Article
Microbiology
Yang He, Junfeng Zhao, Hua Yin, Yuan Deng
Summary: The study found that beer-spoilage yeast can be induced into a viable but non-culturable state by treatment with hop bitter acids, and can be recovered to a culturable state within a short period of time by the addition of catalase. Transcriptome profiling showed that certain genes were downregulated during the viable but non-culturable state, while others were upregulated during the recovery process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Zhenbo Xu, Kan Wang, Ziqi Liu, Thanapop Soteyome, Yang Deng, Ling Chen, Gamini Seneviratne, Wei Hong, Junyan Liu, Janette M. Harro, Birthe Kjellerup
Summary: This study confirmed that spoilage bacteria can enter the VBNC state and identified a case of beer spoilage caused by VBNC Pediococcus damnosus cells using a novel procedure. The combination of genomic sequencing, flow cytometry, and routine culturing can provide direct evidence of VBNC cells in spoiled food samples and aid in further research on food safety issues caused by VBNC microbes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Minami Umegatani, Nobuchika Takesue, Shizuka Asano, Hideyo Tadami, Kazuhiko Uemura
Summary: This study identified a beer spoilage strain Lactobacillus nagelii ABBC668 and discovered that it harbors hop resistance gene horA, exhibiting beer spoilage ability. This is the first report of L. nagelii as a beer spoilage species, with findings suggesting its unstable nature may contribute to its less frequent encounter in the brewing industry.
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2022)
Article
Food Science & Technology
Guiheng Yang, Cong Nie, Haojun Zhang, Shaokang Sun, Xiaochen Wang, Jie Zhang, Hengyuan Xu, Jinshang Liu
Summary: The study found that hexahydro iso-alpha-acids have significant antibacterial potency on E. coli and Lactococcus species, making them the most effective bacteriostat.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Le Zhao
Summary: A novel electrochemical immunosensor was fabricated for ultra-sensitive determination of Lactobacillus brevis, with gold nanoparticles electro-deposited on the electrode surface and ionic liquid used for improving the sensor's immobilization performance. After optimization, a linear regression equation was observed between the current increment and L. brevis concentration, with a detection limit estimated at 10^3 CFU/mL.
Article
Food Science & Technology
Aleksandra Modzelewska, Mateusz Jackowski, Anna Trusek
Summary: This study evaluated the impact of initial conditions and pitching methods on the mixed fermentation process and the characteristics of the final product. The results showed that the ethanol and lactic acid concentration of the final product were highly dependent on the initial conditions and pitching order. Interestingly, the presence of lactic acid bacteria did not significantly affect the final ethanol concentration. Optimization of conditions could improve the sour beer production process.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Aleksandra Modzelewska, Mateusz Jackowski, Anna Trusek
Summary: Mixed fermentation is a method used in sour beer production, and the properties of the final product rely heavily on the microorganisms pitched and the initial wort composition. This experiment evaluated the impact of different initial conditions and pitching methods on the mixed fermentation process and the final product's characteristics. Important observations were made, and optimal conditions for obtaining desired product qualities were calculated.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Engineering, Multidisciplinary
Heesun Yu, Jung Suk Kim, Dong Wook Kim, Eun Seok Park, Yu Seok Youn, Fakhar Ud Din, Jong Oh Kim, Sae Kwang Ku, Sung Giu Jin, Han-Gon Choi
Summary: This study aimed to develop a novel composite double-layered dressing (CDD) for an effective treatment of infected wounds and improved wound recovery. The LB-loaded hydrogel top layer was prepared using the freeze-thawing method, while the external layer of the dressing was produced using the high-temperature melting method, resulting in excellent mechanical properties and drug release. The developed LB-loaded CDD showed enhanced wound healing efficacy in infected wounds, making it a potential thermosensitive drug carrier for improving wound recovery.
COMPOSITES PART B-ENGINEERING
(2021)
Article
Food Science & Technology
Xiaoxue Chen, Xiaoning Huang, Shanfeng Sun, Beizhong Han
Summary: This study selected a Lactobacillus brevis strain with exceptional fermentation characteristics. Inoculating this strain strategically influenced the composition of microbial communities, improving the controllability of the fermentation process. Additionally, the metabolism of the inoculated strain promoted the formation of flavor compounds, leading to a reduction in fermentation time.
