Article
Geriatrics & Gerontology
Patricia Sosa, Elena Alcalde-Estevez, Ana Asenjo-Bueno, Patricia Plaza, Natalia Carrillo-Lopez, Gemma Olmos, Susana Lopez-Ongil, Maria Piedad Ruiz-Torres
Summary: Hyperphosphatemia impairs myogenic differentiation and leads to muscle fibrosis, with studies in older mice demonstrating a close relationship between age-related hyperphosphatemia and the decrease in myogenic factors and increase in fibrotic factors.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Nasa Xu, Jianbo Wu, Jose L. Ortiz-Vitali, Yong Li, Radbod Darabi
Summary: Recent advancements in reprogramming somatic cells into induced pluripotent stem cells (iPSCs) have allowed for the efficient derivation of skeletal myogenic progenitors from human pluripotent stem cells (ESCs/iPSCs) using a directed differentiation method without the need for gene integration or modification. This method induces mesoderm and subsequently myotomal progenitors, enabling the generation of skeletal myogenic progenitors in a short time frame with high efficiency.
Article
Biochemistry & Molecular Biology
Aiping Lu, Ping Guo, Haiying Pan, Chieh Tseng, Krishna M. Sinha, Fan Yang, Alex Scibetta, Yan Cui, Matthieu Huard, Ling Zhong, Sudheer Ravuri, Johnny Huard
Summary: Research shows that pregnant mice demonstrate accelerated muscle healing, superior muscle regeneration, and reduced inflammation and necrosis following muscle injury. Additionally, MPCs from pregnant mice display improved myogenic differentiation capacity in vitro and muscle regeneration in vivo.
Article
Biology
Shufang Cai, Bin Hu, Xiaoyu Wang, Tongni Liu, Zhuhu Lin, Xian Tong, Rong Xu, Meilin Chen, Tianqi Duo, Qi Zhu, Ziyun Liang, Enru Li, Yaosheng Chen, Jianhao Li, Xiaohong Liu, Delin Mo
Summary: In this study, the molecular and cellular dynamics of pig embryonic myogenesis were studied through single-cell resolution analysis of gene expression and chromatin accessibility. The differentiation trajectory of pig skeletal muscle development was identified, and critical regulators such as EGR1 and RHOB were identified through integrative analysis.
Article
Biochemistry & Molecular Biology
Flavia Carton, Dalila Di Francesco, Luca Fusaro, Emma Zanella, Claudio Apostolo, Francesca Oltolina, Diego Cotella, Maria Prat, Francesca Boccafoschi
Summary: The study evaluated the myogenic potential of dECM substrate derived from decellularized bovine pericardium on murine muscle cells and found that dECM could support and enhance the myogenic potential of muscle cells compared to cells grown on a standard plastic surface. This suggests that extracellular matrix proteins could be a promising therapeutic strategy for skeletal muscle tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Qian Li, Yahui Wang, Xin Hu, Yapeng Zhang, Hongwei Li, Qi Zhang, Wentao Cai, Zezhao Wang, Bo Zhu, Lingyang Xu, Xue Gao, Yan Chen, Huijiang Gao, Junya Li, Lupei Zhang
Summary: This study investigates the epigenetic changes and gene expression patterns during the development of skeletal muscle in bovine fetuses. Through in vitro culturing and induction, the researchers observed the dynamic changes of chromatin accessibility and gene expression at different stages of myoblast differentiation. The study also identified key transcriptional regulators and cis-regulatory regions involved in bovine skeletal muscle development. By combining the findings with genomic data, the researchers provide insights for a more accurate annotation of genetic variants related to bovine growth traits.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Clara Crescioli
Summary: Skeletal muscle cells are finely controlled by vitamin D through genomic and non-genomic mechanisms, constantly undergoing cell remodeling to maintain tissue integrity. Vitamin D insufficiency promotes cell maladaptation, while restoring vitamin D levels can enhance myogenic programming.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Li-Ting Diao, Shu-Juan Xie, Hang Lei, Xiu-Sheng Qiu, Meng-Chun Huang, Shuang Tao, Ya-Rui Hou, Yan-Xia Hu, Yu-Jia Sun, Qi Zhang, Zhen-Dong Xiao
Summary: METTL3 increases mature miRNA levels by modifying pri-miRNA transcripts through m6A, but surprisingly represses muscle-specific miRNAs in C2C12 cells and mouse skeletal muscle regeneration models. This repression may involve both transcriptional and post-transcriptional regulations.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Developmental Biology
Olga G. Jaime, Jessica Arias, Shreya Pavani, April D. Pyle, Michael R. Hicks
Summary: The co-expression of SIX1 and PAX3 is the most indicative factor for skeletal muscle differentiation in human pluripotent stem cells (hPSCs). Manipulating seeding density, monitoring metabolic secretion, and altering the concentration of CHIR99021 can enhance the emergence of SIX1+PAX3+ precursors in hPSC differentiation. Inhibition of non-myogenic lineages can modulate PAX3 expression independently of SIX1.
