Article
Entomology
Asifa Hameed, Jonah M. Ulmer, Istvan Miko, Cristina Rosa, Edwin G. Rajotte
Summary: This study describes the structure and movement mechanism of the female reproductive system of soybean thrips, as well as their egg-laying behavior. It provides important insights into the virus transmission mechanism of soybean thrips and the development of management strategies.
Article
Entomology
Asifa Hameed, Cristina Rosa, Edwin G. Rajotte
Summary: Soybean vein necrosis, caused by soybean vein necrosis virus (SVNV), is an important viral disease that affects soybeans. The virus infection increases survival, longevity, reproduction rate, and life expectancy of the vector thrips, while decreasing population doubling time. This study provides evidence that virus infection enhances the virus and vector populations in soybeans.
Article
Biochemistry & Molecular Biology
Hao-Wen Cheng, Wei-Ting Tsai, Yi-Ying Hsieh, Kuan-Chun Chen, Shyi-Dong Yeh
Summary: This study demonstrated that the np sequence and TNP MAb can be effectively applied for tagging and detecting proteins, and can be coupled with the nss-tag to form a novel epitope-tagging system for investigating protein-protein interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Zhe Wang, Xuhong Song, Rex Frimpong Anane, Lu Chen, Zeli Chen, Like Gao, Shangyun Li, Guosong Wen, Mingfu Zhao
Summary: This study reports the infection of Orthotospovirus in medicinal plants. Using ELISA and RT-PCR analysis, the presence of different viruses in multiple medicinal plants was identified, and phylogenetic tree analysis confirmed the consistency of sequences.
JOURNAL OF PHYTOPATHOLOGY
(2022)
Article
Plant Sciences
Gabriel Madoglio Favara, Felipe Franco de Oliveira, Camila Geovana Ferro, Heron Delgado Kraide, Eike Yudi Nishimura Carmo, Vinicius Henrique Bello, Marcos Roberto Ribeiro-Junior, Renate Krause-Sakate, Elliot Watanabe Kitajima, Jorge Alberto Marques Rezende
Summary: Plumeria pudica, also known as bridal bouquet, was found to have characteristic symptoms of groundnut ringspot virus (GRSV) infection in different areas of Brazil. The virus was poorly transmitted mechanically to P. pudica and predominantly present in symptomatic leaf blades. Symptomatic branches of infected plants propagate vegetatively and result in plants expressing GRSV symptoms, while asymptomatic branches predominantly generate plants without GRSV symptoms. The resistance of P. pudica to GRSV infection suggests that this orthotospovirus does not pose a threat to this ornamental plant.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Virology
Hongwei Wang, Xiujuan Wu, Xiande Huang, Shujun Wei, Zhijun Lu, Jian Ye
Summary: This study provides evidence for the seed-borne transmission of Tomato spotted wilt orthotospovirus (TSWV) in pepper plants. The study shows that TSWV can be transmitted through seeds, with the endosperm being the main virus-containing seed organ. These findings are significant for researchers, certification agencies, the seed industry, and policy makers when evaluating the importance of TSWV in vegetable production worldwide.
Article
Microbiology
Afsha Tabassum, S. V. Ramesh, Ying Zhai, Romana Iftikhar, Cristian Olaya, Hanu R. Pappu
Summary: The research identified two major genotypes of Iris yellow spot virus, IYSVNL and IYSVBR, with different genetic diversity and evolutionary patterns. IYSVNL and IYSVBR genotypes are under purifying selection and population expansion, while IYSVother genotype shows decreasing population size and appears to be under balancing selection. Substitution rates suggest that IYSV continues to evolve using population expansion strategies, similar to other plant RNA viruses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Horticulture
Siddharood Maragal, Eguru Sreenivasa Rao, D. C. Lakshmana Reddy, D. K. Samuel
Summary: The study explored efforts to integrate WBNV resistance QTLs through QTL-meta-analysis and JICIM, identifying potential candidate QTL regions and genes for WBNV resistance in watermelon. These results may pave the way for functional validation of putative candidates and the development of suitable markers for marker assisted selection of WBNV resistance in watermelon.
SCIENTIA HORTICULTURAE
(2021)
Article
Plant Sciences
Min Gui, Huaran Hu, Zhiqiang Jia, Xue Gao, Hongzheng Tao, Yongzhong Li, Yating Liu
Summary: Viruses can infect plants more efficiently by forming complexes with another virus. This study found that the tomato spotted wilt orthotospovirus (TSWV) and Hippeastrum chlorotic ringspot orthotospovirus (HCRV) can infect tobacco plants in cooperation with each other, resulting in a higher infection rate. Through transcriptome analysis, it was discovered that co-infection alters the plant's antiviral response and suppresses plant basal resistance by regulating miRNAs. These findings contribute to the development of new strategies for controlling crop diseases caused by this virus complex.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Doris M. Lagos-Kutz, Michelle L. Pawlowski, Jaeyeong Han, Steven J. Clough, Glen L. Hartman
Summary: Soybean thrips is a widespread pest that affects the growth of soybean and spreads Soybean Vein Necrosis Virus. The severity of infection and the resistance of soybean genotypes are correlated.
