Article
Biochemistry & Molecular Biology
Alexandra Catalina Birca, Oana Gherasim, Adelina-Gabriela Niculescu, Alexandru Mihai Grumezescu, Ionela Andreea Neacsu, Cristina Chircov, Bogdan Stefan Vasile, Ovidiu Cristian Oprea, Ecaterina Andronescu, Miruna Silvia Stan, Carmen Curutiu, Lia Mara Ditu, Alina Maria Holban
Summary: The use of a microfluidic platform with a cross-shape design was explored in this study for synthesizing silver nanoparticles. The precursor concentration and purging speed were varied as experimental parameters. The obtained samples were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Alginate-based hydrogels containing hyaluronic acid and silver nanoparticles were successfully synthesized and evaluated for their antimicrobial activity and wound treatment efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Adelina-Gabriela Niculescu, Alexandru Mihai Grumezescu
Summary: Chitosan and alginate, two natural polymers, have attracted significant interest for their various applications in nano form. They have shown potential in biomedical, environmental protection, and food industry fields, serving as delivery vehicles, antimicrobial agents, and more. This paper discusses the latest applications of chitosan-alginate-based nanomaterials and serves as a starting point for further research.
Review
Engineering, Environmental
Showkat Ali Ganie, Luqman Jameel Rather, Qing Li
Summary: Polysaccharide biomaterials with carbohydrate backbones have been extensively studied for their potential applications in anticancer treatments. Compared to other substances, polysaccharides offer advantages such as natural availability, easy absorption, favorable immunogenicity, biocompatibility, and biodegradability. However, the naturally obtained polysaccharides may have limitations in their structures, necessitating functionalization methods to expand their applications. A variety of polysaccharide biomaterials, including chitosan, pectin, alginate, hyaluronic acid, dextran, and inulin, have been investigated as drug delivery vehicles or biomaterials for anticancer purposes. The review summarizes recent strategies for the development of polysaccharide biomaterials and discusses the influence of bioactive molecules on their anticancer properties.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2023)
Review
Polymer Science
Lisa Efriani Puluhulawa, I. Made Joni, Khaled M. Elamin, Ahmed Fouad Abdelwahab Mohammed, Muchtaridi Muchtaridi, Nasrul Wathoni
Summary: Chitosan-hyaluronic acid nanoparticles have the potential to be an effective and targeted treatment for various types of cancer, with good characteristics and active targeting capabilities.
Article
Nanoscience & Nanotechnology
Zhongshan Wang, Guangsheng Wu, Zhujun Yang, Xuejian Li, Zhihong Feng, Yimin Zhao
Summary: In this study, miR-21-loaded chitosan/tripolyphosphate/hyaluronic acid nanoparticles were prepared and formed a stable biological seal on titanium surfaces. The miR-21-functionalized titanium surface enhanced fibroblast adhesion, proliferation, and expression of extracellular matrix-related genes.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Pharmacology & Pharmacy
Enrica Chiesa, Antonietta Greco, Federica Riva, Rossella Dorati, Bice Conti, Tiziana Modena, Ida Genta
Summary: Hyaluronic acid-based nanoparticles can deliver protein cargo to cells overexpressing HA receptors, playing a crucial role in managing meshwork formation, cell interactions, and protein entrapment efficacy. Using microfluidic technology, the research focused on the impact of HA molecular weight on NPs formation.
Article
Environmental Sciences
Afsana Sheikh, Sahim Aziz Hazari, Nagashekhara Molugulu, Saad Ali Alshehri, Shadma Wahab, Amirhossein Sahebkar, Prashant Kesharwani
Summary: This article investigates the potential of chitosan nanoparticles loaded with gallic acid (GA) for the treatment of psoriasis. The study found that coating the nanoparticles with hyaluronic acid (HA) slightly increased the particle size but enhanced their inhibitory effect on cells. In vivo experiments demonstrated the potential of HA-modified nanoparticles in restricting psoriasis. Further research should explore the application of HA-modified nanoparticles in the treatment of other skin-related conditions.
ENVIRONMENTAL RESEARCH
(2023)
Article
Nanoscience & Nanotechnology
Sara Gimondi, Rui L. Reis, Helena Ferreira, Nuno M. Neves
Summary: In this study, a microfluidic method was proposed to synthesize chitosan-hyaluronic acid nanoparticles (NPs), which showed smaller and more uniform size compared to the traditional dropwise method. The obtained NPs demonstrated potential in reducing inflammatory molecules in macrophages and fibroblasts. This study provides a new approach for the synthesis of nanocarriers based on bioactive macromolecules.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Polymer Science
Lisna Meylina, Muchtaridi Muchtaridi, I. Made Joni, Khaled M. Elamin, Nasrul Wathoni
Summary: This study investigates the potential of chitosan nanoparticles coated with hyaluronic acid for targeted delivery of alpha mangostin (AM) against MCF-7 breast cancer cells. The results show that AM-CS/HA has a spherical shape with average diameter of 304 nm, a polydispersity index of 0.3, and a negative charge of 24.43 mV. The encapsulation efficiency and drug loading were high, and AM released from AM-CS/HA at an acidic pH showed sustained release and higher cytotoxic effect on MCF-7 cells compared to AM nanoparticles without HA coating and AM alone.
