Journal
FEBS OPEN BIO
Volume 9, Issue 7, Pages 1184-1193Publisher
WILEY
DOI: 10.1002/2211-5463.12574
Keywords
electrophysiology; heterologous expression; mitochondria; mitochondrial porins; planar lipid bilayer; VDAC2; yeast; yeastVDAC2; yVDAC2
Categories
Funding
- MIUR PRIN [2015795S5W_005]
- Piano della Ricerca UNICT 2017
- Fondazione Umberto Veronesi
Ask authors/readers for more resources
Voltage-dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae (yVDAC2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC2 and showed that it exhibits channel-forming activity in the planar lipid bilayer system when in its so-called native form. Here, we describe an alternative strategy for yVDAC2 isolation, through heterologous expression in bacteria and refolding in vitro. Recombinant yVDAC2, like its native form, is able to form voltage-dependent channels. However, some differences between native and recombinant yVDAC2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post-translational modification(s) that occur in eukaryotic cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available