Article
Multidisciplinary Sciences
Alejandro Lopez Gonzalez, Lea Luciana, Clementine Le Neve, Julie Valantin, Laura Francols, Nicolas Gadot, Christophe Vanbelle, Laurianne Davignon, Laura Broutier
Summary: This paper presents detailed protocols for staining and subcellular resolution imaging of fixed in vitro 3D cell culture models, applicable to a wide variety of organoids and spheroids, that can be completed within 4-5 days from structure harvesting to image analysis.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Chemistry, Analytical
Ryan E. Leighton, Ariel M. Alperstein, Renee R. Frontiera
Summary: Super-resolution techniques have been developed to address nanoscale heterogeneities in biological and material samples that cannot be resolved with conventional microscopy techniques. In recent years, progress has been made in developing label-free super-resolution techniques, which draw inspiration from successful fluorescence-based methods. These techniques aim to achieve resolutions comparable to fluorescence-based methods and are currently facing challenges.
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Mohammad Ebrahim Asadi Zarch, Alireza Afshar, Farhad Rahmanifar, Mohammad Reza Jafarzadeh Shirazi, Mandana Baghban, Mohammad Dadpasand, Farzad Mohammad Rezazadeh, Arezoo Khoradmehr, Hossein Baharvand, Amin Tamadon
Summary: This study investigated the development of ovarian nervous network in mice using 2D and 3D evaluations. It found an increase in the number and area of ganglia during follicular growth and corpora lutea development. However, there were differences in some ratios between 2D and 3D results, highlighting the importance of 3D analysis for evaluating ovarian function.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Matteo Pedrazzoli, Manuela Medelin, Federica Marchiotto, Barbara Cisterna, Manuela Malatesta, Mario Buffelli
Summary: Utilizing multiple analysis techniques on a single sample is desirable in research to reduce animal use and maximize information. This study presents a simplified brain tissue staining procedure allowing for multiple analyses in the same sample without requiring special equipment or skills.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biology
Siqi Chen, Guangcai Liu, Anan Li, Zhixiang Liu, Ben Long, Xiaoquan Yang, Hui Gong, Xiangning Li
Summary: Dissecting anatomical information at single-cell level is crucial for understanding biological tissues. The array-fMOST method allows for the identification of three-dimensional information at single-cell resolution from multiple samples. This method can image and analyze multiple mouse brains simultaneously, providing valuable insights into neuronal distribution and changes in microenvironments.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Physical
Barun Kumar Maity, Duncan Nall, Yongjae Lee, Paul R. Selvin
Summary: Point Accumulation for Imaging in Nanoscale Topography (PAINT) is a single-molecule technique that achieves super-resolution microscopy with a resolution of approximately 5-25 nanometers by using exchangeable DNA oligos or peptide-pairs to create blinking phenomenon. By transfecting the protein-of-interest with a docker-coil, similar localization can be achieved at approximately 10 nm compared to using external dockers. This method allows for imaging surface proteins in live cells and provides enhanced resolution for organelles in fixed cells.
Article
Radiology, Nuclear Medicine & Medical Imaging
Miguel O. Castro, Vasco V. Mascarenhas, P. Diana Afonso, Paulo Rego, Florian Schmaranzer, Reto Sutter, Ara Kassarjian, Luca Sconfienza, Michael Dienst, Olufemi R. Ayeni, Paul E. Beaule, Pedro Dantas, Radhesh Lalam, Marc-Andre Weber, Filip M. Vanhoenacker, Tobias Johannes Dietrich, Lennart Jans, Philip Robinson, Apostolos H. Karantanas, Iwona Sudol-Szopinska, Suzanne Anderson, Iris Noebauer-Huhmann, Oliver Marin-Pena, Diego Collado, Marc Tey-Pons, Ehrenfried Schmaranzer, Mario Padron, Josef Kramer, Patrick O. Zingg, Michel De Maeseneer, Eva Llopis
Summary: The study established the first international consensus on FAI imaging using the Delphi method with 30 panel members. A total of 47 statements were generated, with group consensus achieved for 45 of them. The recommendations may assist researchers and clinicians in developing evidence-based imaging protocols and classifications for FAI and hip-related pain management.
