Journal
TALANTA
Volume 191, Issue -, Pages 46-53Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.08.015
Keywords
RF-probe; Uric acid; H2O2; Au/Ag NCs
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Funding
- National Natural Science Foundation of China [NSFC-31200317]
- Jiangsu University [11JDG1147, 14JDG051]
- Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology [2015H5004]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Jiangsu Collabrative Innovation Center of Technology and Material of Water Treatment
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In this work, a ratiometric fluorescent probe (RF-probe) for highly sensitive and selective detection of uric acid was reported for the first time toward H2O2 based on inner filter effect (IFE) between bimetallic gold/silver nanoclusters (Au/Ag NCs) and 2,3-diaminophenazine (DAP). For this RF-probe, uric acid was degraded to allantoin and H2O2. Upon the addition of HRP, o-phenylenediamine (OPD) could be catalytically oxidized to DAP in the presence of H2O2, then the fluorescence intensity corresponding to DAP at 580 nm increased dramatically with a fluorescence quenching of BSA-Au/Ag NCs at 690 nm, resulting in a RF-probe toward uric acid. This RF-probe allowed for the sensitive detection of uric acid in range of 5.0 x 10(-5) M to 5.0 x 10(-5) M with a detection limit (S/N = 3) as low as 5.1 x 10(-5) M. At the same time, it has been successfully used for uric acid levels detection in human serum, and the results are consistent with those of the hospital. RF-probe built may provide a ratiometric fluorescence universal platform for detection of various species involving in the production of H2O2 in other biological systems.
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