Review
Biochemistry & Molecular Biology
Chao-Yi Wu, Huang-Yu Yang, Shue-Fen Luo, Jenn-Haung Lai
Summary: RA is a chronic inflammatory disease involving synovial inflammation and articular destruction. RFs, ACPAs, and anti-CarP Abs have been identified as important autoantibodies in predicting and diagnosing RA, with potential implications for treatment response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Aline S. E. Santos, Christine G. Parks, Mariana M. Senna, Armando Meyer
Summary: This study aimed to assess the relationship between pesticide use and antibody levels. The results showed that specific pesticides and fertilizers were associated with antibody levels in the healthy agricultural population.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Pathology
Adrian Y. S. Lee, Dimitra Beroukas, Louise Wienholt, Tom P. Gordon
Summary: The analytical performance of the immunoblot assay for detecting anti-Ro60 was evaluated, with the counterimmunoelectrophoresis method as the gold standard. The study found that the immunoblot assay has high sensitivity and specificity for anti-Ro60 detection. However, the survey of international laboratories revealed that many of them use the manufacturer's recommended cutoff, which may result in missed detection of this clinically important autoantibody.
Article
Immunology
Adrian Y. S. Lee, Joanne H. Reed, Tom P. Gordon
Summary: The diagnostic autoantibodies anti-Ro60 and anti-Ro52/TRIM21 are commonly found in systemic autoimmune disorders like SLE, often co-existing despite lacking physical linkage, signaling a convergence of failed immunological tolerance processes. Sometimes classified together as the SSA or Ro autoantibody system without clear distinction, further research is needed to explore separate detection and reporting for these antibodies and their roles in autoimmunity. Understanding their origins and pathogenic functions will provide insights for preventing and treating autoimmune disorders.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Rheumatology
Federica M. Ucci, Serena Recalchi, Cristiana Barbati, Valeria Manganelli, Antonella Capozzi, Gloria Riitano, Giorgia Buoncuore, Tina Garofalo, Fulvia Ceccarelli, Francesca R. Spinelli, Eugenia Balbinot, Alessandra Celia, Agostina Longo, Cristiano Alessandri, Roberta Misasi, Maurizio Sorice, Fabrizio Conti
Summary: This study investigated the expression of citrullinated and carbamylated proteins in extracellular microvesicles (EMVs) from RA patients. The results showed a significant increase of EMVs and higher expression of citrullinated antigens in RA patients compared to healthy donors, suggesting the involvement of EMVs in the pathogenesis of RA.
Article
Immunology
Pauline Brevet, Claire Lattard, Clement Guillou, Pascal Rottenberg, Patrice Fardellone, Xavier Le-Loet, Thierry Lequerre, Pascal Cosette, Olivier Boyer, Manuel Freret, Olivier Vittecoq
Summary: In patients with early Rheumatoid Arthritis, the gamma chain of carbamylated fibrinogen was identified as a circulating carbamylated target with specific reactivity. ACa-Fib IgG positivity in anti-CCP-negative patients was associated with more inflammatory and erosive disease at baseline, but not with rapid radiological progression.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Daniela Sieghart, Christian Konrad, Sascha Swiniarski, Helmuth Haslacher, Daniel Aletaha, Guenter Steiner
Summary: This study aimed to investigate the diagnostic performance of anti-CCP2 and anti-CCP3 IgG and IgA assays in patients with early RA, with a focus on the prognostic value of IgA ACPA. The results showed that the anti-CCP2 IgG and IgA assays had high specificity but low sensitivity. The anti-CCP3.1 assay had slightly higher sensitivity but lower specificity compared to the anti-CCP2 IgG assay.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Savino Sciascia, Chelsea Bentow, Massimo Radin, Alice Barinotti, Irene Cecchi, Silvia Foddai, Dario Roccatello, Michael Mahler
Summary: This study aimed to explore the use of saliva as an alternative matrix for the detection of autoantibodies. By using a novel and highly sensitive method, the study found a high level of correlation between saliva and serum results, and participants clearly preferred saliva sampling.