Article
Biophysics
Ji Wang, Wei He, Tao Wang, Man Li, Xinsong Li
Summary: By adding 30 mg/L SM-Fe NPs, significant improvement in the production of hyaluronic acid (HA) was observed, with the dry weight of HA produced increasing by 3.28 times compared to the control group, presenting promising opportunities for industrial production.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Maghsoud Amirfarhangi Anbardan, Siamak Alipour, Gholam Reza Mahdavinia, Parisa Fathi Rezaei
Summary: The magnetic nanocarriers composed of chitosan/hyaluronic acid complexed with kappa-carrageenan were successfully synthesized and demonstrated as an efficient drug delivery system. The nanodelivery system showed high drug loading capacity and responsive drug release behavior under pH stimuli, while exhibiting no inhibitory effect on cell growth.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Mengying Zhang, Huimin Yu, Jinglu Hu, Zhengyu Zhao, Lei Liu, Gaomin Yang, Tingli Wang, Guang Han, Shiyong Song
Summary: This study prepared a nanocarrier based on hyaluronic acid and solanesol, which showed great potential in cancer therapy. Loading doxorubicin into the nanocarrier enabled pH-sensitive drug release, with lower cardiotoxicity and hepatotoxicity compared to free doxorubicin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Chemistry, Medicinal
Hussein H. Genedy, Thierry Delair, Alexandra Montembault
Summary: Vectorization of microRNAs using polymeric nanoparticles, particularly chitosan, has great potential for treating various diseases. Chitosan, being biodegradable and biocompatible, can easily encapsulate microRNA and form polyplexes. However, fine tuning of chitosan's characteristics is crucial for successful formulation. This review summarizes chitosan microRNA formulations studied in the past decade, highlighting differences in materials, formulation processes, and intended applications. Factors contributing to the superiority of optimized systems are also discussed to maximize the potential of chitosan microRNA nanocarriers.
Article
Chemistry, Physical
Suhas Gotla, Silvina Matysiak
Summary: Neurodegeneration in Alzheimer's disease is caused by abnormal accumulation of amyloid-beta (Aβ) peptides. Chitosan (CHT), a macromolecule, has been found to inhibit the aggregation of Aβ and reduce neurotoxicity. Molecular dynamics simulations showed that CHT binds and sequesters Aβ peptides, limiting their aggregation. CHT also reshapes the conformational landscapes of Aβ peptides, depending on its concentration and cationicity, which could guide the development of CHT-based materials for Alzheimer's treatment.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Tongyao Zhao, Xiaoyan Li, Yumei Gong, Yanzhu Guo, Fengyu Quan, Qiang Shi
Summary: The study focused on the binary blending of sodium alginate and quaternary ammonium chitosan to fabricate composite fibers. The results showed changes in solution state and electrical potential at different mass ratios, leading to the production of fibers with excellent properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Instruments & Instrumentation
Sara Basile, Essyrose Mathew, Ida Genta, Bice Conti, Rossella Dorati, Dimitrios A. Lamprou
Summary: This study validated an optimized FDM 3D printing method for the manufacturing of personalized medical implants, particularly fistulas. Different types of filaments were produced using hot melt extrusion, and the printed fistulas were characterized using various physicochemical analysis methods. The results demonstrated that TPU material is suitable for 3D printing and successful drug loading. Drug release testing showed sustained release, meeting the requirements for implants in the patient's body.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Erika M. M. Tottoli, Laura Benedetti, Enrica Chiesa, Silvia Pisani, Giovanna Bruni, Ida Genta, Bice Conti, Gabriele Ceccarelli, Rossella Dorati
Summary: This study proposes a new electrospun dressing called Biofiber as a solution to prevent hypertrophic scar formation in complex wounds. Biofiber has excellent wetting properties and good absorbency and moisture vapor transmission rate. By controlling the release of the natural antifibrotic agent Naringin, Biofiber can demonstrate prolonged antifibrotic effects on normal human dermal fibroblasts. However, the effect on inhibiting hypertrophic scar formation is not significant.
Article
Biochemistry & Molecular Biology
Mariella Rosalia, Pietro Grisoli, Rossella Dorati, Enrica Chiesa, Silvia Pisani, Giovanna Bruni, Ida Genta, Bice Conti
Summary: Vascular graft infections are a severe complication in vascular surgery, with high morbidity and mortality. Currently, there are no commercially available infection-proof small-diameter vascular grafts. In this study, two prototypes of small-diameter vascular grafts loaded with tobramycin were developed and tested for their antimicrobial activity. The prototypes showed sustained and diffusive driven drug release, with Prototype 1 being more efficient in killing bacterial populations. Overall, both prototypes exhibited good antimicrobial activity over the 5 days of in vitro testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Silvia Pisani, Deborah Di Martino, Silvia Cerri, Ida Genta, Rossella Dorati, Giulia Bertino, Marco Benazzo, Bice Conti
Summary: In this study, four different active encapsulation methods were compared for loading a model protein into liposomes. Electroporation yielded the highest encapsulation efficiency, but led to a significant increase in liposome size. Freeze-thawing was an effective method, allowing for high encapsulation efficiency while maintaining suitable size. Charged liposomes showed slower in vitro release and good cytocompatibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Erika Maria Tottoli, Laura Benedetti, Federica Riva, Enrica Chiesa, Silvia Pisani, Giovanna Bruni, Ida Genta, Bice Conti, Gabriele Ceccarelli, Rossella Dorati
Summary: This study proposes a new type of dressing, Biofiber PF, loaded with Pirfenidone, for the treatment of HTSs in complex wounds. It has antifibrotic properties and promotes physiological healing by modulating the fibrotic process.
Article
Pharmacology & Pharmacy
Silvia Pisani, Valeria Mauri, Erika Negrello, Valeria Friuli, Ida Genta, Rossella Dorati, Giovanna Bruni, Stefania Marconi, Ferdinando Auricchio, Andrea Pietrabissa, Marco Benazzo, Bice Conti
Summary: In this study, hybrid scaffolds were developed as a versatile platform for soft tissue regeneration. These scaffolds exhibited favorable cell adhesion and proliferation, and allowed for controlled drug release.