Article
Food Science & Technology
Olga V. Averina, Aleksey S. Kovtun, Dilara A. Mavletova, Rustam H. Ziganshin, Valery N. Danilenko, Dasha Mihaylova, Denica Blazheva, Aleksandar Slavchev, Mariya Brazkova, Salam A. Ibrahim, Albert Krastanov
Summary: This study provides insights into the mechanism of oxidative stress response in B. longum and factors contributing to its survival in functional food products through transcriptomic, proteomic, and metabolomic analyses.
Article
Biochemistry & Molecular Biology
Kurtis F. Budden, Shaan L. Gellatly, Annalicia Vaughan, Nadia Amorim, Jay C. Horvat, Nicole G. Hansbro, David L. A. Wood, Philip Hugenholtz, Paul G. Dennis, Peter A. B. Wark, Philip M. Hansbro
Summary: In this study, it was found that regardless of its acetate-producing capacity, Bifidobacterium longum subsp. longum alleviated cigarette smoke-induced inflammation, reduced inflammatory cytokine expression and adhesion factor expression, and improved the depletion of cecal butyrate levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Monica F. Quintanilha, Vivian C. Miranda, Ramon O. Souza, Bruno Gallotti, Clenio Cruz, Elandia A. Santos, Jacqueline Alvarez-Leite, Luis C. L. Jesus, Vasco Azevedo, Luisa M. Trindade, Valbert N. Cardoso, Enio Ferreira, Barbara A. Carvalho, Pedro M. G. Soares, Angelica T. Vieira, Jacques R. Nicoli, Flaviano S. Martins
Summary: This study demonstrates that Bifidobacterium longum 5(1A), a probiotic, has a protective effect against intestinal damage caused by irinotecan, suggesting its potential use as a probiotic therapy for mucositis.
Article
Microbiology
Romina Diaz, Alexis Torres-Miranda, Guillermo Orellana, Daniel Garrido
Summary: This study compares genomic data of Bifidobacterium longum subsp. longum from different sources, revealing variable adaptations to carbohydrate consumption and the presence of glycosyl hydrolases in certain infant and adult genomes for human milk oligosaccharide use. Predictive genomic analysis demonstrates the potential of B. longum M12 to utilize fucosylated HMOs.
Article
Biotechnology & Applied Microbiology
Masahiro Komeno, Yuki Yoshihara, Junya Kawasaki, Wataru Nabeshima, Koshi Maeda, Yuki Sasaki, Kiyotaka Fujita, Hisashi Ashida
Summary: In this study, the degradation pathways of AX and AXOs by Bifidobacterium longum subsp. longum were characterized, and the crucial roles of BlArafD and BlArafE in the assimilation of AX were identified.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Li Zhao, Qinggang Xie, Smith Etareri Evivie, Yingxue Yue, Han Yang, Xiuli Lv, Fei Liu, Bailiang Li, Guicheng Huo
Summary: B. longum subsp. longum K5 has anti-inflammatory and intestinal barrier protective effects. It can regulate inflammatory mediators and improve cell paracellular permeability. K5 performs better than K15 in these aspects. The whole-genome sequencing results reveal genes associated with the immune system, carbohydrate metabolism, and signal transduction in K5-specific genes.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Xingyuan Zhang, Jing Gong, Wenyi Huang, Wen Liu, Chong Ma, Rongyao Liang, Ye Chen, Zhiyong Xie, Pei Li, Qiongfeng Liao
Summary: Researchers successfully isolated a new exopolysaccharide, S-EPS-1, from the strain Bifidobacterium longum subsp. longum XZ01 (BLSL1), and analyzed its structural features. S-EPS-1 exhibited immunoregulatory and antioxidant activities, suggesting its potential application as a functional food.
Article
Gastroenterology & Hepatology
Xiaomeng You, Asha Rani, Ezgi Ozcan, Yang Lyu, David A. Sela
Summary: Human milk shapes the microbiota in the infant gut, and urea in milk may serve as a nitrogen source for the microbiota. Bifidobacterium infantis strains are capable of utilizing urea nitrogen and play a role in infant nutrition and development. Isotopically labeled urea nitrogen can be incorporated into microbial products for infant host utilization.
