Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Seung Beom Seo, Jin-Seong Hwang, Eunjung Kim, Kyujung Kim, Seokbeom Roh, Gyudo Lee, Jaewoo Lim, Byunghoon Kang, Soojin Jang, Seong Uk Son, Taejoon Kang, Juyeon Jung, Jang-Seong Kim, Keun-Hur, Tae-Su Han, Eun-Kyung Lim
Summary: Researchers have developed a simple and sensitive in vitro diagnostic system for detecting gastric cancer-related biomarkers. The system combines isothermal amplification and lateral flow biosensor to detect target miRNAs with excellent sensitivity and selectivity within 3 hours. This technology is of great importance for the early diagnosis and prognosis monitoring of gastric cancer and can also be applied to detect biomarkers related to other diseases.
Article
Nanoscience & Nanotechnology
Li-qi Liu, Fei Yin, Yu Lu, Xi-luan Yan, Ching-Chou Wu, Xia Li, Chenzhong Li
Summary: A label-free and selective fluorescence platform for miRNA detection was developed using G-quadruplex nanostring and DSN-mediated RCA. The method showed excellent selectivity for perfectly matched DNA/RNA, with a detection limit of 1.019 fM for miR-21. This tool has potential applications in evaluating miR-21 levels in cell extracts for biomedical research and clinical diagnosis.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Analytical
Ruolan Huang, Kun Liu, Chensirong Yue, Ying Wang, Zhilong Zeng, Lu Xiao, Lei Dong, Rongqin Ke, Chaoyong Yang, Dan Liu
Summary: This study developed a method for visualizing mRNA at single-molecule resolution using rolling circle amplification and streptavidin-modified alkaline phosphatase (SA-ALP) detection. The method provides highly selective and sensitive RNA detection, comparable to fluorescence-based assays. It also overcomes common background interference issues and allows for the quantification of mRNA expression from two genes in single cells and multiple cell types.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Zezhou Yang, Yu Guo, Jie Zhou, Fang Liu, Wenbin Liang, Yaqin Chai, Zhaohui Li, Ruo Yuan
Summary: In this study, an innovative fluorescent sensor was developed for precise and ultrasensitive detection and imaging of target miRNA-21. The sensor utilized a dextrous target-motivated polymerization/nicking DNA nanomachineries and a hyperbranched rolling circle amplification (HB-RCA)-assisted multiposition strand displacement reaction (SDR) signal amplification approach. The technique achieved target recycling through polymerization/nicking DNA nanomachineries and HB-RCA amplification induced by the released transformation target. HB-RCA was exploited to increase the local concentration and collision efficiency of the templates and primers, generating multiple repeated DNA sequences as initiators for SDR, which improved the signal amplification multiplier. The technique effectively distinguished target miRNA-21 and detected drug-manipulative miRNA expression level abnormities. The proposed cascade nucleic acid amplification strategy could offer a promising pathway for ultrasensitive imaging of diverse biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chao Li, Mengyang Zhou, Haoyu Wang, Jie Wang, Lin Huang
Summary: This study developed a colorimetric biosensor for the visual detection of exosomes, with dual signal amplification using rolling circle amplification (RCA). The method exhibited high sensitivity and specificity, and showed potential for clinical diagnosis in distinguishing healthy individuals from leukemia patients.
Article
Chemistry, Analytical
Yuhan Wang, Jiaxuan Xiao, Xiaona Lin, Amira Waheed, Ayyanu Ravikumar, Zhen Zhang, Yanmin Zou, Chengshui Chen
Summary: A sensitive biosensor based on rolling circle amplification (RCA) was developed for colorimetric quantification of lead ion (Pb2+). The biosensor utilized GR5 DNAzymes modified on immunomagnetic beads to capture Pb2+ and initiate an RCA for improved sensitivity. The biosensor also generated a G4-hemin DNAzyme as a colorimetric signal, which showed potential in rapid detection of heavy metals in the environment.
