Article
Cell Biology
Taiga Kijima, Daisuke Kurokawa, Yasunori Sasakura, Michio Ogasawara, Satoe Aratake, Kaoru Yoshida, Manabu Yoshida
Summary: This study demonstrates the important role of CatSper in sperm motility in ascidians and mammals, participating in spermatogenesis and basic motility mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kogiku Shiba, Kazuo Inaba
Summary: Spermatozoa can sense and respond to environmental signals to ensure successful fertilization. In the ascidian Ciona intestinalis, CNG channels play a vital role in regulating sperm motility and intracellular calcium regulation during chemotaxis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Evolutionary Biology
Jumpei Ikenaga, Satoe Aratake, Kaoru Yoshida, Manabu Yoshida
Summary: Sperm chemotaxis, an important process in fertilization, involves the attraction of sperms to conspecific eggs via species-specific attractants. This study identifies the plasma membrane-type Ca2+/ATPase (PMCA) as the receptor for the sperm-activating and -attracting factor (SAAF) in the ascidian Ciona intestinalis. The researchers found that PMCA recognizes the species-specific structure of SAAF and its testis-specific C-terminal region is involved in the activation and chemotaxis of ascidian sperms.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Noburu Sensui, Yosinori Itoh, Nobuhiko Okura, Kogiku Shiba, Shoji A. Baba, Kazuo Inaba, Manabu Yoshida
Summary: In Phlebobranchiata ascidians, the acidic conditions in the gonoductal fluids keep the gametes infertile, especially the eggs, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increases fertilization rates, even under low pH conditions. An increase in ambient pH triggers the release of sperm attractants from the egg and is necessary for sperm chemotaxis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maki Kimura, Hiroyuki Mochizuki, Ryouichi Satou, Miyu Iwasaki, Eitoyo Kokubu, Kyosuke Kono, Sachie Nomura, Takeshi Sakurai, Hidetaka Kuroda, Yoshiyuki Shibukawa
Summary: The study revealed the crucial role of PMCA in maintaining calcium homeostasis and promoting mineralization in odontoblasts, especially in the Ca2+ extrusion pathway under hypotonic and alkaline stimulation. Additionally, PMCA is involved in dentinogenesis induced by mechanical or direct alkaline stimulation in both physiological and pathological conditions.
Article
Multidisciplinary Sciences
Maoxing Zhang, Yin Wang, Xi Chen, Feiyun Xu, Ming Ding, Wenxiu Ye, Yuya Kawai, Yosuke Toda, Yuki Hayashi, Takamasa Suzuki, Houqing Zeng, Liang Xiao, Xin Xiao, Jin Xu, Shiwei Guo, Feng Yan, Qirong Shen, Guohua Xu, Toshinori Kinoshita, Yiyong Zhu
Summary: The study demonstrates that overexpression of a single gene, OSA1, in rice can enhance nitrogen and carbon utilization efficiency, leading to a significant increase in grain yield.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Durva Panchal, Shweta Bhagwat, Priyanka Parte
Summary: This study found that N-formyl-L-aspartate (NFA) is a chemoattractant for sperm, and the chemotactic response of sperm to NFA is mediated through the beta-2-adrenergic receptor (beta-2-AR). NFA increases intracellular Ca2+ concentration but decreases cAMP levels in sperm. However, NFA does not induce sperm capacitation or affect the acrosome structure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Horticulture
Lingjie Chen, Ran Zhao, Jie Yu, Jiajia Gu, Yongqiang Li, Wenrong Chen, Weidong Guo
Summary: Blueberries prefer acid soils, and this study found that their root activity and expression of VcHA genes were highest at pH 5.0. Overexpressing VcHA genes in Arabidopsis resulted in increased root activity and plant growth. Among the VcHA genes, VcHA10.2 and VcHA5 showed the strongest ability to pump H+.
SCIENTIA HORTICULTURAE
(2022)
Article
Reproductive Biology
Naomi C. Bernecic, Simon P. de Graaf, Tamara Leahy, Bart M. Gadella
Summary: This study reveals that both sheep serum and HDLs can induce cholesterol efflux in ram spermatozoa, with HDLs also triggering hyperactivated motility. Inhibition of ABCA1 and SR-BI function only marginally reduces cholesterol efflux, suggesting a specific facilitated pathway for cholesterol efflux in ram spermatozoa. These findings shed light on the complexity of events leading to fertilization and suggest the potential application of HDL supplementation in IVF media.
