Article
Physiology
Irene Ramos-Alvarez, Lingaku Lee, Robert T. Jensen
Summary: This study demonstrates that CCK can activate cofilin to promote enzyme secretion and MAPK activation in pancreatic acinar cells, indicating its important role in insulin secretion, pancreatic cancer cell growth, and pancreatitis.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cell Biology
Mahesh Kathania, Ritesh Kumar, Elviche Taskem Lenou, Venkatesha Basrur, Arianne L. Theiss, Jonathan Chernoff, K. Venuprasad
Summary: Dysregulated interleukin-17 expression and its downstream signaling is strongly linked to inflammatory bowel diseases. We identified that Pak2 directly interacts with ROR gamma t and regulates its activity through phosphorylation.
Article
Biotechnology & Applied Microbiology
Yin-Hao Shi, Qiong-Cong Xu, Ying-Qin Zhu, Zhi-De Liu, Guang-Yin Zhao, Qi Liu, Xi-Yu Wang, Jie-Qin Wang, Xiang Xu, Qiao Su, Jia-Ming Lai, Chen-Song Huang, Xiao-Yu Yin
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a malignant tumor with poor prognosis. Gemcitabine-based chemotherapy is one of the main treatment modalities, but gemcitabine resistance frequently occurs. Platelet-derived growth factor C (PDGFC) is abnormally highly expressed in gemcitabine-resistant PDAC. Silencing PDGFC expression enhances the therapeutic effect of gemcitabine on PDAC. PDGFC promotes gemcitabine resistance by activating the PDGFR-PI3K-AKT signaling pathway.
Article
Multidisciplinary Sciences
Fenghua Bian, Ying-Wei Lan, Shuyang Zhao, Zicheng Deng, Samriddhi Shukla, Anusha Acharya, Johnny Donovan, Tien Le, David Milewski, Matthew Bacchetta, Ahmed Emad Hozain, Yuliya Tipograf, Ya-Wen Chen, Yan Xu, Donglu Shi, Vladimir V. V. Kalinichenko, Tanya V. V. Kalin
Summary: This study reveals the inhibitory role of endothelial cells in pulmonary fibrosis, and shows that endothelial-specific delivery of FOXF1 gene therapy reduces lung fibrosis in mice.
NATURE COMMUNICATIONS
(2023)
Article
Endocrinology & Metabolism
Selina Wrublewsky, Thimoteus Speer, Lisa Nalbach, Anne S. Boewe, Mandy Pack, Dalia Alansary, Leticia P. Roma, Markus D. A. Hoffmann, Beate M. Schmitt, Andrea Weinzierl, Michael D. Menger, Matthias W. Laschke, Emmanuel Ampofo
Summary: The inhibition of NLRP3 improves the survival and endocrine function of pancreatic islet grafts by enhancing revascularization and inducing insulin gene expression. This inhibition has significant therapeutic potential in pancreatic islet transplantation.
Article
Cell Biology
Juan Zeng, Shiwei Wang, Min Gao, Dian Lu, Shuang Song, Diyu Chen, Weimin Fan, Zhiliang Xu, Zhiguo Zhang, Xiaofang Sun
Summary: As a highly conserved and ubiquitously expressed kinase, PAK2 plays important roles in meiotic progression and chromosome alignment in mouse oocytes. Depletion of PAK2 leads to metaphase I arrest and meiotic chromosome alignment defects, partly due to the reduction of PLK1. The interaction between PAK2 and PLK1 protects PLK1 from degradation and promotes meiotic progression and bipolar spindle formation.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Yan Zou, Le Pan, Yi Shen, Xiang Wang, Chenxing Huang, Hao Wang, Xuejuan Jin, Chao Yin, Ying Wang, Jianguo Jia, Juying Qian, Yunzeng Zou, Hui Gong, Junbo Ge
Summary: Progressive cardiac fibrosis is accelerated by elevated serum levels of Wnt5a and Wnt11 in hypertension patients, leading to diastolic dysfunction. Pressure overload increases the secretion of Wnt5a and Wnt11 from cardiomyocytes and cardiac fibroblasts, promoting cardiac fibrosis through the activation of FZD5 and EGFR crosstalk.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Xianglian Zhang, Ya Luo, Yu Cen, Xin Qiu, Jing Li, Mengmeng Jie, Shiming Yang, Shanyu Qin
Summary: MACC1 is found to promote metastasis in pancreatic cancer and is associated with poor prognosis. By binding to SNAI1, MACC1 drives the mesenchymal transition of pancreatic cancer cells, facilitating metastasis. This discovery reveals a new mechanism and suggests that the MACC1-SNAI1 complex-mediated mesenchymal transition may serve as a therapeutic target in pancreatic cancer.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Linli Qi, Xiaojing Li, Fang Zhang, Xingguo Zhu, Qi Zhao, Dan Yang, Shujie Hao, Tong Li, Xiangyue Li, Taikun Tian, Jian Feng, Xiaochen Sun, Xilin Wang, Shangyan Gao, Hanzhong Wang, Jing Ye, Shengbo Cao, Yulong He, Hongyan Wang, Bin Wei
Summary: Using murine models, we found that vascular endothelial growth factor receptor 3 (VEGFR-3) is upregulated in macrophages in the central nervous system (CNS) upon viral infection, while infected neurons secrete the ligand VEGF-C. VEGFR-3(+) macrophages, when cultured with VEGF-C-containing supernatants from infected neurons, suppress tumor necrosis factor alpha (TNF-α) secretion to reduce neuron apoptosis. In mice treated with the VEGFR-3 kinase inhibitor or lacking the ligand-binding domain in myeloid cells, the severity of encephalitis, TNF-α production, and neuron apoptosis post Japanese encephalitis virus (JEV) infection worsens. Activating VEGFR-3 or blocking TNF-α can alleviate encephalitis and neuronal damage upon JEV infection.