Article
Chemistry, Multidisciplinary
Zizhong Yang, Birong Liu, Ting Huang, Mengxu Sun, Tong Li, Wen-Jun Duan, Min-Min Li, Jin-Xiang Chen, Zong Dai, Jun Chen
Summary: This study developed a novel DNA nanowire technology for highly sensitive and highly specific imaging of miRNA in living cells. The technology demonstrated high reaction kinetics and sensitivity, and could detect miRNA as low as 51 pM. Additionally, it could accurately distinguish the miRNA expression difference between normal cells and cancer cells, and simultaneously image the total amount of multiple miRNAs.
Article
Chemistry, Analytical
Ya-ru Meng, Dandan Zhang, Xiaoran Zou, Fei Ma, Qi Kang, Chun-yang Zhang
Summary: Developed a label-free and sensitive method for NAD(+) assay using a trifunctional split dumbbell probe and ligation-triggered isothermal rolling circle amplification. The method exhibits high sensitivity with a detection limit of 85.6 fM, good selectivity, and potential applications in biological research and clinical diagnosis related to NAD(+).
Article
Biophysics
Sidan Wang, Haojia Li, Kejun Dong, Wan Shu, Jiarui Zhang, Jun Zhang, Rong Zhao, Sitian Wei, Dilu Feng, Xianjin Xiao, Wei Zhang
Summary: Point of care testing (POCT) plays a crucial role in disease diagnosis and prognosis evaluation. The biosensor based on CRISPR/Cas12a is a powerful diagnostic tool due to its high sensitivity. However, its limited recognition ability and high cost hinder its universal application. Therefore, it is important to develop a CRISPR/Cas12a-based RNA detection system that is easy to use, cheap, specific, and universal.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Linying Yu, Liping Zhu, Yao Peng, Mengting Sheng, Jianshe Huang, Xiurong Yang
Summary: A highly sensitive and versatile dual-amplification electrochemiluminescence (ECL) biosensing platform was developed for rapid and sensitive detection of biomarkers. The platform utilized DNA nanostructures and catalyzed hairpin assembly (CHA) to achieve high amplification efficiency. The biosensor demonstrated a detection limit of 1.09 fM for target DNA and showed selective detection of silver ions. The proposed strategy has potential applications in disease diagnosis and environmental monitoring.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Guang Li, Shengjie Ge, Ping Niu, Jianyou Zhang, Yu Mao, Youwei Wang, Aidong Sun
Summary: A high-throughput surface-enhanced Raman scattering-based lateral flow assay is proposed for accurate quantification of ctDNA associated with head and neck squamous cell carcinoma. The method combines high-performing SERS probes and target-specific signal amplification strategy, enabling fast and parallel detection of multiple samples.
BIOMEDICAL OPTICS EXPRESS
(2022)
Review
Chemistry, Analytical
Ju Liu, Guoyang Xie, Sidi Lv, Qin Xiong, Hengyi Xu
Summary: Isothermal nucleic acid amplification techniques are crucial diagnostic tools for clinical applications and food safety inspections. Rolling circle amplification (RCA) is a highly efficient and robust method that meets the convenient amplification requirements for point-of-care testing. This review introduces the principles, species, and optimization strategies of RCA, and summarizes recent achievements in the design, development, and application of RCA biosensors. Additionally, the versatile application of RCA in DNA nanotechnology for bioimaging, drug delivery, and therapeutics is discussed. The current challenges and future perspectives of the RCA technique are also addressed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Qian Zhang, Donge Tang, Aiyun Zha, Jingquan He, Dandan Li, Yumei Chen, Wanxia Cai, Jian Dai, Shaodong Luan, Lianghong Yin, Wei Zhang, Yong Dai
Summary: The expression of NFE2L3 is higher in renal cancerous tissues compared to normal tissues and is associated with poor prognosis in patients. Furthermore, NFE2L3 plays a role in regulating the immune microenvironment in renal cancer patients.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Immunology
Zhongji Jiang, Wei Zhang, Zhipeng Zeng, Donge Tang, Chujiao Li, Wanxia Cai, Yumei Chen, Ya Li, Qiu Jin, Xinzhou Zhang, Lianghong Yin, Xueyan Liu, Yong Xu, Yong Dai
Summary: This study reveals the important role of METTL24 in kidney renal clear cell carcinoma (KIRC), showing its potential as a prognostic marker and immune-related target for clinical treatment. METTL24 is associated with immune infiltration levels and affects the overall survival rates of KIRC patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Lizhen Ye, Tingting Fan, Ying Qin, Cheng Qiu, Lulu Li, Mengmeng Dai, Yaoyao Zhou, Yan Chen, Yuyang Jiang
Summary: miR-455-3p is up-regulated in colorectal cancer, promoting tumor growth, suppressing cell apoptosis, and stimulating cell migration. Its direct gene target is H2AFZ.
