Article
Immunology
Zhuolun Sun, Changying Jing, Hailun Zhan, Xudong Guo, Ning Suo, Feng Kong, Wen Tao, Chutian Xiao, Daoyuan Hu, Hanbo Wang, Shaobo Jiang
Summary: In this study, potential antigens for mRNA-based vaccines against bladder urothelial carcinoma (BLCA) were identified. Furthermore, two immune clusters were identified, with patients in IC2 potentially benefiting more from vaccination.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Thomas van Doeveren, Jose A. Nakauma-Gonzalez, Andrew S. Mason, Geert J. L. H. van Leenders, Tahlita C. M. Zuiverloon, Ellen C. Zwarthoff, Isabelle C. Meijssen, Angelique C. van der Made, Antoine G. van Der Heijden, Kees Hendricksen, Bas W. G. van Rhijn, Charlotte S. Voskuilen, Job van Riet, Winand N. M. Dinjens, Hendrikus J. Dubbink, Harmen J. G. van de Werken, Joost L. Boormans
Summary: By sequencing genes in 15 UTUC patients treated by RNU, it was found that the majority of patients had a clonal relationship between UTUC and subsequent UCB, supporting the idea that UCBs following UTUC surgery are primarily clonally derived recurrences.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Oncology
Gorka Larrinaga, Julio Calvete-Candenas, Jon Danel Solano-Iturri, Ana M. Martin, Angel Pueyo, Caroline E. Nunes-Xavier, Rafael Pulido, Juan F. Dorado, Jose I. Lopez, Javier C. Angulo
Summary: This study provides a novel description of the (Pro)renin receptor (PRR) protein and its prognostic role in invasive urothelial cancer of the bladder. It demonstrates that PRR expression is an independent prognostic marker and may be a potential target in urothelial carcinoma. The study suggests that PRR activates pathogenic pathways contributing to cell proliferation, immunosuppressive microenvironments, and aggressive neoplastic phenotypes.
Article
Mathematical & Computational Biology
Peiyuan Li, Gangjie Qiao, Jian Lu, Wenbin Ji, Chao Gao, Feng Qi
Summary: PVT1 plays a crucial role in the progression and prognosis of bladder urothelial carcinoma (BLCA), with high expression associated with lower survival rates. It serves as an independent prognostic factor and can be used as a medium biomarker to predict survival after cystectomy.
MATHEMATICAL BIOSCIENCES AND ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Lucia Schwarzova, Zuzana Varchulova Novakova, Lubos Danisovic, Stanislav Ziaran
Summary: Urothelial bladder carcinoma is one of the top ten most commonly diagnosed cancers worldwide. The classification system based on mRNA expression profiling revealed the existence of molecular subtypes of bladder cancer, which can provide more precise stratification of patients and impact treatment decisions and outcomes.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Cell Biology
Minhua Deng, Ning Wang, Zhiyong Li, Rixin Chen, Jinling Duan, Yulu Peng, Zeshen Wu, Zhiling Zhang, Lijuan Jiang, Xianchong Zheng, Dan Xie, Wensu Wei, Zhuowei Liu, Fangjian Zhou
Summary: RNA-binding proteins (RBPs), including Fragile X-related gene 1 (FXR1), play important regulatory roles in gene expression. Dysregulation of RBPs, such as overexpression of FXR1, can promote urothelial carcinoma of the bladder (UCB) proliferation and tumorigenesis by affecting the 3' processing of TRAF1 mRNA. Understanding the novel regulatory role of FXR1 in UCB oncogenesis provides new insight into the function of RBPs and potential therapeutic targets for UCB.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Jian Hou, Zhenquan Lu, Xianhua Liu, Bingfeng Luo, Genyi Qu, Yong Xu, Cheng Tang
