Article
Plant Sciences
Eleonora Spinozzi, Marta Ferrati, Desiree Lo Giudice, Eugenio Felicioni, Riccardo Petrelli, Giovanni Benelli, Filippo Maggi, Marco Cespi
Summary: Recently, microwave-assisted hydrodistillation (MAH) has been used to extract essential oils (EO) from Carlina acaulis L. (Asteraceae) with increased yield and reduced extraction time and energy costs. The optimized MAH conditions showed higher efficiency in terms of EO yield (0.65%) and extraction time (210 min) compared to traditional hydrodistillation (HD) (0.49% yield and longer extraction time). This study highlights the potential of MAH for industrial-scale EO extraction in insecticidal and acaricidal formulations, offering improved yield and reduced resource consumption.
Article
Infectious Diseases
Antonio Rosato, Alexia Barbarossa, Ahmed M. Mustafa, Giulia Bonacucina, Diego Romano Perinelli, Riccardo Petrelli, Filippo Maggi, Eleonora Spinozzi
Summary: The study evaluated the antimicrobial and antifungal activities of essential oil, carlina oxide, and nanoemulsion derived from Carlina acaulis. Results indicated that these products have potential as natural antibacterial and antifungal agents.
Article
Entomology
Roman Pavela, Lucia Pavoni, Giulia Bonacucina, Marco Cespi, Loredana Cappellacci, Riccardo Petrelli, Eleonora Spinozzi, Cristina Aguzzi, Laura Zeppa, Massimo Ubaldi, Nicolas Desneux, Angelo Canale, Filippo Maggi, Giovanni Benelli
Summary: Carlina acaulis root essential oil is an effective mosquito larvicide with carlina oxide as its main component. Formulations containing this essential oil showed high larvicidal activity against Culex quinquefasciatus while exhibiting low toxicity to human cell lines and Wistar rats. The study highlights the potential of C. acaulis essential oil microemulsion for developing safe and long-lasting larvicides.
JOURNAL OF PEST SCIENCE
(2021)
Article
Agricultural Engineering
Sara Alvarez-Rodriguez, Eleonora Spinozzi, Adela M. Sanchez-Moreiras, David Lopez-Gonzalez, Marta Ferrati, Giorgio Lucchini, Filippo Maggi, Riccardo Petrelli, Fabrizio Araniti
Summary: In this study, the phytotoxic potential of essential oil (EO) extracted from Carlina acaulis L. roots was tested against the weed Bidens pilosa L. The EO showed strong toxic effects on the plant metabolism, causing leaf necrosis, reduction in water content and leaf area, and an increase in dry weight/fresh weight ratio. It also damaged the photosynthetic machinery and altered the concentration of certain metabolites, indicating a reduction in photosynthetic efficiency. These findings suggest that C. acaulis EO has potential as a natural herbicide against weeds and requires further investigation.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Agricultural Engineering
Nickolas G. Kavallieratos, Erifili P. Nika, Anna Skourti, Eleonora Spinozzi, Marta Ferrati, Riccardo Petrelli, Filippo Maggi, Giovanni Benelli
Summary: Carlina acaulis essential oil (EO) showed high efficacy against several pests of medical and agricultural importance, including Acarus siro, Alphitobius diaperinus, and Oryzaephilus surinamensis. The EO led to high mortality rates in both adults and larvae of these pests. The efficacy of C. acaulis EO was found to be higher than that of pirimiphos-methyl, a conventional insecticide. This suggests that C. acaulis EO could be a reliable and eco-friendly alternative for pest management strategies.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Entomology
Roberto Rizzo, Marco Pistillo, Giacinto Salvatore Germinara, Gabriella Lo Verde, Milko Sinacori, Filippo Maggi, Riccardo Petrelli, Eleonora Spinozzi, Loredana Cappellacci, Valeria Zeni, Angelo Canale, Giovanni Benelli
Summary: Botanical insecticides based on Carlina acaulis essential oil and carlina oxide showed high toxicity to Bactrocera oleae, with the former exhibiting stronger electroantennographic responses. While carlina oxide did not elicit attraction or repellency responses in the olive fruit fly. This study suggests the potential use of C. acaulis products in eco-friendly lure and kill formulations for tephritid management.