EUROPEAN RADIOLOGY
(2021)
Article
Engineering, Multidisciplinary
Fei Lu, Bo Li, Shuying Wang, Zhaohui Hu, Mao Ye, Jixi Lu, Bangcheng Han
Summary: This paper proposes a two-dimensional scanning imaging configuration for rapid biaxial magnetic field measurement with femto-Tesla sensitivity. The configuration utilizes Bragg diffraction and a non-modulated time-sharing technique to achieve 2D magnetic field detection with millisecond temporal resolution and low power consumption.
Article
Optics
Yongjin Na, Hyunsoo Kwak, Changmin Ahn, Seung Eon Lee, Woojin Lee, Chu-Shik Kang, Jungchul Lee, Junho Suh, Hongki Yoo, Jungwon Kim
Summary: High-speed and high-resolution imaging of surface profiles is essential for studying various structures and mechanical dynamics in micro- and nano-scale devices. However, capturing real-time and complex mechanical dynamics has been challenging. In this study, we present a line-scan time-of-flight (TOF) camera that can measure the TOF changes of over 1000 spatial coordinates simultaneously, with high pixel-rate and sub-nanometer axial resolution. This camera enables fast and precise imaging of complex structures and dynamics in three-dimensional devices and mechanical resonators.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Oncology
Simon F. Merz, Philipp Jansen, Ricarda Ulankiewicz, Lea Bornemann, Tobias Schimming, Klaus Griewank, Zuelal Cibir, Andreas Kraus, Ingo Stoffels, Timo Aspelmeier, Sven Brandau, Dirk Schadendorf, Eva Hadaschik, Gernot Ebel, Matthias Gunzer, Joachim Klode
Summary: This study utilized LSFM technology to comprehensively analyze SLNs of melanoma patients, revealing metastases undetectable by routine histopathological assessment and improving the accuracy of detection and localization of metastatic cancer, potentially providing additional therapeutic options for patients.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Optics
Zhenfei Jiao, Zhou Zhou, Zhongyun Chen, Jinze Xie, Yu Mu, Jiulin DU, Ling Fu
Summary: To understand the functional neural circuits, it is crucial to acquire the firing sequence of neurons at different depths with cellular resolution. The Simple Multi-Plane Imaging Light-Sheet Fluorescence Microscopy (SIMPLE-LSFM) combines multi-plane illumination and spherical-aberration-assisted point spread function to simultaneously image neurons at different depths. This method was successfully demonstrated in capturing the firing sequences of neurons at six depths in larval zebrafish, providing fast and high-resolution mapping of anatomical structure and dynamics.
Article
Biochemical Research Methods
Assel Akhmetova, Igor Yaminsky
Summary: Scanning probe microscopy provides 3D visualization of viruses in different conditions and measurement of their properties. The focus is on high-resolution visualization of plant viruses, some of which are not harmful to humans. Despite visible progress, many unresolved issues remain in this field.
