Article
Oncology
Mingchen Shi, Yu Wang, Dong Lin, Yuzhuo Wang
Summary: Patient-derived xenografts (PDXs) have gained significant attention in cancer research as relevant models due to their retention of histopathological and molecular heterogeneity from donor tumors. Neuroendocrine prostate cancer (NEPC) PDX models are increasingly being generated and have shown utility in various research applications. This review provides a comprehensive overview of NEPC PDX collections, highlighting their potential for identifying therapeutic targets and evaluating drug responses.
Review
Oncology
Binhua Pan, Xuyong Wei, Xiao Xu
Summary: Animal models are essential for evaluating human cancers and investigating anticancer drugs and prevention. In hepatopancreatobiliary cancer research, mouse models are widely used. While current CDX models have limitations, the emerging PDX model shows promise for precision cancer medicine in hepatopancreatobiliary cancer research.
CANCER CELL INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Anna Rogojina, Laura J. Klesse, Erin Butler, Jiwoong Kim, He Zhang, Xue Xiao, Lei Guo, Qinbo Zhou, Taylor Hartshorne, Dawn Garcia, Korri Weldon, Trevor Holland, Abhik Bandyopadhyay, Luz Perez Prado, Shidan Wang, Donghan M. Yang, Anne-Marie Langevan, Yi Zou, Allison C. Grimes, Chatchawin Assanasen, Vinod Gidvani-Diaz, Siyuan Zheng, Zhao Lai, Yidong Chen, Yang Xie, Gail E. Tomlinson, Stephen X. Skapek, Raushan T. Kurmasheva, Peter J. Houghton, Lin Xu
Summary: Patient-derived xenografts (PDX) are valuable tools for studying biology and developing new therapies. In this study, researchers successfully developed PDX models from Hispanic childhood leukemia patients and found that these models accurately reflected the genetic abnormalities and expression patterns found in the primary tumors.
Review
Biochemistry & Molecular Biology
Vittoria Rago, Anna Perri, Silvia Di Agostino
Summary: One of the major goals in cancer research is to develop new anticancer therapies. Preclinical in vitro models, such as patient-derived xenograft (PDX) models and cultured organoids derived from patients (PDO), have become indispensable tools for understanding cancer progression, drug resistance, and metastasis. This article reviews studies from the past 10 years on the use of PDX models and PDOs in studying prostate cancer, which is characterized by genomic instability and resistance to conventional therapies. The limitations and new challenges in using these models for drug testing and developing molecular therapies are also discussed.
Review
Medicine, Research & Experimental
Shahrokh Abdolahi, Zeinab Ghazvinian, Samad Muhammadnejad, Mahshid Saleh, Hamid Asadzadeh Aghdaei, Kaveh Baghaei
Summary: The establishment and application of patient-derived xenograft models have played a significant role in cancer treatment. These models accurately represent the biological characteristics of different cancers and have great value in the development of anti-cancer drugs and personalized medicine.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Hua Sun, Song Cao, R. Jay Mashl, Chia-Kuei Mo, Simone Zaccaria, Michael C. Wendl, Sherri R. Davies, Matthew H. Bailey, Tina M. Primeau, Jeremy Hoog, Jacqueline L. Mudd, Dennis A. Dean, Rajesh Patidar, Li Chen, Matthew A. Wyczalkowski, Reyka G. Jayasinghe, Fernanda Martins Rodrigues, Nadezhda Terekhanova, Yize Li, Kian-Huat Lim, Andrea Wang-Gillam, Brian A. Van Tine, Cynthia X. Ma, Rebecca Aft, Katherine C. Fuh, Julie K. Schwarz, Jose P. Zevallos, Sidharth Puram, John F. Dipersio, Nci PDXNet Consortium, Brandi Davis-Dusenbery, Matthew J. Ellis, Michael T. Lewis, Michael A. Davies, Meenhard Herlyn, Bingliang Fang, Jack A. Roth, Alana L. Welm, Bryan E. Welm, Funda Meric-Bernstam, Feng Chen, Ryan C. Fields, Shunqiang Li, Ramaswamy Govindan, James H. Doroshow, Jeffrey A. Moscow, Yvonne A. Evrard, Jeffrey H. Chuang, Benjamin J. Raphael, Li Ding
Summary: This study presents genomic landscapes of 536 patient-derived xenograft (PDX) models across 25 cancer types, providing insights into their molecular features and suitability for clinical trials. PDXs typically have higher purity than human tumors and are useful for investigating dynamic driver events and molecular properties, making them valuable tools in cancer research and drug development.