Article
Gastroenterology & Hepatology
Johanna Galaski, Christina Weiler-Normann, Miriam Schakat, Kalliopi Zachou, Paolo Muratori, Sibylle Lampalzer, Friedrich Haag, Christoph Schramm, Marco Lenzi, George N. Dalekos, Ansgar W. Lohse
Summary: The study suggests that ANA IFT using HEp-2 cells and ELISA-based autoantibody evaluation may serve as potential alternatives to tissue-based IFT for the diagnosis of autoimmune hepatitis. ELISA testing is also a potentially reliable method for autoantibody assessment in autoimmune hepatitis.
JOURNAL OF HEPATOLOGY
(2021)
Article
Medicine, General & Internal
Pawel Zebryk, Piotr Przymuszala, Jan Krzysztof Nowak, Tomasz Piorunek, Tatiana Mularek-Kubzdela, Mariusz Puszczewicz
Summary: This study evaluated the prevalence and clinical significance of SSc-related autoantibodies and compared the sensitivity of two line immunoblot assays. The findings indicate that different autoantibodies are associated with specific characteristics of SSc.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Rheumatology
Rie Karasawa, Kazuo Yudoh, Toshiko Sato, Megumi Tanaka, Mayumi Tamaki, Sara E. Sabbagh, Terrance P. O'Hanlon, Payam Noroozi-Farhadi, Ira N. Targoff, Willy A. Flegel, Andrew L. Mammen, Frederick W. Miller, Mark D. Hicar, Lisa G. Rider, James N. Jarvis
Summary: This study found that anti-HSC70 autoantibodies are frequently detected in children with JDM and are associated with disease severity. Patients with anti-HSC70 autoantibodies are more likely to develop cutaneous ulcers and require the use of wheelchairs or assistive devices. High scores on measures of myositis damage and an increased number of hospitalizations are also associated with anti-HSC70 autoantibodies.
Article
Geriatrics & Gerontology
Giulio Macchiarella, Vanessa Cornacchione, Celine Cojean, Julia Riker, Yichen Wang, Helene Te, Melanie Ceci, Johann E. Gudjonsson, Swann Gaulis, Jean Francois Goetschy, Audrey Wollschlegel, Stephanie K. Gass, Sofia Oetliker-Contin, Barbara Wettstein-Ling, Dirk J. Schaefer, Pascale Meschberger, Roland de Roche, Rik Osinga, Grazyna Wieczorek, Ulrike Naumann, Joachim C. U. Lehmann, Anna Schubart, Andreas Hofmann, Lukas Roth, Edwin F. Florencia, Christian Loesche, Elisabetta Traggiai, Alexandre Avrameas, Errol P. Prens, Till A. Rohn, Ben Roediger
Summary: Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease characterized by suppurating lesions. In this study, researchers found a high prevalence of antibodies directed against a specific molecule in the blood of HS patients. These antibodies were significantly elevated in HS patients compared to healthy controls and individuals with other inflammatory skin diseases. This suggests an aberration of a specific metabolic pathway and an autoimmune component in the pathogenesis of HS.
AMERICAN JOURNAL OF GERIATRIC PSYCHIATRY
(2023)
Article
Medicine, General & Internal
Vincent Ricchiuti, Kelly Y. Chun, Jane M. Yang, Mary Ann Aure, Luis Gomez, Gary L. Norman, Michael Mahler
Summary: Anti-carbamylated protein (CarP) antibodies can serve as novel markers for the diagnosis and prognosis of rheumatoid arthritis. The study found that anti-CarP antibodies can be detected in both anti-cyclic citrullinated peptide (CCP), rheumatoid factor (RF), and 14-3-3 eta-positive and negative patients, potentially identifying specific subsets of patients.
Article
Medical Laboratory Technology
Liuqing Wang, Li Hua, Xuelian Hong, Fang Chen, Hongwei Du
Summary: This study investigated the association between serum anti-carbamylated protein (anti-CarP) antibodies and disease activity and bone loss in rheumatoid arthritis (RA). The results showed that RA patients had significantly higher levels of serum anti-CarP antibodies compared to non-RA patients and healthy controls, and these levels were positively correlated with disease activity. Furthermore, the concentrations of anti-CarP antibodies were significantly increased in anti-CCP-positive RA patients. There was also a positive correlation between anti-CarP antibodies and RANKL, which is related to bone loss. These findings suggest that anti-CarP antibodies are associated with RA disease activity and may play a role in bone loss associated with RA. The concentration of anti-CarP antibodies could potentially be used as a novel biomarker for early diagnosis of RA.
