Article
Materials Science, Biomaterials
Lifeng Chen, Shan Mou, Jinfei Hou, Huimin Fang, Yuyang Zeng, Jiaming Sun, Zhenxing Wang
Summary: The surface modification of medical metal biomaterial titanium implants using bioactive molecules such as stem cell-derived extracellular vesicles showed enhanced osteoblast compatibility and osteoinductive activity. This method provides a clinically translatable strategy to promote the bioactivity of titanium implants.
REGENERATIVE BIOMATERIALS
(2021)
Article
Cell & Tissue Engineering
Xiaowen Hu, Jiaji Pan, Yongfang Li, Yixu Jiang, Haoran Zheng, Rubing Shi, Qi Zhang, Chang Liu, Hengli Tian, Zhijun Zhang, Yaohui Tang, Guo-Yuan Yang, Yongting Wang
Summary: This study found that ADSC-EVs have therapeutic potential in ischemic brain injury by regulating microglial polarization. MiRNAs in ADSC-EVs partially contribute to their function by targeting PTEN and STAT1 to regulate microglial polarization.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Review
Immunology
Qingyi Jia, Hanxing Zhao, Yixi Wang, Ying Cen, Zhenyu Zhang
Summary: Wound healing is a complex process that involves multiple phases, including inflammation, proliferation, and remodeling. Abnormal inflammation can impede wound healing under certain pathological conditions. Adipose-derived stem cells (ADSCs) and their secreted extracellular vesicles (ADSC-EVs) have shown potential in promoting wound healing by inhibiting abnormal inflammation responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shih-Heng Chen, Zhi-Yu Chen, Ya-Hsuan Lin, Shih-Hsien Chen, Pang-Yun Chou, Huang-Kai Kao, Feng-Huei Lin
Summary: This study demonstrated that extracellular vesicles (EVs) derived from adipose-derived stem cells (ADSCs) significantly enhanced tendon regeneration by promoting proliferation and migration of tenocytes. Treatment with ADSC-EVs also improved the mechanical strength of repaired tendons, suggesting a potentially highly potent therapeutic strategy for tendon injuries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Mariachiara Zuccarini, Patricia Giuliani, Valentina Di Liberto, Monica Frinchi, Francesco Caciagli, Vanni Caruso, Renata Ciccarelli, Giuseppa Mudo, Patrizia Di Iorio
Summary: In the past decade, several compounds have been identified for treating obesity, but concerns about their efficacy and safety remain due to the complexity of the disease. Therefore, it is crucial to discover new factors involved in obesity induction and progression. Adipose stromal/stem cells release extracellular vesicles (EVs), which play an important role in regulating metabolic processes. However, there are advantages and disadvantages to using EVs as potential next-generation anti-obesity agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yan Yan, Tingting Gu, Stine Duelund Kaas Christensen, Junyi Su, Thomas Ravn Lassen, Marie Vognstoft Hjortbak, IJu Lo, Susanne Trillingsgaard Veno, Andrea Erzsebet Toth, Ping Song, Morten Schallburg Nielsen, Hans Erik Botker, Blagoy Blagoev, Kim Ryun Drasbek, Jorgen Kjems
Summary: Remote ischemic conditioning can attenuate ischemic-reperfusion injury by regulating the release of extracellular vesicles (EVs) from cells to provide protective function to damaged organs. This study showed that cyclic hypoxia-reoxygenation (HR) induced changes in the miRNA and protein profile of EVs released by C2C12 cells, enhancing the protective signal to recipient cells in vitro. Additionally, HR EV treatment restored cell viability, reduced inflammation, and improved tube formation in in vitro assays.
Article
Agriculture, Dairy & Animal Science
Byong Seung Cho, Sung-Bae Kim, Sokho Kim, Beomseok Rhee, Jungho Yoon, Jae Won Lee
Summary: In this study, the effects of canine ASC-exosomes on canine AD were investigated using a Biostir-induced AD mouse model. The study found that cASC-EVs improved AD-like dermatitis and decreased serum IgE, ear thickness, and inflammatory cytokines such as IL-4 and IFN-γ in a dose-dependent manner. In vivo toxicity studies showed no systemic toxicity. miRNA analysis showed the presence of anti-inflammatory miRNAs in cASC-EVs, suggesting a promising cell-free therapy for treating canine AD.