Article
Agronomy
Faten Mansouri, Frantisek Krahulec, Martin Duchoslav, Pavel Rysanek
Summary: Studies have shown that plant viruses are more commonly found in cultivated Allium species compared to those in the wild. The presence of 15 different viruses was detected in 65 Allium species in the Czech Republic, with infection rates varying based on habitat origin and host phylogenetic relatedness. Mixed infections were widespread, with certain viruses exhibiting new host associations in this region.
Article
Agronomy
Tatsuya Kon, Noriko Watanabe, Hironori Ootake, Shin-ichi Fuji
Summary: Diseases associated with tomato spotted wilt orthotospovirus (TSWV) pose a serious threat to vegetable production worldwide. A new strain of TSWV, designated as TSWV-Fukushima:cucumber 2019 (TS-FC19), was identified as the causative agent of cucumber leaf necrosis disease in Fukushima Prefecture. This is the first detailed report on the biological characteristics of a cucumber-infecting TSWV.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Soledad de Breuil, Carolina Dottori, Nicolas Bejerman, Claudia Nome, Fabian Giolitti, Sergio Lenardon
Summary: This study investigated the prevalence of orthotospoviruses in peanut in Argentina, revealing a significant outbreak of GRSV for the first time and providing information on the occurrence and distribution of some Orthotospovirus species.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Entomology
Chahrazed Warda Halimi, Malik Laamari, Arturo Goldarazena
Summary: This study aimed to investigate the diversity of thrips species on olive trees in Algeria and their impact on fruit damage. The research findings revealed nine thrips species, with four being reported for the first time in Algeria. Neohydatothrips amygdali was the most common species in recent years, and its abundance was influenced by the phenological stages of the olive tree and environmental variations.
Article
Zoology
Shi-meng Zhang, Cheng-jun Ban, Ji-nian Feng, Mao -fa Yang
Summary: A new species of Neohydatothrips, N. stachyurus, is described from Guizhou, China. The identity of this new Sericothripinae species is confirmed based on its morphological characteristics, including the shape of the blotch on the pronotum and the distribution of microtrichia on the abdominal segments. The distribution of Neohydatothrips species from China is also discussed.
Editorial Material
Plant Sciences
Cullen Shaffer, Misaela Vakic, Ioannis E. Tzanetakis
Article
Plant Sciences
D. E. V. Villamor, K. E. Keller, R. R. Martin, I. E. Tzanetakis
Summary: This study compared virus detection using high throughput sequencing (HTS) and standard protocols in berry selections. HTS showed superior detection capabilities for variants and discovery of novel viruses. Additionally, the study found inconsistent results between the two protocols, suggesting a testing requirement of four sampling times over two growing seasons to ensure comprehensive virus detection.
Review
Microbiology
Fabio Pasin, Jose-Antonio Daros, Ioannis E. Tzanetakis
Summary: Potyviridae, one of the largest families of RNA viruses, has undergone notable evolutionary radiations accompanied by large genomic diversity and expansion of encoded protein modules. This family, belonging to the picorna-like supergroup, plays an important role in agriculture and ecology. Recent sequencing efforts have revealed a rich variability in gene content and genomic layouts among potyvirids. In this review, the heterogeneity of non-core modules that contribute to the structural and functional diversity of potyvirid proteomes is explored. The classification of P1 proteinases into functional Types A and B is provided, and various interesting non-core modules, such as sweet potato potyviral ORF (PISPO), putative zinc fingers, and alkylation B (AlkB), are discussed. The study also highlights the functional connections between these non-core modules and their role in host niche adaptation and immune evasion, driving the evolutionary radiation of Potyviridae. Finally, potential biotechnological and synthetic biology applications of potyvirid leader proteinases and non-core modules are explored.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Infectious Diseases
Tobiasz Druciarek, Mariusz Lewandowski, Ioannis Tzanetakis
Summary: This study examines the genetic diversity of eriophyoid fauna in rose rosette-affected hotspots and identifies a potentially new vector for the disease. Additionally, it emphasizes the importance of understanding the genetic diversity of eriophyoids inhabiting roses in North America for controlling the spread of the disease.
INFECTION GENETICS AND EVOLUTION
(2021)
Editorial Material
Plant Sciences
C. M. Shaffer, D. C. Michener, N. B. Vlasava, H. Chotkowski, M. Botermans, J. Starre, I. E. Tzanetakis
Article
Virology
Cullen M. Shaffer, David C. Michener, Nastassia B. Vlasava, Henry Chotkowski, Kurt Lamour, Daisy Stainton, Ioannis E. Tzanetakis
Summary: The Lychnis mottle virus (LycMoV) in peony is primarily spread through propagation material rather than active vector-mediated transmission. The population structure of the virus in the United States is homogenous, as shown by phylogenetic analyses of publicly available sequences.
