Review
Cell Biology
Yuhan Zhang, Lingbo Sun, Changda Lei, Wenyan Li, Jiaqi Han, Jing Zhang, Yuecheng Zhang
Summary: This paper reviews the normal biosynthesis of mucin-type O-glycans and their role in maintaining body health. It also discusses the mechanisms of abnormal mucin-type O-glycosylation in disease development, especially cancer, with a specific focus on gastric cancer. Furthermore, tumor immunotherapy targeting mucin-type O-glycans is also discussed.
Article
Chemistry, Analytical
Mathieu Lamarre, Thomas Tremblay, Marc-Antoine Bansept, Karine Robitaille, Vincent Fradet, Denis Giguere, Denis Boudreau
Summary: In this study, a sensor immobilized with Tn antigen on a surface coated with polydopamine/polyethylene oxide showed high reliability and sensitivity in prostate cancer diagnostics. Detection limits of anti-Tn antibodies were validated in different concentration ranges and successfully used to discriminate blood serum samples from prostate cancer patients and cancer-free individuals.
Article
Chemistry, Multidisciplinary
Julia Koenig-Beihammer, Ulrike Vavra, Yun-Ji Shin, Christiane Veit, Clemens Gruenwald-Gruber, Yasmin Gillitschka, Jasmin Huber, Manuela Hofner, Klemens Vierlinger, Dieter Mitteregger, Andreas Weinhaeusel, Richard Strasser
Summary: Glycosylation plays an important role in the infectivity and immune evasion of viral envelope proteins. The heavily glycosylated spike protein of SARS-CoV-2 is influenced by host-derived glycan modifications, which impact immunogenic epitopes, virus-cell interaction, and virus transmission. In this study, recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) were produced with blood group antigens using an in vivo glycoengineering approach in Nicotiana benthamiana plants. The glycosylation variants with blood group antigens were shown to be functional and could potentially provide protection against virus infection in non-infected RBD-negative blood group O individuals.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Mingli Liu, Xianjin Qin, Xin-Shan Ye
Summary: Glycans have become a hot topic due to their biological activities, but their heterogeneity and complexity hinder research progress. Therefore, developing glycan assembly strategies to obtain structurally well-defined glycans is an important issue in carbohydrate chemistry.
Article
Cell Biology
Belinda L. Spillings, Christopher J. Day, Albert Garcia-Minambres, Anupriya Aggarwal, Nicholas D. Condon, Thomas Haselhorst, Damian F. J. Purcell, Stuart G. Turville, Jennifer L. Stow, Michael P. Jennings, Johnson Mak
Summary: This study reveals that HIV is captured near the lymphocyte surface in a virion-glycan-dependent manner. Removing certain glycans impairs virus-cell binding and interfering with glycan interactions reduces HIV infectivity. These findings provide new insights into the interaction between HIV and host cells.
Article
Biochemistry & Molecular Biology
Weidong Li, Kristof De Schutter, Els J. M. Van Damme, Guy Smagghe
Summary: Eukaryotic cells can modify their proteins with carbohydrate structures, affecting the proteins' properties. The study analyzed the O-glycan profile of different developmental stages of the red flour beetle using mass spectrometry technology, revealing predominance of mucin-type glycans. Interestingly, complex mucin-type O-glycans were abundant during the pupa stage, indicating a change in the insect O-glycan profile during metamorphosis.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Chemistry, Organic
Zhiyuan Chen, Guozhi Xiao
Summary: The highly branched tetradecasaccharide repeating unit and shorter sequences of GLSWA-1 from Ganoderma lucidum, which possess immune-enhancing activities, were prepared using a one-pot glycan assembly strategy that features orthogonal one-pot glycosylation, one-pot assembly of oligosaccharides, and modular and convergent [4+5+5] assembly of the highly branched tetradecasaccharide.
Article
Biochemistry & Molecular Biology
Collin J. Ballard, Miya R. Paserba, Earnest James Paul Daniel, Ramon Hurtado-Guerrero, Thomas A. Gerken
Summary: A large family of polypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts) play a crucial role in mucin O-glycosylation. The study reveals the importance of flanking charged residues in determining the substrate specificity of GalNAc-Ts. Different isoforms of GalNAc-Ts exhibit diverse charge specificities, and even isoforms with high sequence identity can have different charge preferences. These electrostatic interactions impact substrate binding and orientation on the enzyme surface, thus modulating their activities.