Review
Plant Sciences
Yoko Ito, Tomohiro Uemura
Summary: Compared to the static image of plants, the world inside each cell is dynamic. Visualizing the working components inside cells using super-resolution microscopy is crucial for understanding cellular events, particularly in the study of plant membranes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Moeko Sato, Hiroko Akashi, Yuki Sakamoto, Sachihiro Matsunaga, Hiroyuki Tsuji
Summary: This study established a protocol for tissue clearing and 3D imaging in rice, allowing for cellular-resolution 3D imaging of rice shoot apical meristems, florets, and root apical meristems. Using fluorescent reporters, the 3D distribution of auxin signaling events in rice root tips was revealed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jason C. Sang, Eric Hidari, Georg Meisl, Rohan T. Ranasinghe, Maria Grazia Spillantini, David Klenerman
Summary: Researchers found that cells respond to disruption of protein homeostasis by alpha-synuclein seeds primarily by secreting nanoscopic aggregates. This mechanism may represent an important protective response by cells to protein aggregation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Allergy
Bastiaan Maes, Ursula Smole, Matthias Vanderkerken, Kim Deswarte, Justine Van Moorleghem, Karl Vergote, Manon Vanheerswynghels, Caroline De Wolf, Sofie De Prijck, Nincy Debeuf, Benjamin Pavie, Wendy Toussaint, Sophie Janssens, Savvas Savvides, Bart N. Lambrecht, Hamida Hammad
Summary: This study found that loss of Taok3 can alleviate house dust mite-driven allergic asthma due to defects in both adaptive dendritic cell-mediated T(H)2 activation and innate ILC2 function.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Correction
Biochemical Research Methods
Ulrike Boehm, Glyn Nelson, Claire M. Brown, Steve Bagley, Peter Bajcsy, Johanna Bischof, Aurelien Dauphin, Ian M. Dobbie, John E. Eriksson, Orestis Faklaris, Julia Fernandez-Rodriguez, Alexia Ferrand, Laurent Gelman, Ali Gheisari, Hella Hartmann, Christian Kukat, Alex Laude, Miso Mitkovski, Sebastian Munck, Alison J. North, Tobias M. Rasse, Ute Resch-Genger, Lucas C. Schuetz, Arne Seitz, Caterina Strambio-De-Castillia, Jason R. Swedlow, Roland Nitschke
Article
Plant Sciences
Reinout Laureyns, Jessica Joossens, Denia Herwegh, Julie Pevernagie, Benjamin Pavie, Kirin Demuynck, Kevin Debray, Griet Coussens, Laurens Pauwels, Tom Van Hautegem, Michiel Bontinck, Josh Strable, Hilde Nelissen
Summary: The study optimized in situ sequencing (ISS) to simultaneously locate the transcripts of 90 genes in maize shoot apex, providing highly comparable expression profiles and allowing discrimination between tissue domains. The use of ISS in the shoot apex revealed spatiotemporal transcriptional events. The expression patterns of PLASTOCHRON1 (PLA1) demonstrated the effectiveness of the technology in identifying specific gene expression at cell boundaries. Spatial transcriptomics has significant applications in plant developmental biology research.
Editorial Material
Biochemistry & Molecular Biology
Saskia Lippens, Dominique Audenaert, Alexander Botzki, Stefaan Derveaux, Bart Ghesquiere, Geert Goeminne, Reza Hassanzadeh, Jurgen Haustraete, Francis Impens, Jochen Lamote, Sebastian Munck, Niels Vandamme, Gert Van Isterdael, Marijke Lein, Geert Van Minnebruggen
Review
Microscopy
Sebastian Munck, Christopher Cawthorne, Abril Escamilla-Ayala, Axelle Kerstens, Sergio Gabarre, Katrina Wesencraft, Eliana Battistella, Rebecca Craig, Emmanuel G. Reynaud, Jim Swoger, Gail McConnell
Summary: Optical mesoscale imaging is a rapidly developing field that bridges the gap between cell and organism resolution, allowing visualization of larger samples. However, challenges arise with larger specimens, including distortions and shadowing. This manuscript discusses the latest developments in mesoscale imaging, highlights limitations, and explores methods to turn it into a more quantitative technique, involving digital and physical phantoms as well as artificial intelligence.