Article
Plant Sciences
Eleonora Spinozzi, Marta Ferrati, Cecilia Baldassarri, Filippo Maggi, Roman Pavela, Giovanni Benelli, Cristina Aguzzi, Laura Zeppa, Loredana Cappellacci, Alessandro Palmieri, Riccardo Petrelli
Summary: Compoundsisolated from botanical sources, like carlina oxide, show great potential as bioinsecticides that can effectively target various arthropod vectors and agricultural pests while posing moderate toxicity to non-target species. In this study, the chemical synthesis of carlina oxide analogues was developed and their insecticidal activity, as well as cytotoxicity, were evaluated. The analogues showed comparable effectiveness to purified carlina oxide against houseflies and a significantly higher efficacy than carlina oxide on Culex quinquefasciatus, with a better safety profile on human keratinocyte cells. Overall, this study paves the way for the production of nature-inspired insecticides.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Plant Sciences
Radja Achiri, Lyna Benhamidat, Imane Rihab Mami, Mohammed El Amine Dib, Nadia Aissaoui, Chewki Ziani Cherif, Hocine Ziani Cherif, Alain Muselli
Summary: This study aimed to investigate the chemical composition, antioxidant, antimicrobial, and anti-inflammatory activities of essential oil and carlina oxide isolated from Carlina hispanica roots. The results indicated that carlina oxide exhibited significant antioxidant and anti-inflammatory effects, and when combined with positive controls, showed improved activities with reduced concentrations. Further research is needed to explore the potential pharmaceutical applications of these combinations.
JOURNAL OF ESSENTIAL OIL BEARING PLANTS
(2021)
Article
Agronomy
Nickolas G. Kavallieratos, Erifili P. Nika, Anna Skourti, Maria C. Boukouvala, Catherine T. Ntalaka, Filippo Maggi, Eleonora Spinozzi, Riccardo Petrelli, Diego Romano Perinelli, Giovanni Benelli, Angelo Canale, Giulia Bonacucina
Summary: The root essential oil of Carlina acaulis encapsulated in a nanoemulsion showed high toxicity against larvae of Tribolium castaneum and Tribolium confusum, but low toxicity against larvae of Tenebrio molitor. However, it exhibited high adulticidal activity against the tested species.
PEST MANAGEMENT SCIENCE
(2022)
Article
Chemistry, Analytical
Weiyi Wen, Ping Xu, Haiping Xiang, Mengyi Wen, Xuemin Ye, Chu Chu, Shengqiang Tong
Summary: In this study, a comprehensive two-dimensional countercurrent chromatography (CCC) combined with gas chromatography (GC) was used to characterize the chemical constituents of Artemisia argyi essential oil and evaluate the orthogonality of the 2D chromatographic system. A solvent system composed of n-hexane/acetonitrile/methanol (2:2:1, v/v/v) was selected for the first dimensional separation and all CCC fractions were analyzed by GC. A 2D contour plot was created to visualize the chemical compositions. A total of 1392 peaks were obtained through the comprehensive 2D CCC x GC separation and high spatial coverage and low linear correlation coefficient were achieved. Moreover, all compounds were identified by GC-MS. The obtained 2D contour plot could be divided into six zones representing different component groups, which could be used to identify and predict the structure of unknown compounds in Artemisia argyi essential oil for comprehensive characterization.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Cecilia Cagliero, Carlo Bicchi, Arianna Marengo, Patrizia Rubiolo, Barbara Sgorbini
Summary: This review provides an overview of recent advances in gas chromatography for essential oil analysis. It focuses on new stationary phases, advanced analytical methods, and instruments. The review explores the use of ionic liquids as stationary phases and strategies to speed up analysis time. It also discusses recent developments in column miniaturization and includes applications in the field of essential oils.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ambika Poudel, Noura S. Dosoky, Prabodh Satyal, Kathy Swor, William N. Setzer
Summary: The purpose of this study was to analyze the essential oil composition of Grindelia squarrosa from southern Idaho. Gas chromatographic methods showed that the essential oil of G. squarrosa var. serrulata was rich in monoterpene compounds, with alpha-pinene (21.9%), limonene (17.1%), terpinolene (10.6%), and borneol (6.5%) as the major components. The essential oil composition of G. squarrosa from Idaho was similar to that reported from specimens collected from Montana, confirming the volatile phytochemistry of plants in North America. The major components of the essential oil showed strong antimicrobial activity against respiratory and dermal pathogens, with (-)-beta-pinene exhibiting strong antibacterial activity against Streptococcus pneumoniae (MIC 39.1 μg/mL) and (-)-borneol showing strong activity against Staphylococcus aureus (MIC 78.1 μg/mL).
