Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures

Total protein or high-abundance protein: Which offers the best loading control for Western blotting?

(2016) Jonathan S. Thacker et al.   ANALYTICAL BIOCHEMISTRY

A Proteomics Approach to the Protein Normalization Problem: Selection of Unvarying Proteins for MS-Based Proteomics and Western Blotting

(2016) Jacek R. Wiśniewski et al.   JOURNAL OF PROTEOME RESEARCH

Considerations when quantitating protein abundance by immunoblot

(2015) Alicia A. McDonough et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Cy5 total protein normalization in Western blot analysis

(2015) Åsa Hagner-McWhirter et al.   ANALYTICAL BIOCHEMISTRY

Reproducibility and cell biology

(2015) Kenneth M. Yamada et al.   JOURNAL OF CELL BIOLOGY

Reproducibility crisis: Blame it on the antibodies

(2015) Monya Baker   NATURE

Reproducibility: Standardize antibodies used in research

(2015) Andrew Bradbury et al.   NATURE

An analysis of critical factors for quantitative immunoblotting

(2015) Kevin A. Janes   Science Signaling

An analysis of critical factors for quantitative immunoblotting

(2015) Kevin A. Janes   Science Signaling

Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting

(2014) B. Rivero-Gutiérrez et al.   ANALYTICAL BIOCHEMISTRY

The necessity of and strategies for improving confidence in the accuracy of western blots

(2014) Rajeshwary Ghosh et al.   Expert Review of Proteomics

Editorial: Antibody Can Get It Right: Confronting Problems of Antibody Specificity and Irreproducibility

(2014) Agnes Schonbrunn   MOLECULAR ENDOCRINOLOGY

Evaluating Strategies to Normalise Biological Replicates of Western Blot Data

(2014) Andrea Degasperi et al.   PLoS One

The Design of a Quantitative Western Blot Experiment

(2014) Sean C. Taylor et al.   Biomed Research International

Stain-Free total protein staining is a superior loading control to β-actin for Western blots

(2013) Jennifer E. Gilda et al.   ANALYTICAL BIOCHEMISTRY

Direct Blue 71 staining as a destaining-free alternative loading control method for Western blotting

(2013) Li Zeng et al.   ELECTROPHORESIS

Important considerations for protein analyses using antibody based techniques: down-sizing Western blotting up-sizes outcomes

(2013) Robyn M. Murphy et al.   JOURNAL OF PHYSIOLOGY-LONDON

A Defined Methodology for Reliable Quantification of Western Blot Data

(2013) Sean C. Taylor et al.   MOLECULAR BIOTECHNOLOGY

Total Protein Analysis as a Reliable Loading Control for Quantitative Fluorescent Western Blotting

(2013) Samantha L. Eaton et al.   PLoS One

Decreased myometrial p160 ROCK-1 expression in obese women at term pregnancy

(2013) Margaret O’Brien et al.   Reproductive Biology and Endocrinology

Comparison of Stain-Free gels with traditional immunoblot loading control methodology

(2012) Alex D. Colella et al.   ANALYTICAL BIOCHEMISTRY

Using soybean trypsin inhibitor as an external loading control for Western blot analysis of tear proteins: Application to corneal disease

(2012) Jingjing You et al.   EXPERIMENTAL EYE RESEARCH

Stability of reference proteins in human placenta: General protein stains are the benchmark

(2012) D. Lanoix et al.   PLACENTA

Avoiding Pitfalls of Internal Controls: Validation of Reference Genes for Analysis by qRT-PCR and Western Blot throughout Rat Retinal Development

(2012) Maurício Rocha-Martins et al.   PLoS One

Selection of reference genes for gene expression studies in ultraviolet B-irradiated human skin fibroblasts using quantitative real-time PCR

(2011) Li Li et al.   BMC MOLECULAR BIOLOGY

Use of Sample Mixtures for Standard Curve Creation in Quantitative Western Blots

(2011) Osamu SUZUKI et al.   EXPERIMENTAL ANIMALS

Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1)

(2011) C. A. Hudson et al.   MOLECULAR HUMAN REPRODUCTION

Phasic Phosphorylation of Caldesmon and ERK 1/2 during Contractions in Human Myometrium

(2011) Jonathan Paul et al.   PLoS One

Reversible Ponceau staining as a loading control alternative to actin in Western blots

(2010) Isabel Romero-Calvo et al.   ANALYTICAL BIOCHEMISTRY

Housekeeping genes; expression levels may change with density of cultured cells

(2010) Samantha Greer et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Coomassie Staining as Loading Control in Western Blot Analysis

(2010) Charlotte Welinder et al.   JOURNAL OF PROTEOME RESEARCH

Quantification of Rapid Myosin Regulatory Light Chain Phosphorylation Using High-Throughput In-Cell Western Assays: Comparison to Western Immunoblots

(2010) Hector N. Aguilar et al.   PLoS One

Are genuine changes in protein expression being overlooked? Reassessing Western blotting

(2009) Janelle P. Mollica et al.   ANALYTICAL BIOCHEMISTRY

All data are not created equal

(2009) Ushma S. Neill   JOURNAL OF CLINICAL INVESTIGATION

Improved semiquantitative Western blot technique with increased quantification range

(2009) F. Heidebrecht et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Stretch Activates Human Myometrium via ERK, Caldesmon and Focal Adhesion Signaling

(2009) Yunping Li et al.   PLoS One

The use of total protein stains as loading controls: An alternative to high-abundance single-protein controls in semi-quantitative immunoblotting

(2008) Georgina M. Aldridge et al.   JOURNAL OF NEUROSCIENCE METHODS

Contraction in Human Myometrium Is Associated with Changes in Small Heat Shock Proteins

(2007) David A. MacIntyre et al.   ENDOCRINOLOGY