Article
Chemistry, Multidisciplinary
Xing Pei, Jiyoung Shin, Heejung Kim, Nana Wang, Chaewon Seo, Miyun Yoon, Xiongwen Chen, Jianqing Gao, Victor C. Yang, Huining He, Seungjin Lee
Summary: Stem cell-based therapeutic approach for critical limb ischemia aims at revascularization and tissue regeneration, with cardiac-derived stem cells showing potential as a novel cell source due to their superior endothelial differentiation ability. Genetically engineering CSCs with constitutively active HIF-1 alpha can enhance angiogenic function and promote new vessel formation, providing a promising treatment modality for CLI.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Cell & Tissue Engineering
Weihui Li, Wei Wang, Xin He, Zheng Liao, Aili Aierken, Jinlian Hua, Yan Wang, Dezhang Lu, Shiqiang Zhang
Summary: The therapeutic efficacy of allogeneic adipose mesenchymal stem cell-derived extracellular vesicles (ADMSCEVs) on cat models of PR-AKI was investigated in this study. The results showed that ADMSCEVs efficiently restored the levels of creatinine, urea nitrogen, and plasma phosphorus. Blood routine analysis indicated faster recovery of leukocytes in PR-AKI cats treated with ADMSCEVs. Additionally, plasma metabolome profile analysis identified six metabolites highly correlated with the dynamic process of PR-AKI.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Hang Zhao, Xiyao Chen, Guangyu Hu, Congye Li, Lanyan Guo, Ling Zhang, Fangfang Sun, Yunlong Xia, Wenjun Yan, Ze Cui, Yongzhen Guo, Xiong Guo, Chong Huang, Miaomiao Fan, Shan Wang, Fuyang Zhang, Ling Tao
Summary: This study found that BAT participates in regulating cardiomyocyte survival and exercise cardioprotection through the secretion of sEVs. The study also identified BAT miRNAs as essential components for mediating cardioprotection and revealed their mechanism of action involving suppression of the MAPK pathway.
CIRCULATION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Jiahe Guo, Xiaofan Yang, Jing Chen, Cheng Wang, Yue Sun, Chengqi Yan, Sen Ren, Hewei Xiong, Kaituo Xiang, Maojie Zhang, Chengcheng Li, Guoyong Jiang, Xuejiao Xiang, Gui Wan, Tao Jiang, Yu Kang, Xiang Xu, Zhenbing Chen, Wenqing Li
Summary: This study found that adipose-derived mesenchymal stem cell-derived exosomes (ADSC-Exos) have a protective effect on diabetic lower limb ischemic injury through the action of miR-125b-5p. They can promote cell proliferation, migration, and angiogenesis, thus accelerating tissue repair and vascular regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Endocrinology & Metabolism
Hongyan Deng, Yong Chen
Summary: Diabetic foot ulcer is a severe complication of type 2 diabetes mellitus, which is difficult to treat. Adipose tissue dysfunction and insulin resistance play a major role, but adipose tissue has been found to secrete bio-active factors. Adipose tissue-derived stem cells and their secreted extracellular vesicles have significant effects on wound repair.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell & Tissue Engineering
Yanan Tang, Jiayan Li, Weiyi Wang, Bingyi Chen, Jinxing Chen, Zekun Shen, Jiaxuan Hou, Yifan Mei, Shuang Liu, Liwei Zhang, Zongjin Li, Shaoying Lu
Summary: The study demonstrated that platelet extracellular vesicles (PEVs) can enhance the proangiogenic potential of adipose-derived mesenchymal stem cells (ADSCs) by promoting cell proliferation, migration, anti-apoptosis ability, and paracrine secretion.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Denise Traxler, Varius Dannenberg, Katrin Zlabinger, Alfred Gugerell, Julia Mester-Tonczar, Dominika Lukovic, Andreas Spannbauer, Ena Hasimbegovic, Jens Kastrup, Mariann Gyoengyoesi
Summary: This study found that the administration of ADSCs via intramyocardial injection had no significant effect on cardiac function in patients with ischemic heart disease, and did not regulate the concentration of small EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Damiano Patrono, Davide Cussa, Veronica Sciannameo, Elena Montanari, Rebecca Panconesi, Paola Berchialla, Mirella Lepore, Alessandro Gambella, Giorgia Rizza, Giorgia Catalano, Stefano Mirabella, Francesco Tandoi, Francesco Lupo, Roberto Balagna, Mauro Salizzoni, Renato Romagnoli
Summary: Dual hypothermic oxygenated machine perfusion (D-HOPE) improves preservation and outcomes in liver transplantation, especially when utilizing donors after neurological determination of death (DBD). D-HOPE reduces early graft failure, complications, and improves survival for both patients and grafts, including elderly donors.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Immunology
Francesco Tandoi, Licia Peruzzi, Pietro Lonardi, Davide Cussa, Silvia Catalano, Aldo Verri, Maurizio Merlo, Omidreza Sedigh, Simona Gerocarni Nappo, Claudia Melloni, Damiano Patrono, Bruno Gianoglio, Renato Romagnoli
Summary: This study reports on two pediatric patients who were unable to undergo kidney transplantation due to thrombosis or aberrant anatomy in the iliac-caval system. The patients were successfully managed by using splenic vessels as a source of arterial inflow or venous drainage during the transplantation.
