Article
Multidisciplinary Sciences
Arnab Das, Sudarshana Basu, Diptankar Bandyopadhyay, Kamalika Mukherjee, Debduti Datta, Sreemoyee Chakraborty, Sayantan Jana, Moumita Adak, Sarpita Bose, Saikat Chakrabarti, Snehasikta Swarnakar, Partha Chakrabarti, Suvendra N. Bhattacharyya
Summary: miR-122 in the mammalian liver regulates metabolic homeostasis and plays a role in controlling tissue inflammation through intercellular transfer. MMP2 is essential for the transfer of extracellular vesicles and their miRNA content between hepatic and non-hepatic cells.
Article
Oncology
Hossein Sendi, Mostafa Yazdimamaghani, Mengying Hu, Nikhila Sultanpuram, Jie Wang, Amber S. Moody, Ellie McCabe, Jiajie Zhang, Amanda Graboski, Liantao Li, Juan D. Rojas, Paul A. Dayton, Leaf Huang, Andrew Z. Wang
Summary: Efficient delivery of miR-122 to hepatocytes using a nanomedicine-based formulation has the potential to prevent and treat colorectal cancer liver metastasis. Mechanistically, it downregulates key genes involved in metastatic and cancer inflammation pathways, increases CD8(+)/CD4(+) T-cell ratio, and decreases immunosuppressive cell infiltration, making the liver more conducive to antitumor immune response.
Article
Cardiac & Cardiovascular Systems
Miruna Nemecz, Diana Simona Stefan, Ioana Karla Comarita, Alina Constantin, Gabriela Tanko, Cristian Guja, Adriana Georgescu
Summary: In this study, miR-218, miR-132, and miR-143 were identified as significantly up-regulated miRNAs in both circulating MVs and plasma from diabetic patients with dyslipidemia. These miRNAs showed significant correlations with important plasma markers representative of this pathology.
CARDIOVASCULAR DIABETOLOGY
(2023)
Article
Orthopedics
K. M. Scott, D. J. Cohen, M. Hays, D. W. Nielson, M. W. Grinstaff, T. B. Lawson, B. D. Snyder, B. D. Boyan, Z. Schwartz
Summary: Both miR-122 and miR-451 were upregulated in OA in a rat bilateral anterior cruciate ligament transection (ACLT) model. miR-122 prevented the effects of IL-1 beta on rArCs, while miR-451 exacerbated them.
OSTEOARTHRITIS AND CARTILAGE
(2021)
Article
Medical Laboratory Technology
Yujia Fang, Dong Yan, Lixin Wang, Jie Zhang, Qingfang He
Summary: This study analyzed the differential expression of miRNAs between HCC patients and controls and found that miR-16 and miR-122 were significantly upregulated in early-stage HCC patients. Combining these miRNAs with AFP improved the diagnostic efficacy for HCC. Furthermore, miR-16 and miR-122 were found to regulate a number of potential target genes involved in cancer pathways.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Shohei Kaneko, Katsunori Yanai, Hiroki Ishii, Akinori Aomatsu, Keiji Hirai, Susumu Ookawara, Kenichi Ishibashi, Yoshiyuki Morishita
Summary: The role of exogenous miRNAs in renal fibrosis was investigated using a mouse model. miR-122-5p was found to be downregulated in the kidneys of mice with renal fibrosis. Overexpression of miR-122-5p promoted fibrosis, while inhibition of miR-122-5p suppressed fibrosis. miR-122-5p regulated the expression of fibrosis-related mRNAs, including pro-fibrotic TGFBR2 and anti-fibrotic FOXO3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yosuke Inomata, Jae-Won Oh, Kohei Taniguchi, Nobuhiko Sugito, Nao Kawaguchi, Fumitoshi Hirokawa, Sang-Woong Lee, Yukihiro Akao, Shinji Takai, Kwang-Pyo Kim, Kazuhisa Uchiyama
Summary: The pathophysiology of non-alcoholic steatohepatitis (NASH) was investigated from the perspective of glycolysis and the Warburg effect, with a focus on microRNA regulation in liver-specific macrophages. The activation of glycolysis in Kupffer cells during NASH was partially induced by the upregulation of pyruvate kinase muscle 2 (PKM2) via microRNA122-5p (miR-122-5p) suppression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Mamata Panigrahi, Michael A. A. Palmer, Joyce A. A. Wilson
Summary: The 5 ' untranslated region (UTR) of the hepatitis C virus (HCV) genome regulates virus replication and translation. Binding of miR-122 to the 5 ' UTR regulates viral replication and translation, but its precise mechanism is still unresolved. Our study shows that miR-122 binding stimulates viral translation and that enhancing translation and stabilizing the viral genome can rescue miR-122-independent HCV replication. This suggests that miR-122 plays a primary role in promoting HCV translation and genome stabilization.