CLINICA CHIMICA ACTA
(2023)
Review
Rheumatology
Adrian Y. S. Lee
Summary: This review article summarizes the current knowledge of the IgA isotype of anti-double-stranded DNA autoantibodies in systemic lupus erythematosus, focusing on its clinical associations and potential links with lupus nephritis.
Review
Immunology
Nathaniel J. Schuldt, Bryce A. Binstadt
JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Daniel P. Zachs, Sarah J. Offutt, Rachel S. Graham, Yohan Kim, Jerel Mueller, Jennifer L. Auger, Nathaniel J. Schuldt, Claire R. W. Kaisers, Abigail P. Heiiler, Raini Dutta, Hongsun Guo, Jamu K. Alford, Bryce A. Binstadt, Hubert H. Lim
NATURE COMMUNICATIONS
(2019)
Article
Rheumatology
Lauren A. Henderson, Scott W. Canna, Grant S. Schulert, Stefano Volpi, Pui Y. Lee, Kate F. Kernan, Roberto Caricchio, Shawn Mahmud, Melissa M. Hazen, Olha Halyabar, Kacie J. Hoyt, Joseph Han, Alexei A. Grom, Marco Gattorno, Angelo Ravelli, Fabrizio De Benedetti, Edward M. Behrens, Randy Q. Cron, Peter A. Nigrovic
ARTHRITIS & RHEUMATOLOGY
(2020)
Article
Multidisciplinary Sciences
Ruth J. Napier, Ellen J. Lee, Michael P. Davey, Emily E. Vance, Joao M. Furtado, Paige E. Snow, Kimberly A. Samson, Sydney J. Lashley, Brieanna R. Brown, Reiko Horai, Mary J. Mattapallil, Biying Xu, Michelle C. Callegan, Luke S. Uebelhoer, Christina L. Lancioni, Richard K. Vehe, Bryce A. Binstadt, Justine R. Smith, Rachel R. Caspi, Holly L. Rosenzweig
NATURE COMMUNICATIONS
(2020)
Editorial Material
Rheumatology
Daniel H. Solomon, Bryce A. Binstadt, David T. Felson, Peter A. Nigrovic
ARTHRITIS & RHEUMATOLOGY
(2021)
Review
Rheumatology
Bryce A. Binstadt, Peter A. Nigrovic
Summary: An unusual lung disease affecting children with systemic juvenile idiopathic arthritis is found to be associated with IL-1 and IL-6 antagonists. The disease mimics drug reaction with eosinophilia and systemic symptoms and may be triggered by an immune response to common microbes or other antigens, rather than the drugs themselves.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Rheumatology
Mark Gorelik, Sharon A. Chung, Kaveh Ardalan, Bryce A. Binstadt, Kevin Friedman, Kristen Hayward, Lisa F. Imundo, Sivia K. Lapidus, Susan Kim, Mary Beth Son, Sangeeta Sule, Adriana H. Tremoulet, Heather Van Mater, Cagri Yildirim-Toruner, Carol A. Langford, Mehrdad Maz, Andy Abril, Gordon Guyatt, Amy M. Archer, Doyt L. Conn, Kathy A. Full, Peter C. Grayson, Maria F. Ibarra, Peter A. Merkel, Rennie L. Rhee, Philip Seo, John H. Stone, Robert P. Sundel, Omar Vitobaldi, Ann Warner, Kevin Byram, Anisha B. Dua, Nedaa Husainat, Karen E. James, Mohamad Kalot, Yih Chang Lin, Jason M. Springer, Marat Turgunbaev, Alexandra Villa-Forte, Amy S. Turner, Reem A. Mustafa
Summary: This article summarizes expert recommendations for the diagnosis and management of Kawasaki disease. The recommendations are focused on clinical scenarios that rheumatologists commonly encounter when treating Kawasaki disease that is treatment-refractory, severe, or complicated.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Ben F. Brian, Monica L. Sauer, Joseph T. Greene, S. Erandika Senevirathne, Anders J. Lindstedt, Olivia L. Funk, Brian L. Ruis, Luis A. Ramirez, Jennifer L. Auger, Whitney L. Swanson, Myra G. Nunez, Branden S. Moriarity, Clifford A. Lowell, Bryce A. Binstadt, Tanya S. Freedman
Summary: This study reveals the distinct immunological functions of different splice variants of the Lyn kinase in vivo. LynB isoform exerts a dominant immunosuppressive function, while LynA isoform is uniquely required to restrain autoimmunity in female mice. Through gene editing, single-isoform LynA or LynB knockout mouse models were generated, showing isoform-specific and sexually dimorphic regulation of immune cell development and autoimmune diseases.
