probiotic bacterium Bacillus subtilis both in vivo and in vitro, revealing multiple key genes and pathways involved. The findings provide new insights for the treatment of selenite.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Min-Kyung Park, Soyeon Lee, Young-Suk Kim
Summary: The study investigated the changes in volatile metabolites of Bacillus subtilis by exposing it to different culture conditions, revealing alterations in metabolic pathways such as carbohydrate metabolism, amino acid metabolism, fatty acid metabolism, and leucine degradation. The results showed culture condition-specific metabolic changes, particularly in secondary volatile metabolites related to the sensory property of foods.
Article
Horticulture
Mujahid Rasool, Adnan Akhter, Muhammad Saleem Haider
Summary: This study aimed to investigate the impact of biochar on tomato plants' defense mechanisms against Alternaria solani by inducing the expression of defense related genes. Results showed that 6% green waste biochar was more efficient in enhancing the resistance of tomatoes, indicating the importance of biochar type and application rate in eliciting defense responses. Additionally, the combination of biochar and PGPR enhanced protection against early blight by involving both induced systemic resistance (ISR) and systemic acquired resistance (SAR) mechanisms.
SCIENTIA HORTICULTURAE
(2021)
Article
Mathematics, Applied
Jianyu Hu, Xiaoli Chen, Jinqiao Duan
Summary: We investigate the quantitative network of gene expression dynamics in the competence development of Bacillus subtilis, quantifying the most probable transition pathways for both excitable and bistable dynamics and analyzing how noise intensity affects the transition phenomena.
Article
Microbiology
A. O. Koryagina, F. R. Osmanova, A. A. Toymentseva, A. Laikov, M. R. Sharipova
Summary: A study comparing the expression of B. pumilus extracellular serine proteinases under different promoters and signal peptides revealed that the selection of optimal signal peptides is crucial for individual secreted proteins.
Article
Biotechnology & Applied Microbiology
Peter Klausmann, Lars Lilge, Moritz Aschern, Katja Hennemann, Marius Henkel, Rudolf Hausmann, Kambiz Morabbi Heravi
Summary: This study investigated the impact of introducing mutations from strain JABs32 into B. subtilis KM1016 on surfactin production performance. The results showed that deletion of spo0A led to increased growth rates but lowered surfactin titers, while deletion or elongation of abrB resulted in decreased growth rates but higher surfactin yields. The genetic differences between B. subtilis KM1016 and JABs32 offer new and improved fermentation methods for high cell density processes.
MICROBIAL CELL FACTORIES
(2021)