Article
Food Science & Technology
Jiaqi Guan, Fei Liu, Sijia Zhao, Smith Etareri Evivie, Jialu Shi, Na Li, Li Zhao, Yingxue Yue, Qinggang Xie, Guicheng Huo, Bailiang Li
Summary: Bifidobacterium longum subsp. longum strain K2-21-4 can promote CCD841 CoN cell proliferation, accelerate the cell cycle process, decrease cell apoptosis, and activate proteins related to tight junctions. These findings suggest that B. longum K2-21-4 can regulate intestinal development by promoting cell proliferation and accelerating the maturation of the internal barrier.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biotechnology & Applied Microbiology
Spencer S. Santos, Vivian C. Miranda, Luisa M. Trindade, Valbert N. Cardoso, Diego C. Reis, Geovanni D. Cassali, Jacques R. Nicoli, Denise C. Cara, Flaviano S. Martins
Summary: This study evaluated the probiotic effect of Bifidobacterium longum subsp. longum 5(1A) in a murine model of food allergy. The results showed that oral administration of this probiotic prevented weight loss, reduced immune reactions, and improved intestinal permeability. The dose of 10^8 CFU showed the best protective effect, and the use of live bacteria was necessary to achieve these beneficial effects.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Li Zhao, Song Wang, Jiahuan Dong, Jialu Shi, Jiaqi Guan, Deyu Liu, Fei Liu, Bailiang Li, Guicheng Huo
Summary: This study isolated and screened B. longum subsp. longum strains with potential antioxidant properties from the gut microbiota of infants, with strain K5 showing high antioxidant capacity and promoting intestinal epithelial cell proliferation. These findings suggest that B. longum subsp. longum K5 could be a probiotic candidate for infant nutrition.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yingxue Yue, Yuqi Wang, Rui Yang, Deyu Liu, Yang Cheng, Shuang Li, Lenan Sun, Bailiang Li, Guicheng Huo
Summary: The exopolysaccharides produced by B. infantis E4 have immunostimulatory and anti-inflammatory activities, suggesting their potential as a prebiotic for maintaining future health.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Multidisciplinary Sciences
Kai Zhu, Li-Tao Yang, Cheng-Xi Li, Prakash Lakshmanan, Yong-Xiu Xing, Yang-Rui Li
Summary: The study analyzed transcriptomes of healthy and Lxx-infected sugarcane stalks and leaves to reveal sugarcane response to Lxx infection, highlighting alterations in metabolic pathways and defense mechanisms. The research also indicated that inhibition of photosynthesis and changes in gibberellin response contribute to growth retardation in RSD-affected plants.
Article
Biochemistry & Molecular Biology
Shengjie Li, Aoxiang Zhuge, Jiafeng Xia, Shuting Wang, Longxian Lv, Kaicen Wang, Huiyong Jiang, Ren Yan, Liya Yang, Xiaoyuan Bian, Xianwan Jiang, Qiangqiang Wang, Shengyi Han, Lanjuan Li
Summary: This study found that Bifidobacterium longum R0175 can alleviate APAP-induced liver injury by activating the Nrf2 pathway. The gut microbiome plays a crucial role in this process. B. longum R0175 can reduce inflammation, oxidative stress levels, hepatocyte death, and improve microbiome dysbiosis induced by APAP.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Food Science & Technology
Ting Zhang, Zongmin Liu, Jianxin Zhao, Hao Zhang, Wei Chen, Haitao Li, Wenwei Lu, Yue Xiao, Xiuwen Zhou
Summary: This study investigated the biofilm formation mechanism of Bifidobacterium longum using comparative genes, transcriptome, and metabolome analyses. The results revealed that B. longum regulated biofilm formation through AI-2/QS, cAMP, and LuxC/LuxE TCS system, which involved the regulation of Tad IV pilin and extracellular substances secretion. These findings provide insights into the formation mechanism of Bifidobacterium biofilm on food-grade particles.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Sara Sigurlasdottir, Gabriela M. Wassing, Fanglei Zuo, Melanie Arts, Ann-Beth Jonsson
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Nutrition & Dietetics
Rui Yu, Fanglei Zuo, Huiqin Ma, Shangwu Chen
Article
Biotechnology & Applied Microbiology
Zhu Zeng, Fanglei Zu, Harold Marcotte
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Review
Biotechnology & Applied Microbiology
Fanglei Zuo, Shangwu Chen, Harold Marcotte
BIOTECHNOLOGY ADVANCES
(2020)
Review
Biochemical Research Methods
Fanglei Zuo, Harold Marcotte
Summary: Efficient genome editing will enhance the understanding of probiotics' physiological properties and interactions with host and microbiota, leading to the development of next-generation designer probiotics with improved robustness and tailored functionalities.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Immunology
Sara Sigurlasdottir, Kenny Lidberg, Fanglei Zuo, Jane Newcombe, Johnjoe McFadden, Ann-Beth Jonsson
Summary: This study demonstrates that colonization of Neisseria meningitidis in the upper respiratory tract epithelium is crucial for disease development. Dispersal induced by lactate is dependent on bacterial concentration, PilT-dependent Tfp retraction, and protein synthesis, and is influenced by environmental temperature.
