Review
Materials Science, Multidisciplinary
Xi Jiang, Nitash P. Balsara
Summary: Electron microscopy has been essential in characterizing polymers, with recent developments allowing for atomic resolution imaging of polymer crystals and providing a comprehensive understanding of strategies towards high-resolution imaging of radiation-sensitive polymer materials at different length scales.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Geochemistry & Geophysics
R. Gerrits, R. Wirth, A. Schreiber, I Feldmann, N. Knabe, J. Schott, L. G. Benning, A. A. Gorbushina
Summary: Many microorganisms, including fungi, weather minerals through the formation of biofilms on their surfaces. Experimental results suggest that the specific traits of fungal biofilms have minimal impact on mineral weathering.
Article
Environmental Sciences
Alix Guenther, Anne Wollenberg, Manja Vogel, Bjoern Drobot, Robin Steudtner, Leander Freitag, Rene Huebner, Thorsten Stumpf, Johannes Raff
Summary: This study investigated the interaction of fungi with Eu(III), with S. commune showing higher binding capacity. The metal could be immobilized on the cell surface, in cell membranes, and within organelle membranes. The phosphate groups of organic ligands were identified as important functional groups for binding Eu(III).
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Priti Kharel, Blanka E. Janicek, Sang Hyun Bae, Amanda L. Loutris, Patrick T. Carmichael, Pinshane Y. Huang
Summary: Here, we demonstrate atomic-resolution scanning transmission electron microscopy (STEM) imaging of light elements in small organic molecules on graphene. Our approach involves low-dose, room-temperature, aberration-corrected STEM to create high-quality composite images, with sufficient resolution to distinguish individual carbon and nitrogen atoms.
Article
Chemistry, Analytical
Olivier De Castro, Jean-Nicolas Audinot, Hung Quang Hoang, Cherif Coulbary, Olivier Bouton, Rachid Barrahma, Alexander Ost, Charlotte Stoffels, Chengge Jiao, Mikhail Dutka, Michal Geryk, Tom Wirtz
Summary: The article introduces the use of magnetic sector secondary ion mass spectrometry (SIMS) on a widely used instrument, focused ion beam (FIB)-scanning electron microscopy (SEM), for nanoscale investigations. It provides a detailed description of the instrument's layout and performance, as well as the research results obtained in various fields. The system demonstrates versatile capability for high-performance correlative studies in materials science and life sciences.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hsueh-Ying Chen, C. Blake Wilson, Robert Tycko
Summary: The spatial resolution of MRI is limited by signal detection sensitivity, but can be improved by performing measurements at low temperature and using dynamic nuclear polarization (DNP) technique. This allows for higher resolution and shorter data acquisition times, offering a promising direction for high-resolution MRI studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biology
Michela Relucenti, Giuseppe Familiari, Orlando Donfrancesco, Maurizio Taurino, Xiaobo Li, Rui Chen, Marco Artini, Rosanna Papa, Laura Selan
Summary: Bacterial biofilms cause infections that are often resistant to antibiotics, necessitating detailed imaging techniques for research. Scanning electron microscopy with customized protocols is preferred for ultrastructural characterization of biofilm matrix and bacterial cells in drug treatment studies, providing unparalleled image quality and resolution. Various imaging methodologies, including traditional and innovative scanning electron microscopy techniques, have been used in biofilm studies to deepen understanding of their structure.
Article
Nanoscience & Nanotechnology
Bin Meng, Yong Xie, Le Chen, Haitao Wang, Ming Li, Zhuxin Dong
Summary: This paper presents a convenient technical scheme for enhancing the performance of atomic force microscopy (AFM) tips in tip-enhanced Raman spectroscopy (TERS) applications. The silver decoration on homemade tips is confined to the apex of commercial tips through an AFM-controlled electrochemical reaction. The efficiency of the metal coating is ensured by the reduction of Ag+ in a highly sealed environment. Key factors affecting the process include the use of silver nitrate solution, ambient relative humidity and temperature, electric potential bias, and tip-surface distance. The silver-coated tips are then tested for TERS measurement of carbon nanotubes (CNTs), resulting in simultaneous characterization of the CNTs' morphology and chemical properties. The Raman spectra show that our plasmonic tip exhibits a local field signal increase of approximately 30-fold, and the corresponding TERS image can resolve at the single-pixel level laterally.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Patrick Fiorenza, Mario S. Alessandrino, Beatrice Carbone, Alfio Russo, Fabrizio Roccaforte, Filippo Giannazzo
Summary: In this paper, a two-dimensional planar scanning capacitance microscopy (SCM) method was used to visualize the channel region of large-area 4H-SiC power MOSFETs with high spatial resolution and estimate the homogeneity of the channel length over the entire device perimeter. The method allowed for observing fluctuations in the channel geometry under different processing conditions, as well as elucidating the impact of ion implantation parameters on the channel.