Review
Microbiology
Sabina Leanti La Rosa, Matthew P. Ostrowski, Arturo Vera-Ponce de Leon, Lauren S. McKee, Johan Larsbrink, Vincent G. Eijsink, Elisabeth C. Lowe, Eric C. Martens, Phillip B. Pope
Summary: This review summarizes recent insights into nutrient processing in microbiomes across different gastrointestinal ecosystems and discusses the implications for microbiome reprogramming to improve host health.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Andrew Bell, Nathalie Juge
Summary: The gut microbiota plays a critical role in human health, with alterations in its structure and function linked to various diseases. Mucus in the colon is essential for maintaining a balanced relationship with the gut microbiota, providing a safe environment for microbial communities. Commensal bacteria have evolved strategies to breakdown host mucin glycans, with a focus on glycoside hydrolases that aid in mucin degradation.
Article
Biochemistry & Molecular Biology
Reina F. Osuka, Masamichi Nagae, Akito Ohuchi, Shiho Ohno, Yoshiki Yamaguchi, Yasuhiko Kizuka
Summary: In this study, it was found through molecular dynamics simulations and biochemical experiments that residues outside the catalytic pocket of GnT-V are likely involved in the recognition of the core part of N-glycan. Additionally, the simulation suggested that UDP binding affects the orientation of the acceptor due to conformational changes. These findings provide new insights into how GnT-V recognizes its glycoprotein substrates.
Article
Chemistry, Analytical
Noortje de Haan, Yoshiki Narimatsu, Mikkel Koed Moller Aasted, Ida S. B. Larsen, Irina N. Marinova, Sally Dabelsteen, Sergey Y. Vakhrushev, Hans H. Wandall
Summary: In this study, a high-throughput sample preparation method was developed for the nonreductive release and characterization of O-glycans from human cell materials. By using reducing-end labeling and mass spectrometry analysis, O-glycan structural isomers in complex mixtures were efficiently separated and detected. This method provides an opportunity to study glycomic changes in human tissue and disease models using mainstream analytical equipment.
ANALYTICAL CHEMISTRY
(2022)
Review
Anatomy & Morphology
Kazuyoshi Itoh, Shoko Nishihara
Summary: Mucin-type O-glycosylation, a prominent type of glycosylation, is evolutionarily conserved and plays crucial physiological roles in animals. Studies in Drosophila have shown that these glycans are essential for CNS development, NMJ morphogenesis, and synaptic functions, providing insight into their functions in the mammalian brain.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Cell Biology
Ruisong Ding, Xingyou Hu, Wen Hu, Zhenzhen Du, Panpan Huang, Mengyang Wang, Jiaoyue Sheng, Yanchao Ma, Ailing Wang, Xiying Luan, Menghua Dong, Qizhi Cao, Yanfen Zou, Tao Hu
Summary: The study revealed that abnormal O-glycosylation caused by Cosmc mutations can lead to Tn antigen expression. Transfection of Cosmc resulted in reduced proliferation and migration of Tn(+) cells, along with increased sensitivity to apoptosis induced by Apo2L/TRAIL. Differences in O-glycan composition among various Tn(+) cells affect cell behavior and modulate sensitivity to apoptotic stimuli.
