Article
Biochemical Research Methods
F. Ceciliani, G. Avila Morales, G. De Matteis, F. Grandoni, R. Furioso Ferreira, P. Roccabianca, C. Lecchi
Summary: Monocytes and macrophages in bovines play important roles in key diseases, but their functions and purification procedures differ from that of their human counterparts. This review discusses the current knowledge of bovine monocytes and macrophages, including methods for their purification and characterization of major functions. Various techniques such as flow cytometry, cytology, histology, and immunohistochemistry are utilized to describe their populations and functions.
Article
Clinical Neurology
Anan Shtaya, Leslie R. Bridges, Rebecca Williams, Sarah Trippier, Liqun Zhang, Anthony C. Pereira, James A. R. Nicoll, Delphine Boche, Atticus H. Hainsworth
Summary: The study identified an anti-inflammatory process occurring alongside neuroinflammation post-sICH in clinical patients, involving native microglia and blood monocytes. This novel pathway offers therapeutic targets and a treatment opportunity window for delivering therapeutics through invading monocytes during 3-5 days post-sICH.
Article
Biochemistry & Molecular Biology
Giacomo Della Camera, Tinghao Liu, Wenjie Yang, Yang Li, Victor F. Puntes, Sabrina Gioria, Paola Italiani, Diana Boraschi
Summary: The study found that nanoparticles alone cannot generate memory, while LPS induces a tolerance memory response. Nanoparticles have no significant effect on LPS-induced tolerance memory, but may have donor-specific effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Mayra M. Ferrari Barbosa, Alex Issamu Kanno, Leonardo Paiva Farias, Mariusz Madej, Gergo Sipos, Silverio Sbrana, Luigina Romani, Diana Boraschi, Luciana C. C. Leite, Paola Italiani
Summary: The study demonstrates that particulate challenges are more efficient than soluble molecules in inducing innate immune cell memory response, which helps better protect the body and improve reactivity to future challenges.
Review
Immunology
Lisa Hirahara, Kaoru Takase-Minegishi, Yohei Kirino, Yuki Iizuka-Iribe, Yutaro Soejima, Ryusuke Yoshimi, Hideaki Nakajima
Summary: Behcet's disease is a systemic inflammatory disease characterized by recurrent oral ulcers, genital ulcers, cutaneous inflammation, and uveitis. The pathogenesis of BD is not fully understood, but it is likely a result of genetic susceptibility and environmental factors. This review discusses the roles of monocytes and macrophages in BD and their potential as therapeutic targets.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Patricia Urban, Paola Italiani, Diana Boraschi, Sabrina Gioria
Summary: The study investigates the interaction of SARS-CoV-2 with the human immune system, finding that monocytes pre-exposed to the N protein exhibit a milder response to subsequent viral or bacterial challenges, potentially leading to a mild response to new infections.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Melissa Prat, Kimberley Coulson, Clement Blot, Godefroy Jacquemin, Mathilde Romano, Marie-Laure Renoud, Mohamad AlaEddine, Augustin Le Naour, Helene Authier, Mouna Chirine Rahabi, Khaddouj Benmoussa, Marie Salon, Melissa Parny, Jean-Pierre Delord, Gwenael Ferron, Lise Lefevre, Bettina Couderc, Agnes Coste
Summary: In this study, it was found that activation of PPARγ in treated small peritoneal macrophages can inhibit ovarian adenocarcinoma growth, regulate the proportion of immune cells, and attenuate the immunosuppressive properties of tumor-associated macrophages.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Immunology
Robb Wesselingh, Sarah Griffith, James Broadley, David Tarlinton, Katherine Buzzard, Udaya Seneviratne, Helmut Butzkueven, Terence J. O'Brien, Mastura Monif
Summary: Autoimmune encephalitis (AE) is an inflammatory disease of the central nervous system that can cause long-term seizures and cognitive dysfunction. The role of the innate immune system in AE is not well understood. This study investigated the involvement of the innate immune system in AE by evaluating peripheral monocytes and serum cytokines.
