Illuminating the catalytic core of ectoine synthase through structural and biochemical analysis

2-Oxoglutarate-Dependent Oxygenases

(2018) Md. Saiful Islam et al.   Annual Review of Biochemistry

Identification of osmoadaptive strategies in the halophile, heterotrophic ciliate Schmidingerothrix salinarum

(2018) Lea Weinisch et al.   PLOS BIOLOGY

With a pinch of extra salt—Did predatory protists steal genes from their food?

(2018) Laura Czech et al.   PLOS BIOLOGY

Role of the Extremolytes Ectoine and Hydroxyectoine as Stress Protectants and Nutrients: Genetics, Phylogenomics, Biochemistry, and Structural Analysis

(2018) Laura Czech et al.   Genes

Transcriptional regulation of ectoine catabolism in response to multiple metabolic and environmental cues

(2017) Annina Schulz et al.   ENVIRONMENTAL MICROBIOLOGY

Improved fermentative production of the compatible solute ectoine by Corynebacterium glutamicum from glucose and alternative carbon sources

(2017) Fernando Pérez-García et al.   JOURNAL OF BIOTECHNOLOGY

Osmotic Shock Induced Protein Destabilization in Living Cells and Its Reversal by Glycine Betaine

(2017) Samantha S. Stadmiller et al.   JOURNAL OF MOLECULAR BIOLOGY

Microorganisms maintain crowding homeostasis

(2017) Jonas van den Berg et al.   NATURE REVIEWS MICROBIOLOGY

Strangers in the archaeal world: osmostress-responsive biosynthesis of ectoine and hydroxyectoine by the marine thaumarchaeonNitrosopumilus maritimus

(2016) Nils Widderich et al.   ENVIRONMENTAL MICROBIOLOGY

Feeding on compatible solutes: A substrate-induced pathway for uptake and catabolism of ectoines and its genetic control by EnuR

(2016) Annina Schulz et al.   ENVIRONMENTAL MICROBIOLOGY

Osmoadaptative Strategy and Its Molecular Signature in Obligately Halophilic Heterotrophic Protists

(2016) Tommy Harding et al.   Genome Biology and Evolution

Pathway construction and metabolic engineering for fermentative production of ectoine in Escherichia coli

(2016) Yike Ning et al.   METABOLIC ENGINEERING

EctD-mediated biotransformation of the chemical chaperone ectoine into hydroxyectoine and its mechanosensitive channel-independent excretion

(2016) Laura Czech et al.   Microbial Cell Factories

KdgF, the missing link in the microbial metabolism of uronate sugars from pectin and alginate

(2016) Joanne K. Hobbs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Do microbial osmolytes or extracellular depolymerisation products accumulate as soil dries?

(2016) Charles R. Warren   SOIL BIOLOGY & BIOCHEMISTRY

Biochemistry and Crystal Structure of Ectoine Synthase: A Metal-Containing Member of the Cupin Superfamily

(2016) Nils Widderich et al.   PLoS One

Neutrons describe ectoine effects on water H-bonding and hydration around a soluble protein and a cell membrane

(2016) Giuseppe Zaccai et al.   Scientific Reports

Abiotic stress protection by ecologically abundant dimethylsulfoniopropionate and its natural and synthetic derivatives: insights fromBacillus subtilis

(2014) Sebastian Broy et al.   ENVIRONMENTAL MICROBIOLOGY

The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum

(2014) Katharina D. Moritz et al.   EXTREMOPHILES

Crystal Structure of the Ectoine Hydroxylase, a Snapshot of the Active Site

(2014) Astrid Höppner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biochemical Properties of Ectoine Hydroxylases from Extremophiles and Their Wider Taxonomic Distribution among Microorganisms

(2014) Nils Widderich et al.   PLoS One

Glass-forming property of hydroxyectoine is the cause of its superior function as a desiccation protectant

(2014) Christoph Tanne et al.   Frontiers in Microbiology

Membrane protein stability depends on the concentration of compatible solutes – a single molecule force spectroscopic study

(2013) Arpita Roychoudhury et al.   BIOLOGICAL CHEMISTRY

Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase

(2013) Ivan Hajnal et al.   FEBS Journal

Molecular Dynamics Simulations and Structure-Guided Mutagenesis Provide Insight into the Architecture of the Catalytic Core of the Ectoine Hydroxylase

(2013) Nils Widderich et al.   JOURNAL OF MOLECULAR BIOLOGY

Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine

(2013) Judith Becker et al.   Microbial Cell Factories

MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability

(2013) K. Katoh et al.   MOLECULAR BIOLOGY AND EVOLUTION

The genome portal of the Department of Energy Joint Genome Institute: 2014 updates

(2013) Henrik Nordberg et al.   NUCLEIC ACIDS RESEARCH

Protein Similarity Networks Reveal Relationships among Sequence, Structure, and Function within the Cupin Superfamily

