Article
Immunology
Bo Yang, Ruicheng Yang, Bojie Xu, Jiyang Fu, Xinyi Qu, Liang Li, Menghong Dai, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: E. coli infection induced upregulation of miR-155 and miR-146a in astrocytes, which modulated bacteria-triggered neuroinflammatory responses through negative feedback regulation involving the TLR-mediated NF-kappa B and EGFR-NF-kappa B signaling pathways, thus protecting the central nervous system from further neuroinflammatory damage.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Chemistry, Medicinal
Ruicheng Yang, Yujin Lv, Ling Miao, Huipeng Zhang, Xinyi Qu, Jiaqi Chen, Bojie Xu, Bo Yang, Jiyang Fu, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: Resveratrol treatment has been shown to protect against CNS injury induced by meningitic E. coli by inhibiting bacterial invasion of the blood-brain barrier, reducing neuroinflammation, and improving survival rates. These findings suggest that resveratrol could be a potential new approach for preventing and treating E. coli meningitis in the future.
ACS INFECTIOUS DISEASES
(2021)
Article
Food Science & Technology
Gayani Weerasooriya, Andrea R. McWhorter, Samiullah Khan, Kapil K. Chousalkar
Summary: This study found that Campylobacter jejuni exhibited different response mechanisms to chlorine and acidified sodium chlorite (ASC), with ASC exposure inducing higher oxidative damage and cellular death. This suggests that ASC could be a potential effective sanitizer for use in the chicken meat industry.
NPJ SCIENCE OF FOOD
(2021)
Article
Neurosciences
Bo Yang, Bojie Xu, Ruicheng Yang, Jiyang Fu, Liang Li, Dong Huo, Jiaqi Chen, Xiaopei Yang, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: Research has shown that the lncRNA DDIT4-AS1 regulates the stability of DDIT4 mRNA in a RNA duplex formation, promoting neuroinflammatory responses caused by meningitic Escherichia coli. This discovery provides potential nucleic acid targets for the treatment of bacterial meningitis.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Microbiology
Nadeem Joudeh, Athanasios Saragliadis, Christian Schulz, Andre Voigt, Eivind Almaas, Dirk Linke
Summary: This study investigates the stress-related effects of Palladium (Pd) on E. coli, showing that exposure to Pd nanoparticles induces differential expression of genes, disrupting cellular homeostasis and affecting the expression of ion transporters and metabolism genes. The results suggest potential to genetically engineer E. coli strains with enhanced capabilities for Pd nanoparticles synthesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Ouyang Peng, Yu Xia, Ying Wei, Siying Zeng, Chuangchao Zou, Fangyu Hu, Qiuping Xu, Yihui Huang, Rui Geng, Guangli Hu, Yongchang Cao, Hao Zhang
Summary: This study utilized an in vivo approach to examine the comprehensive transcriptomic profiles of porcine alveolar macrophages during PRRSV infection. The results revealed the dynamic interplay among mRNAs, miRNAs, circRNAs, and lncRNAs, highlighting their involvement in immune-related pathways. Additionally, a competitive endogenous RNA (ceRNA) network was constructed, identifying potential therapeutic targets for PRRS.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Neurosciences
Bojie Xu, Ruicheng Yang, Bo Yang, Liang Li, Jiaqi Chen, Jiyang Fu, Xinyi Qu, Dong Huo, Chen Tan, Huanchun Chen, Zhong Peng, Xiangru Wang
Summary: This study uncovers the mechanism of lncRNA lncC11orf54-1 in meningitic E. coli-induced neuroinflammation by activating the NF-kappa B signaling pathway through interaction with IRAK1, leading to the production of pro-inflammatory cytokines.
Article
Neurosciences
Bojie Xu, Jiaqi Chen, Jiyang Fu, Ruicheng Yang, Bo Yang, Dong Huo, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: This study found that meningitic Escherichia coli induces the release of IL-17A, which leads to the degradation of tight junction and adherens junction proteins in human brain microvascular endothelial cells, increasing the permeability of the blood-brain barrier.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Environmental Sciences
Yan Fang, Wanfeng Wu, Yan Zhao, Haoqiang Liu, Zongda Li, Xinbo Li, Minwei Zhang, Yanan Qin
Summary: Graphene quantum dots (GQDs) are a novel broad-spectrum antibacterial agent that may be potential candidates in biomedical and food safety fields due to their outstanding antimicrobial properties and excellent biocompatibility. RNA-sequencing and LC-MS analysis were used to investigate the overall regulation of genes and metabolites in Escherichia coli (E. coli) after GQDs stimulation. The GQDs stimulation influenced gene transcription and metabolite expression related to crucial biomolecular processes including biofilm formation, bacterial secretion system, sulfur metabolism, and nitrogen metabolism. This study provides insights into the GQDs stress response in E. coli, which can be beneficial for the development and application of GQDs in food safety.
