Article
Cell Biology
Roberto Iorio, Giuseppe Celenza, Sabrina Petricca
Summary: Mitochondria are critical organelles for cellular energy balance and cell death. Mitophagy, the selective removal of dysfunctional mitochondria by autophagy, plays important roles in maintaining mitochondrial health. Both impaired and excessive mitophagy are involved in various ageing-associated diseases. Recent advances have explored the molecular mechanisms and signaling pathways that regulate mitophagy, including the early role and spatial specificity of the AMPK/ULK1 axis.
Article
Cell Biology
Fabienne C. Fiesel, Dominika Fricova, Caleb S. Hayes, Mathew A. Coban, Roman Hudec, Jenny M. Bredenberg, Benjamin J. Broadway, Briana N. Markham, Tingxiang Yan, Paige K. Boneski, Gabriella Fiorino, Jens O. Watzlawik, Xu Hou, Arthur M. McCarty, Laura J. Lewis-Tuffin, Jun Zhong, Benjamin J. Madden, Alban Ordureau, Heeseon An, Andreas Puschmann, Zbigniew K. Wszolek, Owen A. Ross, J. Wade Harper, Thomas R. Caulfield, Wolfdieter Springer
Summary: This study investigates the impact of single nucleotide variants in the activation loop of the PINK1 kinase on its enzymatic function. The G411A variant of PINK1 is found to significantly enhance Ub phosphorylation and promote PRKN activation and mitophagy, leading to increased neuronal viability under mitochondrial stress. The mechanism involves the stabilization of PINK1's kinase fold and a change in Ub conformation. These findings suggest that the G411A variant could have therapeutic potential for neuroprotection.
Article
Cell Biology
Jens O. Watzlawik, Fabienne C. Fiesel, Gabriella Fiorino, Bernardo A. Bustillos, Zahra Baninameh, Briana N. Markham, Xu Hou, Caleb S. Hayes, Jenny M. Bredenberg, Nicholas W. Kurchaba, Dominika Fricova, Joanna Siuda, Zbigniew K. Wszolek, Sachiko Noda, Shigeto Sato, Nobutaka Hattori, Asheeta A. Prasad, Deniz Kirik, Howard S. Fox, Kelly L. Stauch, Matthew S. Goldberg, Wolfdieter Springer
Summary: This study investigates the basal activation levels of the PINK1-PRKN signaling pathway in vivo using rodent samples, patient-derived cells, and isogenic neurons. The findings highlight the age-dependent, brain region-specific, and cell type-specific effects of PINK1-PRKN signaling, which have significant implications for improving diagnosis, prognosis, and patient stratification in Parkinson's disease.
Review
Chemistry, Multidisciplinary
Guy Mann, Pradeep Sadhu, Ashraf Brik
Summary: This article discusses recent advances in protein delivery methods, with a focus on those most compatible with synthetic proteins. Researchers have been working on developing protein delivery methods to study custom-made proteins in a biological context. These methods can help accurately determine the localization, degradation, folding, interactions, and involvement of proteins in membrane-less organelles formed by liquid-liquid phase separation inside cells.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Marta Vranas, Yang Lu, Shafqat Rasool, Nathalie Croteau, Jonathan D. Krett, Veronique Sauve, Kalle Gehring, Edward A. Fon, Thomas M. Durcan, Jean-Francois Trempe
Summary: Mutations in Parkin and PINK1 cause early-onset familial Parkinson's disease. The study found that His433 contributes to the catalysis of Parkin and its mutation impairs mitophagy. Mfn2 is a kinetically preferred substrate for Parkin, co-localizing with PINK1 and phospho-ubiquitin upon mitochondrial depolarization.
Article
Cell Biology
James L. Shen, Tina M. Fortier, Ruoxi Wang, Eric H. Baehrecke
Summary: Defects in autophagy can lead to issues in metabolism, development, and disease. The loss of Vps13D affects mitophagy, regulated by the core autophagy machinery. Pink1 and Vps13D play roles in Pink1-dependent mitophagy, with Park contributing to mitochondrial clearance through a pathway parallel to Vps13D.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ryota Hayashida, Reika Kikuchi, Kenichiro Imai, Waka Kojima, Tatsuya Yamada, Miho Iijima, Hiromi Sesaki, Keiji Tanaka, Noriyuki Matsuda, Koji Yamano
Summary: A new method was used to investigate the interaction between E2 and activated Parkin, leading to the identification of multiple E2s that interacted with Parkin during mitophagy. The study also revealed a consensus sequence for E2 interactions with activated Parkin. This method can be used to study the mechanisms involved in RBR-type E3s.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Rajesh Kumar, Andreas S. Reichert
Summary: Mitochondria are essential organelles in eukaryotic cells involved in various cellular functions, and dysfunction is linked to several diseases. Both yeast and mammals utilize similar mechanisms to regulate mitophagy, a process of degrading damaged mitochondria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Isshin Shiiba, Keisuke Takeda, Shun Nagashima, Naoki Ito, Takeshi Tokuyama, Shun-Ichi Yamashita, Tomotake Kanki, Toru Komatsu, Yasuteru Urano, Yuuta Fujikawa, Ryoko Inatome, Shigeru Yanagi
Summary: MITOL regulates Parkin-mediated cell death by promoting ubiquitination of Parkin at lysine 220 residue, leading to its proteasomal degradation and controlling mitophagy. Deletion of MITOL results in accumulation of active phosphorylated Parkin in the ER, causing FKBP38 degradation and increased cell death.
