Article
Agriculture, Dairy & Animal Science
Ziyong Xu, Mei Li, Wang Lu, Liping Wang, Yujuan Zhang
Summary: This study investigates the role of chicken xenobiotic receptor (CXR) in the regulation of breast cancer resistance protein (BCRP). The results show that CXR is involved in the regulation of BCRP gene expression and protein activity in chickens. Activation of CXR by metyrapone upregulates BCRP expression and transporter activity. Co-administration of metyrapone affects the pharmacokinetic behavior of orally administered BCRP substrate.
Article
Chemistry, Applied
Quanliang Li, Xiyu Wang, Xiaomeng Wang, Lin Zheng, Ping Chen, Biying Zhang
Summary: Detrimental contamination of zearalenone (ZEN) in crops and foodstuffs poses an ongoing threat to global food security and human health. Conventional detection methods for ZEN have deficiencies, while nanomaterial-based biosensors have emerged as superior substitutes due to their high sensitivity, portability, and miniaturization. This review provides an overview of the fabrication strategies and applications of nanomaterial-based biosensors for ZEN detection, highlighting sensing mechanisms, response types, and analytical performance as well as the integration of diverse recognition elements to enhance sensing capabilities. Critical challenges and future perspectives for expedited ZEN detection are also discussed.
Article
Biochemistry & Molecular Biology
Julia Kowal, Dongchun Ni, Scott M. Jackson, Ioannis Manolaridis, Henning Stahlberg, Kaspar P. Locher
Summary: In this study, three cryo-EM structures of human ABCG2 bound to anticancer drugs tariquidar, topotecan, and mitoxantrone were presented, revealing the binding pocket details and functional importance of key residues. Mutagenesis experiments and 3D variability analysis provided further insights into the mechanisms by which ABCG2 distinguishes between inhibitors and substrates.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Zsolt Safar, Gabor Kecskemeti, Judit Molnar, Anita Kurunczi, Zoltan Szabo, Tamas Janaky, Emese Kis, Peter Krajcsi
Summary: BCRP/ABCG2 is a key factor in the pharmacokinetics of substrate drugs. Membranes from different expression systems show variation in substrate correlation and dynamic range, but good consistency in IC50 values.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Laura Suominen, Noora Sjostedt, Kati-Sisko Vellonen, Mikko Gynther, Seppo Auriola, Heidi Kidron
Summary: Reducing the activity of ABCG2 transporter can lead to increased drug absorption and plasma levels. The Q141K variant of ABCG2 has a clinically significant effect of decreasing protein levels and transport activity. In addition, there are over 500 rare variants of ABCG2, but their functionality is still unknown. This study identified 14 rare variants that have a decreased function phenotype, potentially leading to increased drug exposure.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Analytical
Weili Qiao, Baoshan He, Wenjie Ren, Renyong Zhao, Zhiguang Suo, Haoyang Yan, Yiwei Xu, Min Wei, Huali Jin
Summary: In this study, colloidal Au spheres and Au nanoflowers were used as signal labels to detect ZEN in cereals. The sensitivity of the immunochromatographic strip was optimized by adjusting the volume of antigen, antibody, and probe quantities. The use of Au nanoflowers as signal labels showed higher sensitivity compared to traditional colloidal Au spheres, with a low limit of detection of 0.08 ng mL(-1). The Au nanoflowers immunochromatographic strip demonstrated high specificity and sensitivity for ZEN detection.
ANALYTICAL METHODS
(2022)
Article
Food Science & Technology
Krisztina Majer-Baranyi, Attila Barocsi, Patrik Gadoros, Laszlo Kocsanyi, Andras Szekacs, Nora Adanyi
Summary: A capillary-based immunofluorescence sensor was developed and integrated into a flow injection analysis system. The system achieved high sensitivity by efficiently collecting and detecting non-guided fluorescence signals scattered along the capillary wall. The sensor demonstrated excellent performance in quantitative determination, with low detection limits and a wide dynamic detection range.