Article
Biochemistry & Molecular Biology
Brendon J. Medley, Leif Leclaire, Nicole Thompson, Keira E. Mahoney, Benjamin Pluvinage, Matthew A. H. Parson, John E. Burke, Stacy Malaker, Warren Wakarchuk, Alisdair B. Boraston
Summary: Akkermansia muciniphila, a key member of the human gut microbiota, has a significant impact on host health through its interaction with host mucin. This study identifies an uncharacterized protein, AMUC_1438, as a unique O-glycopeptidase with mucin-degrading capacity. The protein has a metzincin metalloprotease catalytic motif and specifically recognizes a GalNAc residue alpha-linked to serine or threonine. This understanding of the protein's structure and function contributes to the broader understanding of O-glycopeptidase enzymes and their role in host adaptation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Oncology
Imayavaramban Lakshmanan, Moorthy P. Ponnusamy, Muzafar A. Macha, Dhanya Haridas, Prabin Dhangada Majhi, Sukhwinder Kaur, Maneesh Jain, Surinder K. Batra, Apar Kishor Ganti
JOURNAL OF THORACIC ONCOLOGY
(2015)
Article
Oncology
Priya Pai, Satyanarayana Rachagani, Imayavaramban Lakshmanan, Muzafar A. Macha, Yuri Sheinin, Lynette M. Smith, Moorthy P. Ponnusamy, Surinder K. Batra
MOLECULAR ONCOLOGY
(2016)
Article
Oncology
Arokia Priyanka Vaz, Shonali Deb, Satyanarayana Rachagani, Parama Dey, Sakthivel Muniyan, Imayavaramban Lakshmanan, Saswati Karmakar, Lynette Smith, Sonny Johansson, Subodh Lele, Michel Ouellette, Moorthy P. Ponnusamy, Surinder K. Batra
Article
Oncology
Parthasarathy Seshacharyulu, Moorthy P. Ponnusamy, Satyanarayana Rachagani, Imayavaramban Lakshmanan, Dhanya Haridas, Ying Yan, Apar K. Ganti, Surinder K. Batra
Article
Oncology
Imayavaramban Lakshmanan, Parthasarathy Seshacharyulu, Dhanya Haridas, Satyanarayana Rachagani, Suprit Gupta, Suhasini Joshi, Chittibabu Guda, Ying Yan, Maneesh Jain, Apar K. Ganti, Moorthy P. Ponnusamy, Surinder K. Batra
Article
Oncology
Seema Chugh, Jane Meza, Yuri M. Sheinin, Moorthy P. Ponnusamy, Surinder K. Batra
BRITISH JOURNAL OF CANCER
(2016)
Article
Oncology
Ashley L. Hein, Parthasarathy Seshacharyulu, Satyanarayana Rachagani, Yuri M. Sheinin, Michel M. Ouellette, Moorthy P. Ponnusamy, Marc C. Mumby, Surinder K. Batra, Ying Yan
Article
Oncology
Suhasini Joshi, Eric Cruz, Satyanarayana Rachagani, Sushouan Guha, Randall E. Brand, Moorthy P. Ponnusamy, Sushil Kumar, Surinder K. Batra
MOLECULAR ONCOLOGY
(2016)
Article
Biochemistry & Molecular Biology
I. Lakshmanan, S. Rachagani, R. Hauke, S. R. Krishn, S. Paknikar, P. Seshacharyulu, S. Karmakar, R. K. Nimmakayala, G. Kaushik, S. L. Johansson, G. B. Carey, M. P. Ponnusamy, S. Kaur, S. K. Batra, A. K. Ganti
Article
Biochemistry & Molecular Biology
S. Joshi, S. Kumar, M. P. Ponnusamy, S. K. Batra
Article
Oncology
Saswati Karmakar, Parthasarathy Seshacharyulu, Imayavaramban Lakshmanan, Arokia P. Vaz, Seema Chugh, Yuri M. Sheinin, Sidharth Mahapatra, Surinder K. Batra, Moorthy P. Ponnusamy
Article
Biochemistry & Molecular Biology
S. Kumar, S. Das, S. Rachagani, S. Kaur, S. Joshi, S. L. Johansson, M. P. Ponnusamy, M. Jain, S. K. Batra
Article
Oncology
Shailendra K. Gautam, Ranjana K. Kanchan, Jawed A. Siddiqui, Shailendra K. Maurya, Sanchita Rauth, Naveenkumar Perumal, Pranita Atri, Ramakanth C. Venkata, Kavita Mallya, Sameer Mirza, Moorthy P. Ponnusamy, Vimla Band, Sidharth Mahapatra, Maneesh Jain, Surinder K. Batra, Mohd Wasim Nasser
Article
Oncology
Imayavaramban Lakshmanan, Shereen Salfity, Parthasarathy Seshacharyulu, Satyanarayana Rachagani, Abigail Thomas, Srustidhar Das, Prabin D. Majhi, Rama Krishna Nimmakayala, Raghupathy Vengoji, Subodh M. Lele, Moorthy P. Ponnusamy, Surinder K. Batra, Apar Kishor Ganti
CLINICAL CANCER RESEARCH
(2017)
