Journal
APPLIED SCIENCES-BASEL
Volume 9, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/app9020269
Keywords
flow control; culture dish adapter; small recess structure; closed environment; perfusion culture
Categories
Funding
- New Energy and Industrial Technology Development Organization (NEDO) of Japan [408005]
- Adaptable and Seamless Technology Transfer Program through Target-driven R&D (A-STEP) from the Japan Science and Technology Agency (JST) [AS2815006U]
- Leading Initiative for Excellent Young Researchers (LEADER) in FY 2016 of the Ministry of Education, Culture, Sports, Science and Technology, Japan
- [17K17715]
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Cell culture medium replacement is necessary to replenish nutrients and remove waste products, and perfusion and batch media exchange methods are available. The former can establish an environment similar to that in vivo, and microfluidic devices are frequently used. However, these methods are hampered by incompatibility with commercially available circular culture dishes and the difficulty in controlling liquid flow. Here, we fabricated a culture dish adapter using polydimethylsiloxane that has a small recess structure for flow control compatible with commercially available culture dishes. We designed U-shaped and I-shaped recess structure adapters and we examined the effects of groove structure on medium flow using simulation. We found that the U-shaped and I-shaped structures allowed a uniform and uneven flow of medium, respectively. We then applied these adaptors to 293T cell culture and examined the effects of recess structures on cell proliferation. As expected, cell proliferation was similar in each area of a dish in the U-shaped structure adapter, whereas in the early flow area in the I-shaped structure adapter, it was significantly higher. In summary, we succeeded in controlling liquid flow in culture dishes with the fabricated adapter, as well as in applying the modulation of culture medium flow to control cell culture.
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