NATURE COMMUNICATIONS
(2021)
Article
Endocrinology & Metabolism
Lukas Clemens Bockelmann, Vera Freytag, Ann-Kristin Ahlers, Hanna Maar, Tobias Gosau, Anke Baranowsky, Rudiger Schmitz, Klaus Pantel, Udo Schumacher, Marie-Therese Haider, Tobias Lange
Summary: Bone metastases in castration-resistant prostate cancer (PCa) are influenced by the interaction between the bone microenvironment and tumor cells. Androgen-deprivation therapy and zoledronic acid may affect these interactions and the growth of disseminated tumor cells in the bone marrow. In this study, a xenograft tumor model was used to simulate the clinical situation of androgen-deprivation therapy combined with tumor resection. The effects of interventions and ZA treatment on tumor cell dissemination and bone remodeling were analyzed. Zoledronic acid prevented bone loss but had no effect on bone metastasis burden, while it reduced lung metastasis.
Review
Chemistry, Medicinal
Xiang Li, Minyong Li
Summary: Developing effective drug screening methods is crucial for antitumor drug research. Patient-derived xenograft (PDX) tumor animal models and zebrafish as a screening model organism enable accurate and rapid discovery of lead compounds in the preclinical stage, while elucidating tumor pathogenesis.
MEDICINAL RESEARCH REVIEWS
(2023)
Article
Oncology
Ramesh Butti, Prachi Kapse, Garima Bhadauriya, Suhail Ahmad, Rohan Chaubal, Pallavi Parab, Rasika Kadam, Soumya S. Mahapatra, Tanuja Shet, Amit Dutt, Sudeep Gupta, Gopal C. Kundu
Summary: By establishing and characterizing patient-derived orthotopic xenografts (PDOXs), researchers successfully identified hormone-resistant breast cancer and demonstrated the reliability and usefulness of these models for biomarker discovery and preclinical drug screening.
Article
Biochemistry & Molecular Biology
Xiaoqiang Wang, Desiree Ha, Hitomi Mori, Shiuan Chen
Summary: The study demonstrates that white button mushroom may inhibit prostate cancer by suppressing androgen receptor signaling pathways and interfering with cell-cycle control pathways. Additionally, a conjugated linoleic acid isomer was identified as a strong androgen receptor antagonist, potentially contributing to the prevention and treatment of prostate cancer.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Oncology
Alex Q. Lee, Masami Ijiri, Ryan Rodriguez, Regina Gandour-Edwards, Joyce Lee, Clifford G. Tepper, Yueju Li, Laurel Beckett, Kit Lam, Neal Goodwin, Noriko Satake
Summary: A metastatic PE-derived RMC PDX model was successfully established, maintaining histologic features of the patient cancer. Molecular integrity was well maintained between renal tumor and PE PDXs, as well as between two generations of renal tumor PDXs. Sunitinib demonstrated therapeutic efficacy for RMC using the PE PDX model.
FRONTIERS IN ONCOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Hsiao-Li Chuang, Yi-Chih Chang, Yi-Ting Huang, Jiunn-Wang Liao, Pei-Ling Kao, Yi-Fei Chen, Bin-Yin Lin, Yi-Lo Lin, Ter-Hsin Chen, Yu-Chih Wang
Summary: The study successfully established a PDX model for feline mammary tumors, preserving important features of the original tumor, including distant metastasis. This provides a platform for translational research on FMT.