Article
Chemistry, Multidisciplinary
Inseon Kim, Seunghun S. Lee, Adhideb Ghosh, Stephen J. Ferguson, Ori Bar-Nur
Summary: This study presents an engineered biomimetic muscle construct that can self-regenerate and produce aligned myotubes using induced myogenic progenitor cells. The construct faithfully recapitulates a step-wise myogenic differentiation program and can rapidly differentiate into aligned myotubes within the constructs when subjected to a damaging myonecrotic agent. This work showcases the potential for translational applicability in regenerative medicine.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Article
Biochemistry & Molecular Biology
Mamoru Oyabu, Kaho Takigawa, Sako Mizutani, Yukino Hatazawa, Mariko Fujita, Yuto Ohira, Takumi Sugimoto, Osamu Suzuki, Kyoichiro Tsuchiya, Takayoshi Suganami, Yoshihiro Ogawa, Kengo Ishihara, Shinji Miura, Yasutomi Kamei
Summary: This study identifies the FOXO-CCAAT/enhancer-binding protein delta (C/EBP delta) signaling pathway as a novel proteolytic pathway. By comparing the gene expression profiles of transgenic mice and loss-of-function mice, they identified several new FOXO1 target genes in skeletal muscle. The study also found that FOXO1 enhances the promoter activity of target genes in cooperation with C/EBP delta and ATF4.
Article
Biochemistry & Molecular Biology
Denis Vecellio Reane, Cristina Cerqua, Sabrina Sacconi, Leonardo Salviati, Eva Trevisson, Anna Raffaello
Summary: Alternative splicing plays a crucial role in skeletal muscle, both during myogenesis and in post-natal life, by regulating the transcripts of contractile proteins, metabolic enzymes, and transcription factors. Aberrations in alternative splicing can lead to pathological conditions. In addition, many skeletal muscle Ca2+ homeostasis genes are also regulated by alternative splicing mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Dan Li, Rong Shao, Na Wang, Nan Zhou, Kaili Du, Jiahui Shi, Yihan Wang, Zhuangzhuang Zhao, Xin Ye, Xiaoli Zhang, Haoxing Xu
Summary: Oxidative stress is implicated in various pathological conditions, with antioxidant-rich foods playing a role in maintaining cellular redox balance. Sulforaphane, enriched in cruciferous vegetables like broccoli, induces cellular antioxidant responses by activating TFEB. This activation helps in protecting cells against oxidative stress through a combination of autophagy and detoxification pathways.
Article
Biochemistry & Molecular Biology
Maria Paula Zappia, Lucia de Castro, Majd M. Ariss, Holly Jefferson, Abul B. M. M. K. Islam, Maxim Frolov
Article
Cell Biology
Majd M. Ariss, Alexander R. Terry, Abul B. M. M. K. Islam, Nissim Hay, Maxim Frolov
JOURNAL OF CELL SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Xinyu Chen, Majd M. Ariss, Gopalakrishnan Ramakrishnan, Veronique Nogueira, Catherine Blaha, William Putzbach, Abul B. M. M. K. Islam, Maxim V. Frolov, Nissim Hay
Article
Multidisciplinary Sciences
David Brena, Joan Bertran, Montserrat Porta-de-la-Riva, Yolanda Guillen, Eric Cornes, Dmytro Kukhtar, Lluis Campos-Vicens, Lierni Fernandez, Irene Pecharroman, Albert Garcia-Lopez, Abul B. M. M. K. Islam, Laura Marruecos, Anna Bigas, Julian Ceron, Lluis Espinosa
SCIENTIFIC REPORTS
(2020)
Article
Biochemical Research Methods
Md Abdullah-Al-Kamran Khan, Rafeed Rahman Turjya, Abul Bashar Mir Md Khademul Islam
Summary: This study proposed a systematic in silico approach to design new variants of Adalimumab mAb with improved binding properties, resulting in one significantly improved variant out of 143 models. Six important residues were identified, and suitable mutations were applied to design significant variants, offering new insights for structure-based affinity improvements of monoclonal antibodies.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2021)
Article
Multidisciplinary Sciences
Alexandre F. Aissa, Abul B. M. M. K. Islam, Majd M. Ariss, Cammille C. Go, Alexandra E. Rader, Ryan D. Conrardy, Alexa M. Gajda, Carlota Rubio-Perez, Klara Valyi-Nagy, Mary Pasquinelli, Lawrence E. Feldman, Stefan J. Green, Nuria Lopez-Bigas, Maxim Frolov, Elizaveta Benevolenskaya