BIOLOGY OF REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Ronja Schiemann, Annika Buhr, Eva Cordes, Stefan Walter, Jurgen J. Heinisch, Paola Ferrero, Hendrik Milting, Achim Paululat, Heiko Meyer
Summary: In this study, the researchers found that the endopeptidase Neprilysin 4 plays an essential role in regulating SERCA and maintaining Ca2+ homeostasis in cardiomyocytes, thus contributing to proper heart function.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Zhan Li, Jeffrey F. Harper, Chrystle Weigand, Jian Hua
Summary: Calmodulin-regulated Ca2+ pumps play a crucial role in maintaining basal cytosol Ca2+ levels, which are important for biotic and abiotic stress responses in Arabidopsis. The resting cytosol Ca2+ levels have an impact on plant survival under chilling and heat-stress conditions, and the regulation of these levels is critical for plant immune activation and environmental responses. Loss-of-function mutants of the calmodulin-regulated Ca2+ pumps exhibited elevated resting cytosol Ca2+ levels and increased susceptibility to chilling and heat stress, indicating the importance of these pumps in plant stress tolerance.
Article
Oncology
Randa Naffa, Rita Padanyi, Attila Ignacz, Zoltan Hegyi, Balint Jezso, Sarolta Toth, Karolina Varga, Laszlo Homolya, Luca Hegedus, Katalin Schlett, Agnes Enyedi
Summary: Our study demonstrates that PMCA4b inhibits migration and metastatic activity of melanoma cells by affecting F-actin rearrangement within the cells and regulating Ca2+ concentration levels at specific plasma membrane locations which play a critical role in maintaining cell polarity and motility.
Article
Biochemistry & Molecular Biology
Maja Radulovic, Eva Maria Wenzel, Sania Gilani, Lya K. K. Holland, Alf Hakon Lystad, Santosh Phuyal, Vesa M. Olkkonen, Andreas Brech, Marja Jaattela, Kenji Maeda, Camilla Raiborg, Harald Stenmark
Summary: This study reveals an additional mechanism for lysosome repair that is activated independently of the ESCRT machinery. It shows that damaged lysosomes rapidly form contacts with the endoplasmic reticulum (ER), mediated by ER proteins VAPA/B, which provide lipids for membrane repair. The study also highlights the importance of lysosomal phosphatidylserine and cholesterol accumulation, as well as the generation and removal of PtdIns4P in this repair response.
Article
Plant Sciences
Bo Yu, Ying Sun, Xinkai Jin, Zizhao Xie, Xingxing Li, Junli Huang
Summary: Understanding how plants determine root directional growth towards nutrient sources is crucial for optimizing plant growth. This study discovered that a rice Ca2+-permeable channel, OsGLR3.4, specifically modulates root growth and tropism towards glycine (Gly) via Ca2+-mediated electrical activity and reactive oxygen species (ROS) production. OsGLR3.4 was found to be necessary for root nutritropism growth towards Gly by affecting the distribution of membrane depolarization and ROS across the root tip. Additionally, OsGLR3.4 promotes nitrate uptake in roots, indicating its potential roles in crop yield.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Shweta B. Borkar, Manorma Negi, Neha Kaushik, Shaik Abdul Munnaf, Linh Nhat Nguyen, Eun Ha Choi, Nagendra Kumar Kaushik
Summary: The proportion of resistant bacteria will increase over time, which is a major concern. Effective and biocompatible therapeutic strategies are urgently needed to combat these bacteria. Non-thermal plasma has been extensively studied for its antibacterial activity. This study investigates the inactivation efficiency and mechanisms of plasma-generated nitric oxide water (PG-NOW) on pathogenic Gram-negative and Gram-positive bacteria in water, air, soil, and food. Results showed that PG-NOW treatment effectively inhibits bacterial growth and accumulates nitric oxide within the cells. Gene expressions related to nitrosative stress and oxidative stress were also analyzed, supporting the nitrosative effect of PG-NOW. Additionally, changes in cell morphology were observed. These findings provide evidence for the further development of PG-NOW-based antibacterial treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Biophysics
Hiroki Arima, Hidekazu Tsutsui, Kohei Takeshita, Atsushi Nakagawa, Ayako Sakamoto, Manabu Yoshida, Yasushi Okamura