JOURNAL OF MICROSCOPY
(2022)
Article
Immunology
Lidia Yshii, Emanuela Pasciuto, Pascal Bielefeld, Loriana Mascali, Pierre Lemaitre, Marika Marino, James Dooley, Lubna Kouser, Stijn Verschoren, Vasiliki Lagou, Hannelore Kemps, Pascal Gervois, Antina de Boer, Oliver T. Burton, Jerome Wahis, Jens Verhaert, Samar Tareen, Carlos P. Roca, Kailash Singh, Carly E. Whyte, Axelle Kerstens, Zsuzsanna Callaerts-Vegh, Suresh Poovathingal, Teresa Prezzemolo, Keimpe Wierda, Amy Dashwood, Junhua Xie, Elien Van Wonterghem, Eline Creemers, Meryem Aloulou, Willy Gsell, Oihane Abiega, Sebastian Munck, Roosmarijn E. Vandenbroucke, Annelies Bronckaers, Robin Lemmens, Bart De Strooper, Ludo Van Den Bosch, Uwe Himmelreich, Carlos P. Fitzsimons, Matthew G. Holt, Adrian Liston
Summary: The ability of immune-modulating biologics to prevent and reverse pathology has transformed recent clinical practice. In this study, researchers identified brain interleukin 2 (IL-2) levels as a limiting factor for brain-resident T-reg cells and developed a gene-delivery approach to astrocytes to increase IL-2 production. Mice with brain-specific IL-2 delivery were protected against neuroinflammation, demonstrating the effectiveness of this approach.
Article
Cell Biology
Dick W. Zijlmans, Irene Talon, Sigrid Verhelst, Adam Bendall, Karlien Van Nerum, Alok Javali, Andrew A. Malcolm, Sam S. F. A. van Knippenberg, Laura Biggins, San Kit To, Adrian Janiszewski, Danielle Admiraal, Ruth Knops, Nikky Corthout, Bradley P. Balaton, Grigorios Georgolopoulos, Amitesh Panda, Natarajan Bhanu, Amanda J. Collier, Charlene Fabian, Ryan N. Allsop, Joel Chappell, Thi Xuan Ai Pham, Michael Oberhuemer, Cankat Ertekin, Lotte Vanheer, Paraskevi Athanasouli, Frederic Lluis, Dieter Deforce, Joop H. Jansen, Benjamin A. Garcia, Michiel Vermeulen, Nicolas Rivron, Maarten Dhaenens, Hendrik Marks, Peter J. Rugg-Gunn, Vincent Pasque
Summary: This study reveals that human naive pluripotent stem cells possess chromatin mechanisms, specifically the H3K27me3 deposited by polycomb repressive complex 2 (PRC2), that restrict differentiation into trophoblasts. PRC2 activity acts as a chromatin barrier opposing trophoblast lineage induction.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Panagiotis Karras, Ignacio Bordeu, Joanna Pozniak, Ada Nowosad, Cecilia Pazzi, Nina Van Raemdonck, Ewout Landeloos, Yannick Van Herck, Dennis Pedri, Greet Bervoets, Samira Makhzami, Jia Hui Khoo, Benjamin Pavie, Jochen Lamote, Oskar Marin-Bejar, Michael Dewaele, Han Liang, Xingju Zhang, Yichao Hua, Jasper Wouters, Robin Browaeys, Gabriele Bergers, Yvan Saeys, Francesca Bosisio, Joost van den Oord, Diether Lambrechts, Anil K. Rustgi, Oliver Bechter, Cedric Blanpain, Benjamin D. Simons, Florian Rambow, Jean-Christophe Marine
Summary: By combining mouse genetics, single-cell and spatial transcriptomics, lineage tracing and quantitative modelling, the researchers provide evidence of a hierarchical model of tumour growth in melanoma and reveal the spatial and temporal diversity and trajectories of melanoma cell states. They show that tumorigenic competence is associated with a spatially localized perivascular niche and that only a fraction of cells are fated to fuel primary tumour growth, while another fraction constitutes a pool of metastatic initiating cells that switch cell identity during dissemination to secondary organs. These findings highlight the importance of targeting microenvironmental cues to interfere with the cancer cell reprogramming activity.