Review
Biochemistry & Molecular Biology
Tomohito Tanaka, Ruri Nishie, Shoko Ueda, Shunsuke Miyamoto, Sousuke Hashida, Hiromi Konishi, Shinichi Terada, Yuhei Kogata, Hiroshi Sasaki, Satoshi Tsunetoh, Kohei Taniguchi, Kazumasa Komura, Masahide Ohmichi
Summary: A systematic review of publications on PDX models of cervical cancer reveals that severe immunodeficient mice and subrenal capsule implantation lead to higher engraftment rates; fragments sized 1-3 mm(3) are suitable for CC-PDX; no clear association was found between tumor and donor patient characteristics and engraftment rates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Brendon M. Patierno, Wen-Chi Foo, Tyler Allen, Jason A. Somarelli, Kathryn E. Ware, Santosh Gupta, Sandra Wise, John P. Wise, Xiaodi Qin, Dadong Zhang, Lingfan Xu, Yanjing Li, Xufeng Chen, Brant A. Inman, Shannon J. McCall, Jiaoti Huang, Rick A. Kittles, Kouros Owzar, Simon Gregory, Andrew J. Armstrong, Daniel J. George, Steven R. Patierno, David S. Hsu, Jennifer A. Freedman
Summary: This study established and characterized a PCPDX model from a prostate cancer tissue of a patient of African American ancestry, and comprehensively studied its pathology, genetic mutations, drug sensitivity, etc. The results showed that this PCPDX model is resistant to docetaxel, sensitive to cisplatin, and capable of experimental metastasis to the lungs in mice.
PROSTATE CANCER AND PROSTATIC DISEASES
(2022)
Review
Biochemistry & Molecular Biology
Yihan Liu, Wantao Wu, Changjing Cai, Hao Zhang, Hong Shen, Ying Han
Summary: Patient-derived xenograft (PDX) models, which involve implanting tumor tissues from patients into mice, have proven to be superior in replicating the characteristics of cancer. They retain the genomic features of patients and have enabled a comprehensive understanding of the molecular landscape of PDX models. PDX models are invaluable in cancer treatment studies, such as preclinical trials, drug validation, patient screening, and exploring drug resistance mechanisms.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Su Jung Oh-Hohenhorst, Derya Tilki, Ann-Kristin Ahlers, Anna Suling, Oliver Hahn, Pierre Tennstedt, Christiane Matuszcak, Hanna Maar, Vera Labitzky, Sandra Hanika, Sarah Starzonek, Simon Baumgart, Steven A. Johnsen, Martina Kluth, Hueseyin Sirma, Ronald Simon, Guido Sauter, Hartwig Huland, Udo Schumacher, Tobias Lange
Summary: The deletion of the tumor suppressor gene CHD1 has been found to increase the risk of postoperative metastasis and promote spontaneous metastasis formation in prostate cancer patients after R0 resection. Additionally, CHD1 loss affects intratumoral recurrence and cancer-specific survival time in these patients.
CANCER GENE THERAPY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Jane In Den Birken, Laura Rathjens, Tina Rohlfing, Gunhild von Amsberg, Konstantina Stathopoulou, Sergey Dyshlovoy, Friederike Cuello
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Hermann Ehrlich, Magdalena Luczak, Rustam Ziganshin, Ivan Miksik, Marcin Wysokowski, Paul Simon, Irena Baranowska-Bosiacka, Patrycja Kupnicka, Alexander Ereskovsky, Roberta Galli, Sergey Dyshlovoy, Jonas Fischer, Konstantin R. Tabachnick, Iaroslav Petrenko, Teofil Jesionowski, Anna Lubkowska, Marek Figlerowicz, Viatcheslav N. Ivanenko, Adam P. Summers
Summary: Actin is an ancient intracellular protein that plays a crucial role in cell functions and also affects biosilica deposition in metazoans.