Article
Multidisciplinary Sciences
Marie Vancova, Radim Skoupy, Eva Durinova, Tomas Bily, Jana Nebesarova, Vladislav Krzyzanek, Ales Kolouch, Petr Horodysky
Summary: This study reports the first use of two live-cell imaging agents as cathodoluminescent probes. The approach allows for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Xuehua Wang, Junping Zhong, Mingyi Wang, Honglian Xiong, Dingan Han, Yaguang Zeng, Haiying He, Haishu Tan
Summary: A new SOFI algorithm is proposed in this study, which calculates n orders covariance for each pixel to achieve almost 2n-fold resolution improvement. An optimized deconvolution method is also introduced to further enhance the resolution and suppress noise generation. The algorithm successfully increases the temporal-spatial resolution of SOFI, while preserving the sample's structure, achieving a resolution of 58 nm for 20 experimental images with an acquisition time of 0.8 seconds.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Chemistry, Multidisciplinary
Fang Yuan, Yanyu Jia, Guangming Cheng, Ratnadwip Singha, Shiming Lei, Nan Yao, Sanfeng Wu, Leslie M. Schoop
Summary: We develop a robust method for atomic-resolution visualization of monolayers and twisted-bilayer WTe2 (tWTe(2)), confirming their high crystalline quality and structural stability. Our findings provide important experimental information for modeling the physical properties of 2D WTe2 systems.
Article
Multidisciplinary Sciences
Kathryn A. Whitehead, Ted Deisenroth, Andrea Preuss, Christopher M. Liauw, Joanna Verran
Summary: Microbial biofouling can lead to deterioration, cracking, and fracturing of polymer surfaces. Adhesion strength of fungal spores varies on different surfaces. Spore attachment is a multifactorial process influenced by surface and microbial factors.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yeon Ui Lee, Shilong Li, G. Bimananda M. Wisna, Junxiang Zhao, Yuan Zeng, Andrea R. Tao, Zhaowei Liu
Summary: The authors demonstrate a label-free superresolution imaging method using a hyperbolic material for tailored light-matter interactions. By enhancing scattering and utilizing dark-field detection, the resolution is improved by 5.5 times beyond the diffraction limit. This method provides capabilities for super-resolution imaging where fluorescence is not available or challenging to apply.
NATURE COMMUNICATIONS
(2022)
Article
Optics
Weisong Zhao, Shiqun Zhao, Zhenqian Han, Xiangyan Ding, Guangwei Hu, Liying Qu, Yuanyuan Huang, Xinwei Wang, Heng Mao, Yaming Jiu, Ying Hu, Jiubin Tan, Xumin Ding, Liangyi Chen, Changliang Guo, Haoyu Li
Summary: High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision profiling in biomedical applications. The proposed SACD method reduces the number of frames required for SR imaging and enables four-dimensional imaging of live cells and events. SACD improves accessibility to SR imaging and facilitates high-throughput and low-cost biological studies.
Article
Polymer Science
S. Dhanavel, T. A. Revathy, T. Sivaranjani, K. Sivakumar, P. Palani, V. Narayanan, A. Stephen
Article
Biochemistry & Molecular Biology
Antony Stalin, Subramani Kandhasamy, Balakrishnan Senthamarai Kannan, Rama Shanker Verma, Savarimuthu Ignacimuthu, Yrjala Kim, Shao Qingsong, Yuan Chen, Perumal Palani
BIOORGANIC CHEMISTRY
(2020)
Correction
Plant Sciences
M. K. Prasannakumar, P. Buela Parivallal, C. Manjunatha, H. B. Mahesh, D. Pramesh, Karthik S. Narayan, Venkatesh Babu Gopal, K. Priyanka, M. E. Puneeth, K. T. Rangaswamy
AUSTRALASIAN PLANT PATHOLOGY
(2020)
Article
Polymer Science
Prashanna Suvaitha Sundara Selvam, Gandhi Shree Chinnadurai, Dhinagaran Ganesan, Palani Perumal, Venkatachalam Kandan
Summary: This study analyzed the antimicrobial activity of CdO/ZnO-ESM nanocomposites synthesized using a unique method involving eggshell membrane as a bio-template. The nanocomposites showed exceptional antimicrobial activity against both Gram-positive and Gram-negative bacteria, with a maximum inhibition zone of 24 mm observed in Salmonella enterica.