Article
Chemistry, Analytical
Yifei Chen, Lizhen Ye, Hui Chen, Tingting Fan, Cheng Qiu, Yan Chen, Yuyang Jiang
Summary: miRNA is a promising biomarker for cancer diagnosis, and the development of new detection methods for it has been a hot topic recently. In this study, we identified miR-625-5p as a potential biomarker for colorectal cancer through expression analysis and phenotypic experiments. We also developed a novel detection method based on strand displacement amplification (SDA) to monitor miR-625-5p levels, which showed good response and high specificity.
Article
Chemistry, Analytical
Hui Chen, Zhiyuan Zhuang, Yan Chen, Cheng Qiu, Ying Qin, Chunyan Tan, Ying Tan, Yuyang Jiang
Summary: A new platform based on branched rolling circle amplification and CRISPR-Cas12a was developed for the detection of multiple circulating non-coding RNAs as biomarkers for early-stage colorectal cancer diagnosis. The method showed high detection sensitivity and consistent results with the conventional RT-qPCR method. This one-pot, isothermal, and specific BRCACas platform holds promise as a rapid, adaptable, and practical diagnostic/prognostic cancer screening method.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Qiuzi Dai, Qinsheng Sun, Xiaorong Ouyang, Jinyang Liu, Liye Jin, Ahao Liu, Binsheng He, Tingting Fan, Yuyang Jiang
Summary: 1,3,5-triazine derivatives, also known as s-triazines, have shown their potential as anticancer agents. Several s-triazine derivatives have been approved for the treatment of refractory ovarian cancer, metastatic breast cancer, and leukemia. This review focuses on the medicinal chemistry of s-triazines and their targeting of topoisomerases, tyrosine kinases, phosphoinositide 3-kinases, NADP+-dependent isocitrate dehydrogenases, and cyclin-dependent kinases in various signaling pathways. It provides a comprehensive summary of the discovery, structure optimization, and biological applications of these s-triazine derivatives as anticancer drugs.
Article
Immunology
Yafang Zhong, Wei Zhang, Dongzhou Liu, Zhipeng Zeng, Shengyou Liao, Wanxia Cai, Jiayi Liu, Lian Li, Xiaoping Hong, Donge Tang, Yong Dai
Summary: In this study, seven key biomarkers were identified and validated for the potential diagnosis of Chinese SS patients using gene expression data analysis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Ning Wang, Tingting Fan, Yan Chen, Hui Chen, Ying Qin, Yuyang Jiang
Summary: Colorectal cancer (CRC) is now the third most common malignancy worldwide. Current screening methods for CRC have disadvantages such as high costs, difficult procedures, and long implementation timelines. Fecal screening for CRC offers advantages of ease of operation, noninvasiveness, cost-effectiveness, and superior sensitivity. In this study, aptamers were developed using the whole-bacterium SELEX method to specifically target Fusobacterium nucleatum (F. nucleatum), which is a crucial biomarker for early detection and prognosis of CRC. The aptamers showed high specificity and affinity, and hold promise for CRC diagnosis and prognostic prediction.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Dandan Li, Minglin Ou, Wei Zhang, Qi Luo, Wanxia Cai, Chune Mo, Wenken Liang, Guandong Dai, Lianghong Yin, Peng Zhu, Donge Tang, Yong Dai
Summary: In this study, the point mutation R286W of the CLCN7 gene in ADO2-iPSCs was successfully corrected by CRISPR/Cas9 mediated homologous recombination. The obtained gene corrected ADO2-iPSCs (GC-ADO2-iPSCs) serve as an ideal control cellular model for investigating the pathogenesis of osteopetrosis.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Article
Chemistry, Medicinal
Tianyi Wang, Ying Tan, Yu Zong Chen, Chunyan Tan