Summary: The expression of nucleolar and spindle associated protein 1 (NUSAP1) is associated with bladder urothelial carcinoma (BUC) tissues, lymph node metastasis, and survival prognosis. High NUSAP1 expression is significantly correlated with pathological stage, pathological grade, tumor diameter, and lymph node metastasis. NUSAP1 is an independent predictor of lymph node metastasis and prognosis in BUC.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Ning Xu, Zhenmei Yao, Guoguo Shang, Dingwei Ye, Haixing Wang, Hailiang Zhang, Yuanyuan Qu, Fujiang Xu, Yunzhi Wang, Zhaoyu Qin, Jiajun Zhu, Fan Zhang, Jinwen Feng, Sha Tian, Yang Liu, Jianyuan Zhao, Jun Hou, Jianming Guo, Yingyong Hou, Chen Ding
Summary: This study provides an integrated multi-omics analysis of Chinese UC patients, revealing the association between SND1 and CDK5 amplifications and the activation of STAT3 in tumor proliferation. It also identifies chromosome 5p gain as a high-risk factor in NMIBC patients through actin cytoskeleton modulation. Phosphoproteomic analysis identifies UC-associated activated kinases, and proteomic analysis groups UC patients into distinct prognostic and molecular signature categories. Immune subtypes of UC tumors demonstrate a complex immune landscape with the amplification of TRAF2 related to increased PD-L1 expression. Lastly, GARS is validated to promote the pentose phosphate pathway by inhibiting activities of PGK1 and PKM2 in U-II subtype.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Article
Oncology
Kaiwei Yang, Wei Yu, Huanhuan Liu, Feng Ding, Yanrui Zhang, Yun Zhang, Wang Wang, Feng Lou, Shanbo Cao, Huina Wang, Zhisong He
Summary: The study revealed significant genomic differences and carcinogenesis prevalence between UTUC and UCB, with differences in mutational landscape and driver genes. This suggests the necessity for site-specific management of patients with urothelial carcinoma. Mutational signatures could serve as screening tools for clinical differential diagnosis between UTUC and UCB.
Article
Oncology
Junjie Zhang, Ran Xu, Qiang Lu, Zhenzhou Xu, Jianye Liu, Pei Li, Yaqun Zhang, Chuanchi Zhou, Lufeng Luo, Wei Tang, Zhenting Wang, Manman Cao, Jian Cao, Genming Xu, Long Wang
Summary: In this study, a methylation diagnostic tool using urine sediment was constructed for the detection of urothelial bladder carcinoma (UBC), and the diagnostic performance was improved by incorporating single nucleotide polymorphism sites. The model achieved high sensitivity and specificity, and showed comparable performance to conventional cytology and FISH. It has the potential to be used as a replacement approach for detecting UBC.
Article
Oncology
So Young Kang, You Jeong Heo, Ghee Young Kwon, Kyoung-Mee Kim
Summary: This study aimed to test the feasibility of using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to measure PD-L1 (CD274) mRNA expression levels and compared the results with PD-L1 IHC. The results showed a good correlation between CD274 mRNA levels and PD-L1 expression, and patients with high CD274 mRNA levels had better responses to ICI treatment.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Xia Wang, Jin Wang, Yu-Man Tsui, Chaoran Shi, Ying Wang, Xin Zhang, Qian Yan, Miao Chen, Chen Jiang, Yun-Fei Yuan, Chun-Ming Wong, Ming Liu, Zeng-yu Feng, Honglin Chen, Irene Oi Lin Ng, Lingxi Jiang, Xin-Yuan Guan
Summary: Studies have shown that RALYL, a liver progenitor specific gene, regulates HCC stemness by sustaining TGF-beta 2 mRNA stability, which may inspire precise therapeutic strategies for HCC.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Alberto Valdes, Athanasios Bitzios, Eszter Kassa, Ganna Shevchenko, Alexander Falk, Per-Uno Malmstrom, Anca Dragomir, Ulrika Segersten, Sara Bergstrom Lind