JOURNAL OF AUTOIMMUNITY
(2023)
Review
Biochemistry & Molecular Biology
Nadia Lampiasi
Summary: Mast cells and macrophages are innate immune cells that play important roles in maintaining tissue homeostasis and responding to inflammatory conditions. Their crosstalk is essential for optimal coordination of their functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Magda Lewandowska, Ton Sharoni, Yael Admoni, Reuven Aharoni, Yehu Moran
Summary: The study characterized the antiviral immune response of the cnidarian Nematostella vectensis and showed that its RLR paralogs exhibit features distinctive for both vertebrate and invertebrate systems. The functional evidence provided supports the conserved role of RLRs in initiating immune response to dsRNA before the cnidarian-bilaterian split, laying a foundation for further research on the evolution of immune responses to RNA viruses.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Review
Physiology
Tlili Barhoumi, Stephen Todryk
Summary: The renin-angiotensin system (RAS) is a central regulator of cardiovascular physiology, and its hyper-activation is common in hypertension. Recent studies have highlighted the crucial role of the immune system, particularly monocyte/macrophage cells, in hypertension and its related pathology. Targeting RAS components in these cells may present an innovative strategy for the treatment of hypertension.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Benjamin J. Swartzwelter, Alessandro Verde, Laura Rehak, Mariusz Madej, Victor. F. Puntes, Anna Chiara De Luca, Diana Boraschi, Paola Italiani
Summary: Understanding the interaction between human monocytes/macrophages and engineered nanoparticles is crucial for assessing particle safety and exploring their potential medical uses. The study compared traditional 2D cell cultures with 3D collagen matrix cultures to evaluate the effects of gold nanoparticles on monocyte activation and innate memory. The results showed that while there were similarities in the response to stimuli between 2D and 3D cultures, significant differences were observed in the memory response of cells primed with nanoparticles.
Article
Immunology
Sara Francisco, Alicia Arranz, Javier Merino, Carmen Punzon, Rosario Perona, Manuel Fresno
Summary: The study found that TLR2 ligands activate MAPKs p38 and ERK earlier in macrophages, leading to higher levels of IL-10/IL-12 and IL-10/TNF-alpha ratios compared to TLR4 ligand LPS. Early TLR2-mediated p38 induction contributes to high IL-10 production, likely suppressing host Th1 response against certain pathogens.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Sara Francisco, Jean-Marc Billod, Javier Merino, Carmen Punzon, Alicia Gallego, Alicia Arranz, Sonsoles Martin-Santamaria, Manuel Fresno
Summary: This study reveals that Ochrobactrum intermedium LPS can activate both TLR4 and TLR2, indicating the occurrence of TLR4/TLR2 heterodimerization, and the core saccharide plays an important role in this interaction.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Zi Zhou, Hao Wang, Sipin Tan, Huali Zhang, Yaxi Zhu
Summary: Diabetes mellitus is a globally prevalent metabolic inflammatory disease that increases the risk of various infections. This paper focuses on the enhanced severity of infections in diabetes patients and the innate immune alterations associated with these changes, aiming to better understand the influence of diabetes on susceptibility, pathophysiology, and clinical outcomes in infections.
Article
Cardiac & Cardiovascular Systems
Mariella Bockstahler, Andrea Fischer, Carl Christoph Goetzke, Hannah Louise Neumaier, Martina Sauter, Meike Kespohl, Anna-Maria Mueller, Christin Meckes, Christian Salbach, Mirjam Schenk, Arnd Heuser, Ulf Landmesser, January Weiner, Benjamin Meder, Lorenz Lehmann, Adelheid Kratzer, Karin Klingel, Hugo A. Katus, Ziya Kaya, Antje Beling
Letter
Allergy
Banu Orak, Gonza Ngoumou, Frederic Ebstein, Barbara Zieba, Carl Christoph Goetzke, Ellen Knierim, Angela M. Kaindl, Axel Panzer, Manuela Theophil, Monika Berns, Elke Krueger, Christian Meisel, Nadine Unterwalder, Tilmann Kallinich
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ahmet Hazini, Babette Dieringer, Markian Pryshliak, Klaus-Peter Knoch, Lisanne Heimann, Beatrice Tolksdorf, Kathleen Pappritz, Muhammad El-Shafeey, Michele Solimena, Antje Beling, Jens Kurreck, Karin Klingel, Henry Fechner
Summary: Insertion of target sites complementary to miR-375 and miR-1 into the oncolytic virus genome can prevent pancreas and heart toxicity, allowing selective attack on colorectal carcinoma cells. The engineered viruses showed reduced replication in cell lines expressing the corresponding microRNAs, leading to improved safety and therapeutic efficiency in mouse models.