Article
Biology
Helene De Bruyn, Nikky Corthout, Sebastian Munck, Wouter Everaerts, Thomas Voets
Summary: Understanding the lower urinary tract (LUT) is crucial for developing novel therapies for LUT disorders. We developed a videocystometry technique to accurately monitor the LUT in rodents, but it had limitations in awake and freely moving animals. To overcome this, we used machine learning to automatically analyze bladder filling and voiding cycles in behaving mice. Our findings revealed the effects of anesthesia on urethral relaxation and voiding duration, as well as the decreased bladder capacity after surgical implantation of a catheter. This approach provides a paradigm for noninvasive, in vivo monitoring of hollow organs in behaving animals and highlights the limitations of current standard techniques for studying the LUT in mice.
Article
Neurosciences
Julie Jacquemyn, Sabine Kuenen, Jef Swerts, Benjamin Pavie, Vinoy Vijayan, Ayse Kilic, Dries Chabot, Yu-Chun Wang, Nils Schoovaerts, Nikky Corthout, Patrik Verstreken
Summary: Recent research shows a link between dysfunctional lipid metabolism and the development of Parkinson's disease. Through a new Drosophila model, researchers found that a pathogenic mutation caused synaptic dysfunction, neurological defects, neurodegeneration, and specific lipid metabolism alterations. Overexpression of another protein mutated in Parkinson's disease rescued the lipid alterations, neuronal function defects, and neurodegeneration, suggesting a relationship between these two proteins and phosphoinositide metabolism in maintaining neuronal integrity and survival.
NPJ PARKINSONS DISEASE
(2023)
Article
Gastroenterology & Hepatology
Dieudonne Buh Kum, Hannah Vanrusselt, Abel Acosta Sanchez, Valerio Taverniti, Eloi R. Verrier, Thomas F. Baumert, Cheng Liu, Jerome Deval, Nikky Corthout, Sebastian Munck, Leonid Beigelman, Lawrence M. Blatt, Julian A. Symons, Pierre Raboisson, Andreas Jekle, Sandrine Vendeville, Yannick Debing
Summary: This study uncovers a novel mechanism of action for CAM-As in the treatment of chronic hepatitis B, where HBc aggregation induces cell death, leading to hepatocyte proliferation and loss of covalently closed circular DNA or its equivalent, possibly assisted by an induced innate immune response.
Article
Cell Biology
Maria Livia Sassano, Alexander R. R. van Vliet, Ellen Vervoort, Sofie Van Eygen, Chris van den Haute, Benjamin Pavie, Joris Roels, Johannes V. V. Swinnen, Marco Spinazzi, Leen Moens, Kristina Casteels, Isabelle Meyts, Paolo Pinton, Saverio Marchi, Leila Rochin, Francesca Giordano, Blanca Felipe-Abrio, Patrizia Agostinis
Summary: ER-mitochondria appositions play a critical role in the transfer of ions and phospholipids between these organelles and impact mitochondrial bioenergetics. However, the molecular mechanisms involved in this process are not well understood. This study reveals that PERK acts as an adaptor to recruit the lipid transfer protein E-Syt1 at ER-mitochondria contact sites, enabling lipid transport and maintaining mitochondrial homeostasis. These findings provide insights into the molecular components and functions of the lipid trafficking machinery at ER-mitochondria contact sites.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Virology
Hannah Vanrusselt, Dieudonne Buh Kum, Valerio Taverniti, Cheng Liu, Abel Acosta Sanchez, Nikky Corthout, Sebastian Munck, Thomas F. Baumert, Leonid Beigelman, Lawrence M. Blatt, Julian A. Symons, Jerome Deval, Pierre Raboisson, Eloi R. Verrier, Andreas Jekle, Sandrine Vendeville, Yannick Debing
Summary: This study reports on the first non-HAP CAM-As (capsid assembly modulators) ALG-005398 and ALG-005863, providing a detailed in vitro intracellular characterization. These non-HAP CAM-As showed potent inhibition of HBV DNA production and blocked the establishment of cccDNA.
JOURNAL OF VIROLOGY
(2023)
Meeting Abstract
Cell Biology
M. Bretou, R. Sannerud, A. Escamilla-Ayala, E. Eskelinen, Z. P. Van Acker, B. Pavie, W. Annaert
MOLECULAR BIOLOGY OF THE CELL
(2023)