(2013) Richard Uberto et al.   PLoS One

Response of osmolytes in soil to drying and rewetting

(2013) Charles R. Warren   SOIL BIOLOGY & BIOCHEMISTRY

Metabolites Associated with Adaptation of Microorganisms to an Acidophilic, Metal-Rich Environment Identified by Stable-Isotope-Enabled Metabolomics

(2013) A. C. Mosier et al.   mBio

Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens

(2012) Javier Rodríguez-Moya et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Complete Genome Sequence of Paenibacillus strain Y4.12MC10, a Novel Paenibacillus lautus strain Isolated from Obsidian Hot Spring in Yellowstone National Park

(2012) David A. Mead et al.   Standards in Genomic Sciences

REFMAC5 for the refinement of macromolecular crystal structures

(2011) Garib N. Murshudov et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC

(2011) Elisabeth M. H. J. Witt et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A Specialized Aspartokinase Enhances the Biosynthesis of the Osmoprotectants Ectoine and Hydroxyectoine in Pseudomonas stutzeriA1501

(2011) N. Stoveken et al.   JOURNAL OF BACTERIOLOGY

Ectoine and Hydroxyectoine as Protectants against Osmotic and Cold Stress: Uptake through the SigB-Controlled Betaine-Choline- Carnitine Transporter-Type Carrier EctT from Virgibacillus pantothenticus

(2011) A. U. Kuhlmann et al.   JOURNAL OF BACTERIOLOGY

Divergence and Convergence in Enzyme Evolution

(2011) Michael Y. Galperin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Interactive Tree Of Life v2: online annotation and display of phylogenetic trees made easy

(2011) I. Letunic et al.   NUCLEIC ACIDS RESEARCH

Integration, scaling, space-group assignment and post-refinement

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Bacterial Osmoregulation: A Paradigm for the Study of Cellular Homeostasis

(2010) Janet M. Wood   Annual Review of Microbiology

Natural and Engineered Hydroxyectoine Production Based on thePseudomonas stutzeri ectABCD-askGene Cluster

(2010) Britta Seip et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genome-Driven Investigation of Compatible Solute Biosynthesis Pathways of Pseudomonas syringae pv. syringae and Their Contribution to Water Stress Tolerance

(2010) M. Kurz et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolomic Characterization of the Salt Stress Response in Streptomyces coelicolor

(2010) S. Kol et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Ectoines in cell stress protection: Uses and biotechnological production

(2010) José M. Pastor et al.   BIOTECHNOLOGY ADVANCES

Cold adaptation in the marine bacterium, Sphingopyxis alaskensis, assessed using quantitative proteomics

(2010) Lily Ting et al.   ENVIRONMENTAL MICROBIOLOGY

A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581T

(2010) Karin Schwibbert et al.   ENVIRONMENTAL MICROBIOLOGY

Dali server: conservation mapping in 3D

(2010) Liisa Holm et al.   NUCLEIC ACIDS RESEARCH

Synthesis of 5-Hydroxyectoine from Ectoine: Crystal Structure of the Non-Heme Iron(II) and 2-Oxoglutarate-Dependent Dioxygenase EctD

(2010) Klaus Reuter et al.   PLoS One

The polyketide cyclase RemF fromStreptomyces resistomycificuscontains an unusual octahedral zinc binding site

(2009) Laura Silvennoinen et al.   FEBS LETTERS

The Crystal Structure of UehA in Complex with Ectoine—A Comparison with Other TRAP-T Binding Proteins

(2009) Justin Lecher et al.   JOURNAL OF MOLECULAR BIOLOGY

Cohesion Group Approach for Evolutionary Analysis of Aspartokinase, an Enzyme That Feeds a Branched Network of Many Biochemical Pathways

(2009) C.-C. Lo et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Structure-Based Phylogeny as a Diagnostic for Functional Characterization of Proteins with a Cupin Fold

(2009) Garima Agarwal et al.   PLoS One

Osmolyte-induced conformational changes in the Hsp90 molecular chaperone

(2009) Timothy O. Street et al.   PROTEIN SCIENCE

Synthesis and Uptake of the Compatible Solutes Ectoine and 5-Hydroxyectoine by Streptomyces coelicolor A3(2) in Response to Salt and Heat Stresses

(2008) J. Bursy et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Synthesis of the Compatible Solute Ectoine in Virgibacillus pantothenticus Is Triggered by High Salinity and Low Growth Temperature

(2008) A. U. Kuhlmann et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

1.55 Å Structure of the Ectoine Binding Protein TeaA of the Osmoregulated TRAP-Transporter TeaABC fromHalomonas elongata†‡

(2008) Sonja I. Kuhlmann et al.   BIOCHEMISTRY

The multifunctional role of ectoine as a natural cell protectant

(2008) Ruediger Graf et al.   CLINICS IN DERMATOLOGY