Article
Multidisciplinary Sciences
Meilin Xue, Youwei Zhu, Yongsheng Jiang, Lijie Han, Minmin Shi, Rui Su, Liwen Wang, Cheng Xiong, Chaofu Wang, Ting Wang, Shijie Deng, Dong Wu, Yizhi Cao, Lei Dong, Fan Bai, Shulin Zhao, Xiaxing Deng, Chenghong Peng, Hongwei Li, Jianjun Chen, Baiyong Shen, Lingxi Jiang, Hao Chen
Summary: Schwann cells induce malignant subtypes of tumor cells and cancer-associated fibroblasts (CAFs) in the PDAC milieu. They enhance the proliferation and migration of PDAC cells via Midkine signaling and promote the switch of CAFs to inflammatory CAFs via interleukin-1α.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Nicole K. Patel, Johanna H. Nunez, Michael Sorkin, Simone Marini, Chase A. Pagani, Amy L. Strong, Charles D. Hwang, Shuli Li, Karthik R. Padmanabhan, Ravi Kumar, Alec C. Bancroft, Joey A. Greenstein, Reagan Nelson, Husain A. Rasheed, Nicholas Livingston, Kaetlin Vasquez, Amanda K. Huber, Benjamin Levi
Summary: The study demonstrates the critical role of autocrine TGF-beta 1 signaling in macrophages in heterotopic ossification (HO) after traumatic injury. The findings highlight the importance of the TGF-beta 1/ALK5 signaling pathway in HO formation.
Article
Biochemistry & Molecular Biology
Hong-Yong Cui, Shi-Jie Wang, Fei Song, Xu Cheng, Gang Nan, Yu Zhao, Mei-Rui Qian, Xi Chen, Jia-Yue Li, Fen-Ling Liu, Yu-Meng Zhu, Ruo-Fei Tian, Bin Wang, Bin Wu, Yang Zhang, Xiu-Xuan Sun, Ting Guo, Xiang-Min Yang, Hai Zhang, Ling Li, Jing Xu, Hui-Jie Bian, Jian-Li Jiang, Zhi-Nan Chen
Summary: Major gaps in understanding the molecular mechanisms of colorectal cancer progression and mucosal repair have hindered therapeutic development for gastrointestinal disorders. TFF3 plays a crucial role in CRC progression and mucosal repair, with upregulated TFF3 predicting worse survival rates in CRC patients. The interaction between TFF3 and CD147 enhances CRC progression by activating STAT3 and PTGS2, leading to increased migration, proliferation, and invasion of cancer cells.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Huang Chen, Aiwu Bian, Lian-fang Yang, Xuan Yin, Jie Wang, Chaowen Ti, Ying Miao, Shihong Peng, Shifen Xu, Mingyao Liu, Wen-Wei Qiu, Zhengfang Yi
Summary: A new small-molecule inhibitor N4 shows potent anti-tumor bioactivity in pancreatic cancer by targeting STAT3, effectively suppressing tumor growth and metastasis, and potentially offering clinical benefits for treatment.
Article
Medicine, Research & Experimental
Wenyu Fu, Wenhuo Hu, Young-Su Yi, Aubryanna Hettinghouse, Guodong Sun, Yufei Bi, Wenjun He, Lei Zhang, Guanmin Gao, Jody Liu, Kazuhito Toyo-oka, Guozhi Xiao, David B. Solit, Png Loke, Chuan-ju Liu
Summary: This study identified 14-3-3c as a crucial component of the TNFR2 receptor complex, playing a key role in regulating macrophage polarization and anti-inflammatory effects. Deletion of 14-3-3c exacerbated inflammatory arthritis and counteracted the protective effects of TNFR2 activation against inflammation and autoimmunity. TNFR2/14-3-3c signaling pathway restricts NF-KB activation while stimulating C/EBPp activation, influencing macrophage plasticity.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Anjiang Zhao, Dan Li, Xiongmin Mao, Mengliu Yang, Wuquan Deng, Wenjing Hu, Chen Chen, Gangyi Yang, Ling Li
Summary: This study investigates the role of G-protein gamma subunit 2 (GNG2) in breast cancer. The study identifies GNG2 as one of the differentially expressed genes in breast cancer tissues and shows that its expression is reduced in all subtypes of breast cancer. Further experiments demonstrate that GNG2 over-expression inhibits cell cycle and proliferation, and promotes apoptosis in breast cancer cells. Animal studies also reveal that GNG2 over-expression inhibits the growth of breast cancer cells. The study suggests that GNG2 interacts with muscle RAS oncogene homolog (MRAS) to inhibit the activity of ERK and Akt, leading to apoptosis and suppression of proliferation in breast cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)