JOURNAL OF VIROLOGY
(2023)
Article
Infectious Diseases
Yung-Che Chen, Chang -Chun Hsiao, Chao-Chien Wu, Tung-Ying Chao, Sum-Yee Leung, Yu -Ping Chang, Chia-Cheng Tseng, Chiu-Ping Lee, Po-Yuan Hsu, Ting-Ya Wang, Po-Wen Wang, Ting-Wen Chen, Meng-Chih Lin
Summary: This study explores a new approach for treating active tuberculosis (TB) by screening and validating microRNAs with immune-regulatory functions. The research identified differentially expressed microRNAs associated with TB and revealed their roles in regulating autophagy, apoptosis, and macrophage phagocytosis. The findings provide evidence for microRNA-based host-directed immunotherapy and offer new therapeutic targets for treatment-refractory TB.
JOURNAL OF INFECTION
(2022)
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Serap Ozer Yaman, Asim Orem, Fulya Balaban Yucesan, Birgul Vanizor Kural, Cihan Orem
Summary: The study identified alterations in the expression levels of miR-122 and miR-33a, as well as the potential value of the miR-122/30c ratio in postprandial lipemia in subjects with normal and high postprandial response.
Article
Biology
Zhaowei Wang, Xiaoling Xia, Jiaqi Li, Tatsushi Igaki
Summary: Research has discovered that miR-306 and miR-79, which act as tumor suppressor miRNAs, can eliminate activated tumors by overactivating JNK signaling via a noncanonical pathway, providing a potential new strategy for cancer treatment.
Article
Agronomy
Zhong-Jian Shen, Fang Zhu, Yan-Jun Liu, Zhen Li, Timothy W. Moural, Xiao-Ming Liu, Xiaoxia Liu
Summary: The study investigates the regulatory functions of dopamine (DA) and its associated non-coding RNA in the metamorphosis of the cotton bollworm, Helicoverpa armigera. It is found that DA deficiency inhibits larval-pupal metamorphosis in H. armigera. The study suggests that DA pathway genes and their microRNAs could be used as a novel target for H. armigera management.
PEST MANAGEMENT SCIENCE
(2022)
Article
Cell Biology
Fabiola Olivieri, Francesco Prattichizzo, Angelica Giuliani, Giulia Matacchione, Maria Rita Rippo, Jacopo Sabbatinelli, Massimiliano Bonafe
Summary: Inflammaging is a key concept in understanding how aging contributes to the development of age-related diseases, with a focus on the role of miRNAs in regulating inflammatory pathways. The assessment of individual genetic make-up alone is insufficient to depict the trajectories of age-related inflammation, and studies have shown that circulating inflammamiRs could be used to measure the degree of inflammaging.
AGEING RESEARCH REVIEWS
(2021)
Article
Oncology
Ning Hu, Yanhua Tian, Yanmei Song, Leilei Zang
Summary: miR-122-5p plays a crucial role in regulating the occurrence and development of papillary thyroid carcinoma (PTC) by inhibiting the tumor-promoting gene DUSP4, thereby suppressing the proliferation and metastasis of PTC cells, revealing its potential diagnostic and therapeutic value in PTC.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Developmental Biology
Annika Balraj, Cheryl Clarkson-Paredes, Ahdeah Pajoohesh-Ganji, Matthew W. Kay, David Mendelowitz, Robert H. Miller
Summary: This study examines the relationship between axon conduction and myelination in the maturing optic nerve. The results show that axon diameter, ion channel subtype, and myelin thickness are key regulators of nerve function. These findings suggest an extended period of maturation in the normal optic nerve that facilitates the development of visual signaling patterns.
DEVELOPMENTAL NEUROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Shinnosuke Tsuji, Calvin J. Stephens, Giulia Bortolussi, Feijie Zhang, Gabriele Baj, Hagoon Jang, Gustavo de Alencastro, Andres F. Muro, Katja Pekrun, Mark A. Kay
Summary: The use of ribonucleotide reductase inhibitors can substantially enhance the efficiency of adeno-associated virus-mediated homologous recombination-based gene therapy. These findings suggest that clinically approved inhibitors, such as fludarabine, can be used to potentiate gene editing therapies.
NATURE BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Chang Liu, Pengpeng Yan, Xiaoyu Xu, Wenhui Zhou, Dhayakumar Rajan Prakash, Shuqi Wang, Junnian Zhou, Rending Wang, Hongfeng Huang, Jianghua Chen, Hongbo Zhang, Jia Shen
Summary: By utilizing a site-specific nanovehicle, this study presents a novel approach for treating ABMR. The nanovehicle is capable of targeting the affected allograft kidney endothelium under ABMR and releasing an anti-inflammatory drug upon laser irradiation to promote endothelial recovery. This strategy holds potential for future clinical applications.
