Article
Biochemistry & Molecular Biology
Eun-Hye Kim, Eun-Sun Kim, Donggeun Shin, Donghyun Kim, Sungbin Choi, Young-Jun Shin, Kyeong-A Kim, Dabi Noh, Ahmet B. Caglayan, G. K. Rajanikant, Arshad Majid, Ok-Nam Bae
Summary: The study found that carnosine inhibits MMP activity by chelating zinc, resulting in the reduction of edema and brain injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jeremy Tsung-Chieh Chen, Lea Schmidt, Christina Schuerger, Mohammed K. Hankir, Susanne M. Krug, Heike L. Rittner
Summary: The study revealed that chronic constriction injury in rats led to a reduction in netrin-1 protein and the netrin-1 receptor neogenin-1 in the sciatic nerve. Replacing netrin-1 through systemic or local administration alleviated injury-induced hypersensitivity. Mechanistically, netrin-1 restored endothelial and myelin barrier function via Neo1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vijay Arruri, Anil K. Chokkalla, Soomin Jeong, Bharath Chelluboina, Suresh L. Mehta, Krishna Kumar Veeravalli, Raghu Vemuganti
Summary: The increased expression of MMP-12 has been found to mediate blood-brain barrier disruption after stroke, while MMP-12 knockdown protects the brain and promotes functional recovery in mice.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Clinical Neurology
Shuang Wu, Lijun Song, Meirong Yu, Chao Gong, Lianhua Chen
Summary: Facial nerve injury leads to degradation of the neuromuscular junction and inhibits neurotransmission. Matrix metalloproteinases play critical roles in regulating NMJ remodeling. Treatment with the MMP inhibitor prinomastat after facial nerve injury can protect the function and morphology of the NMJ.
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tianhao Yu, Qiang Ao, Tianrang Ao, Muhammad Arslan Ahmad, Aijun Wang, Yingxi Xu, Zhongti Zhang, Qing Zhou
Summary: This study developed an optimized multichannel acellular nerve allograft (ANA) with improved ultrastructure and mechanical properties. The ANAs retained more extracellular matrix (ECM) components and bioactive molecules, which enhanced their regenerative capacity and biocompatibility. Both in vitro and in vivo results showed that the optimized ANAs had significant advantages in promoting nerve regeneration and functional recovery.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Chushan Zheng, Zehong Yang, Shihao Chen, Fang Zhang, Zilong Rao, Cailing Zhao, Daping Quan, Ying Bai, Jun Shen
Summary: NGCs composed of longitudinally aligned electrospun nanofibers and 0.25% pDNM gel show promising outcomes in directing axonal extension, promoting nerve fiber myelination, and facilitating functional recovery. Furthermore, T2-mapping and DTI metrics are found to be useful non-invasive monitoring techniques for PNI treatment.
Article
Biochemistry & Molecular Biology
Kana Yokoo, Yoshinari Yamamoto, Takuya Suzuki
Summary: Ammonia impairs TJ barrier in Caco-2 cells by increasing oxidative stress, leading to mitochondrial dysfunction, which may precede the ammonia-induced oxidative stress.
Article
Biochemistry & Molecular Biology
Devika P. P. Jayawardena, Marcello G. G. Masciantonio, Lefeng Wang, Sanjay Mehta, Natalie DeGurse, Cynthia Pape, Sean E. E. Gill
Summary: Sepsis is a life-threatening disease caused by excessive inflammation and organ dysfunction. The disruption of inter-cellular junctions in pulmonary microvascular endothelial cells (PMVEC) contributes to barrier dysfunction during sepsis. The imbalance between metalloproteinases and tissue inhibitors of metalloproteinases (TIMPs) is likely responsible for this barrier dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Meghali Nighot, Ashwinkumar Subramenium Ganapathy, Kushal Saha, Eric Suchanec, Eliseo F. Castillo, Alyssa Gregory, Steven Shapiro, Thomas Ma, Prashant Nighot
Summary: This study demonstrates the important role of MMP-12 in intestinal TJ barrier function and inflammation, showing that MMP-12(-/-) mice have increased tolerance to DSS colitis. Macrophage transmigration across the epithelial layer is MMP-12 dependent, highlighting MMP-12-mediated degradation of basement membrane laminin as a key pathogenic factor for intestinal inflammation.
JOURNAL OF CROHNS & COLITIS
(2021)
Article
Neurosciences
Leyang Li, Su Chen, Hiroyuki Yokoyama, Hidetoshi Kaburagi, Takashi Hirai, Kunikazu Tsuji, Mitsuhiro Enomoto, Yoshiaki Wakabayashi, Atsushi Okawa
Summary: Peripheral nerve injury (PNI) leads to severe functional loss in extremities, and delayed nerve repair results in muscle denervation and atrophy. To better understand NMJ degeneration and regeneration after PNI, we established two models of nerve repair in mice and compared their outcomes. Our results suggest that allogeneic nerve grafting is more effective than end-to-end neurorrhaphy in promoting functional recovery and increasing reinnervated NMJs and Schwann cells in the target muscle. Further investigation is needed to explore the relationship between NMJ and Schwann cells in the target muscle.