Article
Biotechnology & Applied Microbiology
Chunguang Luan, Weihua Cao, Na Luo, Jingxia Tu, Jianqin Hao, Yihong Bao, Feike Hao, Deliang Wang, Xin Jiang
Summary: In this study, the complete genome sequence of a novel beer-spoilage bacterium Lactobacillus acetotolerans CN247 was reported. The strain exhibited tolerance to acetic acid, ethanol, and streptomycin, and showed varying degrees of preference for different beer components. Genes associated with viable but non-culturable (VBNC) bacteria were also identified in the genome.
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
(2023)
Article
Food Science & Technology
Kriza Faye Calumba, Vondel Reyes, Franklin Bonilla, Elio Villasmil, Subramaniam Sathivel
Summary: This study utilized durian rind powder to immobilize probiotics in ale beer, resulting in extended viability and higher counts of probiotic cells compared to free cells. The findings suggest that ale beer could be a potential carrier for free and immobilized probiotic bacteria, demonstrating a novel application of probiotic lactic acid bacteria in beer brewing.
FOOD PRODUCTION PROCESSING AND NUTRITION
(2021)
Article
Fisheries
Azadeh Niazi, Mehdi Shamsaie Mehrgan, Houman Rajabi Islami
Summary: This study aimed to find the optimal fermentation technology of medicinal plants mixed with Lactobacillus brevis (FML) and to investigate its effects on the growth performance, immune response, and anti-microbial ability of rainbow trout fingerlings. The results showed that the optimized fermentation conditions were 8% probiotic and 25-hour fermentation time. The addition of FML to the fish diet significantly improved the growth indices, protease activity, immune function, and disease resistance of rainbow trout.
Article
Food Science & Technology
Anubhav Pratap-Singh, Andrew Suwardi, Ronit Mandal, Joana Pico, Simone D. D. Castellarin, David D. D. Kitts, Anika Singh
Summary: Pulsed light is a new non-thermal technology used to control microbial spoilage in foods and beverages. This study found that the UV portion of pulsed light can cause adverse sensory changes in beer by forming 3-MBT. The use of UV filters can reduce 3-MBT formation but also decrease microbial deactivation.
Article
Infectious Diseases
Ana C. Barros, Luis F. Melo, Ana Pereira
Summary: A proper assessment of the effects of biocides on bacterial cells is crucial for preventing antimicrobial resistance and implementing suitable biocidal programs. This study investigated how Pseudomonas fluorescens cells, previously exposed to different concentrations of benzalkonium chloride (BAC) and 2,2-Dibromo-3-nitrilopropionamide (DBNPA), behave upon restoration of optimum growth conditions. The results showed that cells labeled as 'dead' were able to recover to a greater extent, with cells exposed to higher BAC concentrations taking longer to rebuild their functional processes. The recovery of DBNPA-treated cells did not appear to be related to the biocide concentration. The study also reflects on the type of cells that were able to recover.
Article
Microbiology
Despoina Eugenia Kiousi, Joanna Bucka-Kolendo, Adrian Wojtczak, Barbara Sokolowska, Agapi I. I. Doulgeraki, Alex Galanis
Summary: This study presents the annotated whole genome sequence of two strains of Loigolactobacillus backii, a beer-spoiling bacteria with high hop tolerance. The genetic basis and adaptation of the strains in the brewery environment were studied, revealing their ability to survive and grow in the presence of hop compounds. The findings provide insights into the mechanisms behind the tolerance of Lg. backii to extreme conditions in the brewery.