Article
Virology
Cullen M. Shaffer, David C. Michener, Nastassia B. Vlasava, Henry Chotkowski, Ioannis E. Tzanetakis
Summary: Cycas necrotic stunt virus (CNSV) is rapidly spreading in agricultural crops globally, including peony. Understanding the evolution and epidemiology of CNSV is crucial. This study developed a multiplex RT-PCR detection protocol to accurately detect the virus and protect its hosts.
Article
Plant Sciences
D. E. V. Villamor, A. Sierra Mejia, R. R. Martin, I. E. Tzanetakis
Summary: A new blueberry virus, tentatively named blueberry virus S (BluVS), was discovered and classified as a distinct species within the genus Carlavirus (family Betaflexiviridae) based on sequence identity, phylogenetics, and serological and biological properties. The virus genome was extensively analyzed and an infectious clone was developed for studying virus pathogenicity. The BluVS caused severe systemic symptoms in Nicotiana occidentalis and showed symptomless infection in Chenopodium quinoa, indicating its distinct speciation. The availability of an infectious clone provides valuable tools for future studies on the biology of the virus.
Article
Plant Sciences
Ava N. Medberry, Ashish Srivastava, Alfredo Diaz-Lara, Maher Al Rwahnih, Dan E. V. Villamor, Ioannis E. Tzanetakis
Summary: Strawberry (Fragaria x ananassa) is a global important berry crop that is constantly threatened by viruses. A novel virus called strawberry virus 3 (StrV-3) was discovered, which does not show significant similarity with known rhabdoviruses, suggesting the establishment of a new genus. A triplex reverse-transcription PCR test was developed for detecting the virus in the National Clonal Germplasm Repository Fragaria collection. A CRISPR-Cas-based protocol was also developed for detecting the virus in candidate G1 plants. The potential of the strawberry aphid (Chaetosiphon fragaefolii) as a vector of the virus was evaluated, but with no success. This work enhances our knowledge of the Rhabdoviridae family and aids in the goal of releasing virus-free plant materials.
Article
Genetics & Heredity
Misaela Vakic, Daisy Stainton, Duska Delic, Ioannis E. Tzanetakis
Summary: This article reports the first episomal genome of RYNV isolated from blackberry and fills in some gaps in RYNV genetics.
Article
Plant Sciences
Ava Medberry, Ioannis E. Tzanetakis
Summary: In 2020, a novel virus closely related to lettuce necrotic yellows virus, named strawberry virus 2 (StrV-2), was discovered in strawberry and studied in-depth. The complete StrV-2 genome was obtained and investigated in silico. Transmission was assessed using two aphid species, and a multiplex RT-PCR test was developed for screening the NCGR Fragaria virus collection.
Article
Biotechnology & Applied Microbiology
Joao Marcos Fagundes Silva, Fernando Lucas Melo, Santiago F. Elena, Thierry Candresse, Sead Sabanadzovic, Ioannis E. Tzanetakis, Arnaud G. Blouin, Dan Edward Veloso Villamor, Dimitre Mollov, Fiona Constable, Mengji Cao, Pasquale Saldarelli, Won Kyong Cho, Tatsuya Nagata
Summary: Currently, virus classification is primarily based on genome organization and sequence similarities of capsid and replication-associated proteins. However, for many newly identified viruses, information on biological traits is limited. This paper critically discusses the classification of viruses in the Betaflexiviridae family in the era of high-throughput sequencing and proposes updated criteria based on pairwise identity analyses and phylogenetics to address current issues and facilitate future virus classification.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Plant Sciences
Tobiasz Druciarek, Mariusz Lewandowski, Ioannis Tzanetakis
Summary: Rose rosette disease is devastating the ornamentals industry in the United States, with rose rosette emaravirus (RRV) being the cause and the eriophyoid mite Phyllocoptes fructiphilus (Acari: Eriophyoidea) acting as the vector. This study investigates the vector competence and transmission efficiencies of two other Phyllocoptes species, P. adalius and P. arcani, in single and multiple mite transfer experiments. The identification of P. arcani as a second vector of RRV is significant for the epidemiology of the disease, as it may be present in plants where P. fructiphilus is absent.
Editorial Material
Plant Sciences
Ashish Srivastava, Ioannis E. Tzanetakis
Article
Virology
Daisy Stainton, Dan E. V. Villamor, Andrea Sierra Mejia, Ashish Srivastava, Dimitre Mollov, Robert R. Martin, Ioannis E. Tzanetakis
Summary: Screening through high throughput sequencing identified a new virus, named blueberry virus L (BlVL), in blueberry accessions. BlVL shows genomic structure and sequence similarity to nectarine stem pitting associated virus (NSPaV). Analysis revealed that BlVL, like NSPaV, lacks identifiable movement proteins. Screening of over 600 samples from five states found that 79% were infected, making BlVL the most widespread blueberry virus in the United States.