Article
Physiology
Joyce Lin, Anand Abraham, Sharon A. George, Amara Greer-Short, Grace A. Blair, Angel Moreno, Bridget R. Alber, Matthew W. Kay, Steven Poelzing
Summary: Many cardiac pathologies are associated with reduced gap junction coupling. The relationship between the phenotype and functional expression of the connexin gap junction family of proteins is controversial. Low gap junction coupling can be explained by ephaptic coupling theory and can also increase intracellular charge accumulation, resulting in faster transmembrane potential rate of change during depolarization. Studies suggest that conduction reserve is more dependent on ephaptic coupling than charge accumulation during gap junction uncoupling.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Genetics & Heredity
Samuel N. Smukowski, Heather Maioli, Caitlin S. Latimer, Thomas D. Bird, Suman Jayadev, Paul N. Valdmanis
Summary: Amyotrophic lateral sclerosis (ALS) is a prominent motor neuron disease in humans. Several causative genes have been identified, and additional variants are being studied for their association with ALS. Understanding ALS genetics can lead to the development of effective treatments.
NEUROLOGY-GENETICS
(2022)
Article
Cardiac & Cardiovascular Systems
Sarah Kuzmiak-Glancy, Brian Glancy, Matthew W. Kay
Summary: Myocardial ischemia has long-lasting negative impacts on cardiomyocyte mitochondrial ATP production. The location of damage and specific factors controlling mitochondrial function alteration following ischemia remain unclear. In this study, it was found that ischemia lowered maximal mitochondrial respiration rates, diminished the responsiveness of respiration to driving forces, and decreased the activities of every component of the oxidative phosphorylation pathway. Ischemia also led to higher ROS production. These findings highlight the importance of targeting the entire mitochondrial energy conversion cascade to improve mitochondrial function following ischemia.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Clinical Neurology
Meredith M. Course, Kathryn Gudsnuk, C. Dirk Keene, Thomas D. Bird, Suman Jayadev, Paul N. Valdmanis
Summary: Course et al. performed targeted long-read isoform-sequencing of PSEN1 and PSEN2 in individuals with sporadic Alzheimer's disease, familial Alzheimer's disease with PSEN1 and PSEN2 pathogenic variants, and controls. The study revealed unique aberrant splicing of PSEN2 in sporadic Alzheimer's disease, expanding our understanding of PSEN1 and PSEN2 variants in Alzheimer's disease and suggesting novel mechanisms of Alzheimer's disease pathogenesis.
Article
Medicine, Research & Experimental
Francesco Puzzo, Chuanling Zhang, Bethany Powell Gray, Feijie Zhang, Bruce A. Sullenger, Mark A. Kay
Summary: This study developed a new chemically modified AAV vector, N & epsilon;-AAVs, which allows post-production engineering through click chemistry. The functionalized N & epsilon;-AAVs showed increased transduction efficiency and demonstrated selective uptake in vivo, making them versatile gene therapy vectors for specific cell targeting.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Letter
Biochemical Research Methods
Andrew D. Holmes, Patricia P. Chan, Qi Chen, Pavel Ivanov, Laurence Drouard, Norbert Polacek, Mark A. Kay, Todd M. Lowe
Article
Multidisciplinary Sciences
Adriana Gonzalez-Sandoval, Katja Pekrun, Shinnosuke Tsuji, Feijie Zhang, King L. Hung, Howard Y. Chang, Mark A. Kay
Summary: The effectiveness of rAAV vectors varies between species, and a single amino acid change in the vector can alter its epigenomic state and transgene expression levels between species.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Saebyeol Lee, Jungeun Kim, Paul N. N. Valdmanis, Hak Kyun Kim
Summary: Transfer RNAs (tRNAs) play a crucial role in mRNA translation by delivering amino acids to polypeptide chains. tRNAs can be cleaved by ribonucleases, resulting in tRNA-derived small RNAs (tsRNAs) with various regulatory functions. tsRNAs have been shown to have both oncogenic and tumor suppressor functions, and their abnormal expression and modifications are associated with various diseases. In this review, the biogenesis, gene regulation mechanisms, and potential therapeutic roles of tsRNAs in cancer are discussed.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
Katja Pekrun, Calvin J. Stephens, Feijie Zhang, Lauren Kelly, Natacha Le Moan, Alice F. Tarantal, Grant E. Blouse, Mark A. Kay
Meeting Abstract
Biotechnology & Applied Microbiology
Francesco Puzzo, Chuanling Zhang, Feijie Zhang, Mark A. Kay
Meeting Abstract
Biotechnology & Applied Microbiology
Francesco Puzzo, Feijie Zhang, Katja Pekrun, Mark A. Kay
Article
Engineering, Biomedical
Guohua Wu, Jianguo Wu, Zihan Li, Shengyu Shi, Di Wu, Xuanbo Wang, Han Xu, Hui Liu, Yixiao Huang, Rending Wang, Jia Shen, Zhihong Dong, Shuqi Wang
Summary: The development of a digital organ-on-a-chip platform provides a high-parallelism and low-variability analytical tool for toxicity assessment and the exploration of new anticancer modalities, accelerating the joint endeavor to combat cancer.
BIO-DESIGN AND MANUFACTURING
(2022)