HUMAN GENE THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Janik Boehnke, Sandra Pinkert, Maria Schmidt, Hans Binder, Nicole Christin Bilz, Matthias Jung, Uta Reibetanz, Antje Beling, Dan Rujescu, Claudia Claus
Summary: This study discusses the potential association of enteroviruses with pregnancy complications, including miscarriages, and characterizes the infection of human induced pluripotent stem cells and their derived cells by coxsackievirus B3. The research shows that CVB3 infection reduces pluripotency gene expression and mesodermal cells are particularly vulnerable to infection. This highlights the importance of considering enteroviruses in pregnancy screening programs and suggests that iPSCs could be a valuable tool for studying viral infections in specific target organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Carl Christoph Goetzke, Nadine Althof, Hannah Louise Neumaier, Arndt Heuser, Ziya Kaya, Meike Kespohl, Karin Klingel, Antje Beling
Summary: The preclinical model of troponin I-induced myocarditis revealed the significant role of the immunoproteasome (ip) in heart-directed autoimmunity, with viral infection enhancing systemic inflammatory responses. However, targeting the ip with inhibitor ONX 0914 did not alter heart tissue damage or cardiac function in CV-triggered myocarditis, suggesting that ip inhibitors may be effective in treating this condition by modulating systemic inflammation.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Review
Medicine, General & Internal
Stylianos Tomaras, Carl Christoph Goetzke, Tilmann Kallinich, Eugen Feist
Summary: Adult-onset Still's disease is a rare systemic autoinflammatory disease characterized by arthritis, spiking fever, skin rash, and elevated ferritin levels. Immune dysregulation plays a central role in the disease, involving pathogenic involvement of both arms of the immune system. Extensive research has led to a better understanding of AoSD and revolutionary advances in treatment.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Medicine, General & Internal
Carl Christoph Goetzke, Frederic Ebstein, Tilmann Kallinich
Summary: The UPS plays a crucial role in cellular functions, such as regulating protein homeostasis and MHC I antigen processing. In humans, loss-of-function mutations in the proteasome can lead to autoinflammation, while abnormally increased proteasomal activity is also linked to other inflammatory diseases.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Virology
Sandra Pinkert, Meike Kespohl, Nicolas Kelm, Ziya Kaya, Arnd Heuser, Karin Klingel, Antje Beling
Summary: The study demonstrates that the use of tramadol in the CVB3 myocarditis mouse model does not have disruptive effects on viral pathogenicity or immune response in the heart. This supports the consideration of the demonstrated protocol as a general analgesic strategy for CVB3 infection.
Article
Virology
Martin Voss, Vera Braun, Clara Bredow, Peter-Michael Kloetzel, Antje Beling
Summary: Infection with coxsackievirus B3 results in impaired protein homeostasis and accumulation of ubiquitinylated proteins, attributed to viral proteins 2B and 3A. During the exponential replication phase, oxidative damage of membrane proteins is reduced due to the recruitment of glutathione. The proteasome is shown to play a role in processing viral precursor proteins during infection.
Article
Biochemistry & Molecular Biology
Martin Voss, Gunnar Kleinau, Niclas Gimber, Katharina Janek, Clara Bredow, Fabien Thery, Francis Impens, Jan Schmoranzer, Patrick Scheerer, Peter-Michael Kloetzel, Antje Beling
Summary: Through mutation analysis and biochemical characterization, we found that the enteroviral transmembrane protein 3A can form disulfide-linked dimers via a conserved cysteine residue, which plays an important role in the stability and functional integrity of the protein.
Article
Immunology
Deeksha Munnur, Qiwen Teo, Denzel Eggermont, Horace H. Y. Lee, Fabien Thery, Julian Ho, Sophie Wilhelmina van Leur, Wilson W. S. Ng, Lewis Y. L. Siu, Antje Beling, Hidde Ploegh, Adan Pinto-Fernandez, Andreas Damianou, Benedikt Kessler, Francis Impens, Chris Ka Pun Mok, Sumana Sanyal
Summary: Viruses can modulate ISGylation to affect macrophage responses, leading to exaggerated inflammation in COVID-19. Different viruses induce varying responses related to ISG15, with SARS-CoV-2 preferentially triggering deISGylation to generate free ISG15.
Article
Multidisciplinary Sciences
Fabien Thery, Lia Martina, Caroline Asselman, Heidi Repo, Yifeng Zhang, Koen Sedeyn, George D. Moschonas, Clara Bredow, Qi Wen Teo, Jingshu Zhang, Madeleine Vessely, Kevin Leandro, Denzel Eggermont, Delphine De Sutter, Katie Boucher, Tino Hochepied, Nele Festjens, Nico Callewaert, Xavier Saelens, Bart Dermaut, Klaus-Peter Knobeloch, Antje Beling, Sumana Sanyal, Lilliana Radoshevich, Sven Eyckerman, Francis Impens
Summary: The researchers identify RNF213 as a sensor for ISGylated proteins on the surface of lipid droplets, showing both antiviral properties and the ability to target intracellular bacteria. RNF213, induced by interferon, exhibits broad antimicrobial activity against various infections and oligomerizes on lipid droplets as a sensor for ISGylated proteins.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Martin Voss, Sandra Pinkert, Meike Kespohl, Niclas Gimber, Karin Klingel, Jan Schmoranzer, Michael Laue, Matthias Gaida, Peter-Michael Kloetzel, Antje Beling
Summary: Enteroviruses are associated with a wide range of clinical manifestations. This study found that the highly conserved amino acid cysteine 38 (C38) in enteroviral protein 3A supports the replication of clinically relevant Coxsackievirus B3 (CVB3) and enhances its virulence.