Article
Pediatrics
Meghan E. Ryan, Andrew Warmin, Bryce A. Binstadt, Colleen K. Correll, Emily Hause, Patricia Hobday, Alison Lerman, Shawn A. Mahmud, Mona M. Riskalla, Zachary Shaheen, Richard K. Vehe, Danielle R. Bullock
Summary: The study aimed to improve documentation of critical data elements by implementing quality improvement methods, and found that virtual visits had lower data capture rates compared to in-person visits.
PEDIATRIC RHEUMATOLOGY
(2022)
Article
Pediatrics
Shawn A. Mahmud, Danielle R. Bullock, Colleen K. Correll, Patricia M. Hobday, Mona M. Riskalla, Richard K. Vehe, Bryce A. Binstadt
Summary: Non-criteria antiphospholipid antibodies (NC-aPL) are associated with pediatric patients with antiphospholipid syndrome (APS), and doctors should consider the existence of NC-aPL when there is a high suspicion and routine antiphospholipid antibody tests are negative. Currently, there are no guidelines for NC-aPL in children or adults, so more data needs to be collected.
PEDIATRIC RHEUMATOLOGY
(2022)
Article
Rheumatology
Alison M. Lerman, Shawn A. Mahmud, Zineb Alfath, Benjamin W. Langworthy, Patricia M. Hobday, Mona M. Riskalla, Bryce A. Binstadt
Summary: Concerns arise over adverse drug reactions and lung disease (systemic JIA-LD) caused by medications, particularly interleukin (IL)-1 and IL-6 blocking agents, used in systemic juvenile idiopathic arthritis (JIA) treatment. The carriage of HLA-DRB1*15 has been identified as a risk factor for adverse drug reactions in systemic JIA patients. A retrospective chart review was conducted to evaluate these factors. Evaluation of adverse drug reactions and lung disease in systemic JIA patients is crucial for treatment and patient safety. Evaluation results confirm the association of HLA-DRB1*15 carriage with adverse drug reactions and the presence of systemic JIA-LD. Carriage of DRB1*15 was more common in the studied systemic JIA cohort. Eosinophilia and systemic JIA-LD were prevalent among severe systemic JIA cases with macrophage activation syndrome. The exposure to IL-1 and IL-6 blockers was high in patients. The findings emphasize the need for cautious monitoring and management of systemic JIA patients, especially those with HLA-DRB1*15 carriage. Rating: 8/10.
ARTHRITIS CARE & RESEARCH
(2023)
Article
Immunology
Matthew K. Mcelwee, Thamotharampillai Dileepan, Shawn A. Mahmud, Marc K. Jenkins
Summary: This study found that HLA-DR-bound citrullinated peptides are not neo-antigens and can induce varying degrees of immune tolerance. This may pose a barrier to rheumatoid arthritis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Immunology
Lee A. Meier, Jessica L. Faragher, Victoria Osinsk, Jennifer L. Auger, Rochus Voeller, Aubyn Marath, Bryce A. Binstadt
Summary: This study investigated the impact of the interaction between CD47-expressing apoptotic cells and signal regulatory protein a (SIRPa) on macrophages on disease progression in a model of autoimmune arthritis. The study found that blocking CD47 could prevent and treat valvular carditis, suggesting these molecules as potential therapeutic targets to reduce cardiovascular disease risk in autoantibody-driven inflammatory diseases.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Rheumatology
Meghan E. Ryan, Daniel Cortez, Kelly R. Dietz, Peter Karachunski, Bryce A. Binstadt
Summary: This case report presents a novel case of a juvenile idiopathic myositis patient with anti-MDA5 autoantibodies but lacking typical cutaneous and pulmonary manifestations associated with MDA5 positivity, instead presenting with cardiac conduction defects. This expands the clinical spectrum of MDA5-associated inflammatory myopathy.