Summary: Samples preserved by different methods, FFPE and OCT, show differences in protein analysis results using MS technology, with preservation method being a main source of variation. Proteins related to mitochondrial function are overrepresented in OCT data but missing in FFPE data. The use of MS technology for biobank samples is supported and further evaluation of proteins as biomarkers is encouraged.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Junjie Ji, Yu Yao, Lijiang Sun, Qingya Yang, Guiming Zhang
Summary: This study aimed to develop and validate a nomogram for preoperatively predicting lymph node metastasis in bladder urothelial carcinoma patients. Patient data including demographics, pathology, imaging, and laboratory data were collected. Independent preoperative risk factors were identified and used to develop the nomogram. The nomogram showed good predictive accuracy and clinical applicability in both internal and external validation.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Oncology
Gottfrid Sjodahl, Pontus Eriksson, Karin Holmsten, Johan Abrahamsson, Mattias Hoglund, Carina Bernardo, Anders Ullen, Fredrik Liedberg
Summary: This study finds that molecular subtypes of urothelial bladder cancer are associated with specific metastatic sites. The Basal/squamous subtype is less likely to metastasize to bone, while the Urothelial-like subtype has an increased tendency to metastasize to bone. The Genomically unstable subtype is less likely to metastasize to the lungs, but is more likely to metastasize to atypical sites, including the brain.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Gastroenterology & Hepatology
Francesco Vitali, Nadine Morgenstern, Markus Eckstein, Raja Atreya, Maximilian Waldner, Arndt Hartmann, Markus F. Neurath, Timo Rath
Summary: This study aimed to evaluate the use of endocytoscopy (EC) to accurately assess histologic inflammation and predict disease progression in ulcerative colitis (UC) patients. The results showed that EC assessment of inflammatory activity in UC was strongly correlated with histopathologic scoring and superior to white-light endoscopy. Therefore, EC can provide real-time histology in UC patients and better predict clinical outcomes.
GASTROINTESTINAL ENDOSCOPY
(2023)
Review
Urology & Nephrology
Christian Bolenz, Frank Kunath, Friedemann Zengerling, Felix Wezel, Stefanie Schmidt, Arndt Hartmann, Markus Eckstein
Summary: This study evaluated the role of tissue biomarker testing in interventional clinical trials for urothelial carcinoma (UC). The number of studies using biomarker-guided interventions for advanced UC has increased over the past 25 years. However, research on druggable targets in actual UC biopsies before treatment is still limited, highlighting the need for standardized criteria for tissue-based biomarker testing to accelerate personalized treatment.
UROLOGIA INTERNATIONALIS
(2023)
Article
Multidisciplinary Sciences
Miriam Saponaro, Sina Flottmann, Markus Eckstein, Oliver Hommerding, Niklas Kluemper, Dillon Corvino, Sana Hosni, Anja Schmidt, Nicolas Moenig, Doris Schmidt, Joerg Ellinger, Marieta Toma, Glen Kristiansen, Tobias Bald, Andrea Alimonti, Manuel Ritter, Michael Hoelzel, Abdullah Alajati
Summary: The poor prognosis of advanced urothelial carcinoma (UC) and the need for improved treatment have led researchers to investigate the role of CUB domain containing protein 1 (CDCP1) in UC. They found that CDCP1 is highly expressed in advanced UC and its overexpression is associated with shorter overall survival. Experiments with organoids and cell lines demonstrated that CDCP1 plays an oncogenic role by activating the MAPK/ERK pathway and promoting proliferation and migration. Targeting CDCP1 could be a rational therapeutic strategy for advanced UC.