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
S. Dhanavel, T. Sivaranjani, K. Sivakumar, P. Palani, Vinod Kumar Gupta, V Narayanan, A. Stephen
Summary: This study utilized a novel nanocomposite to load curcumin and 5-fluorouracil for effective treatment of colorectal cancer cells, overcoming multidrug resistance and demonstrating synergistic anticancer activity.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Sivakumar Krishnan, Gandhi Shree Chinnadurai, Kartik Ravishankar, Dhamodharan Raghavachari, Palani Perumal
Summary: This study identified a strain, Isotpericola variabilis, with relatively high PHB production and optimized the production conditions for potential biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
M. K. Prasannakumar, Buela P. Parivallal, Chennappa Manjunatha, Devanna Pramesh, Karthik S. Narayan, Gopal Venkatesh, Sahana N. Banakar, H. B. Mahesh, Ramu S. Vemanna, K. T. Rangaswamy
Summary: This study developed a simple, specific, sensitive, robust, and cost-effective LAMP assay for foreground selection of seven BLB resistance (R) genes in resource-poor laboratory settings.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Sivakumar Krishnan, Gandhi Shree Chinnadurai, Kartik Ravishankar, Dhamodharan Raghavachari, Palani Perumal
Summary: Recent investigations have revealed the potential usage of oil cake as a cost-effective carbon source for commercial production of PHB by Bacillus sp., showing promise in environmental and economic aspects.
Article
Microbiology
Siddulakshmi Prasanna, M. K. Prasannakumar, H. B. Mahesh, Gopal Venkatesh Babu, P. Kirnaymayee, M. E. Puneeth, Karthik S. Narayan, D. Pramesh
Summary: This study investigated the bio-macromolecules of Bacillus velezensis strains A6 and P42 and their potential application in controlling plant pathogens. Various techniques were used to analyze the bioactive compounds produced by these strains, revealing their inhibitory effects on rice and pomegranate pathogens.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Malairaj Sathuvan, Ramar Thangam, Gopal Venkateshbabu, Kit-Leong Cheong, Heemin Kang, Yang Liu
Summary: The purification of R-phycoerythrin from Gracilaria corticata was efficiently achieved using a single-step chromatographic method based on polyacrylamide gel electrophoresis, maintaining structural integrity, UV-Vis spectrum characteristics, and stability. In vitro studies demonstrated the effective cell imaging properties of purified R-PE without impacting cell proliferation in Vero and Hep-2 cell lines.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
P. Sridhar, G. Venkateshbabu, D. Hemalakshmi, V. M. Kirthika, P. Palani
Summary: Distinct symptoms of necrosis of leaves and severe rotting of roots were observed in groundnut plants in an agricultural field in Arakkonam, Tamil Nadu, India. The pathogenic fungus Marasmius palmivorus was isolated and identified based on morphological characteristics and nucleotide sequencing analysis. The fungus showed pathogenicity when inoculated on healthy groundnut plants, confirming it as the causal agent of white root rot disease in groundnut.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
N. Kavya, M. K. Prasannakumar, Gopal Venkateshbabu, Vidya Niranjan, Akshay Uttarkar, P. Buela Parivallal, Sahana N. Banakar, H. B. Mahesh, Pramesh Devanna, K. G. Manasa, Tagginahalli N. Shivakumara
Summary: In this study, seven novel effectors in Rhizoctonia solani were identified and their functions, reactivity, and stability were evaluated. Target proteins involved in the regulation of rice defense mechanisms were also identified. These findings have implications for the management of sheath blight in rice.
Article
Biotechnology & Applied Microbiology
B. S. Chandrashekar, M. K. Prasannakumar, Gopal Venkateshbabu, H. B. Mahesh, M. E. Puneeth, Karthik S. Narayan, P. Buela Parivallal, D. Pramesh, Sahana N. Banakar, Swati S. Patil
Summary: Bacterial soft rot in carrots is a devastating disease, and in this study, eight isolates of the bacterial pathogen causing soft rot in carrots were collected from India. The pathogen was identified as Klebsiella variicola through 16S rRNA sequencing, and its morphological characteristics were investigated under scanning electron microscopy. Two novel strains of Bacillus velezensis (A6 and P42) were found to have strong antagonistic activity against K. variicola, producing bioactive compounds with antibacterial and antifungal properties. These strains can be used as biocontrol agents to manage soft rot diseases of carrot under field conditions.
LETTERS IN APPLIED MICROBIOLOGY
(2023)