Article
Agronomy
Phirose Kemprai, Pranjit Kumar Bora, Siddhartha Proteem Saikia, Saikat Haldar
Summary: This study identified chavibetol as the major active compound in betel oil, which exhibited potent phytotoxicity towards wheatgrass and other weeds. The inhibitory effects were observed in both water and agar medium, with IC50 values ranging from 23.2 to 122.7 μg mL(-1). These findings highlight the potential of chavibetol as a natural herbicide for weed management.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Pranjit Kumar Bora, Jadumoni Saikia, Phirose Kemprai, Siddhartha Proteem Saikia, Dipanwita Banik, Saikat Haldar
Summary: The research found that oven-drying at 40 degrees Celsius and freeze-drying were the most effective methods for sliced rhizomes of plai or cassumunar ginger, maintaining oil yield and sensory quality. The major odorant in the essential oil, 4-terpineol, was identified as both the abundant key odorant and the major phytotoxin negatively affecting seed germination and growth of wheatgrass seeds.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Lorenzo Cucinotta, Gemma De Grazia, Paola Donato, Monica Mondello, Danilo Sciarrone, Luigi Mondello
Summary: This research aimed to evaluate the genuineness of Sicilian lemon essential oils by simultaneously evaluating the chiral and isotopic data of target terpene components. A new analytical approach was developed to reduce analysis time and resource consumption. The study also discovered different isotopic fractionation due to specific biosynthetic pathways for the enantiomers of the same chiral component.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Jedrzej Kozak, Katarzyna Tyszczuk-Rotko, Krzysztof Sztanke, Malgorzata Sztanke
Summary: A sensitive and selective sodium dodecyl sulfate-modified screen-printed carbon sensor (SPCE/SDS) was used for the quantitative analysis of 3-(4-Nitrophenyl)-8-(2,3-dimethylphenyl)-7,8-dihydroimidazo[2,1-c][1,2,4]triazin-4(6H)-one (NDIT). The sensor showed excellent behavior in the electrochemical reduction of NDIT and had a wide linear range and low detection limit. It was successfully applied to the analysis of spiked human serum samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jedrzej Kozak, Katarzyna Tyszczuk-Rotko, Radovan Metelka
Summary: In this paper, a highly sensitive voltammetric sensor for the determination of the anti-cancer antibiotic bleomycin (BLM) was developed. This sensor is based on a screen-printed carbon sensor that is electrochemically pretreated and decorated with lead nanoparticles in the sample solution. The sensor exhibited low limits of detection and quantification, a wide linear range, and high sensitivity. It was successfully applied for the determination of BLM in wastewater and human urine samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Urszula Doboszewska, Katarzyna Socala, Mateusz Pierog, Dorota Nieoczym, Jan Sawicki, Adam Sajnog, Bernadeta Szewczyk, Katarzyna Mlyniec, Ireneusz Sowa, Danuta Baralkiewicz, Piotr Wlaz
Summary: The G-protein coupled receptor 39 (GPR39) is a potential target for future drugs, and its pharmacology is not fully understood. Zinc acts as an endogenous agonist or an allosteric modulator for GPR39, while TC-G 1008 is a synthetic agonist. Zinc also enhances the activity of TC-G 1008 at GPR39. Activation of GPR39 by TC-G 1008 promotes epileptogenesis in an epilepsy model and decreases seizure threshold in a seizure test. The amount of zinc in the diet contributes to the effects of TC-G 1008 in vivo.