Summary: Tyrosine kinase inhibitors have clinical effectiveness in treating certain subsets of cancers with receptor tyrosine kinase somatic mutations, but limited duration of response and development of drug resistance are common issues. Single-cell RNA sequencing revealed multiple cancer cell subpopulations with epigenetic changes and variable therapeutic sensitivity. Overrepresented gene ontologies associated with drug tolerance include epithelial-to-mesenchymal transition, drug metabolism, and vesicle transport. Combination therapy targeting specific drug tolerant cell subpopulations shows promise in inhibiting drug resistance.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Nabiha Tasneem Khan, Maliha Afroj Zinnia, Abul Bashar Mir Md Khademul Islam
Summary: This study introduces an in silico designed mRNA vaccine to overcome the constraints of live-attenuated vaccines. Through prediction, simulation, and validation, the vaccine was found to have high immunogenicity and translational efficiency. Furthermore, specific T and B cell epitopes were identified that meet the criteria for an effective vaccine candidate, with high-affinity interactions between epitopes and HLA alleles.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Oncology
Dragana Kopanja, Vaibhav Chand, Eilidh O'Brien, Nishit K. Mukhopadhyay, Maria P. Zappia, Abul B. M. M. K. Islam, Maxim Frolov, Bradley J. Merrill, Pradip Raychaudhuri
Summary: This study provides new insights into the critical role of the interaction between FoxM1 and Rb in the plasticity and metastasis of breast cancer cells.
Article
Biochemistry & Molecular Biology
Sazzad Shahrear, Abul Bashar Mir Md Khademul Islam
Summary: This study evaluates a hypothetical tuberculosis mRNA vaccine using computational and immunoinformatics approaches. The findings suggest that the vaccine may elicit specific immune responses against Mycobacterium tuberculosis, and it serves as a reliable source for further wet-lab validation.
MOLECULAR DIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Sazzad Shahrear, Maliha Afroj Zinnia, Tasnim Ahmed, Abul Bashar Mir Md. Khademul Islam
Summary: Polyomaviruses may play a significant role in carcinogenesis by targeting multiple signaling pathways in the host and turning off tumor suppression genes through miRNA-induced mechanisms to promote cell transformation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Maria Paula Zappia, Yong-Jae Kwon, Anton Westacott, Isabel Liseth, Hyun Min Lee, Abul B. M. M. K. Islam, Jiyeon Kim, Maxim V. Frolov
Summary: The transcription factor E2F is responsible for controlling the expression of cell cycle genes by binding to their promoters. However, its significance in the regulation of metabolic genes is not well understood. In this study, the researchers introduced mutations in the E2F sites of five metabolic genes in fruit flies and found varying effects on E2F recruitment and gene expression, with the glycolytic gene Pgk being the most affected. Loss of E2F regulation on the Pgk gene led to decreased glycolytic flux, ATP content, and abnormal mitochondrial morphology. The study highlights the importance of E2F regulation on metabolic genes and its impact on overall development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Nilima Barman, Md Atiqul Haque, Mohammuddunobi Firoz, M. Abdullah Yusuf, Abul B. M. M. K. Islam
Summary: Polymorphism of TCF7L2 gene is associated with type 2 diabetes mellitus (T2DM) in the Bangladeshi population. The presence of rs12255372 (G > T) SNP in TCF7L2 increases the risk of developing T2DM. Individuals with the minor T allele have a higher likelihood of developing T2DM. Interaction with age, BMI, gender, and family history of diabetes mellitus further increases the risk.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Biochemical Research Methods
Supantha Dey, Sazzad Shahrear, Maliha Afroj Zinnia, Ahnaf Tajwar, Abul Bashar Mir Md Khademul Islam
Summary: This study provides functional annotation of hypothetical proteins (HPs) in Enterobacter cloacae B13 strain using bioinformatics tools. They identified several essential proteins and predicted some as virulent factors, which are associated with biofilm formation, quorum sensing, and signal transduction. Better understanding of these proteins' characteristics and functions can contribute to the knowledge and control of E. cloacae.
BIOINFORMATICS AND BIOLOGY INSIGHTS
(2022)
Article
Multidisciplinary Sciences
Abul Bashar Mir Md Khademul Islam, Md Abdullah-Al-Kamran Khan
SCIENTIFIC REPORTS
(2020)
Article
Genetics & Heredity
Abul Bashar Mir Md. Khademul Islam, Eusra Mohammad, Md. Abdullah-Al-Kamran Khan