INFECTION AND IMMUNITY
(2021)
Article
Allergy
Sophia Bjorkander, Likun Du, Fanglei Zuo, Sandra Ekstrom, Yating Wang, Hui Wan, Natalia Sherina, Lisanne Schoutens, Juni Andrell, Niklas Andersson, Antonios Georgelis, Anna Bergstrom, Harold Marcotte, Inger Kull, Lennart Hammarstrom, Erik Melen, Qiang Pan-Hammarstrom
Summary: This population-based study explored the humoral and cellular immunity to SARS-CoV-2 in young adults and the characteristics of COVID-19 disease. The study found that 28.4% of young adults had seropositivity to SARS-CoV-2, and a portion of them were asymptomatic. The use of public transport was associated with seropositivity, but factors such as sex, asthma, rhinitis, smoking, or body mass index were not. A subset of participants had detectable B-cell and T-cell responses to SARS-CoV-2, indicating immune memory.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Immunology
Hassan Abolhassani, Ahmad Vosughimotlagh, Takaki Asano, Nils Landegren, Bertrand Boisson, Samaneh Delavari, Paul Bastard, Maribel Aranda-Guillen, Yating Wang, Fanglei Zuo, Fabian Sardh, Harold Marcotte, Likun Du, Shen-Ying Zhang, Qian Zhang, Nima Rezaei, Olle Kampe, Jean-Laurent Casanova, Lennart Hammarstrom, Qiang Pan-Hammarstrom
Summary: This study identified a unique case of a patient with a preexisting inborn error of immunity (IEI) who developed critical COVID-19 pneumonia. Genetic analysis revealed homozygous deleterious mutations in the ATM gene and a hemizygous deleterious mutation in the TLR7 gene, underlying Ataxia-Telangiectasia (A-T) and critical COVID-19 in this patient. Among other A-T patients evaluated, SARS-CoV-2 infection resulted in mild symptoms or asymptomatic cases, highlighting the importance of genetic factors in COVID-19 severity.
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)
Article
Immunology
Hassan Abolhassani, Nils Landegren, Paul Bastard, Marie Materna, Mohammadreza Modaresi, Likun Du, Maribel Aranda-Guillen, Fabian Sardh, Fanglei Zuo, Peng Zhang, Harold Marcotte, Nico Marr, Taushif Khan, Manar Ata, Fatima Al-Ali, Remi Pescarmona, Alexandre Belot, Vivien Beziat, Qian Zhang, Jean-Laurent Casanova, Olle Kampe, Shen-Ying Zhang, Lennart Hammarstrom, Qiang Pan-Hammarstrom
Summary: This study identified a rare case with concomitant critical COVID-19 pneumonia and MIS-C, and found a genetic variant associated with IFNAR1 deficiency through whole exome sequencing. It highlights the importance of type I interferon immunity and suggests that inherited or acquired dysregulation of this immune response may contribute to MIS-C.