Review
Microbiology
Zhenbo Xu, Zerong Lu, Thanapop Soteyome, Yanrui Ye, Tengyi Huang, Junyan Liu, Janette M. Harro, Birthe V. Kjellerup, Brian M. Peters
Summary: The coordination of microorganisms is necessary for traditional fermented foods. Lactobacillus and Saccharomyces cerevisiae are stable microbiotas, interacting through coexistence-relevant mechanisms. The review discusses metabolites communications, aggregation, and polymicrobial biofilm, as well as the molecular regulatory network of coexistence-relevant mechanisms based on omics research.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhenbo Xu, Ziqi Liu, Thanapop Soteyome, Jingjing Hua, Liang Zhang, Lei Yuan, Yanrui Ye, Zhao Cai, Liang Yang, Ling Chen, Janette M. Harro, Birthe Veno Kjellerup, Junyan Liu, Yanyan Li
Summary: The study investigated the transcriptional regulation of the Cronobacter sakazakii pmrA mutant during biofilm formation, revealing important genes involved in different stages of biofilm formation.
Article
Microbiology
Aifen Ou, Kan Wang, Yanrui Ye, Ling Chen, Xiangjun Gong, Lu Qian, Junyan Liu
Summary: A method using propidium monoazide-crossing priming amplification (PMA-CPA) was developed to detect Salmonella enterica rapidly with high sensitivity and identify VBNC cells in food samples. Specific primers and testing on 20 bacterial strains confirmed the effectiveness and specificity of this method in detecting S. enterica. The successful application of PMA-CPA in detecting S. enterica in VBNC state from food samples was demonstrated with significant color change.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Hua Jiang, Kan Wang, Muxia Yan, Qian Ye, Xiaojing Lin, Ling Chen, Yanrui Ye, Li Zhang, Junyan Liu, Tengyi Huang
Summary: This study developed a rapid and highly sensitive cross-priming amplification (CPA) method for detecting important toxins in MRSA, in combination with a rapid VBNC cell detection method. The PMA-CPA detection was able to identify various toxins in MRSA in the VBNC state with an accuracy rate of 100%.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Junyan Liu, Hubertine M. E. Willems, Emily A. Sansevere, Stefanie Allert, Katherine S. Barker, David J. Lowes, Andrew C. Dixson, Zhenbo Xu, Jian Miao, Christian DeJarnette, Helene Tournu, Glen E. Palmer, Jonathan P. Richardson, Francisco N. Barrera, Bernhard Hube, Julian R. Naglik, Brian M. Peters
Summary: Vulvovaginal candidiasis caused by Candida albicans poses significant issues for women globally. A conserved variant candidalysin isoform was identified across clinical isolates, with reduced virulence associated with impaired secretion. This study presents a novel mechanism for attenuating C. albicans virulence at the ECE1 locus.
Article
Food Science & Technology
Zhenbo Xu, Kan Wang, Ziqi Liu, Thanapop Soteyome, Yang Deng, Ling Chen, Gamini Seneviratne, Wei Hong, Junyan Liu, Janette M. Harro, Birthe Kjellerup
Summary: This study confirmed that spoilage bacteria can enter the VBNC state and identified a case of beer spoilage caused by VBNC Pediococcus damnosus cells using a novel procedure. The combination of genomic sequencing, flow cytometry, and routine culturing can provide direct evidence of VBNC cells in spoiled food samples and aid in further research on food safety issues caused by VBNC microbes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Zhenbo Xu, Tengyi Huang, Du Min, Thanapop Soteyome, Haifeng Lan, Wei Hong, Fang Peng, Xin Fu, Gongyong Peng, Junyan Liu, Birthe V. Kjellerup
Summary: Microorganisms exist in nature mainly in the form of biofilm, which poses a potential threat to public health safety. Among them, Candida albicans is an opportunistic pathogen causing severe infections with high mortality. This review aims to provide a comprehensive overview of the regulatory network controlling microbial biofilm development, with C. albicans as a representative, serving as a reference for therapeutic targets.
Review
Biotechnology & Applied Microbiology
Yan-Xia Liu, Yan-He Zhou, Chang-Hong Jiang, Junyan Liu, Ding-Qiang Chen
Summary: The COVID-19 pandemic is ongoing and countries such as China have been widely using herbal medicine for prevention and treatment with positive outcomes. However, the clinical and economic value of herbal medicine in COVID-19 treatment has not been fully evaluated.
