Review
Genetics & Heredity
Veena Unnikrishnan, John Kastelic, Jacob Thundathil
Summary: In cattle, the efficiency of ICSI is hindered by failed oocyte activation post sperm injection. Research focusing on proteins like Na/K-ATPase and PLC zeta is key to improving the success rate. Enhancing the efficiency of bovine ICSI has significant implications for the cattle breeding industry.
Article
Obstetrics & Gynecology
Takashi Shibahara, Yuu Fukasaku, Nozomi Miyazaki, Hiroaki Kawato, Hiroyuki Minoura
Summary: This study evaluated the effectiveness of expanding the indications of early rescue ICSI and found that by expanding the indications to include oocytes with <80% rate of the second polar body extrusion, more fertilized oocytes can be obtained without significantly increasing the incidence of abnormal fertilization.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2022)
Review
Endocrinology & Metabolism
Yamei Xue, Xiaohong Cheng, Yuping Xiong, Kun Li
Summary: This review summarizes and discusses the mutations of nine important genes associated with fertilization failure in infertile patients. The review highlights their roles in the fertilization process and suggests these mutations as potential targets for precision treatments in reproductive medicine. Furthermore, the review provides helpful clues for genetic counseling and optimizing clinical treatments for human infertility by supplying timely information on the genetic causes of fertilization failure.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Luisa Ramirez-Agamez, Camilo Hernandez-Aviles, Dickson D. Varner, Charles C. Love
Summary: This study explores stallion-associated factors in intracytoplasmic sperm injection (ICSI) using frozen/thawed sperm. The results indicate that the composition of freezing extender affects the developmental competence of sperm and in vitro-matured equine oocytes after ICSI. Furthermore, there were no significant differences observed when using ejaculated or epididymal sperm, or sperm with different morphological features in ICSI outcomes.
Article
Genetics & Heredity
Takashi Shibahara, Yuu Fukasaku, Naoko Hayashi, Nozomi Miyazaki, Hiroaki Kawato, Hiroyuki Minoura
Summary: The study suggests that early ROA after ICSI is effective in rescuing unfertilized oocytes, with a higher normal fertilization rate compared to non-ROA oocytes. However, the blastocyst development rate is lower, while the implantation rate is similar to spontaneously activated oocytes.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Hye Gyeong Jeong, Seul Ki Kim, Jangmi Lee, Hye Won Youm, Byung Chul Jee
Summary: This study found that embryo quality is more closely associated with oolemma resistance during ICSI. The newly developed oolemma score helps in identifying the embryo developmental potential of each mature oocyte in ICSI cycles.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2021)
Review
Andrology
Ashok Agarwal, Rakesh Sharma, Sajal Gupta, Renata Finelli, Neel Parekh, Manesh Kumar Panner Selvam, Ralf Henkel, Damayanthi Durairajanayagam, Camila Pompeu, Sarah Madani, Andrea Belo, Neha Singh, Simryn Covarrubias, Sara Darbandi, Raha Sadeghi, Mahsa Darbandi, Paraskevi Vogiatzi, Florence Boitrelle, Mara Simopoulou, Ramadan Saleh, Mohamed Arafa, Ahmad Majzoub, Hussein Kandil, Armand Zini, Edmund Ko, Juan G. Alvarez, Marlon Martinez, Jonathan Ramsay, Sunil Jindal, Gian Maria Busetto, Hassan Sallam, Israel Maldonado, Christina Anagnostopoulou, Marco G. Alves, Pallav Sengupta, Kambiz Gilany, Donald P. Evenson, Sheena E. M. Lewis, Jaime Gosalvez, Rafael F. Ambar, Rupin Shah
Summary: Semen analysis is the first step in evaluating male fertility, but sperm morphology assessment is difficult to standardize and obtain reproducible results. Standardization of laboratory protocols is crucial in ensuring reliable laboratory results.