Article
Nutrition & Dietetics
Piotr Bawiec, Jan Sawicki, Paulina Lasinska-Pracuta, Marcin Czop, Ireneusz Sowa, Katarzyna Ilowiecka, Wojciech Koch
Summary: Bioavailability refers to the fraction of a substance that is absorbed from the gastrointestinal tract and enters the bloodstream. This study aimed to assess the bioavailability of selenium (Se) from dietary supplements and explore the influence of diet type on relative bioavailability. The research used a two-stage in vitro digestion model to simulate the digestion of food containing dietary supplements. Selenium bioavailability ranged from 19.31% to 66.10% in the presence of food matrix. Sodium selenate had the highest bioavailability, followed by organic forms and sodium selenite. The basic diet with moderate protein and high carbohydrate and fiber content positively influenced Se bioavailability. The pharmaceutical form of the product also affected bioavailability, with tablets having the highest bioavailability, followed by capsules and coated tablets.
Article
Biochemistry & Molecular Biology
Ireneusz Sowa, Jaroslaw Moldoch, Roman Paduch, Maciej Strzemski, Jacek Szkutnik, Katarzyna Tyszczuk-Rotko, Slawomir Dresler, Dariusz Szczepanek, Magdalena Wojciak
Summary: This study investigated the polyphenolic composition of Carlina acaulis and demonstrated its anti-cancer potential. The plant was found to be rich in chlorogenic acids and flavonoids. Further fractionation revealed that subfractions containing fatty acids had significant inhibitory effects on cancer cells. The research highlighted the promising attributes of C. acaulis and called for further exploration of its biological activity.
Article
Biochemistry & Molecular Biology
Martyna Zagorska-Dziok, Aleksandra Ziemlewska, Agnieszka Mokrzynska, Zofia Niziol-Lukaszewska, Magdalena Wojciak, Ireneusz Sowa
Summary: This study assessed the biological activity of extracts and hydrogels containing Cornus mas L. extracts. The results showed that these extracts and hydrogels had strong antioxidant properties, positively affected the viability of skin cells, stimulated cell migration, inhibited collagenase and elastase activity, and increased skin hydration.
Article
Chemistry, Physical
Magdalena Greluk, Marek Rotko, Grzegorz Slowik, Sylwia Turczyniak-Surdacka, Gabriela Grzybek, Katarzyna Tyszczuk-Rotko
Summary: The promotional effect of potassium on a Co/MnOx catalyst in the steam reforming of ethanol (SRE) process was investigated. The addition of potassium significantly improved the stability and ability for C-C bond cleavage of the catalyst. The enhancement in activity, selectivity to hydrogen and carbon dioxide production was attributed to the influence of the alkali promoter on the reducibility of cobalt and manganese oxides. Microscopic observations showed that the formation of fibrous carbon deposits led to the deactivation of the catalysts under SRE conditions, but the potassium-promoted catalyst exhibited lower carbon accumulation compared to the unpromoted sample.
Article
Chemistry, Physical
Katarzyna Tyszczuk-Rotko, Damian Gorylewski, Rafal Olchowski, Ryszard Dobrowolski
Summary: This paper presents a new electrode material called diclofenac-impregnated mesoporous carbon modified with a cationic surfactant, which is used for ultratrace analysis of the arsenic drug roxarsone. The modified material showed excellent sensitivity and a wide linear range for detecting the drug in water samples without complicated pretreatment steps. This novel electrode material has great potential for environmental analysis of arsenic drugs.