Article
Gastroenterology & Hepatology
Damiano Patrono, Riccardo De Carlis, Alessandro Gambella, Francesca Farnesi, Alice Podesta, Andrea Lauterio, Francesco Tandoi, Luciano De Carlis, Renato Romagnoli
Summary: The viability of liver grafts with significant MaS (>= 30%) has not been specifically assessed in end-ischemic NMP. Our study found that NMP allowed successful transplantation of 57% of livers with moderate-to-severe MaS. Prolonged observation and stable lactate clearance are important for the use of steatotic livers.
LIVER TRANSPLANTATION
(2023)
Review
Biochemistry & Molecular Biology
Concetta Panebianco, Annacandida Villani, Adele Potenza, Enrica Favaro, Concetta Finocchiaro, Francesco Perri, Valerio Pazienza
Summary: Cancer cachexia is a complex syndrome characterized by weight loss, anorexia, inflammation, and metabolic alterations, which negatively impact cancer patients. Studies have shown a link between gut microbiota and cancer cachexia, suggesting a role for dysbiosis in its pathogenesis. Different manipulation tools of gut microbiota have shown promising improvements in cachexia outcomes, paving the way for future research and clinical interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Margherita Saracco, Francesco Tandoi, Francesca Maletta, Roberto Balagna, Renato Romagnoli, Silvia Martini
Summary: This study aimed to evaluate the safety and efficacy of DAA therapy started as soon as possible after liver transplant. Among 490 liver transplants, 49 (10%) patients were still HCV viraemic at operation, but almost all patients achieved HCV eradication after DAA treatment.
JOURNAL OF VIRAL HEPATITIS
(2023)
Article
Biochemistry & Molecular Biology
Silvia Corcione, Ilaria De Benedetto, Nour Shbaklo, Fabio Ranzani, Simone Mornese Pinna, Anna Castiglione, Silvia Scabini, Gabriele Bianco, Rossana Cavallo, Stefano Mirabella, Renato Romagnoli, Francesco Giuseppe De Rosa
Summary: This study retrospectively analyzed the characteristics and impact of treatment on mortality in patients with KPC-Kp bloodstream infections. The results showed that source control and accurate timing of treatment were more important than the choice between monotherapy or combination therapy, as well as the use of ceftazidime/avibactam.
Article
Nutrition & Dietetics
Chiara D'Eusebio, Fabio Dario Merlo, Marta Ossola, Fabio Bioletto, Mirko Ippolito, Monica Locatelli, Antonella De Francesco, Marta Anro, Renato Romagnoli, Paolo Strignano, Simona Bo, Umberto Aimasso
Summary: This retrospective cohort study used competing risk analysis to evaluate the rates and predictors of mortality and weaning in patients with chronic intestinal failure. The study found that surgical procedures strongly influenced the risk of mortality and weaning in these patients.
Article
Immunology
Matteo Rinaldi, Cecilia Bonazzetti, Mena Gallo, Giuseppe Ferraro, Maristela Freire, Debora Raquel Benedita Terrabuio, Francesco Tandoi, Renato Romagnoli, Francesco Giuseppe De Rosa, Alessandra Mularoni, Alberto Ferrarese, Patrizia Burra, Marcia Halpern, Elizabeth Balbi, Jacques Simkins, Lilian Abbo, Ignacio Morras, Mireia Cantero, Laura Alagna, Alessandra Bandera, Wanessa Trinidade Clemente, Maricela Valerio, Ainhoa Fernandez, Patricia Munoz, Liran Statlender, Dafna Yahav, Luis Fernando Aranha Camargo, Evelyne Santana Girao, Paolo Grossi, Pierluigi Viale, Stefania Curti, Maddalena Giannella
Summary: This study highlights the importance of managing infections in solid organ transplant recipients. Both the INCREMENT-SOT-CPE score and the SOFA score were identified as strong predictors of all-cause 30-day mortality in these patients.