Article
Food Science & Technology
Hyeon-Ju Lee, Se-Young Oh, Inho Jo
Summary: This study found that Zearalenone induces endothelial cell apoptosis through activation of an ERK1/2/p53/caspase 3 signaling pathway via release of Ca2+ from the endoplasmic reticulum, independent of reactive oxygen species production and estrogen receptor activation.
Review
Food Science & Technology
Karolina Ropejko, Magdalena Twaruzek
Summary: Mycotoxins are common food contaminants produced by filamentous fungi, with Zearalenone being one of over 400 detected toxins. Zearalenone, mainly produced by Fusarium fungi, has estrogenic effects and can lead to economic losses and pose risks to animals and humans. Further research and monitoring are needed to address the impact of mycotoxins on the food chain.
Article
Food Science & Technology
Hongxia Tan, Yao Li, Hongyuan Zhou, Ting Guo, Ying Zhou, Yuhao Zhang, Liang Ma
Summary: This study aimed to analyze the key factors inducing the interconversion of hidden ZEN with free ZEN during maize processing. The results showed that thermal and alkaline processing converted hidden ZEN into free ZEN, while acid processing converted free ZEN into hidden ZEN. The temperature and pH-induced interconversion rules were also applicable to practical maize processing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Congning Zhai, Yangguang Yu, Jun Han, Junqiang Hu, Dan He, Hongyin Zhang, Jianrong Shi, Sherif Ramzy Mohamed, Dawood H. Dawood, Gang Wang, Jianhong Xu
Summary: In this study, an anaerobic ZEN-degrading bacterium named F39 was isolated from animal intestinal contents and identified as Clostridium sporogenes. F39 showed a high efficiency in degrading ZEN and converting it into alpha- and beta-zearalenol, reducing the toxicity of degradation products. Additionally, F39 exhibited higher degradation efficiency in wheat-based feedstuffs compared to other feedstuffs, and the efficiency was pH related.
Article
Food Science & Technology
Xing Qin, Yanzhe Xin, Xiaoyun Su, Xiaolu Wang, Yaru Wang, Jie Zhang, Tao Tu, Bin Yao, Huiying Luo, Huoqing Huang
Summary: A novel mycotoxin-degrading enzyme StDyP was discovered in this study, which could degrade a certain amount of ZEN and combine the catalytic properties of manganese peroxidase and laccase to significantly accelerate the enzymatic degradation of ZEN. These findings may help improve the efficiency of mycotoxin degradation in food and feed.
Article
Food Science & Technology
Po-Yen Lee, Ching-Chih Liu, Shu-Chi Wang, Kai-Yin Chen, Tzu-Chieh Lin, Po-Len Liu, Chien-Chih Chiu, I-Chen Chen, Yu-Hung Lai, Wei-Chung Cheng, Wei-Ju Chung, Hsin-Chih Yeh, Chi-Han Huang, Chia-Cheng Su, Shu-Pin Huang, Chia-Yang Li
Summary: ZEA suppresses macrophage-mediated innate immunity activated by LPS, attenuating the production of proinflammatory mediators and cytokines, inhibiting inflammatory signaling pathways, and repressing inflammasome activation. This suggests that ZEA-contaminated food intake may decrease innate immunity and increase adverse effects during infection.
Article
Food Science & Technology
Xing Qin, Yanzhe Xin, Jiahuan Zou, Xiaoyun Su, Xiaolu Wang, Yaru Wang, Jie Zhang, Tao Tu, Bin Yao, Huiying Luo, Huoqing Huang
Summary: The study identified and cloned a novel MCO-encoding gene, StMCO, from Streptomyces thermocarboxydus, which showed potential in degrading various substrates and mycotoxins directly. This suggests that MCOs may be effective tools for degrading contaminated food products.
Review
Engineering, Chemical
Na Wu, Wen Ou, Zhidong Zhang, Yuwen Wang, Qing Xu, He Huang
Summary: Zearalenone (ZEN) is a widespread mycotoxin that contaminates crops and animal feed, causing serious health problems for livestock and humans. Biological methods, especially microbial degradation and enzyme extraction, offer promising solutions for ZEN decontamination without sacrificing nutritional quality or safety concerns.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)