SCIENTIFIC REPORTS
(2023)
Article
Urology & Nephrology
Francesco Claps, Maaike W. van de Kamp, Roman Mayr, Peter J. Bostrom, Shahrokh F. Shariat, Katrin Hippe, Simone Bertz, Yann Neuzillet, Joyce Sanders, Wolfgang Otto, Michiel S. van Der Heijden, Michael A. S. Jewett, Robert Stoehr, Alexandre R. Zlotta, Carlo Trombetta, Markus Eckstein, Laura S. Mertens, Maximilian Burger, Yanish Soorojebally, Bernd Wullich, Riccardo Bartoletti, Francois Radvanyi, Nicola Pavan, Nanour Sirab, M. Carmen Mir, Damien Pouessel, Theo H. van Der Kwast, Arndt Hartmann, Yair Lotan, Rossana Bussani, Yves Allory, Bas W. G. van Rhijn
Summary: The study aims to evaluate variant histologies (VHs) for disease-specific survival (DSS) in patients with invasive urothelial bladder cancer (BCa) undergoing radical cystectomy (RC). The results showed that compared to pure urothelial carcinoma, clear-cell, plasmacytoid, small-cell, and sarcomatoid VHs were associated with worse DSS, while lymphoepithelioma-like VH showed a DSS benefit. Accurate pathological diagnosis can ensure tailored counselling for patients who require more intensive management.
Editorial Material
Urology & Nephrology
Niklas Kluemper, Markus Eckstein, Michael Hoelzel, Ken Herrmann, Boris Hadaschik, Viktor Gruenwald
Letter
Urology & Nephrology
Niklas Kluemper, Alexander Cox, Markus Eckstein
Article
Biochemistry & Molecular Biology
Christian Soehngen, David J. Thomas, Margaretha A. Skowron, Felix Bremmer, Markus Eckstein, Anja Stefanski, Marc D. Driessen, Gamal A. Wakileh, Kai Stuehler, Peter Altevogt, Dan Theodorescu, Ruediger Klapdor, Axel Schambach, Daniel Nettersheim
Summary: This study aimed to understand the molecular function of CD24 in vitro and evaluate the cytotoxic capacity of NK cell CAR against CD24 in urological tumor cells. The results showed that CD24 interacts with proteins involved in cell adhesion, ATP binding, phosphoprotein binding, and post-translational modifications. Treatment with NK-CD24-CAR cells significantly decreased cell viability and induced apoptosis specifically in CD24(+) tumor cells. This study provides a promising novel target for immune therapeutic approaches against urological malignancies.
Article
Oncology
Raphela A. Ranjan, Julienne K. Muenzner, Philipp Kunze, Carol I. Geppert, Matthias Ruebner, Hanna Huebner, Peter A. Fasching, Matthias W. Beckmann, Tobias Baeuerle, Arndt Hartmann, Wolfgang Walther, Markus Eckstein, Ramona Erber, Regine Schneider-Stock
Summary: The biological and growth characteristics of two breast cancer cell lines were investigated in a CAM model, and the results confirmed the known features of the breast cancer cell lines observed in vitro and in cells. The CAM model was found to be a reliable alternative for breast cancer research, reducing the need for murine animal experiments.
Article
Engineering, Biomedical
Despina Soteriou, Marketa Kubankova, Christine Schweitzer, Rocio Lopez-Posadas, Rashmita Pradhan, Oana-Maria Thoma, Andrea-Hermina Gyoerfi, Alexandru-Emil Matei, Maximilian Waldner, Joerg H. W. Distler, Stefan Scheuermann, Jens Langejuergen, Markus Eckstein, Regine Schneider-Stock, Raja Atreya, Markus F. Neurath, Arndt Hartmann, Jochen Guck
Summary: A method for rapid diagnosis of tissue biopsies by assessing the physical phenotypes of singularized suspended cells. This method combines enzyme-free mechanical dissociation of tissues, real-time deformability cytometry, and data analysis to detect pathological changes in solid biopsies.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Medicine, General & Internal
Frank Waldbillig, Felix Bormann, Manuel Neuberger, Jorg Ellinger, Philipp Erben, Maximilian C. C. Kriegmair, Maurice Stephan Michel, Philipp Nuhn, Malin Nientiedt
Summary: This study conducted a transcriptome-wide m6A sequencing analysis of ccRCC and validated the expression of 35 preidentified m6A targets. The results showed dysregulation of m6A-driven genes in ccRCC, which were associated with patient survival and functional impact. These findings reveal the potential role of m6A in ccRCC and provide insights for developing novel therapies and identifying prognostic markers.