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Harold Marcotte, Antonio Piralla, Fanglei Zuo, Likun Du, Irene Cassaniti, Hui Wan, Makiko Kumagai-Braesh, Juni Andrell, Elena Percivalle, Jose Camilla Sammartino, Yating Wang, Stelios Vlachiotis, Janine Attevall, Federica Bergami, Alessandro Ferrari, Marta Colaneri, Marco Vecchia, Margherita Sambo, Valentina Zuccaro, Erika Asperges, Raffaele Bruno, Tiberio Oggionni, Federica Meloni, Hassan Abolhassani, Federico Bertoglio, Maren Schubert, Luigi Calzolai, Luca Varani, Michael Hust, Yintong Xue, Lennart Hammarstrom, Fausto Baldanti, Qiang Pan-Hammarstrom
Summary: This study monitored the immune response in COVID-19 patients up to 15 months after symptom onset. It found that the IgG antibody response and plasma neutralizing titers gradually decreased over time but stabilized after 6 months. SARS-CoV-2-specific memory B and T cells persisted in the majority of patients up to 15 months, but there was a significant decrease in specific T cells between 6 and 15 months. Some variants of concern may partially escape the neutralizing activity of plasma antibodies.
Article
Medicine, General & Internal
Maren Schubert, Federico Bertoglio, Stephan Steinke, Philip Alexander Heine, Mario Alberto Ynga-Durand, Henrike Maass, Jose Camilla Sammartino, Irene Cassaniti, Fanglei Zuo, Likun Du, Janin Korn, Marko Milosevic, Esther Veronika Wenzel, Fran Krstanovic, Saskia Polten, Marina Pribanic-Matesic, Ilija Brizic, Fausto Baldanti, Lennart Hammarstrom, Stefan Duebel, Alan Sustic, Harold Marcotte, Monika Strengert, Alen Protic, Antonio Piralla, Qiang Pan-Hammarstroem, Luka Cicin-Sain, Michael Hust
Summary: This study analyzed the binding ability of the Omicron variant to the human receptor and its neutralization efficacy. The results showed weaker binding and complete loss of neutralization against Omicron compared to previous variants. However, booster vaccination increased the antibody levels and partially restored neutralization against Omicron SARS-CoV-2.
Article
Multidisciplinary Sciences
Fanglei Zuo, Hassan Abolhassani, Likun Du, Antonio Piralla, Federico Bertoglio, Leire de Campos-Mata, Hui Wan, Maren Schubert, Irene Cassaniti, Yating Wang, Jose Camilla Sammartino, Rui Sun, Stelios Vlachiotis, Federica Bergami, Makiko Kumagai-Braesch, Juni Andrell, Zhaoxia Zhang, Yintong Xue, Esther Veronika Wenzel, Luigi Calzolai, Luca Varani, Nima Rezaei, Zahra Chavoshzadeh, Fausto Baldanti, Michael Hust, Lennart Hammarstrom, Harold Marcotte, Qiang Pan-Hammarstrom
Summary: This study demonstrates that an mRNA vaccine booster in individuals vaccinated with inactivated vaccines can significantly increase the humoral and cellular immune responses against the virus, including the Omicron variant.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Immunology
Fanglei Zuo, Guorong Liu, Junhua Jin
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fanglei Zuo, Tanvi Somiah, Hanna G. G. Gebremariam, Ann-Beth Jonsson
Summary: This study investigated the effect of Lactobacillus culture supernatants on the expression of transcriptional regulators in H. pylori. The results showed that some lactobacilli can downregulate the expression of transcriptional regulators involved in motility, acid tolerance, and LL-37 sensitivity of H. pylori.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Yoshikazu Yuki, Fanglei Zuo, Shiho Kurokawa, Yohei Uchida, Shintaro Sato, Naomi Sakon, Lennart Hammarstroem, Hiroshi Kiyono, Harold Marcotte
Summary: Passive administration of neutralizing antibodies against intestinal pathogens like norovirus is a promising strategy for gastrointestinal infection control. However, the challenge lies in ensuring the stability and sufficient amounts of orally administered antibodies in the harsh gastrointestinal environment. In this study, researchers expressed a single-domain neutralizing antibody against norovirus on the cell surface of Lactobacillus, a natural gut commensal. The engineered lactobacilli successfully inhibited norovirus replication in vitro and showed neutralizing activity in the intestines of mice for at least 10 days after oral administration. These cell-wall-anchored antibody-displaying lactobacilli serve as attractive oral delivery vectors for passive immunization against norovirus infection.