Article
Cell Biology
Kaili Li, Zuotian Huang, Chang Liu, Yuanyuan Xu, Wei Chen, Lu Shi, Can Li, Fawei Zhou, Fachun Zhou
Summary: This study used RNA-Seq technology to observe the gene expression in human pulmonary microvascular endothelial cells induced by LPS, and identified key genes and signaling pathways involved in ARDS. The study confirmed the role of TFDP1 in inflammation, apoptosis, and proliferation, as well as the involvement of SREBF1 in cell cycle arrest.
CELLULAR SIGNALLING
(2023)
Article
Genetics & Heredity
Xin Liu, Jing Zhang, Danyao Nie, Kun Zeng, Huiling Hu, Jinjun Tie, Liangnan Sun, Ling Peng, Xinhua Liu, Jiantao Wang
Summary: Transcriptome analysis of pterygium tissues identified differential expression of 579 mRNAs, 275 lncRNAs, and 21 circRNAs, which are associated with extracellular matrix organization, blood vessel morphogenesis, and focal adhesion. Key genes such as FN1, VCAM1, and MMP2, as well as lncRNAs like MIR4435-2HG and LINC00968, were identified to potentially play a role in the pathogenesis of pterygium. Additionally, circRNAs such as hsa_circ_0007482 and hsa_circ_001730 were found to be involved in the development of pterygium.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Zhiqiang Li, Liying Shi, Qianqing Li, Jing Zhao, Wenfa Lu, Jun Wang
Summary: This study investigated the role of circular RNAs in endometritis and identified their potential involvement in various signaling pathways related to disease progression. The findings provide new insights into the potential therapeutic applications of circular RNAs in the treatment of endometritis.
Article
Biology
Walja C. Wanney, Loubna Youssar, Gergana Kostova, Jens Georg
Summary: We provide evidence that transcriptional interference via the collision mechanism is a prevalent mechanism for bacterial gene regulation, based on stochastic simulations and experimental data. Rifampicin time-series data can be used to globally monitor and quantify collision between sense and antisense transcription-complexes. Our findings highlight the need to consider transcript segments with varying half-lives and transcriptional properties within a single gene annotation, and we introduce the 'rifi' R-package for accurately assessing RNA stability and detecting diverse transcriptional events.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Stephanie Bibert, Nicolas Guex, Joao Lourenco, Thomas Brahier, Matthaios Papadimitriou-Olivgeris, Lauro Damonti, Oriol Manuel, Robin Liechti, Lou Gotz, Jonathan Tschopp, Mathieu Quinodoz, Peter Vollenweider, Jean-Luc Pagani, Mauro Oddo, Olivier Hugli, Frederic Lamoth, Veronique Erard, Cathy Voide, Mauro Delorenzi, Nathalie Rufer, Fabio Candotti, Carlo Rivolta, Noemie Boillat-Blanco, Pierre-Yves Bochud
Summary: The dysregulated immunity in COVID-19 patients plays a key role in the severity of the disease, including defects in IFN responses, reduced toxicity-related molecules in NK cells, and increased neutrophil degranulation. Additionally, differences in gene expression patterns are also determinants of COVID-19 severity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ziqi Chen, Ruoyu Wang, Yinghong Zhu, Ziyao Huang, Xuewen Yang, Qiushi Li, Meijun Zhong, Wei Zhang, Lin Chen, Wei Wu, Li Feng, Ningbo An, Yi Yan
Summary: A novel circRNA named circSQSTM1 was identified to protect endothelial cells (ECs) in the progression of atherosclerosis. CircSQSTM1 relieved inflammation, inhibited oxidative stress, and promoted autophagy in ECs by upregulating Sirt1 through cytoplasmic and nuclear mechanisms. In a mouse model, overexpression of circSQSTM1 attenuated the progression of atherosclerosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)