TRANSPLANT INFECTIOUS DISEASE
(2023)
Review
Medicine, General & Internal
Damiano Patrono, Fabio Colli, Matteo Colangelo, Nicola De Stefano, Ana Lavinia Apostu, Elena Mazza, Silvia Catalano, Giorgia Rizza, Stefano Mirabella, Renato Romagnoli
Summary: Perihilar cholangiocarcinomas (pCCA) are aggressive tumors originating from the bile ducts. Liver transplantation (LT) after neoadjuvant chemoradiation has shown promising results, with 5-year survival rates consistently >50%. Machine perfusion (MP) technology could be utilized in LT for pCCA to improve liver preservation and expand the donor pool. This review highlights current surgical strategies for pCCA treatment and discusses the potential benefits of MP in this setting.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Health Care Sciences & Services
Federica Rigo, Nicola De Stefano, Damiano Patrono, Victor De Donato, Ludovico Campi, Diana Turturica, Teresa Doria, Veronica Sciannameo, Paola Berchialla, Francesco Tandoi, Renato Romagnoli
Summary: The study aimed to investigate the impact of dual-hypothermic oxygenated machine perfusion (D-HOPE) on hepatocellular carcinoma (HCC) recurrence after liver transplantation (LT). The results showed that D-HOPE did not reduce HCC recurrence but allowed the use of extended criteria donor livers, with comparable postoperative outcomes to static cold storage (SCS).
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Medicine, General & Internal
Damiano Patrono, Nicola De Stefano, Elena Vissio, Ana Lavinia Apostu, Nicoletta Petronio, Giovanni Vitelli, Giorgia Catalano, Giorgia Rizza, Silvia Catalano, Fabio Colli, Luigi Chiusa, Renato Romagnoli
Summary: Liver allograft steatosis poses a significant risk to transplant outcomes and survival. With the increasing prevalence of obesity and fatty liver disease, the use of steatotic liver grafts in transplantation has become more common. This review examines the mechanisms behind fatty liver susceptibility to ischemia-reperfusion injury and explores strategies, including donor interventions, preservation solutions, and machine perfusion techniques, to enhance the utilization of steatotic livers for transplantation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Nicola De Stefano, Alberto Calleri, Angelo Corso Faini, Victor Navarro-Tableros, Silvia Martini, Silvia Deaglio, Damiano Patrono, Renato Romagnoli
Summary: Extracellular vesicles (EVs) are small, membrane-bound vesicles that contain various bioactive molecules and play important roles in intercellular communication. In the field of liver disease research, EVs are emerging as a promising topic with potential applications in liver transplantation. This review provides an overview of the current understanding of the role of EVs in liver transplantation, including their potential as biomarkers and therapeutic agents, as well as their involvement in graft rejection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Eishan Ashwat, Christof Kaltenmeier, Hao Liu, Dheera Reddy, Ann Thompson, Stalin Dharmayan, Subhashini Ayloo, Silvio Nadalin, Olga Ciccarelli, Qingyong Xu, Rene Adam, Vincent Karam, Krzystof Zieniewicz, Darius Mirza, Michael Heneghan, Renato Romagnoli, Andreas Paul, Daniel Cherqui, Johann Pratschke, Karim Boudjema, Peter Schemmer, Fernando San Juan Rodriguez, Peter Lodge, Paolo de Simone, Phillippe Bachellier, Jiri Fronek, Constantino Fondevila, Michele Molinari
Summary: The Liver Transplant Risk Score (LTRS) is a clinical tool used to predict post liver transplant outcomes. This study validated the performance of the LTRS in a cohort of European patients.
BRITISH JOURNAL OF SURGERY
(2023)
Meeting Abstract
Surgery
I. M. Bruggenwirth, M. Mueller, V. A. Lantinga, S. Camagni, R. De Carlis, L. De Carlis, M. Colledan, D. Dondossola, M. Drefs, J. Eden, D. Ghinolfi, D. Koliogiannis, G. Lurje, T. Manzia, D. Monbaliu, P. Muisean, D. Patrono, J. Pratschke, R. Romagnoli, M. Rayar, F. Roma, A. Schlegel, P. Dutkowski, R. J. Porte, V. E. de Meijer
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Review
Medicine, General & Internal
Nicola De Stefano, Alberto Calleri, Victor Navarro-Tableros, Federica Rigo, Damiano Patrono, Renato Romagnoli
Summary: Transplantation is the preferred treatment for end-stage liver diseases, but there is a shortage of donor organs. Machine perfusion technology and mesenchymal stem cell therapy can improve outcomes after liver transplantation and provide new methods for external treatment.
MEDICINA-LITHUANIA
(2022)