BIOPHYSICAL JOURNAL
(2016)
Article
Reproductive Biology
V. Gallego, M. Yoshida, D. Kurokawa, J. F. Asturiano, G. J. Fraser
Article
Biochemistry & Molecular Biology
Hiroki Arima, Hidekazu Tsutsui, Ayako Sakamoto, Manabu Yoshida, Yasushi Okamura
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Plant Sciences
Tomohiro Watanabe, Hajime Shibata, Makoto Ebine, Hiroshi Tsuchikawa, Nobuaki Matsumori, Michio Murata, Manabu Yoshida, Masaaki Morisawa, Shu Lin, Kosei Yamauchi, Ken Sakai, Tohru Oishi
JOURNAL OF NATURAL PRODUCTS
(2018)
Article
Zoology
Jumpei Ikenaga, Natsumi Hookabe, Hisanori Kohtsuka, Manabu Yoshida, Hiroshi Kajihara
ZOOLOGICAL SCIENCE
(2019)
Article
Cell Biology
Nana Kinoshita-Terauchi, Kogiku Shiba, Makoto Terauchi, Francisco Romero, Hector Vincente Ramirez-Gomez, Manabu Yoshida, Taizo Motomura, Hiroshi Kawai, Takuya Nishigaki
Article
Biochemistry & Molecular Biology
Miyuki Shindo, Masafumi Inui, Woojin Kang, Moe Tamano, Cai Tingwei, Shuji Takada, Taku Hibino, Manabu Yoshida, Kaoru Yoshida, Hiroshi Okada, Teruaki Iwamoto, Kenji Miyado, Natsuko Kawano
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Daiki Sakaguchi, Kenji Miyado, Teruaki Iwamoto, Hiroshi Okada, Kaoru Yoshida, Woojin Kang, Miki Suzuki, Manabu Yoshida, Natsuko Kawano
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Zoology
Jumpei Ikenaga, Hiroshi Kajihara, Manabu Yoshida
Summary: The purple color variant corresponds to the true Kulikovia alborostrata, while the brown-yellow variant corresponds to Lineus fulvus. These two species are reproductively isolated from each other by prezygotic mechanisms.
ZOOLOGICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Vitaliy Kholodnyy, Borys Dzyuba, Marek Rodina, Hermes Bloomfield-Gadelha, Manabu Yoshida, Jacky Cosson, Sergii Boryshpolets
Summary: The fertilization of freshwater fish can be negatively affected by environmental factors, with egg and ovarian fluid playing a significant role in triggering gamete encounters. The ovarian fluid of rainbow trout affects sperm performance by inducing a chemotaxis-like effect on activated male gametes, creating an optimal environment for sperm performance. The individual interaction between egg (ovarian fluid) and sperm reflects specific features of the spawning process in this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Kazunori Morohoshi, Takeo Yamazaki, Keiji Kito, Ban Sato, Woojin Kang, Taku Hibino, Manabu Yoshida, Kaoru Yoshida, Teruaki Iwamoto, Mitsutoshi Yamada, Kenji Miyado, Natsuko Kawano
Summary: In both men and women, pathogenic bacteria can enter the reproductive tract and cause harmful symptoms. Even in the absence of the majority of seminal proteins, antibacterial activity remains in the seminal plasma. Prostate and testis express a novel antibacterial protein, the sequence of which is broadly conserved across mammals.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2021)
Article
Evolutionary Biology
Jumpei Ikenaga, Satoe Aratake, Kaoru Yoshida, Manabu Yoshida
Summary: Sperm chemotaxis, an important process in fertilization, involves the attraction of sperms to conspecific eggs via species-specific attractants. This study identifies the plasma membrane-type Ca2+/ATPase (PMCA) as the receptor for the sperm-activating and -attracting factor (SAAF) in the ascidian Ciona intestinalis. The researchers found that PMCA recognizes the species-specific structure of SAAF and its testis-specific C-terminal region is involved in the activation and chemotaxis of ascidian sperms.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Noburu Sensui, Yosinori Itoh, Nobuhiko Okura, Kogiku Shiba, Shoji A. Baba, Kazuo Inaba, Manabu Yoshida
Summary: In Phlebobranchiata ascidians, the acidic conditions in the gonoductal fluids keep the gametes infertile, especially the eggs, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increases fertilization rates, even under low pH conditions. An increase in ambient pH triggers the release of sperm attractants from the egg and is necessary for sperm chemotaxis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Naoya Araki, Natsuko Kawano, Woojin Kang, Kenji Miyado, Kaoru Yoshida, Manabu Yoshida