WORLD JOURNAL OF MENS HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Maryam Jenabi, Parvin Khodarahmi, Farzaneh Tafvizi, Saeed Zaker Bostanabad
Summary: This study investigated the expression levels of CXCL8 in serum, follicular fluid, and cumulus cells, and its relationship with oocyte maturation and embryo quality. The study found that CXCL8 protein and mRNA levels were higher in women with female factor infertility, and were inversely related to oocyte maturation. CXCL8 concentrations in serum and follicular fluid may be potential non-invasive biomarkers for predicting oocyte maturation outcome.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Obstetrics & Gynecology
Takuma Sato, Hiroshi Kishi, Saori Murakata, Yuki Hayashi, Toshiyuki Hattori, Shinji Nakazawa, Yusuke Mori, Miwa Hidaka, Yuta Kasahara, Atsuko Kusuhara, Kayo Hosoya, Hiroshi Hayashi, Aikou Okamoto
Summary: The purpose of this study was to create and evaluate a machine-learning model for YOLOv3 that can simultaneously perform morphological evaluation and tracking in a short time, which can be adapted to video data under an inverted microscope.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Ahmad Aboukhshaba, Nahid Punjani, Sofia Doukakis, Nikica Zaninovic, Gianpiero Palermo, Peter N. Schlegel
Summary: The study aimed to evaluate the impact of sperm characteristics on intracytoplasmic sperm injection (ICSI) outcomes in men with nonobstructive azoospermia (NOA) following microdissection testicular sperm extraction (mTESE). The results showed that specific sperm defects did not completely preclude the chance of clinical pregnancy. This study is the first comprehensive evaluation of the importance of these characteristics in ICSI outcomes.
FERTILITY AND STERILITY
(2022)
Article
Obstetrics & Gynecology
Robert John Norman
Summary: Intracytoplasmic sperm injection (ICSI) was initially introduced for male infertility but is now widely used in assisted reproduction techniques. The indications for ICSI use need to be re-evaluated to ensure appropriate application in infertility management.
FERTILITY AND STERILITY
(2022)
Article
Medicine, General & Internal
Yun-Qiao Dong, Chuang-Qi Chen, Yu-Qiang Huang, Dun Liu, Xi-Qian Zhang, Feng-Hua Liu
Summary: In this study, the effect of sperm on the developmental potential of in vitro-matured oocytes in conventional culture was investigated. The rates of in vitro oocyte maturation, normal fertilization, normal cleavage, day-3 top-quality embryos, and useful blastocyst formation were compared between the old sperm group and the fresh sperm group.
WORLD JOURNAL OF CLINICAL CASES
(2022)
Article
Endocrinology & Metabolism
Ziwen Xu, Guidong Yao, Wenbin Niu, Huiying Fan, Xueshan Ma, Senlin Shi, Haixia Jin, Wenyan Song, Yingpu Sun
Summary: The study found that using calcium ionophore (A23187) for rescue activation of unfertilized oocytes can obtain a certain proportion of high-quality blastocysts with normal karyotype, and it does not significantly affect the timing of embryo development. In clinical practice, direct activation with calcium ionophore (A23187) after ICSI is more effective than rescue activation the next day.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Veterinary Sciences
Effrosyni Fatira, Milos Havelka, Taiju Saito, Jose Landeira, Marek Rodina, David Gela, Martin Psenicka
Summary: In this study, the researchers applied intracytoplasmic sperm injection (ICSI) technology to endangered sturgeons and found that it successfully produced embryos and larvae. Furthermore, molecular analysis showed genome integration of both egg and sperm donor species, demonstrating the potential of ICSI for assisted reproduction in sturgeons.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Andrology
Kareim Khalafalla, Ahmad Majzoub, Haitham Elbardisi, Ashni Bhathella, Arth Chaudhari, Ashok Agarwal, Ralf Henkel, Thoraya AlMarzooki, Hasan Burjaq, Mohamed Arafa
Summary: This study aimed to assess the impact of different levels of sperm DNA fragmentation on the outcome of clinical intracytoplasmic sperm injection. Results showed that in cases where sperm DNA fragmentation exceeded 30%, favorable females with older age or lower anti-Mullerian hormone levels had significantly higher clinical pregnancy and live birth rates compared to unfavorable females.