Summary: Infrared (IR) spectroscopy is a powerful tool for analyzing functional groups in organic compounds. This paper presents a new deep learning method for transforming IR spectral features into intuitive image-like feature maps and predicting major functional groups. The method successfully identifies 21 major functional groups for each molecule without expert guidance and feature selection, and it also shows potential for automated analysis in various fields.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Multidisciplinary Sciences
Zhiqian Yang, Wei Zhang, Lintai Li, Nan Hu, Xiangnan Dong, Yumei Chen, Wanxia Cai, Lianghong Yin, Fanna Liu, Donge Tang, Yong Dai
Summary: In this study, we found that METTL7A gene was downregulated in renal cancer tissues and low expression was correlated with poor prognosis. Functional enrichment analysis suggested the potential role of METTL7A in immune functions in renal cancer. Our findings indicate that METTL7A can serve as a prognostic biomarker and immunotherapy target for kidney cancer.
Article
Medicine, Research & Experimental
Yue Peng, Yixi Li, Wei Zhang, Yu ShangGuan, Ting Xie, Kang Wang, Jing Qiu, Wenjun Pu, Biying Hu, Xinzhou Zhang, Lianghong Yin, Donge Tang, Yong Dai
Summary: This study identified and characterized extrachromosomal circular DNA (eccDNA) in end-stage renal disease (ESRD) patients. The distribution and motifs of eccDNA in ESRD patients were analyzed and compared to healthy individuals. Additionally, specific eccDNA-targeting genes associated with ESRD were identified, suggesting a potential mechanism for ESRD development.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Nanoscience & Nanotechnology
Chen Li, Hui Chen, Tingting Fan, Jingru Zhao, Zheng Ding, Zeyu Lin, Shuqing Sun, Chunyan Tan, Feng Liu, Hongtao Jiang, Ying Tan
Summary: In this study, a dark-field microscopy visual single-particle detection scheme was developed to accurately evaluate telomerase activity. The method involved the attachment of telomerase substrate primer to magnetic beads, which were then hybridized with complementary DNA modified with gold nanoparticles. The telomerase activity was quantitatively measured by visually counting bright spots based on dark-field microscopy. This strategy showed excellent sensitivity and specificity and has the potential to be a powerful tool in early and noninvasive diagnosis of bladder cancer.
Article
Nanoscience & Nanotechnology
Weijie Chen, Huijuan Chen, Yurou Huang, Ying Tan, Chunyan Tan, Yuan Xie, Jun Yin
Summary: Organic fluorescent dyes with aggregation-induced emission (AIE) property have extensive applications in imaging, labeling, and adjusting microprocesses in aggregated environments. Thienoisoindigo derivatives with AIE characteristics, such as TII-TPE, have been developed and demonstrated high photostability and efficient photothermal conversion efficiency. These derivatives can be used as functional AIE structures by installing AIE blocks or other rotatable groups. The nanoparticles prepared from thienoisoindigo and tetraphenylethene (TII-TPE) and thienoisoindigo and triphenylamine (TII-TPA) showed significant inhibitory effects on tumor growth in a tumor mouse model after four treatment cycles.
ACS APPLIED BIO MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Weijie Chen, Huijuan Chen, Yurou Huang, Ying Tan, Chunyan Tan, Yuan Xie, Jun Yin
Summary: Organic fluorescent dyes with aggregation-induced emission (AIE) property have a wide range of applications in imaging, labeling, and microprocesses in aggregated environments. In this study, a thienoisoindigo derivative with AIE characteristics was developed and nanoparticles were prepared, showing significant inhibition of tumor growth.
ACS APPLIED BIO MATERIALS
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