Review
Chemistry, Multidisciplinary
Junrong Wu, Zhenjun Zhu, Wenjing Liu, Yanli Zhang, Yiyuan Kang, Jia Liu, Chen Hu, Ruolan Wang, Manjin Zhang, Lili Chen, Longquan Shao
Summary: This article discusses the impacts of NPs on biological barriers' paracellular route and related mechanisms, providing a better understanding of how NPs can open the paracellular route. Additionally, it summarizes the limitations in existing research and outlines future research directions.
Article
Health Care Sciences & Services
Martin Aman, Kim S. Zimmermann, Mirjam Thielen, Benjamin Thomas, Simeon Daeschler, Arne H. Boecker, Annette Stolle, Amir K. Bigdeli, Ulrich Kneser, Leila Harhaus
Summary: This study comprehensively analyzed the epidemiological and etiological factors of peripheral nerve lesions in a large cohort. The findings highlight the importance of early diagnosis and treatment for early return to work and improved long-term functional outcomes.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Lan-Min Wang, Yu-Ting Wang, Wan-Xi Yang
Summary: This review summarizes the influences of four typical metal/metal oxide nanomaterials on the paracellular permeability of biological barriers, including disruptions on tight junctions, adhesion junctions, gap junctions, and desmosomes. Complex signaling pathways, as well as reactive oxygen species and cytokines, play crucial roles in ENP-triggered alterations in paracellular permeability.
Article
Pharmacology & Pharmacy
Shengyou Li, Beibei Yu, Xue Gao, Yi Zheng, Teng Ma, Yiming Hao, Haining Wu, Bin Wei, Yitao Wei, Zhuojing Luo, Bing Xia, Jinghui Huang
Summary: The study reveals that Trichostatin A (TSA) alleviates fibrosis by promoting M1 to M2 macrophage transition, thereby accelerating nerve regeneration and improving sensory functional recovery after nerve injury.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Lei Dai, Joseph Mugaanyi, Xingchen Cai, Mingjun Dong, Caide Lu, Changjiang Lu
Summary: This study comprehensively analyzed MMP1 in hepatocellular carcinoma and found that MMP1 was upregulated in patients with hepatocellular carcinoma and correlated with poor survival. A prognostic model was established and various correlations between MMP1 and immune-related cells/genes, as well as potential therapeutic agents, were identified.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Ruirui Zhang, Sailing Chen, Xinghui Wang, Xiaosong Gu, Sheng Yi
Summary: The study used high-coverage single-cell sequencing to reveal the cell diversity and heterogeneity of Schwann cells in the peripheral nerves of neonatal rats. A total of 10 cell types were identified in SN and DRG with distinct cell-cell communication patterns.
Article
Biochemistry & Molecular Biology
Yaxian Wang, Fuchao Zhang, Yunsong Zhang, Qi Shan, Wei Liu, Fengyuan Zhang, Feiyu Zhang, Sheng Yi
Summary: Research findings indicate that Btc plays essential roles in regulating Schwann cell migration and axon elongation, suggesting the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Qianqian Chen, Qianyan Liu, Yunsong Zhang, Shiying Li, Sheng Yi
Summary: The study reveals the important role of LIF in regulating the proliferation and migration of Schwann cells as well as the regeneration of injured peripheral nerves. Manipulation of LIF expression levels can affect Schwann cell behaviors and impact the process of peripheral nerve regeneration.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Ruirui Zhang, Qi Chen, Li Huang, Yunsong Zhang, Xinghui Wang, Sheng Yi
Summary: The study analyzed single-cell transcriptional profiling of Schwann cells in neonatal rat sciatic nerves, revealing cellular heterogeneity and dynamic changes. It also identified differentiation trajectory from certain Schwann cell subtypes to others, with functional interpretation showing genetic signatures of DNA replication and acquisition of mesenchymal traits in subtype 3 Schwann cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Neurosciences
Yinying Shen, Zhangchun Cheng, Sailing Chen, Yunsong Zhang, Qi Chen, Sheng Yi
Summary: miR-29a-3p is up-regulated in injured sciatic nerves and inhibits Schwann cell proliferation and migration by negatively regulating PMP22. Injection of miR-29a-3p agomir hinders Schwann cell activities, delays axon elongation and myelination, and retards the functional recovery of injured nerves. This highlights the important role of miR-29a-3p/PMP22 in regulating Schwann cell phenotype following sciatic nerve injury and peripheral nerve regeneration.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Yin-Ying Shen, Rui-Rui Zhang, Qian-Yan Liu, Shi-Ying Li, Sheng Yi
Summary: Cellular senescence and proliferation are essential for wound healing and tissue remodeling. This study analyzed gene expression patterns in rat sciatic nerve stumps and dorsal root ganglia after injury to reveal the genetic changes associated with cellular senescence and proliferation. The study found that cellular senescence and proliferation were less vigorous in the dorsal root ganglia compared to the sciatic nerve stumps.