Article
Biochemistry & Molecular Biology
Gunhild von Amsberg, Winfried Alsdorf, Panagiotis Karagiannis, Anja Coym, Moritz Kaune, Stefan Werner, Markus Graefen, Carsten Bokemeyer, Lina Merkens, Sergey A. Dyshlovoy
Summary: Immunotherapeutic approaches have become essential in treating solid tumors, but face challenges in prostate cancer due to an immunosuppressive tumor microenvironment. Despite the limitations, a better understanding of tumor-immune system interactions offers hope for optimizing immunotherapy strategies in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Maxim E. Zhidkov, Moritz Kaune, Alexey Kantemirov, Polina A. Smirnova, Pavel Spirin, Maria A. Sidorova, Sergey A. Stadnik, Elena Y. Shyrokova, Dmitry N. Kaluzhny, Oleg A. Tryapkin, Tobias Busenbender, Jessica Hauschild, Tina Rohlfing, Vladimir S. Prassolov, Carsten Bokemeyer, Markus Graefen, Gunhild von Amsberg, Sergey A. Dyshlovoy
Summary: We synthesized a library of fascaplysin derivatives and investigated their selectivity, effects on cells, and DNA intercalation. The di- and trisubstituted halogen derivatives showed pronounced selectivity for cancer cells, while modification of cycle C resulted in stronger activity against therapy-resistant cells.
Article
Oncology
Retno Ningsi, Maha Elazezy, Luisa Stegat, Elena Laakmann, Sven Peine, Sabine Riethdorf, Volkmar Mueller, Klaus Pantel, Simon A. Joosse
Summary: CTCs have been found to be associated with metastasis and survival in breast cancer patients. Evaluating the expression of estrogen receptors (ER) in CTCs may help with personalized treatment. Some patients have both ER-positive and ER-negative CTCs at the time of metastasis, which may lead to resistance to hormonal therapy. Therefore, monitoring the status of CTC-ER in breast cancer patients can provide prognostic and predictive value.
Article
Microbiology
Olesya Zhuravleva, Galina K. Oleinikova, Alexandr S. Antonov, Natalia N. Kirichuk, Dmitry N. Pelageev, Anton B. Rasin, Alexander S. Menshov, Roman S. Popov, Natalya Yu Kim, Ekaterina A. Chingizova, Artur R. Chingizov, Olga O. Volchkova, Gunhild von Amsberg, Sergey A. Dyshlovoy, Ekaterina A. Yurchenko, Irina Guzhova, Anton N. Yurchenko
Summary: Six new polyketides, acrucipentyns A-F, were isolated from the alga-derived fungus Asteromyces cruciatus KMM 4696. The absolute configuration of acrucipentyn A was determined using modified Mosher's method and ROESY data analysis. Acrucipentyns A-E are the first examples of chlorine-containing asperpentyn-like compounds and exhibit antimicrobial activity.
Editorial Material
Oncology
Simon A. Joosse, Klaus Pantel
Summary: The study discovered that cancer patients had increased levels of cfDNA in their serum compared to healthy individuals, with the highest levels found in patients with metastatic cancer. Changes in serum DNA levels were found to be correlated with treatment outcomes, with increased levels indicating poor response and decreases indicating a better prognosis. Liquid biopsies based on cfDNA have become an important tool for personalized cancer treatment, allowing for early detection, improved staging, real-time monitoring of therapeutic efficacy, and detection of therapeutic targets and resistance mechanisms.
Editorial Material
Medical Laboratory Technology
Simon A. Joosse, Klaus Pantel
CLINICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Sergey A. Dyshlovoy, Sergey N. Fedorov, Vasily Svetashev, Tatiana N. Makarieva, Anatoliy Kalinovsky, Olga P. Moiseenko, Vladimir B. Krasokhin, Larisa K. Shubina, Alla G. Guzii, Gunhild von Amsberg, Valentin A. Stonik
Summary: This study isolated six new AGE-type compounds from a marine sponge and demonstrated their weak cytotoxic activity in various human cancer cells. Furthermore, the results revealed the protective role of ERK2 and JNK1 in the cellular response to AGE-induced cytotoxic effects.