Article
Biotechnology & Applied Microbiology
Junyan Liu, Xin Lin, Caiying Bai, Thanapop Soteyome, Xiaoxi Bai, Juexin Wang, Congxiu Ye, Xiaoyi Fan, Juzhen Liu, Yunzu Huang, Liyan Liu, Zhenbo Xu, Guangchao Yu, Birthe V. Kjellerup
Summary: Antimicrobial resistance is a major issue for global human health, threatening the achievement of sustainable development goals. Carbapenemase phenotype in Pseudomonas aeruginosa has been a major concern in antimicrobial resistance. This study found an obvious upward trend in the antimicrobial resistance identification rate of P. aeruginosa isolates from 2012 to 2016. By using a modified Carbapenem Inactivation Method, it was found that 18 out of 88 carbapenem-resistant P. aeruginosa strains were carbapenemase positive, which was consistent with traditional PCR results. This method shows potential in detecting carbapenemases producing P. aeruginosa.
Article
Biotechnology & Applied Microbiology
Junyan Liu, Xin Lin, Thanapop Soteyome, Yanrui Ye, Dingqiang Chen, Ling Yang, Zhenbo Xu
Summary: This study developed a multiplex amplification platform for rapid detection of beta-lactam resistance genes in clinical E. coli isolates. It was found that MDR isolates were commonly resistant to penicillins and fluoroquinolones, with higher distribution among females. Various plasmid replicons and beta-lactamase genes showed relatively high carriage rates in clinical E. coli.
Article
Microbiology
Junyan Liu, Amanda K. Vogel, Jian Miao, Jennifer A. Carnahan, David J. Lowes, Jeffrey M. Rybak, Brian M. Peters
Summary: Recently, the phenotypic heterogeneity in Candida albicans isolates has been recognized as an underappreciated factor contributing to gene diversification, antifungal resistance, fitness, and pathogenicity. We have developed a cloning-free genetic system that combines recyclable genetic systems with CRISPR-Cas9 technology for rapid gene deletion and reversion in C. albicans clinical isolates. By reengineering the SAT1-flipper plasmid to contain CaHygB gene encoding resistance to hygromycin B, both SAT1- and CaHygB-flipper cassettes were integrated at loci with high efficiency and homozygous gene targeting exceeding 99% across various anatomical isolates. Revertant strains were constructed by targeted integration at the NEUT5 locus. This system will aid in understanding the genetic factors contributing to intraspecies diversity.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Junyan Liu, Brian M. Peters, Ling Yang, Hui Yu, Donghua Feng, Dingqiang Chen, Zhenbo Xu
Summary: In this study, a distinct clone of Ralstonia mannitolilytica was identified as a colonizer for the first 20 days during hospitalization and then confirmed as a causative agent for catheter-related bloodstream infection after effective treatment. This suggests that R. mannitolilytica transitioned from a commensal to a pathogen in the respiratory tract during the patient's hospitalization.
Review
Biochemistry & Molecular Biology
Junyan Liu, Liang Yang, Birthe Veno Kjellerup, Zhenbo Xu
Summary: This article discusses the importance and controversies surrounding the viable but nonculturable (VBNC) state, and proposes the need to redefine and standardize the term and accurately determine VBNC cells.
TRENDS IN MICROBIOLOGY
(2023)
Editorial Material
Infectious Diseases
Junyan Liu, Zhenbo Xu, Yulong Tan, Ren-You Gan, Guanggang Qu, Dingqiang Chen
Article
Infectious Diseases
Junyan Liu, Tengyi Huang, Thanapop Soteyome, Jian Miao, Guangchao Yu, Dingqiang Chen, Congxiu Ye, Ling Yang, Zhenbo Xu
Summary: In this study, a large-scale investigation was conducted on nosocomial Staphylococcus aureus strains in Southern China from 2009 to 2015. The study focused on antibiotic resistance, virulence gene acquisition, and molecular types. The findings revealed an increase in MRSA resistance, significant differences in virulence genes, and molecular types. Importantly, this study fills a gap in previous research since 2006 or 2010.