Article
Cell Biology
Takayuki Mito, Amy E. Vincent, Julie Faitg, Robert W. Taylor, Nahid A. Khan, Thomas G. McWilliams, Anu Suomalainen
Summary: The study finds that mitophagy plays a significant role in skeletal muscle mitochondrial dysfunction in aged mice and human patients. The progression of mitochondrial dysfunction interrupts mitophagy and disrupts lysosomal homeostasis. The mosaic halting of mitophagy may explain respiratory chain deficiency and accumulation of mtDNA variants in mitochondrial diseases and normal aging.
Article
Cell Biology
Jens O. Watzlawik, Xu Hou, Dominika Truban, Chloe Ramnarine, Sandeep K. Barodia, Tania F. Gendron, Michael G. Heckman, Michael DeTure, Joanna Siuda, Zbigniew K. Wszolek, Clemens R. Scherzer, Owen A. Ross, Guojun Bu, Dennis W. Dickson, Matthew S. Goldberg, Fabienne C. Fiesel, Wolfdieter Springer
Summary: Mitochondrial dysfunction is an early event in neurodegenerative disorders like Parkinson's and Alzheimer's disease. Researchers developed a sensitive ELISA tool to measure mitophagy levels and found that the PINK1-PRKN mitophagy pathway is actively involved in both mice and humans under various physiological and pathological conditions.
Article
Multidisciplinary Sciences
Katrin Stuber, Tobias Schneider, Jill Werner, Michael Kovermann, Andreas Marx, Martin Scheffner
Summary: The study reveals that NEDD8 can be phosphorylated at S65, affecting its structural dynamics similar to Ub. Phosphorylated NEDD8 has a distinct interactome compared to unmodified Ub and NEDD8, and it can more effectively regulate HSP70 protein activity.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Emma Rusilowicz-Jones, Francesco G. Barone, Fernanda Martins Lopes, Elezabeth Stephen, Heather Mortiboys, Sylvie Urbe, Michael J. Clague
Summary: Compound 39 inhibits USP30 and enhances mitophagy and pexophagy, providing a novel pharmacological intervention for enhancing peroxisome turnover. "This is the first pharmacological intervention with a synthetic molecule to enhance peroxisome turnover."
LIFE SCIENCE ALLIANCE
(2022)
Article
Genetics & Heredity
Irena J. J. Muffels, Imre F. Schene, Holger Rehmann, Maarten P. G. Massink, Maria M. van der Wal, Corinna Bauder, Martha Labeur, Natalia G. Armando, Maarten H. Lequin, Michiel L. Houben, Jaques C. Giltay, Saskia Haitjema, Albert Huisman, Fleur Vansenne, Judith Bluvstein, John Pappas, Lala V. Shailee, Yuri A. Zarate, Michal Mokry, Gijs W. van Haaften, Edward E. S. Nieuwenhuis, Damian Refojo, Femke van Wijk, Sabine A. Fuchs, Peter M. van Hasselt
Summary: Variants in NAE1 gene have been found to affect cellular pathways and disease pathophysiology. Decreased NAE1 abundance and overlapping clinical and cellular phenotypes support the pathogenicity of these variants. NAE1 is shown to play a role in protecting against cell death during cellular stress.
AMERICAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Multidisciplinary Sciences
Giovanni Quarato, Luigi Mari, Nicholas J. Barrows, Mao Yang, Sebastian Ruehl, Mark J. Chen, Cliff S. Guy, Jonathan Low, Taosheng Chen, Douglas R. Green
Summary: The degradation of defective mitochondria is regulated by the ubiquitin-proteasome system and lysosomal activities, which play essential roles in maintaining cellular homeostasis. Activation of the PINK1-Parkin axis following mitochondrial damage triggers a BAX- and BAK-independent cytochrome c release process, leading to apoptosis mediated by APAF1 and caspase 9. This process is initiated by UPS-dependent outer mitochondrial membrane degradation and can be reversed by proteasome inhibitors. Autophagy machinery recruitment to the outer mitochondrial membrane protects cells from apoptosis by mediating the lysosomal degradation of dysfunctional mitochondria. The study highlights the major role of the autophagy machinery in counteracting abnormal noncanonical apoptosis and identifies autophagy receptors as key regulators of this process.