Article
Chemistry, Medicinal
Sergey A. Dyshlovoy, Olesya I. Zhuravleva, Jessica Hauschild, Tobias Busenbender, Dmitry N. Pelageev, Anton N. Yurchenko, Yuliya V. Khudyakova, Alexandr S. Antonov, Markus Graefen, Carsten Bokemeyer, Gunhild von Amsberg
Summary: Marine fungi are a valuable source for new bioactive molecules with various biological activities. In this study, a new indole diketopiperazine alkaloid, deoxy-14,15-dehydroisoaustamide (1), was isolated from the marine-derived fungus Penicillium dimorphosporum KMM 4689 associated with a soft coral. The structure and absolute configuration of this metabolite were determined using HR-MS, 1D and 2D NMR, and CD data. Compound 1 showed no cytotoxicity against human normal and cancer cell lines up to 100 μM, but it resensitized prostate cancer 22Rv1 cells to the androgen receptor (AR) blocker enzalutamide by inducing degradation of the AR transcription variant V7 (AR-V7) and suppressing AR signaling. This isolated alkaloid holds promise as a candidate for combinational therapy of castration resistant prostate cancer, including drug-resistant subtypes.
Review
Biochemistry & Molecular Biology
Alexey S. S. Rzhevskiy, Alina Y. Y. Kapitannikova, Denis V. V. Butnaru, Evgeniy V. V. Shpot, Simon A. A. Joosse, Andrei V. V. Zvyagin, Majid Ebrahimi Warkiani
Summary: Currently, there is a lack of sensitive and specific methods for the detection and prognosis of early stage prostate cancer. Liquid biopsy, a novel technique that extracts biomarkers such as circulating tumor cells, exosomal miRNAs, and circulating DNAs, has been investigated as a supplement or alternative to the traditional screening methods for prostate cancer.
Editorial Material
Chemistry, Medicinal
Sergey A. A. Dyshlovoy, Timofey V. V. Malyarenko, Olesya I. I. Zhuravleva, Hiroshi Tomoda, Maxim E. E. Zhidkov
Article
Chemistry, Medicinal
Oleg A. Tryapkin, Alexey V. Kantemirov, Sergey A. Dyshlovoy, Vladimir S. Prassolov, Pavel V. Spirin, Gunhild von Amsberg, Maria A. Sidorova, Maxim E. Zhidkov
Summary: Fascaplysin is a marine alkaloid that has diverse and potent biological activity, making it a promising lead drug candidate. It has the potential to be an effective anticancer agent due to its ability to inhibit CDK4 and induce intrinsic apoptosis in cancer cells. However, its high toxicity caused by DNA intercalation and the limited methods for synthesis of derivatives hinder the studies on structural optimization.
Article
Medicine, Research & Experimental
Sergey A. Dyshlovoy, Jessica Hauschild, Simone Venz, Christoph Krisp, Katharina Kolbe, Svenja Zapf, Sarina Heinemann, Krystian D. Fita, Larisa K. Shubina, Tatyana N. Makarieva, Alla G. Guzii, Tina Rohlfing, Moritz Kaune, Tobias Busenbender, Thomas Mair, Manuela Moritz, Ekaterina V. Poverennaya, Hartmut Schluter, Volodymyr Serdyuk, Valentin A. Stonik, Judith Dierlamm, Carsten Bokemeyer, Malte Mohme, Manfred Westphal, Katrin Lamszus, Gunhild von Amsberg, Cecile L. Maire
Summary: Rhizochalinin (Rhiz) is a cytotoxic sphingolipid synthesized from the marine compound rhizochalin. It shows high activity against human glioblastoma cell lines, inducing apoptosis, cell cycle arrest, and inhibition of autophagy. Rhiz also enhances the inhibitory effects of γ-irradiation and synergizes with EGFR inhibitors. However, alternative drug delivery methods are needed due to its inability to cross the blood brain barrier.
MOLECULAR PHARMACEUTICS
(2023)