NEURAL REGENERATION RESEARCH
(2022)
Article
Engineering, Multidisciplinary
Jian Yang, Lili Zhao, Sheng Yi, Fei Ding, Yumin Yang, Yan Liu, Yongjun Wang, Mei Liu, Chengbin Xue, Lian Xu, Leilei Gong, Xinghui Wang, Yu Zhang, Bin Yu, Guo-li Ming, Xiaosong Gu
Summary: The molecular network features of spinal cord development, including fluctuating RNA expression levels, microRNAs and transcriptional factors exhibiting mosaic profiles, and differential alternative splicing events, play crucial roles in initiating spinal cord patterning and development. Furthermore, there is a negative correlation between innate immunity and intrinsic growth capacity, with epigenetic modifications and G protein-coupled receptors contributing to the regulatory functions and pleiotropic roles in axonal growth. This study provides valuable insights for investigating spinal cord development and developing novel tissue engineering strategies.
Article
Biochemistry & Molecular Biology
Yunsong Zhang, Miao Yang, Yinying Shen, Sheng Yi, Xinghui Wang
Summary: This study found that MOGS is upregulated in nerve segments after peripheral nerve injury, and bioinformatic analysis indicated its involvement in cellular metabolism and negative regulation on Schwann cell plasticity and metabolic coupling with axons.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2022)
Article
Cell & Tissue Engineering
Xiaokun Gu, Sheng Yi, Aidong Deng, Hong Liu, Lai Xu, Jianhui Gu, Xiaosong Gu
Summary: Chitosan-PGLA nerve grafts combined with bone marrow mononuclear cells (BMMCs) can effectively repair long-distance peripheral nerve defects in the upper arm and achieve good clinical results. Sensory and motor functions gradually restored after surgery, allowing the patient to perform daily activities, and there was a significant increase in skin perfusion.
CURRENT STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Yunsong Zhang, Qian Zhao, Qianqian Chen, Lingchi Xu, Sheng Yi
Summary: Transcription factors play important roles in nerve regeneration after peripheral nerve injury, including regulating axon elongation and Schwann cell phenotype modulation. Understanding these transcription factors can help promote functional recovery of injured peripheral nerves.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Qian-Qian Chen, Qian-Yan Liu, Pan Wang, Tian-Mei Qian, Xing-Hui Wang, Sheng Yi, Shi-Ying Li
Summary: Neurotrophic factors, especially nerve growth factor, can enhance neuronal regeneration, but their in vivo applications are limited. This study investigated the potential of let-7 as a regulator in nerve repair, as it targets and regulates nerve growth factor. Anti-let-7a was identified as the most suitable let-7 family molecule, and its controlled delivery was achieved using a chitosan-hydrogel scaffold, promoting nerve regeneration and functional recovery in a rat model of sciatic nerve transection.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Qianqian Chen, Yang Miao, Zhipeng Xu, Ruirui Zhang, Sheng Yi
Summary: Schwann cells play a crucial role in nerve regeneration and are commonly used in tissue engineering. Identifying Schwann cells with enhanced migration ability is important for improving recovery effects. This study compared Schwann cells from different anatomical locations and found that dorsal root ganglia (DRG) Schwann cells have faster migration speed than sciatic nerve (SN) Schwann cells.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Neurosciences
Lili Zhao, Weixiao Huang, Sheng Yi
Summary: Single-cell RNA sequencing enables the classification and transcriptional profiling of individual cells, leading to a better understanding of cellular diversity. In the peripheral nervous system (PNS), this technique has identified multiple cell types including neurons, glial cells, ependymal cells, immune cells, and vascular cells. Subtypes of neurons and glial cells have also been recognized in different physiological and pathological states. This article summarizes the heterogeneity of cells in the PNS and describes cellular variability during development and regeneration, which provides insights into the complex cellular architecture of the PNS and serves as a basis for future genetic manipulation.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sailing Chen, Qianqian Chen, Xiaojiao Zhang, Yinying Shen, Xinyu Shi, Xiu Dai, Sheng Yi
Summary: Peripheral nerves have limited ability to regenerate after injury. The growth factor amphiregulin (AREG) is upregulated in Schwann cells of injured sciatic nerves and stimulates their proliferation and migration. Schwann cell-secreted AREG promotes neurite outgrowth and axon elongation. Administering AREG to injured nerves facilitates Schwann cell proliferation, cord formation, and axon regrowth. Overall, our study identifies AREG as an important neurotrophic factor and offers a promising therapeutic approach for peripheral nerve injuries.
JOURNAL OF NEUROCHEMISTRY
(2023)