Article
Oncology
Jonas Saal, Tobias Bald, Markus Eckstein, Manuel Ritter, Peter Brossart, Joerg Ellinger, Michael Hoelzel, Niklas Kluemper
Summary: This study validated the high predictive value of early C-reactive protein (CRP) kinetics in patients with non-small cell lung cancer undergoing immune checkpoint inhibition treatment. The use of this cost-effective and easy-to-implement on-treatment biomarker was justified to optimize therapy monitoring.
JNCI CANCER SPECTRUM
(2023)
Article
Otorhinolaryngology
Matthias Balk, Robin Rupp, Matti Sievert, Konstantinos Mantsopoulos, Moritz Allner, Philipp Grundtner, Sarina K. K. Mueller, Markus Eckstein, Heinrich Iro, Markus Hecht, Antoniu-Oreste Gostian
Summary: The study aimed to determine if HPV-HNCUP and HPV-OPSCC are the same disease. Propensity score matching was used to compare the oncological outcomes of both groups. The results showed that HPV-HNCUP and HPV-OPSCC are two different entities with different oncological outcomes, as evidenced by significantly lower overall survival and disease specific survival rates in HPV-HNCUP patients.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Immunology
Tina Daunke, Silje Beckinger, Sascha Rahn, Sandra Kruger, Steffen Heckl, Heiner Schafer, Daniela Wesch, Christian Pilarsky, Markus Eckstein, Arndt Hartmann, Christoph Rocken, Anna Maxi Wandmacher, Susanne Sebens
Summary: In pancreatic ductal adenocarcinoma (PDAC), tumor-associated macrophages express PD-L1 while PDAC cells do not. PD-L1 expression is high at the tumor invasion front between tumor-associated macrophages and CD8+ T cells. Treatment with immune checkpoint inhibitors (ICI) does not enhance the activation and cytotoxicity of CD8+ T cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Elias A. T. Koch, Anne Petzold, Anja Wessely, Edgar Dippel, Markus Eckstein, Anja Gesierich, Ralf Gutzmer, Jessica C. Hassel, Harald Knorr, Nicole Kreuzberg, Ulrike Leiter, Carmen Loquai, Friedegund Meier, Markus Meissner, Peter Mohr, Claudia Pfoehler, Farnaz Rahimi, Dirk Schadendorf, Max Schlaak, Kai-Martin Thoms, Selma Ugurel, Jochen Utikal, Michael Weichenthal, Beatrice Schuler-Thurner, Carola Berking, Markus V. Heppt
Summary: Metastases of uveal melanoma predominantly spread to the liver. Liver-directed therapies (LDT) are commonly used for tumor control in patients with low response rates to systemic therapies. This study compared the outcomes of patients with metastatic UM treated with immune checkpoint blockade (ICB), with and without LDT, and found that the combination of LDT with ICB was associated with longer overall survival, improved progression-free survival, and higher treatment response rates.
FRONTIERS OF MEDICINE
(2023)
Article
Health Policy & Services
Raymond H. Henderson, Declan French, Elaine Stewart, Dave Smart, Adam Idica, Sandra Redmond, Markus Eckstein, Jordan Clark, Richard Sullivan, Peter Keeling, Mark Lawler
Summary: Precision oncology medicines have lower development and market costs compared to non-precision oncology medicines, and they have a higher return on investment.
JOURNAL OF PHARMACEUTICAL POLICY AND PRACTICE
(2023)