Article
Chemistry, Physical
Jedrzej Kozak, Katarzyna Tyszczuk-Rotko, Aleksy Keller, Magdalena Wojciak, Ireneusz Sowa
Summary: The purpose of this study was to develop a simple, fast, and sensitive method for determining the polyphenolic antioxidant curcumin. By electrochemically activating a screen-printed boron-doped diamond electrode, the analytical signal of curcumin was significantly amplified. Under optimized conditions, the method achieved a very low detection limit and yielded consistent results compared to other commonly used methods.
Review
Medicine, General & Internal
Jaromir Jarecki, Marcin Krzysztof Wasko, Wojciech Widuchowski, Agnieszka Tomczyk-Warunek, Magdalena Wojciak, Ireneusz Sowa, Tomasz Blicharski
Summary: This review provides an overview of the commonly used clinical methods for treating articular cartilage defects, with a specific emphasis on recent clinical trials. Currently, no single method fully satisfies the requirements for effective cartilage healing while remaining easily applicable during surgical procedures. However, there are several available options and the choice of treatment should take into consideration factors such as the location and size of the cartilage lesion, patient preferences, and whether it is chondral or osteochondral in nature. Promising future directions include tissue engineering, stem cell therapies, and the development of pre-formed scaffolds from hyaline cartilage, offering hope for improved outcomes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Oncology
Agata Malek, Maciej Strzemski, Joanna Kurzepa, Jacek Kurzepa
Summary: This paper presents the current state of knowledge regarding the mechanisms of anticancer activity of bee venom, showing its strong potential in inducing apoptosis and inhibiting the cell cycle of tumor cells without affecting normal cells significantly. Animal studies also support the safety of venom doses effective in vitro. This information is valuable for planning future clinical trials.
Article
Biochemistry & Molecular Biology
Ireneusz Sowa, Roman Paduch, Jaroslaw Moldoch, Dariusz Szczepanek, Jacek Szkutnik, Pawel Sowa, Katarzyna Tyszczuk-Rotko, Tomasz Blicharski, Magdalena Wojciak
Summary: This study assessed the cytotoxic potential of Carlina vulgaris against human colorectal adenocarcinoma and identified several compounds linked to this effect. One of the compounds exhibited significant cytotoxicity, although without selectivity. The cytotoxicity was associated with the generation of intracellular reactive oxygen species.
Article
Biochemistry & Molecular Biology
Martyna Zagorska-Dziok, Aleksandra Ziemlewska, Agnieszka Mokrzynska, Zofia Niziol-Lukaszewska, Ireneusz Sowa, Dariusz Szczepanek, Magdalena Wojciak
Summary: The properties of extracts and ferments from Cornus mas L. were compared for their potential use in cosmetic products. The study evaluated their composition, antioxidant properties, cytotoxicity, collagenase and elastase inhibitory activity, and antimicrobial properties. Both the extract and ferment showed antioxidant and anti-aging effects, making them promising ingredients in skin care preparations. The stronger activity of the ferment suggested the legitimacy of utilizing kombucha fermentation process for valuable cosmetic products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Jaromir Jarecki, Bartosz Potoczniak, Artur Dziedzic, Teresa Malecka-Masalska, Tomasz Skrzypek, Waldemar Kazimierczak, Marcin Skowronek, Magdalena Wojciak, Slawomir Dresler, Marcin Wasko, Ireneusz Sowa
Summary: This study assessed the relationship between the severity of knee osteoarthritis (KOA) and body composition in postmenopausal women using bioimpedance analysis (BIA). The results showed that overweight and obesity in postmenopausal women are primarily associated with adipose tissue level and its metabolic activity.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Peripheral Vascular Disease
Marcin Feldo, Magdalena Wojciak, Ireneusz Sowa, Stanislaw Przywara, Pawel Sowa, Roman Paduch
Summary: Diosmin and diosmetin have different impacts on pro-inflammatory factors depending on the inflammation inducer. Diosmetin more effectively modulated inflammation than diosmin, suggesting that the attachment of the sugar moiety attenuates its activity.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