Article
Biochemistry & Molecular Biology
Yusuke Sato, Kei Okatsu, Yasushi Saeki, Koji Yamano, Noriyuki Matsuda, Ai Kaiho, Atsushi Yamagata, Sakurako Goto-Ito, Minoru Ishikawa, Yuichi Hashimoto, Keiji Tanaka, Shuya Fukai
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Yukiko Yoshida, Sayaka Yasuda, Toshiharu Fujita, Maho Hamasaki, Arisa Murakami, Junko Kawawaki, Kazuhiro Iwai, Yasushi Saeki, Tamotsu Yoshimori, Noriyuki Matsuda, Keiji Tanaka
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biology
Koji Yamano, Chunxin Wang, Shireen A. Sarraf, Christian Muench, Reika Kikuchi, Nobuo N. Noda, Yohei Hizukuri, Masato T. Kanemaki, Wade Harper, Keiji Tanaka, Noriyuki Matsuda, Richard J. Youle
Article
Biochemistry & Molecular Biology
Shinya Tashiro, Jose M. M. Caaveiro, Makoto Nakakido, Aki Tanabe, Satoru Nagatoishi, Yasushi Tamura, Noriyuki Matsuda, Dali Liu, Quyen Q. Hoang, Kouhei Tsumoto
ACS CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Ayane Yamaguchi, Hayate Ishikawa, Mana Furuoka, Masashi Yokozeki, Noriyuki Matsuda, Susumu Tanimura, Kohsuke Takeda
JOURNAL OF BIOCHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Kei Okatsu, Yusuke Sato, Koji Yamano, Noriyuki Matsuda, Lumi Negishi, Akiko Takahashi, Atsushi Yamagata, Sakurako Goto-Ito, Masaki Mishima, Yutaka Ito, Toshihiko Oka, Keiji Tanaka, Shuya Fukai
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Fumika Koyano, Koji Yamano, Hidetaka Kosako, Keiji Tanaka, Noriyuki Matsuda
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Fumika Koyano, Koji Yamano, Hidetaka Kosako, Yoko Kimura, Mayumi Kimura, Yukio Fujiki, Keiji Tanaka, Noriyuki Matsuda
Review
Neurosciences
Noriyuki Matsuda, Koji Yamano
NEUROSCIENCE RESEARCH
(2020)
Article
Cell Biology
Koji Yamano, Reika Kikuchi, Waka Kojima, Ryota Hayashida, Fumika Koyano, Junko Kawawaki, Takuji Shoda, Yosuke Demizu, Mikihiko Naito, Keiji Tanaka, Noriyuki Matsuda
JOURNAL OF CELL BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Mashun Onishi, Koji Yamano, Miyuki Sato, Noriyuki Matsuda, Koji Okamoto
Summary: Mitophagy is a fundamental cellular mechanism for degrading mitochondria, regulated by specific receptors or ubiquitin molecules. Its dysfunction is associated with various pathological conditions, highlighting its biological significance in maintaining cellular homeostasis.
Article
Cell Biology
Waka Kojima, Koji Yamano, Hidetaka Kosako, Kenichiro Imai, Reika Kikuchi, Keiji Tanaka, Noriyuki Matsuda
Summary: Macroautophagy/autophagy is a complex intracellular degradation process with many regulatory factors. This study identified BCAS3 and C16orf70 as novel proteins associated with the autophagosome formation site and demonstrated their regulatory roles in autophagic activity. The BCAS3-C16orf70 complex affects the recruitment of core autophagy proteins to the phagophore assembly site.
Article
Biochemistry & Molecular Biology
Taiki Baba, Susumu Tanimura, Ayane Yamaguchi, Koichiro Horikawa, Masashi Yokozeki, Saki Hachiya, Shun-Ichiro Iemura, Tohru Natsume, Noriyuki Matsuda, Kohsuke Takeda
Summary: PGAM5 is a protein phosphatase located in the inner mitochondrial membrane, which is cleaved and released into the nucleus upon mitochondrial dysfunction. It interacts with nuclear protein SRm160, dephosphorylates it, and regulates phosphorylation of other SR proteins during mitophagy, potentially coordinating cellular responses to mitochondrial stress at the post-transcriptional and pretranslational levels.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yukiko Yoshida, Makoto Asahina, Arisa Murakami, Junko Kawawaki, Meari Yoshida, Reiko Fujinawa, Kazuhiro Iwai, Ryuichi Tozawa, Noriyuki Matsuda, Keiji Tanaka, Tadashi Suzuki
Summary: Mutation in the NGLY1 gene leads to a recessive disorder in humans. Findings in this study suggest that dysfunction of the proteasome caused by accumulation of N-glycoproteins, particularly NRF1 ubiquitinated by SCFFBS2, contributes to the pathogenesis resulting from NGLY1 deficiency.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Bruno Barros Queliconi, Waka Kojima, Mayumi Kimura, Kenichiro Imai, Chisato Udagawa, Chie Motono, Takatsugu Hirokawa, Shinya Tashiro, Jose M. M. Caaveiro, Kouhei Tsumoto, Koji Yamano, Keiji Tanaka, Noriyuki Matsuda
Summary: The study reveals that pathogenic mutations in the PARK7 gene encoding DJ-1 protein lead to its import into the mitochondrial matrix for degradation due to protein unfolding. Increasing structural stability of these mutants restores cytosolic localization, and a reduction in structural stability exposes a mitochondrial-targeting signal for DJ-1 import into the mitochondria.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)